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1.
Arch Virol ; 166(9): 2495-2504, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34232400

RESUMO

Short beak and dwarfism syndrome (SBDS) emerged in Cherry Valley duck flocks in China in 2015, and novel goose parvovirus (NGPV) was shown to be the etiological agent of SBDS. To date, it is not known whether SBDS-related NGPV isolates possess common molecular characteristics. In this study, three new NGPV strains (namely, SDHT16, SDJN19, and SDLC19) were isolated from diseased ducks showing typical signs of SBDS and successfully passaged in embryonated goose or Cherry Valley duck eggs. The complete genome sequences of these NGPV strains were 98.9%-99.7% identical to each other but showed slightly less similarity (95.2%-96.1% identity) to classical GPV strains. A total of 16 common amino acid substitutions were present in the VP1 proteins of six NGPV strains (SDHT16, SDJN19, SDLC19, QH, JS1, and SDLC01) compared with the classical Chinese GPV strains, nine of which were identical to those found in European GPV strain B. The non-structural protein Rep1 of the six NGPV strains had 12 common amino acid substitutions compared with the classical GPV strains. Phylogenetic analysis indicated that the Chinese NGPV strains clustered with the European SBDS-related NGPV strains, forming a separate branch that was distinct from the group formed by the classical GPV strains. The present study shows the common molecular characteristics of NGPV isolates and suggests that the Chinese NGPV isolates probably share a common ancestor with European SBDS-related NGPV strains.


Assuntos
Nanismo/veterinária , Nanismo/virologia , Parvovirinae/classificação , Parvovirinae/genética , Filogenia , Doenças das Aves Domésticas/virologia , Animais , China , Patos/virologia , Gansos/virologia , Genoma Viral , Infecções por Parvoviridae/virologia , Parvovirus/genética , Alinhamento de Sequência , Sequenciamento Completo do Genoma
3.
Equine Vet J ; 53(5): 886-894, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34101906

RESUMO

Equine parvovirus hepatitis (EqPV-H) was first described in 2018 in a fatal case of Theiler's disease which followed the administration of an equine-origin biological product. The virus has since been frequently identified in serum and liver tissue of horses affected by Theiler's disease-an acute, severe hepatitis characterised by fulminant hepatic necrosis with a fatal outcome in most cases. EqPV-H is hepatotropic, appears to be associated with subclinical to severe hepatitis in horses, and is a likely cause of Theiler's disease. Although this disease is most frequently reported following the administration of equine-origin biological products, it can also occur among in-contact horses. Horizontal transmission may be iatrogenic, via contaminated equine-origin biological products such as equine serum, botulism or tetanus antitoxin, and mesenchymal stem cells or by means of the oral route of infection. Other horizontal transmission routes, for example, arthropod vectors, warrant further investigation. A worldwide prevalence of EqPV-H antibodies and DNA has been reported in asymptomatic horses. EqPV-H-positive horses suffering from acute, severe hepatitis have reportedly developed clinical signs including icterus, lethargy, inappetence, and neurological abnormalities and have had increased liver-associated biochemistry parameters recorded. The most common histopathological abnormalities of the liver have been hepatocellular necrosis, collapse of the lobular architecture, and lymphocytic infiltration. Most horses infected experimentally with EqPV-H have developed subclinical hepatitis, and close temporal associations between peak viraemia, seroconversion, and the onset of hepatitis have been observed. Based on strong evidence indicating that EqPV-H causes hepatitis in horses, veterinarians should consider this virus an important differential diagnosis in such cases. Potential risks associated with the administration of equine-origin biological products must be emphasised.


Assuntos
Hepatite Viral Animal , Hepatite , Doenças dos Cavalos , Infecções por Parvoviridae , Parvovirus , Animais , Cavalos , Infecções por Parvoviridae/veterinária
4.
Arch Virol ; 166(7): 2011-2016, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34080052

RESUMO

Recently, a novel duck-origin goose parvovirus (N-GPV) was reported to cause short beak and dwarfism syndrome in ducks. In this study, we performed complete genome sequencing and analyzed three different duck-derived parvoviruses that infected different breeds of ducks. Phylogenetic trees based on gene sequences indicated that they were classical goose parvovirus (C-GPV), Muscovy duck parvovirus (MDPV), and N-GPV. Furthermore, potential recombination events were found. These results improve our understanding of the diversity of duck-derived parvoviruses in Anhui province, eastern China, and provide a reference for the prevention of associated diseases.


Assuntos
Patos/virologia , Infecções por Parvoviridae/virologia , Parvovirinae/genética , Parvovirus/genética , Animais , Bico/virologia , China , Filogenia , Doenças das Aves Domésticas/virologia , Análise de Sequência de DNA
5.
Poult Sci ; 100(8): 101251, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34175799

RESUMO

Previously, we isolated a novel strain of goose pegivirus (GPgV) that infects geese and shows high levels of lymphotropism. This novel pegivirus strain is phylogenetically distinct from previously known Pegivirus species, Pegivirus A-K, and qualifies as a candidate new Pegivirus species, GPgV. GPgV is tentatively named Pegivirus M. Here, to better understand the epidemic of GPgV infection and the coinfection of this virus with other viruses in Southwest China, 25 geese in poor health from Sichuan Province and 24 geese in poor health from the municipality of Chongqing were collected. The geese were tested for 9 types of goose viruses (goose hemorrhagic polyomavirus, GPgV, astrovirus, parvovirus, circovirus, reovirus, coronavirus, paramyxovirus, and avian influenza virus) by RT-PCR or nested RT-PCR. GPgV RNA was detected in 2 out of 25 monoinfections and 8 out of 25 coinfections with other viruses on Sichuan farms and 2 out of 24 monoinfections and 10 out of 24 coinfections on Chongqing farms. Overall, 22 of the 49 (44.9%) geese were positive for GPgV, which indicated a high infection rate. To the best of our knowledge, this is the first report of GPgV coinfection with other epidemic viruses. This study enhances our understanding of the emergence and epidemiology of Pegivirus.


Assuntos
Circovirus , Coinfecção , Parvovirus , Doenças das Aves Domésticas , Animais , Galinhas , China/epidemiologia , Coinfecção/epidemiologia , Coinfecção/veterinária , Gansos , Parvovirus/genética , Pegivirus , Filogenia , Doenças das Aves Domésticas/epidemiologia
6.
Arch Virol ; 166(7): 1931-1942, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33934195

RESUMO

Since its first recognition in the early 1960s, Derzsy's disease has caused significant economic losses in the goose meat industry through the world. Today, Derzsy's disease still maintains its importance for small-scale waterfowl farming, despite not having a significant impact on public health. In the present study, we investigated the distribution of goose parvovirus (GPV) and its potential variants from a 2019 outbreak in Turkey. Tissue samples were obtained from infected eggs and goslings that were raised in distinct farming areas of the various provinces. For this purpose, a novel primer set for amplification of a 630-bp region of VP3 was designed to confirm GPV infection by conventional PCR method. A 4709-base nucleotide sequence including the structural, non-structural, and 5' inverted terminal repeat regions was obtained from three samples from the Central Anatolian region. Multiple sequence comparisons and phylogenetic analysis demonstrated that the field strains clustered with European group 2 and contained a series of unique amino acid substitutions that might affect the virulence of the virus. These results confirmed that European-related field strains caused the outbreak in Asia Minor, and this might assist in understanding the circulation of GPV in Asia and Europe.


Assuntos
Gansos/virologia , Parvovirinae/genética , Parvovirus/genética , Virulência/genética , Substituição de Aminoácidos/genética , Animais , Ásia , Surtos de Doenças , Europa (Continente) , Infecções por Parvoviridae/virologia , Filogenia , Doenças das Aves Domésticas/virologia
7.
Viruses ; 13(3)2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33804173

RESUMO

Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0-6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries.


Assuntos
DNA Viral/genética , Gastroenteropatias/virologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Parvovirus/genética , Doenças Respiratórias/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Estudos de Coortes , DNA Viral/análise , Fezes/virologia , Feminino , Finlândia/epidemiologia , Gastroenteropatias/epidemiologia , Humanos , Lactente , Letônia/epidemiologia , Malaui/epidemiologia , Masculino , Pessoa de Meia-Idade , Nasofaringe/virologia , Infecções por Parvoviridae/sangue , Parvovirus/classificação , Filogenia , Doenças Respiratórias/sangue , Doenças Respiratórias/epidemiologia , Adulto Jovem
8.
BMC Vet Res ; 17(1): 96, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33648493

RESUMO

BACKGROUND: Mesenchymal stem cells (MSCs) have generated a great amount of interest in recent years as a novel therapeutic application for improving the quality of pet life and helping them free from painful conditions and diseases. It has now become critical to address the challenges related to the safety and efficacy of MSCs expanded in vitro. In this study, we establish a standardized process for manufacture of canine adipose-derived MSCs (AD-MSCs), including tissue sourcing, cell isolation and culture, cryopreservation, thawing and expansion, quality control and testing, and evaluate the safety and efficacy of those cells for clinical applications. RESULTS: After expansion, the viability of AD-MSCs manufactured under our standardized process was above 90 %. Expression of surface markers and differentiation potential was consistent with ISCT standards. Sterility, mycoplasma, and endotoxin tests were consistently negative. AD-MSCs presented normal karyotype, and did not form in vivo tumors. No adverse events were noted in the case treated with intravenously AD-MSCs. CONCLUSIONS: Herein we demonstrated the establishment of a feasible bioprocess for manufacturing and banking canine AD-MSCs for veterinary clinical use.


Assuntos
Tecido Adiposo/citologia , Transplante de Células-Tronco Mesenquimais/veterinária , Células-Tronco Mesenquimais/citologia , Bancos de Tecidos , Animais , Testes de Carcinogenicidade , Técnicas de Cultura de Células/veterinária , Separação Celular/veterinária , Criopreservação/veterinária , Cães , Feminino , Leucopenia/veterinária , Masculino , Camundongos SCID , Infecções por Parvoviridae/terapia , Infecções por Parvoviridae/veterinária , Parvovirus , Controle de Qualidade
9.
PLoS One ; 16(3): e0247266, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33651823

RESUMO

Carnivore protoparvovirus-1 (CPPV-1), a viral species containing feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants, are widely spread among domestic and wild carnivores causing systemic fatal diseases. Wild fishing cats (Prionailurus viverrinus), a globally vulnerable species, have been found dead. Postmortem examination of the carcasses revealed lesions in intestine, spleen and kidney. CPPV-1 antigen identification in these tissues, using polymerase chain reaction (PCR) and immunohistochemistry (IHC), supported the infection by the virus. PCR- and IHC-positivity in kidney tissues revealed atypical localization of the virus while in situ hybridization (ISH) and transmission electron microscopy (TEM) with the pop-off technique confirmed the first description of viral localization in kidneys. Complete genome characterization and deduced amino acid analysis of the obtained CPPV-1 from the fishing cats revealed FPV as a causative agent. The detected FPV sequences showed amino acid mutations at I566M and M569R in the capsid protein. Phylogenetic and evolutionary analyses of complete coding genome sequences revealed that the fishing cat CPPV-1 genomes are genetically clustered to the FPV genomes isolated from domestic cats in Thailand. Since the 1970s, these genomes have also been shown to share a genetic evolution with Chinese FPV strains. This study is the first evidence of CPPV-1 infection in fishing cats and it is the first to show its localization in the kidneys. These findings support the multi-host range of this parvovirus and suggest fatal CPPV-1 infections may result in other vulnerable wild carnivores.


Assuntos
Felidae/virologia , Vírus da Panleucopenia Felina/genética , Vírus da Panleucopenia Felina/patogenicidade , Animais , Animais Selvagens/virologia , Evolução Biológica , Proteínas do Capsídeo/genética , Carnívoros/genética , Gatos , Evolução Molecular , Panleucopenia Felina/virologia , Vírus da Panleucopenia Felina/isolamento & purificação , Especificidade de Hospedeiro , Rim/patologia , Rim/virologia , Mutação , Infecções por Parvoviridae/virologia , Parvovirus/genética , Parvovirus Canino/genética , Filogenia , Reação em Cadeia da Polimerase/veterinária , Tailândia
10.
J Wildl Dis ; 57(1): 234-237, 2021 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-33635978

RESUMO

Using PCR, we evaluated the presence of parvoviruses and Mycoplasma spp. in 123 American mink (Neovison vison), an introduced invasive carnivore in Chile. Our results showed all analyzed animals were negative for both pathogen groups. We cannot completely dismiss their presence, but if present, their prevalence should be lower than 2%.


Assuntos
Espécies Introduzidas , Vison , Infecções por Mycoplasma/veterinária , Mycoplasma/isolamento & purificação , Infecções por Parvoviridae/veterinária , Parvovirus/isolamento & purificação , Animais , Chile , Reservatórios de Doenças/veterinária , Infecções por Mycoplasma/epidemiologia , Infecções por Mycoplasma/microbiologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia
11.
Vet Res Commun ; 45(1): 31-40, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33392909

RESUMO

Gastrointestinal disorders caused by enteric viruses are frequently reported in dogs worldwide, with significant mortality rates in unvaccinated individuals. This study reports the identification and molecular characterization of Canine parvovirus (CPV-2), Canine coronavirus (CcoV), Canine astrovirus (AstV), and Canine calicivirus (CcaV) in a panel of dogs showing severe enteric clinical signs sampled in a typical Mediterranean environment (Sardinia, Italy). At least one of these viral species was detected in 92.3% samples. CPV-2 was the most frequently detected virus (87.2%), followed by AsTv (20.5%), CCoV-IIa (18%), and CCoV-I (10.3%). CCoV-IIb and CaCV were not detected in any sample. Single infection was detected in 24 samples (66.7%), mainly related to CPV-2 (91.7%). Coinfections were present in 33.3% samples with constant detection of CPV-2. Canine coronavirus was present only in coinfected animals. The VP2 sequence analysis of CPV-2 positive samples confirmed the presence of all variants, with CPV-2b most frequently detected. Phylogeny based on the CcoV-IIa spike protein (S) gene allowed to identify 2 different clades among Sardinian isolates but failed to distinguish enteric from pantropic viruses. Study on presence and prevalence of enteroviruses in dogs increase our knowledge about the circulation of these pathogens in the Mediterranean area and highlight the need for dedicated routine vaccine prophylaxis. Molecular analyses of enteric viruses are fundamental to avoid failure of vaccines caused by frequent mutations observed in these enteroviruses.


Assuntos
Infecções por Astroviridae/veterinária , Infecções por Caliciviridae/veterinária , Infecções por Coronavirus/veterinária , Doenças do Cão/virologia , Infecções por Parvoviridae/veterinária , Animais , Astroviridae/genética , Astroviridae/isolamento & purificação , Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/virologia , Caliciviridae/genética , Caliciviridae/isolamento & purificação , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Coronavirus/genética , Coronavirus/isolamento & purificação , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , DNA Viral/isolamento & purificação , Doenças do Cão/epidemiologia , Cães , Fezes/virologia , Feminino , Itália/epidemiologia , Masculino , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Parvovirus/genética , Parvovirus/isolamento & purificação , Filogenia
12.
Arch Virol ; 166(1): 231-236, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33136208

RESUMO

In this study, a novel parvovirus (gyb-MR02/2015/HUN, MT580795) was detected in barn owls (Tyto alba) and genetically characterized using viral metagenomics and PCR methods. The NS1 and VP1 proteins of gyb-MR02/2015/HUN share only 45.4% and 50.1% amino acid sequence identity, respectively, to the corresponding proteins of peafowl parvovirus 2 (MK988620), the closest relative. Out of 11 faecal specimens from owls (six from little owls, three from barn owls, and two from long-eared owls), two barn owl samples were positive for the novel parvovirus, which is distantly related to members of the recently established genus Chaphamaparvovirus in the subfamily Hamaparvovirinae. Systematic investigation is necessary to explore the diversity of parvoviruses.


Assuntos
Parvovirus/genética , Estrigiformes/virologia , Animais , Hungria , Infecções por Parvoviridae/virologia
13.
J Appl Microbiol ; 131(1): 499-512, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33325600

RESUMO

AIMS: To develop a protocol for environmental sampling to detect parvoviruses of dogs and cats in the environment. METHODS AND RESULTS: Environmental contamination was carried out using different dilutions of parvovirus-contaminated materials; further field samplings were performed in areas in which clinical cases of parvovirus infections were present. Sterile cotton swabs and sponges for microbial surface sampling were used. Viruses were detected in these samples with different methods: conventional PCR, nested PCR and real-time PCR, detecting viral DNA; virus isolation, detecting infectious virus; and a commercial rapid enzyme immunoassay, detecting viral antigen. No substantial differences were observed in the two sampling methods, although the sponge was more convenient for sampling rough surfaces. Molecular assays were the most sensitive methods, identifying even very low amounts of viral DNA (up to 10 copies of viral DNA/10 µl of sample). Virus isolation and the rapid test detected the viruses only at the highest viral concentrations, both in the experimental setting and field conditions. CONCLUSIONS: Environmental sampling and molecular protocols were effective in detecting environmental contamination with parvoviruses. SIGNIFICANCE AND IMPACT OF THE STUDY: The protocol will be useful to identify possible sources of infection and to assess the efficacy of disinfection protocols in the environment.


Assuntos
Doenças do Gato/virologia , Doenças do Cão/virologia , Microbiologia Ambiental , Infecções por Parvoviridae/veterinária , Parvovirus/isolamento & purificação , Animais , Antígenos Virais/imunologia , Gatos , DNA Viral/genética , Cães , Ensaio de Imunoadsorção Enzimática , Infecções por Parvoviridae/virologia , Parvovirus/genética , Parvovirus/imunologia , Reação em Cadeia da Polimerase
14.
Vet Microbiol ; 252: 108949, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33338948

RESUMO

Bovine viral diarrhea viruses (BVDV) are significant pathogens of cattle, leading to losses associated with reproductive failure, respiratory disease and immune dysregulation. While cattle are the reservoir for BVDV, a wide range of domestic and wild ruminants are susceptible to infection and disease caused by BVDV. Samples from four American bison (Bison bison) from a captive herd were submitted for diagnostic testing due to their general unthriftiness. Metagenomic sequencing on pooled nasal swabs and serum identified co-infection with a BVDV and a bovine bosavirus. The BVDV genome was more similar to the vaccine strain Oregon C24 V than to other BVDV sequences in GenBank, with 92.7 % nucleotide identity in the open reading frame. The conserved 5'-untranslated region was 96.3 % identical to Oregon C24 V. Bosavirus has been previously identified in pooled fetal bovine serum but its clinical significance is unknown. Sequencing results were confirmed by virus isolation and PCR detection of both viruses in serum and nasal swab samples from two of the four bison. One animal was co-infected with both BVDV and bosavirus while separate individuals were positive solely for BVDV or bosavirus. Serum and nasal swabs from these same animals collected 51 days later remained positive for BVDV and bosavirus. These results suggest that both viruses can persistently infect bison. While the etiological significance of bosavirus infection is unknown, the ability of BVDV to persistently infect bison has implications for BVDV control and eradication programs. Possible synergy between BVDV and bosavirus persistent infection warrants further study.


Assuntos
Anticorpos Antivirais/sangue , Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Vírus da Diarreia Viral Bovina/imunologia , Infecções por Parvoviridae/veterinária , Parvovirus/imunologia , Animais , Bison , Doença das Mucosas por Vírus da Diarreia Viral Bovina/epidemiologia , Bovinos , Coinfecção/veterinária , Vírus da Diarreia Viral Bovina/isolamento & purificação , Infecções por Parvoviridae/microbiologia , Parvovirus/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Estados Unidos/epidemiologia
15.
PLoS Pathog ; 16(10): e1009002, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33064772

RESUMO

The autonomous parvovirus Minute Virus of Mice (MVM) localizes to cellular DNA damage sites to establish and sustain viral replication centers, which can be visualized by focal deposition of the essential MVM non-structural phosphoprotein NS1. How such foci are established remains unknown. Here, we show that NS1 localized to cellular sites of DNA damage independently of its ability to covalently bind the 5' end of the viral genome, or its consensus DNA binding sequence. Many of these sites were identical to those occupied by virus during infection. However, localization of the MVM genome to DNA damage sites occurred only when wild-type NS1, but not its DNA-binding mutant was expressed. Additionally, wild-type NS1, but not its DNA binding mutant, could localize a heterologous DNA molecule containing the NS1 binding sequence to DNA damage sites. These findings suggest that NS1 may function as a bridging molecule, helping the MVM genome localize to cellular DNA damage sites to facilitate ongoing virus replication.


Assuntos
Dano ao DNA , Vírus Miúdo do Camundongo/genética , Vírus Miúdo do Camundongo/metabolismo , Proteínas não Estruturais Virais/metabolismo , Animais , Linhagem Celular , Replicação do DNA , DNA Viral/genética , Proteínas de Ligação a DNA/genética , Feminino , Genoma Viral , Humanos , Masculino , Camundongos , Infecções por Parvoviridae/genética , Infecções por Parvoviridae/virologia , Parvovirus/genética , Replicação Viral
16.
Microb Pathog ; 149: 104485, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32926999

RESUMO

Canine parvovirus (CPV) enteritis is an important cause of morbidity and mortality in puppies despite aggressive treatment. Identification of reliable biomarkers for CPV enteritis is essential to determine the severity, duration of hospitalization, and predict the clinical outcome. Meanwhile, the biomarkers will assist in decision-making with clients about the further course of treatment or euthanasia. The present study was conducted to evaluate the changes of total leukocyte count (TLC), neutrophil count, and serum concentrations of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), intestinal fatty acid binding protein-2 (IFABP-2), albumin, ceruloplasmin (Cp), cortisol, free triiodothyronine (FT3) and free thyroxine (FT4) in survivors and non-survivors as a predictor of the clinical outcome. Marked leukopenia, neutropenia, hypoalbuminemia, elevated levels of CK-MB, IFABP-2, Cp, and cortisol were noticed in CPV-infected dogs than healthy dogs but, LDH, FT3 and FT4 concentrations did not differ significantly. The CPV-infected non-survivors had persistent leukopenia, neutropenia and elevated CK-MB, IFABP-2, Cp and cortisol concentrations at 72 h of commencement of treatment. In CPV-infected survivors, TLC and neutrophil count were significantly increased, and CK-MB, IFABP-2, Cp and cortisol concentrations were significantly decreased at 72 h of commencement of treatment. The positive predictive values (PPVs) for survival using cut-off value of TLC (>3.2 × 103/µL), neutrophil count (>1.65 × 103/µL), CK-MB (≤234.50 U/L), IFABP-2 (≤7.61 ng/mL), Cp (≤0.605 g/L) and cortisol (≤16.90 ng/mL) were determined as 89.47%, 88.88%, 94.73%, 93.33%, 94.44% and 89.47%, respectively with better area under receiver operating characteristic (ROC) curve as well as sensitivity. The magnitude of decrease in TLC, neutrophil count, and increase in CK-MB, IFABP-2, Cp and cortisol concentrations at 72 h of initiation of treatment in dogs with parvoviral enteritis could be useful indicators for the prognosis of the disease. Based on sensitivity (%) and specificity (%) from ROC curve analysis and PPV (%), it is concluded that serum CK-MB concentration will serve as the most useful biomarker followed by Cp and absolute neutrophil count.


Assuntos
Doenças do Cão , Enterite , Infecções por Parvoviridae , Parvovirus Canino , Parvovirus , Animais , Biomarcadores , Doenças do Cão/diagnóstico , Doenças do Cão/tratamento farmacológico , Cães , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/veterinária
17.
Vet Pathol ; 57(5): 706-713, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32880233

RESUMO

Carnivore protoparvovirus-1 (CPPV-1) infection has been reported frequently in both domestic and wildlife species including wild carnivores. Fifty-five captive small Indian civets (Viverricula indica), farmed for perfume production in Eastern Thailand, showed clinical signs of acute bloody diarrhea, anorexia, vomiting, circling, and seizures. The disease spread within the farm and resulted in the death of 38 of the 55 civets (69% mortality) within a month. Fecal swabs were collected from the 17 surviving civets, and necropsy was performed on 7 of the dead civets. Pathologic findings were severe hemorrhagic gastroenteritis with generalized lymphadenopathy. CPPV-1 was identified in both fecal swabs and postmortem samples by species-specific polymerase chain reaction. Further whole-gene sequencing and restriction fragment length polymorphism analysis suggested feline panleukopenia virus (FPV) as the causative agent. The viral tropism and tissue distribution were confirmed by immunohistochemistry, with immunolabeling in the cytoplasm and nucleus of small intestinal crypt epithelial cells, villous enterocytes, histiocytes in lymphoid tissues, myenteric nerve plexuses, and cerebral and cerebellar neurons. Phylogenetic analysis of civet-derived CPPV-1 indicated a genetic similarity close to the FPV HH-1/86 strain detected in a jaguar (Panthera onca) in China. To our knowledge, this mass die-off of civets is the first evidence of disease associated with CPPV-1 infection in the subfamily Viverrinae. These findings support the multi-host range of parvovirus infection and raises awareness for CPPV-1 disease outbreaks in wildlife species.


Assuntos
Surtos de Doenças/veterinária , Gastroenterite/veterinária , Hemorragia/veterinária , Infecções por Parvoviridae/veterinária , Parvovirus/isolamento & purificação , Viverridae/virologia , Animais , Carnívoros , Vírus da Panleucopenia Felina/genética , Vírus da Panleucopenia Felina/isolamento & purificação , Gastroenterite/epidemiologia , Gastroenterite/patologia , Gastroenterite/virologia , Hemorragia/patologia , Hemorragia/virologia , Especificidade de Hospedeiro , Imuno-Histoquímica/veterinária , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/patologia , Infecções por Parvoviridae/virologia , Parvovirus/genética , Filogenia , Reação em Cadeia da Polimerase/veterinária , Especificidade da Espécie , Tailândia/epidemiologia
18.
PLoS Pathog ; 16(9): e1008765, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32970777

RESUMO

Tilapia is one of the most important economic and fastest-growing species in aquaculture worldwide. In 2015, an epidemic associated with severe mortality occurred in adult tilapia in Hubei, China. The causative pathogen was identified as Tilapia parvovirus (TiPV) by virus isolation, electron microscopy, experimental challenge, In situ hybridization (ISH), indirect immunofluorescence (IFA), and viral gene sequencing. Electron microscopy revealed large numbers of parvovirus particles in the organs of diseased fish, including kidney, spleen, liver, heart, brain, gill, intestine, etc. The virions were spherical in shape, non-enveloped and approximately 30nm in diameter. The TiPV was isolated and propagated in tilapia brain cells (TiB) and induced a typical cytopathic effect (CPE) after 3 days post-infection (dpi). This virus was used to experimentally infect adult tilapia and clinical disease symptoms similar to those observed naturally were replicated. Additionally, the results of ISH and IFA showed positive signals in kidney and spleen tissues from TiPV-infected fish. To identify TiPV-specific sequences, the near complete genome of TiPV was obtained and determined to be 4269 bp in size. Phylogenetic analysis of the NS1 sequence revealed that TiPV is a novel parvovirus, forms a separate branch in proposed genus Chapparvovirus of Parvoviridae. Results presented here confirm that TiPV is a novel parvovirus pathogen that can cause massive mortality in adult tilapia. This provides a basis for the further studies to define the epidemiology, pathology, diagnosis, prevention and treatment of this emerging viral disease.


Assuntos
Doenças dos Peixes/virologia , Infecções por Parvoviridae/virologia , Parvovirus/patogenicidade , Tilápia/virologia , Animais , China , Efeito Citopatogênico Viral/efeitos dos fármacos , Baço/virologia
19.
Sci Rep ; 10(1): 12800, 2020 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-32733035

RESUMO

Ducks can shed and disseminate viruses and thus play a role in cross-species transmission. In the current study, we detected and characterised various avian parvoviruses and picornaviruses from wild Pacific black ducks, Chestnut teals, Grey teals and Wood ducks sampled at multiple time points from a single location using metagenomics. We characterised 46 different avian parvoviruses belonging to three different genera Dependoparvovirus, Aveparvovirus and Chaphamaparvovirus, and 11 different avian picornaviruses tentatively belonging to four different genera Sicinivirus, Anativirus, Megrivirus and Aalivirus. Most of these viruses were genetically different from other currently known viruses from the NCBI dataset. The study showed that the abundance and number of avian picornaviruses and parvoviruses varied considerably throughout the year, with the high number of virus reads in some of the duck samples highly suggestive of an active infection at the time of sampling. The detection and characterisation of several parvoviruses and picornaviruses from the individual duck samples also suggests co-infection, which may lead to the emergence of novel viruses through possible recombination. Therefore, as new and emerging diseases evolve, it is relevant to explore and monitor potential animal reservoirs in their natural habitat.


Assuntos
Animais Selvagens/virologia , Coinfecção/veterinária , Patos/virologia , Ecossistema , Genoma Viral/genética , Metagenômica , Infecções por Parvoviridae/veterinária , Parvovirus/genética , Infecções por Picornaviridae/veterinária , Picornaviridae/genética , Doenças das Aves Domésticas/virologia , Animais , Austrália , Coinfecção/virologia , Infecções por Parvoviridae/virologia , Parvovirus/isolamento & purificação , Picornaviridae/isolamento & purificação , Infecções por Picornaviridae/virologia
20.
Viruses ; 12(8)2020 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-32718049

RESUMO

An emaciated subadult free-ranging California sea lion (Csl or Zalophus californianus) died following stranding with lesions similar to 11 other stranded animals characterized by chronic disseminated granulomatous inflammation with necrotizing steatitis and vasculitis, involving visceral adipose tissues in the thoracic and peritoneal cavities. Histologically, affected tissues had extensive accumulations of macrophages with perivascular lymphocytes, plasma cells, and fewer neutrophils. Using viral metagenomics on a mesenteric lymph node six mammalian viruses were identified consisting of novel parvovirus, polyomavirus, rotavirus, anellovirus, and previously described Csl adenovirus 1 and Csl bocavirus 4. The causal or contributory role of these viruses to the gross and histologic lesions of this sea lion remains to be determined.


Assuntos
Linfonodos/patologia , Linfonodos/virologia , Leões-Marinhos/virologia , Serosite/patologia , Serosite/veterinária , Esteatite/patologia , Viroma , Anelloviridae/classificação , Anelloviridae/isolamento & purificação , Animais , Animais Selvagens , California , Feminino , Inflamação , Metagenômica , Parvovirus/classificação , Parvovirus/isolamento & purificação , Polyomavirus/classificação , Polyomavirus/isolamento & purificação , Serosite/virologia , Esteatite/virologia
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