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1.
Molecules ; 27(6)2022 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-35335210

RESUMO

Peperomia pellucida is a species known in the Amazon as "erva-de-jabuti" that has been used in several therapeutic applications based on folk medicine. Herein, we describe the classes, subclasses, and the main compounds of the leaves, stems, and roots from P. pellucida by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry associated with molecular networks, mirror plot on the GNPS library, and machine learning. These data show compounds that were annotated for the first time in the Peperomia genus, such as 2',4',5'-trihydroxybutyrophenonevelutin, dehydroretrofractamide C, and retrofractamide B.


Assuntos
Peperomia , Cromatografia Líquida de Alta Pressão/métodos , Medicina Tradicional , Peperomia/química , Folhas de Planta/química , Raízes de Plantas/química
2.
PLoS One ; 17(1): e0262794, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35061852

RESUMO

Peperomia pellucida L. Kunth is a herb well-known for its secondary metabolites (SM) with biological potential. In this study, the variations in the SM of P. pellucida during association with rhizobacteria were evaluated. Plants were inoculated with Enterobacter asburiae and Klebsiella variicola, which were identified by sequencing of the 16S rRNA gene. The data were evaluated at 7, 21, and 30-day post inoculation (dpi). Plant-bacteria symbiosis improved plant growth and weight. Total phenolic content and phenylalanine ammonia lyase enzyme activity had a significant increase mainly at 30 dpi. P. pellucida was mainly composed of phenylpropanoids (37.30-52.28%) and sesquiterpene hydrocarbons (39.28-49.42%). The phenylpropanoid derivative 2,4,5-trimethoxy-styrene (ArC2), the sesquiterpene hydrocarbon ishwarane, and the phenylpropanoid dillapiole were the major compounds. Principal component analysis (PCA) of the classes and compounds ≥ 2.0% indicated that plants colonized by E. asburiae had a reduction in the content of sesquiterpene hydrocarbons and an increase in phenylpropanoids and derivatives. Plants treated with this bacterium also had an increase in the content of 2,4,5-trimethoxystyrene at 30 dpi. Plants inoculated with K. variicola had significant increases only in the content of the classes monoterpene hydrocarbons and 'other compounds' (hydrocarbons, esters, ketones, etc.). These data suggest that the production of plant secondary metabolites can be modified depending on the type of rhizobacteria inoculated.


Assuntos
Peperomia/crescimento & desenvolvimento , Enterobacter/genética , Klebsiella/genética , Peperomia/metabolismo , Peperomia/microbiologia , Fenóis/metabolismo , Fenilalanina Amônia-Liase/metabolismo , Filogenia , Análise de Componente Principal , RNA Ribossômico 16S/genética , Rizosfera , Metabolismo Secundário , Compostos Orgânicos Voláteis/metabolismo
3.
J Nat Med ; 76(1): 259-267, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34529189

RESUMO

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and in Central America, it is considered one of the four most infectious diseases. This study aimed to screen the anti-trypanosomal activity of plant species from Salvadoran flora. Plants were selected through literature search for plants ethnobotanically used for antiparasitic and Chagas disease symptomatology, and reported in Museo de Historia Natural de El Salvador (MUHNES) database. T. cruzi was incubated for 72 h with 2 different concentrations of methanolic extracts of 38 species, among which four species, Piper jacquemontianum, Piper lacunosum, Trichilia havanensis, and Peperomia pseudopereskiifolia, showed the activity (≤ 52.0% viability) at 100 µg/mL. Separation of the methanolic extract of aerial parts from Piper jacquemontianum afforded a new flavanone (4) and four known compounds, 2,2-dimethyl-6-carboxymethoxychroman-4-one (1), 2,2-dimethyl-6-carboxychroman-4-one (2), cardamomin (3), and pinocembrin (5), among which cardamomin exhibited the highest anti-trypanosomal activity (IC50 = 66 µM). Detailed analyses of the spectral data revealed that the new compound 4, named as jaqueflavanone A, was a derivative of pinocembrin having a prenylated benzoate moiety at the 8-position of the A ring.


Assuntos
Extratos Vegetais/farmacologia , Tripanossomicidas , Trypanosoma cruzi , Doença de Chagas/tratamento farmacológico , Humanos , Meliaceae/química , Peperomia/química , Piper/química , Tripanossomicidas/farmacologia , Trypanosoma cruzi/efeitos dos fármacos
4.
Molecules ; 26(23)2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34885940

RESUMO

Peperomia Ruiz and Pav, the second largest genus of the Piperaceae, has over the years shown potential biological activities. In this sense, the present work aimed to carry out a seasonal and circadian study on the chemical composition of Peperomia circinata essential oils and aromas, as well as to evaluate the preliminary toxicity in Artemia salina Leach and carry out an in silico study on the interaction mechanism. The chemical composition was characterized by gas chromatography (GC/MS and GC-FID). In the seasonal study the essential oil yields had a variation of 1.2-7.9%, and in the circadian study the variation was 1.5-5.6%. The major compounds in the seasonal study were ß-phellandrene and elemicin, in the circadian they were ß-phellandrene and myrcene, and the aroma was characterized by the presence of ß-phellandrene. The multivariate analysis showed that the period and time of collection influenced the essential oil and aroma chemical composition. The highest toxicity value was observed for the essential oil obtained from the dry material, collected in July with a value of 14.45 ± 0.25 µg·mL-1, the in silico study showed that the major compounds may be related to potential biological activity demonstrated by the present study.


Assuntos
Artemia/efeitos dos fármacos , Óleos Voláteis/análise , Óleos Voláteis/toxicidade , Peperomia/química , Monoterpenos Acíclicos/análise , Monoterpenos Acíclicos/toxicidade , Alcenos/análise , Alcenos/toxicidade , Animais , Monoterpenos Cicloexânicos/análise , Monoterpenos Cicloexânicos/toxicidade , Pirogalol/análogos & derivados , Pirogalol/análise , Pirogalol/toxicidade , Estações do Ano
5.
Mol Phylogenet Evol ; 163: 107229, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34129936

RESUMO

Biological radiations provide unique opportunities to understand the evolution of biodiversity. One such radiation is the pepper plant family Piperaceae, an early-diverging and mega-diverse lineage that could serve as a model to study the diversification of angiosperms. However, traditional genetic markers lack sufficient variation for such studies, and testing hypotheses on poorly resolved phylogenetic frameworks becomes challenging. Limited genomic data is available for Piperaceae, which contains two of the largest genera of angiosperms, Piper (>2100 species) and Peperomia (>1300 species). To address this gap, we used genome skimming to assemble and annotate whole plastomes (152-161kbp) and >5kbp nuclear ribosomal DNA region from representatives of Piper and Peperomia. We conducted phylogenetic and comparative genomic analyses to study plastome evolution and investigate the role of hybridization in this group. Plastome phylogenetic trees were well resolved and highly supported, with a hard incongruence observed between plastome and nuclear phylogenetic trees suggesting hybridization in Piper. While all plastomes of Piper and Peperomia had the same gene content and order, there were informative structural differences between them. First, ycf1 was more variable and longer in Piper than Peperomia, extending well into the small single copy region by thousands of base pairs. We also discovered previously unknown structural variation in 14 out of 25 Piper taxa, tandem duplication of the trnH-GUG gene resulting in an expanded large single copy region. Other early-diverging angiosperms have a duplicated trnH-GUG, but the specific rearrangement we found is unique to Piper and serves to refine knowledge of relationships among early-diverging angiosperms. Our study demonstrates that genome skimming is an efficient approach to produce plastome assemblies for comparative genomics and robust phylogenies of species-rich plant genera.


Assuntos
Magnoliopsida , Peperomia , Piper , Evolução Molecular , Genômica , Magnoliopsida/genética , Peperomia/genética , Filogenia , Piper/genética
6.
Chemosphere ; 279: 130810, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34134431

RESUMO

To improve the remediation efficiency of plants on low concentration uranium-bearing wastewater and clarify its strengthening mechanism, Syngonium podophyllum-Peperomia tetraphylla co-planting system was established, the enhanced effects of plants interaction on uranium removal were investigated, the chemical forms, valence states, and subcellular distribution of uranium in plants were confirmed, and the mechanisms of alleviating uranium stress by plants interaction were revealed. In Syngonium podophyllum-Peperomia tetraphylla co-planting system, the total amount of ethanol-extracted uranium and deionized water-extracted uranium with higher toxicity in their roots were reduced by 10.30% and 7.17%, respectively, which reduced the toxicity of uranium to plants. Plants interaction can inhibit the reduction of U(VI) in the root of Peperomia tetraphylla, which is conducive to the transport of uranium from roots to shoots. In addition, uranium in plants mainly existed in the cell wall (54.44%-66.52%) and the soluble fraction (23.85%-32.89%). These results indicated that Syngonium podophyllum and Peperomia tetraphylla co-planting can enhance their effects of uranium removal by alleviating uranium stress with the cell wall immobilization and vacuole compartmentation, improving biomass of plants, increasing bioaccumulation factor and translocation factor of uranium.


Assuntos
Peperomia , Podophyllum , Urânio , Biodegradação Ambiental , Urânio/análise , Águas Residuárias
7.
World J Microbiol Biotechnol ; 37(1): 14, 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33394165

RESUMO

Chickpea (Cicer arietinum L., Fabaceae) is the second most important legume after common bean (Phaseolus vulgaris L., Fabaceae) and third in production among the legumes grains worldwide. Ascochyta blight and Fusarium wilt are among the main fungal infections which cause the major losses of chickpea crop. In this work we report the phyto-pathogen controlling properties of 24 endophyte Phomopsis/Diaporthe isolates on the chickpea fungal pathogens Ascochyta rabiei, Fusarium oxysporum and Fusarium solani. The Phomopsis/Diaporthe strains were isolated amongst a total of 62 endophytic fungi from the aerial parts of the herbaceous perennial American plant Peperomia obtusifolia (Piperaceae) along with Fusarium, Septoria, Colletotrichum, Alternaria and Roussoella genera among others. Phomopsis/Diaporthe isolates were identified as Diaporthe infecunda (12 isolates), Diaporthe sackstoni (1 isolate), Diaporthe cf. brasiliensis (4 isolates) and Phomopsis cf. tuberivora (7 isolates). All the Phomopsis/Diaporthe strains antagonized A. rabiei strain AR2 with a mean of inhibition (% I) of 86.59 ± 1.49% in dual cultures. The metabolic characterization of the Phomopsis/Diaporthe strains showed groups in three clusters which were in agreement with the taxonomic identification. Bioautographic evaluation of organic extracts showed that those of D. cf. brasiliensis and D. infecunda were better as inhibitors. Strain Po 45 was one of the most active (cluster 1, 96.87% I), and its ethyl acetate extract inhibited A. rabiei growth in a bioautographic assay until at least 10 µg/mm applied showing a specific chromatographic band as the responsible of the A. rabiei inhibition.


Assuntos
Ascomicetos/crescimento & desenvolvimento , Cicer/crescimento & desenvolvimento , Endófitos/fisiologia , Peperomia/microbiologia , Doenças das Plantas/prevenção & controle , Ascomicetos/classificação , Ascomicetos/isolamento & purificação , Ascomicetos/patogenicidade , Ascomicetos/fisiologia , Cicer/microbiologia , Técnicas de Cocultura , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/microbiologia , Endófitos/classificação , Endófitos/isolamento & purificação , Viabilidade Microbiana , Filogenia , Componentes Aéreos da Planta/microbiologia , Doenças das Plantas/microbiologia
8.
Nat Prod Res ; 35(10): 1657-1661, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-31140298

RESUMO

Aerial parts (leaves, flowers, stem) of Peperomia galioides extract administered to mice, was used to confirm its anti-inflammatory and sedative folk uses. The anti-inflammatory activity was assessed by croton oil-induced ear oedema and myeloperoxidase (acute inflammation); cotton pellet-induced granuloma (sub-acute inflammation) and Escherichia coli Lipopolysaccharide (LPS) induced inflammation (cellular mediators). The sedative activity was studied by the pentobarbital-induced sleeping time test. Single doses (300 and 600 mg/kg; i.p.) of the extract reduced croton oil-induced ear oedema and myeloperoxidase activity. Six days administration of the extract (300 mg/kg, i.p.) to mice implanted with cotton pellets diminished granuloma formation. LPS (20 mg/kg, i.p.) enhanced plasma nitrites and TNF-α levels that were inhibited by the extract. The duration but not the onset of sleeping time was enhanced by 300 and 600 mg/kg of the extract. Our results show that P. galioides has anti-inflammatory and sedative activities in mice, which validates its traditional use.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Hipnóticos e Sedativos/farmacologia , Peperomia/química , Extratos Vegetais/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Óleo de Cróton/toxicidade , Edema/induzido quimicamente , Edema/tratamento farmacológico , Hipnóticos e Sedativos/química , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Masculino , Camundongos , Peroxidase/metabolismo , Extratos Vegetais/química , Folhas de Planta/química , Plantas Medicinais/química , Sono/efeitos dos fármacos , Fator de Necrose Tumoral alfa/sangue
9.
Nat Prod Res ; 35(10): 1706-1710, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-31198050

RESUMO

Peperomia obtusifolia is a herbaceous perennial plant native to the Americas reported as a traditional medicine to treat snake bites and as a skin cleanser. The bioassay-guided fractionation of crude extracts from aerial parts of P. obtusifolia against a panel of clinically important fungi and bacteria, showed that hexane and dichloromethane extracts demonstrated selective bacterial inhibition, allowing the isolation of the known compounds peperobtusin A (1), and 3,4-dihydro-5-hydroxy-2,7-dimethyl-8-(3"-methyl-2"-butenyl)-2-(4'-methyl-1',3'-pentadienyl)-2H-1-benzopyran-6-carboxylic acid (2) from dichloromethane extract. Compound 2 was active against Gram-positive bacteria including community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates and an Enterococcus faecalis vancomycin-resistant strain, with minimal inhibitory concentration (MIC) values of 4 µg/mL (10.8 µM) and 8 µg/mL (21.6 µM) respectively. The interaction of compound 2 with the bacterial membrane was demonstrated by means of Zeta potential experiments on S. aureus, then confirming the membrane damage by fluorescent microscopy experiments.


Assuntos
Antibacterianos/farmacologia , Benzopiranos/farmacologia , Peperomia/química , Prenilação , Lipossomos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Eletricidade Estática
10.
J Asian Nat Prod Res ; 23(9): 851-858, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33118386

RESUMO

Two isopentenyl resorcinols, peperobtusin B and peperobtusin C, have been isolated from Peperomia tetraphylla. Their structures were determined on the basis of spectroscopic methods, especially 1H NMR, 13C NMR, 2D NMR, and HR-TOF-MS. Two compounds were evaluated for cytostatic activity against G2, A 549, Hela and HCT 116 cells, but cytostatic activity of both compounds is weak.


Assuntos
Peperomia , Células HeLa , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Resorcinóis/farmacologia
11.
Food Chem ; 344: 128738, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33280962

RESUMO

This study aimed to compare the effect of fermentation and drying on the organoleptic characteristic, total phenolic content, antioxidant and anti-inflammatory activities of Peperomia pellucida (L.) Kunth tea with commercial Camellia sinensis tea. The phenolic content, antioxidant and anti-inflammatory activities in P. pellucida were significantly (p < 0.05) lower than C. sinensis, irrespective of the fermentation and drying methods. Although fermentation decreased the total phenolics, flavonoids and antioxidant activity in both P. pellucida and C. sinensis teas, the anti-inflammatory potential of P. pellucida was significantly (p < 0.05) improved. Principle component analysis revealed that fermentation and drying methods contributed to respective 42.3% and 27.2% of activity variation in P. pellucida. The browning index was positively correlated with fermentation index (r = 0.670, p < 0.05) of leaves samples. Overall, unfermented and fermented P. pellucida leaves were best dried with microwaving and freeze drying, respectively for optimal antioxidant and anti-inflammatory activities with favorable consumer's acceptance.


Assuntos
Anti-Inflamatórios/análise , Antioxidantes/análise , Dessecação/métodos , Fermentação , Peperomia/química , Chás de Ervas/análise , Flavonoides/análise , Fenóis/análise , Folhas de Planta/química
12.
Daru ; 29(1): 147-158, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33813721

RESUMO

Spray-dried extracts are prepared as powders or granules after solvent removal, which can be obtained in the presence or absence of pharmaceutical adjuvants. This work aimed to optimize the process of obtaining dried extracts of Peperomia pellucida L. (HBK) by spray drying. The characterization of the extract was performed by thermal analysis, specific surface area, particle size and high performance liquid chromatography (HPLC); then, capsules were developed for antimicrobial treatment, evaluating four bench lots by the determination of the angle of repose and time of flow, scanning electron microscopy, porosity and physicochemical quality control. There were no significant differences between the extracts obtained by spray drying at atomization temperatures of 140 °C, 160 °C and 180 °C, which was confirmed by thermal analysis. Specific surface area varied inversely with the mean particle size. Regarding the marker content by HPLC, no significant differences were found between the samples, although the flavonoid fraction was more stable at 160 °C. Bench lots (I to IV) were developed using the diluents Flowlac®, Starch® 1500, microcrystalline cellulose 250 and Cellactose® 80. Based on the results, the bench lot I, containing Flowlac®, was selected. The results of physicochemical quality control demonstrated that the selected formulation meets the pre-established parameters, and proving to be economically viable.


Assuntos
Peperomia , Extratos Vegetais/química , Química Farmacêutica/métodos , Liberação Controlada de Fármacos , Tamanho da Partícula , Porosidade , Secagem por Atomização , Propriedades de Superfície , Temperatura
13.
Molecules ; 25(21)2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33114252

RESUMO

Extracts of Peperomia pellucida [L.] Kunth have previously been demonstrated to have in vivo estrogenic-like effects, thereby functioning as an anti-osteoporotic agent. However, the compounds responsible for these effects have not yet been determined. Therefore, the aim of this study is to isolate and elucidate potential compounds with estrogenic activity. The structures of the isolated compounds were identified using 1D 1H and 13C-NMR and confirmed by 2D FT-NMR. The estrogenic activity was evaluated using the E-SCREEN assay, and a molecular docking study was performed to predict the binding affinity of the isolated compounds to estrogen receptors. In this experiment, we successfully isolated three phenylpropanoids and two lignan derivatives, namely, 6-allyl-5-methoxy-1,3-benzodioxol-4-ol (1), pachypostaudin B (2), pellucidin A (3), dillapiole (4), and apiol (5). Among these compounds, the isolation of 1 and 2 from P. pellucida is reported for the first time in this study. Activity assays clearly showed that the ethyl acetate extract and its fractions, subfractions, and isolated compounds exerted estrogenic activity. Methanol fraction of the ethyl acetate extract produced the highest estrogenic activity, while 1 and 2 had partial agonist activity. Some compounds (derivates of dillapiole and pellucidin A) also had, in addition, anti-estrogenic activity. In the docking study, the estrogenic activities of 1-5 appeared to be mediated by a classical ligand-dependent mechanism as suggested by the binding interaction between the compounds and estrogen receptors; binding occurred on Arg 394 and His 524 of the alpha receptor and Arg 346 and His 475 of the beta receptor. In summary, we reveal that P. pellucida is a promising anti-osteoporotic agent due to its estrogenic activity, and the compounds responsible for this activity were found to be lignan and phenylpropanoid derivatives. The presence of other compounds in either the extract or fraction may contribute to a synergistic effect, as suggested by the higher estrogenic activity of the methanol fraction. Hence, we suggest further research on the osteoporotic activity and safety of the identified compounds, especially regarding their effects on estrogen-responsive organs.


Assuntos
Lignanas/isolamento & purificação , Lignanas/farmacologia , Peperomia/química , Fitoestrógenos/isolamento & purificação , Fitoestrógenos/farmacologia , Propanóis/isolamento & purificação , Propanóis/farmacologia , Receptor alfa de Estrogênio/metabolismo , Receptor beta de Estrogênio/metabolismo , Humanos , Lignanas/metabolismo , Células MCF-7 , Modelos Moleculares , Simulação de Acoplamento Molecular , Fitoestrógenos/metabolismo , Propanóis/química
14.
PLoS One ; 15(9): e0238834, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32941458

RESUMO

Peperomia pellucida (PP) belongs to the Peperomia genus, which has a pantropic distribution. PP is used to treat a wide range of symptoms and diseases, such as pain, inflammation, and hypertension. Intriguingly, PP extract is used by different tropical countries for its anti-inflammatory and antinociceptive effects. In fact, these outcomes have been shown in animal models, though the exact bioactive products of PP that exert such results are yet to be discovered. To determine and elucidate the mechanism of action of one of these compounds, we evaluated the antinociceptive effect of the novel dimeric ArC2 compound, Pellucidin A by using in vivo and in silico models. Animals were then subjected to chemical, biphasic and thermal models of pain. Pellucidin A induced an antinociceptive effect against chemical-induced pain in mice, demonstrated by the decrease of the number of writhes, reaching a reduction of 43% and 65% in animals treated with 1 and 5 mg/kg of Pellucidin A, respectively. In the biphasic response (central and peripheral), animals treated with Pellucidin A showed a significant reduction of the licking time exclusively during the second phase (inflammatory phase). In the hot-plate test, Pellucidin A did not have any impact on the latency time of the treated animals. Moreover, in vivo and in silico results show that Pellucidin A's mechanism of action in the inflammatory pain occurs most likely through interaction with the nitric oxide (NO) pathway. Our results demonstrate that the antinociceptive activities of Pellucidin A operate under mechanism(s) of peripheral action, involving inflammatory mediators. This work provides insightful novel evidence of the biological properties of Pellucidin A, and leads to a better understanding of its mechanism of action, pointing to potential pharmacological use.


Assuntos
Analgésicos/farmacologia , Ciclobutanos/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Ciclo-Oxigenase 2/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Inflamação/tratamento farmacológico , Camundongos , Simulação de Acoplamento Molecular , Óxido Nítrico/metabolismo , Dor/tratamento farmacológico , Dor/fisiopatologia , Medição da Dor , Peperomia , Extratos Vegetais/farmacologia
15.
J Chem Inf Model ; 59(5): 1858-1872, 2019 05 28.
Artigo em Inglês | MEDLINE | ID: mdl-31117526

RESUMO

Bioassay-guided isolation protocol was performed on petroleum ether extract of Peperomia blanda (Jacq.) Kunth using column chromatographic techniques. Five compounds were isolated and their structures were elucidated via one-dimensional (1D) and two-dimensional (2D) NMR, gas chromatography mass sectroscopy (GCMS), liquid chromatography mass spectroscopy (LCMS), and ultraviolet (UV) and infrared (IR) analyses. Dindygulerione E (a new compound), and two compounds isolated from P. blanda for the first time-namely, dindygulerione A and flavokawain A-are reported herein. Antimicrobial activity was screened against selected pathogenic microbes, and minimum inhibitory concentrations (MIC) were recorded within the range of 62-250 µg/mL. Assessment of the pharmacotherapeutic potential has also been done for the isolated compounds, using the Prediction of Activity spectra for Substances (PASS) software, and different activities of compounds were predicted. Molecular docking, molecular dynamics simulation and molecular mechanics/Poisson-Boltzmann Surface Area (MM-PBSA) calculations have proposed the binding affinity of these compounds toward methylthioadenosine phosphorylase enzyme, which may explain their inhibitory actions.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Peperomia/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Antibacterianos/isolamento & purificação , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Humanos , Testes de Sensibilidade Microbiana , Modelos Moleculares , Petróleo/análise , Extratos Vegetais/isolamento & purificação
16.
J Ethnopharmacol ; 232: 90-102, 2019 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-30562552

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Peperomia pellucida (L.) Kunth is an annual weed with a preference to humid places with reduced solar radiation. This plant is mainly distributed in the Neotropics, Africa, Southeast Asia, and Australia. It is popularly employed in the treatment of a variety of health conditions such as abscesses, abdominal pain, skin sores, conjunctivitis, measles, and kidney troubles. Several studies have also described its antimicrobial, cytotoxic, antidiabetic and a variety of other bioactivities. THE AIM OF THE REVIEW: The aim of this work is to evaluate, using a critical review, the present ethnomedicinal applications, phytochemistry and pharmacological studies of P. pellucida essential oils (EOs) and extracts from different locations around the world. MATERIALS AND METHODS: This review was performed through an online survey of the ethnomedicinal practices, chemical compositions and pharmacological applications of P. pellucida EOs and extracts. The data were mainly obtained from online journals and books published in English, Portuguese and Spanish. The information was collected from websites such as Google, Google Scholar, PubMed, Science Direct, ResearchGate and other online databases that provided more information about this herb. RESULTS: Peperomia pellucida bioactivities such as antimicrobial, cytotoxic, antioxidant, fracture healing, antidiabetic and anti-hypercholesterolemia have been described in several literature sources. Nonetheless, most reports only provide the phytochemical screening of extracts, which does not allow the identification of the active compounds. From these studies, some reported constituents are not included in the Dictionary of Natural Products (DNP), which raises questions toward their identification. In addition, some biological assays were even performed without standard controls for comparison which also makes these results questionable. CONCLUSION: This review evaluates data regarding the phytopharmaceutical potential of P. pellucida. In general, several important aspects were questionable or missing in these manuscripts, which points out the need of more investigation on the pharmacological properties and phytochemical compositions of this herb.


Assuntos
Peperomia , Compostos Fitoquímicos/farmacologia , Fitoterapia , Animais , Humanos , Medicina Tradicional , Peperomia/química , Compostos Fitoquímicos/análise
17.
Bol. latinoam. Caribe plantas med. aromát ; 17(6): 610-618, nov. 2018. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1007367

RESUMO

Peperomia hispidula (Sw.) A. Dietr. is used in Mexican traditional medicine for treating respiratory illnesses such as asthma. The latter disorder results from an excessive and inappropriate constriction of airway smooth muscle. The aim of the present study was to evaluate the relaxant activity of P. hispidula on isolated rat tracheal rings contracted with carbachol. The methyleugenol was identified as the main active constituent in the dichloromethane extract. To explore the possible mechanism of action, concentration-response curves were constructed in the presence and absence of propranolol (3 µM), indomethacin (10 µM), glibenclamide (1 µM), and L-NAME (300 µM), finding that neither reduced methyleugenol-induced smooth muscle relaxation. In conclusion, P. hispidula herein displayed relaxant activity on rat tracheal rings. The effect of methyleugenol, was probably not related to the activation of ß2-adrenoceptors, prostaglandins, K+ATP channels or nitric oxide.


Peperomia hispidula (Sw.) A. Dietr. es utilizada en la medicina tradicional mexicana para tratar enfermedades respiratorias como el asma. Este último trastorno es el resultado de una contracción excesiva e inapropiada del músculo liso de las vías respiratorias. El objetivo del presente estudio fue evaluar la actividad relajante de P. hispidula sobre anillos aislados de tráquea de rata contraídos con carbacol. El metileugenol fue identificado como el principal constituyente activo en el extracto de diclorometano. Para explorar el posible mecanismo de acción, se construyeron curvas concentración-respuesta en presencia y ausencia de propranolol (3 µM), indometacina (10 µM), glibenclamida (1 µM), y L-NAME (300 µM), encontrando que ninguno redujo la relajación del músculo liso inducida por metileugenol. En conclusión, P. hispidula muestra actividad relajante en anillos de tráquea de rata. El efecto de metileugenol, al parecer no está implicado con la activación de los receptores ß2-adrenérgicos, prostaglandinas, canales de K+ATP u óxido nítrico.


Assuntos
Animais , Masculino , Ratos , Traqueia/efeitos dos fármacos , Eugenol/análogos & derivados , Eugenol/farmacologia , Extratos Vegetais/farmacologia , Peperomia , Asma/metabolismo , Estenose Traqueal/induzido quimicamente , Eugenol/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem , Cloreto de Metileno/química , Relaxamento Muscular/efeitos dos fármacos
18.
PLoS One ; 13(6): e0198682, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29924840

RESUMO

In this study, we aimed to evaluate the immunomodulatory effects of crude leaf extracts from Piper gaudichaudianum Kunth, P. arboreum Aub., P. umbellata L., P. fuligineum Kunth, and Peperomia obtusifolia A. Dietr. on an in vitro model of inflammatory response. The crude extracts were previously obtained by maceration of the leaves. The half-maximal inhibitory concentration was determined by the MTT assay using human peripheral blood mononuclear cells. Human monocytes were simultaneously challenged with each crude extract and lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, to induce a strong inflammatory response. After 24 h of incubation, cell-free supernatants were used for evaluating the mediators involved in inflammation: H2O2, TNF-α, IL-8, IL-6, IL-1ß, IL-10, IL-12, FGF-b, and TGF-ß1. We also compared the results with the effects of ketoprofen, a well-known anti-inflammatory drug. The P. gaudichaudianum crude extract downmodulated the production of H2O2, IL-1ß, IL-6, IL-8, and TGF-ß1 by LPS-stimulated monocytes; P. arboreum, IL-1ß, IL-6, IL-8, and TNF-α; P. umbellata and P. fuligineum, H2O2, IL-1ß, IL-6, IL-8, IL-10, and TNF-α; and P. obtusifolia, H2O2, IL-6, IL-8, IL-10, and TNF-α. In general, the crude leaf extracts amplified the anti-inflammatory response when compared with ketoprofen, particularly reducing the production of IL-8, a mediator involved in neutrophil recruitment during tissue damage. Thus, the crude leaf extracts of P. gaudichaudianum, P. arboreum, P. umbellata, P. fuligineum, and Peperomia obtusifolia elicited an anti-inflammatory response against LPS-challenged monocytes. These findings show the anti-inflammatory properties of these crude leaf extracts and offer new perspectives for their use in the treatment of inflammatory diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Monócitos/efeitos dos fármacos , Peperomia/química , Piper/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Acetatos , Anti-Inflamatórios/isolamento & purificação , Brasil , Células Cultivadas , Clorofórmio , Citocinas/metabolismo , Avaliação Pré-Clínica de Medicamentos , Etanol , Hexanos , Humanos , Concentração Inibidora 50 , Cetoprofeno/farmacologia , Lipopolissacarídeos/farmacologia , Monócitos/imunologia , Monócitos/metabolismo , Extratos Vegetais/isolamento & purificação , Especificidade da Espécie
19.
Rev. peru. med. integr ; 3(1): 9-17, 2018. tab, graf
Artigo em Espanhol | MOSAICO - Saúde integrativa, LILACS, MOSAICO - Saúde integrativa | ID: biblio-1145619

RESUMO

Objetivo. Evaluar la toxicidad aguda de los extractos etanólicos del Eucalyptus globulus Labill. (eucalipto), Morinda citrifolia L. (noni), Peperomia glauca (pino) (congona), Schinus molle L. (molle) y Zea mays L. (Maíz morado) en ratones Balb/c 53. Materiales y métodos. Se utilizaron 60 ratones machos divididos en seis grupos (eucalipto, noni, congona, molle, maíz morado y control). Los grupos tratados recibieron por vía oral una dosis única de 2000 mg/kg de los extractos etanólicos, y el grupo control recibió polisorbato 2 mL/kg al 3%. Se evaluó ganancia de peso, valores hematológicos (hematocrito, eritrocitos, hemograma, leucocitos, plaquetas), bioquímica sérica (úrea, creatinina, ALT, proteínas totales, albumina, globulinas), histopatología hepática y renal. Resultados. Se observó signos de inquietud, excitación y aparente fotosensibilidad en el grupo eucalipto, por una hora. Se encontró leucopenia en grupos congona, molle, eucalipto y maíz morado; trombocitopenia en grupos eucalipto y molle, y elevación del ALT en los grupos congona y eucalipto, en comparación con los valores del grupo control. La ganancia de peso, los demás valores hematológicos, así como la bioquímica renal y hepática en los otros grupos no fueron significativos. Conclusiones. En las condiciones experimentales no se observó signos de toxicidad ni mortalidad en el ensayo; la DL50 de los extractos etanólicos estaría sobre los 2000 mg/kg.


Objective. This study was performed to evaluate the acute toxicity of ethanolic extracts of Eucalyptus globulus Labill. (Eucalipto), Morinda citrifolia L. (noni), Peperomia glauca (pino) (Congona), Schinus molle L. (molle) y Zea mays L. (maíz morado) in Balb/c mice. Materials and methods. Sixty male mice were divided into 6 groups (Eucalipto, Noni, Congona, Molle, Maíz morado and Control) of 10 each were used. The guide for the Organization for Economic Cooperation and Development (OECD 423) was followed for the study. The treated group received for gavage a single dose at 2000 mg/kg and the control group received polysorbate at 2 mL/kg at 3%. Weight gain, hematological values (hematocrit, erythrocytes, hemogram, leukocytes, platelets), serum biochemistry (urea, creatinine, ALT, total proteins, albumin, globulins), hepatic and renal histopathology were performed. Results. No signs of mortality and morbidity were observed as a consequence of the administration of the extracts, except for the Eucalipto group, which presented restlessness, excitation and apparent photosensitivity for one hour. There was leukopenia in Congona, Molle, Eucalipto and Maíz morado groups; thrombocytopenia in groups Eucalipto and Molle; and elevations of ALT in Congona and Eucalipto groups in comparison than the values from control group. Weight gain and other hematological values, as well as renal and hepatic biochemistry in the other groups were not significant. Conclusions. Under experimental conditions no signs of toxicity or mortality were observed in the trial; the LD50 of the ethanolic extracts would be above 2000 mg/kg.


Assuntos
Schinus molle/toxicidade , Zea mays/toxicidade , Peperomia/toxicidade , Morinda/toxicidade , Eucalyptus/toxicidade , Plantas Medicinais , Extratos Vegetais , Testes de Toxicidade , Medicina Tradicional , Camundongos Endogâmicos BALB C
20.
Fitoterapia ; 122: 80-84, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28859930

RESUMO

Nineteen secolignans (1-19), including five new ones (1-5), were isolated from the whole plant of Peperomia dindygulensis. Their structures including stereochemistry were determined by spectroscopic methods, in particular NMR and electronic CD (ECD) analysis. All the isolates were evaluated for their inhibitory activities against IFN-γ/STAT1 as well as IL-6/STAT3 signaling pathway by the method of Luciferase assay. Six 2-methene type secolignans (1, 2, 6-9) exhibited significant inhibitory activities against JAK-STAT pathways with the IC50 values both lower than 10µM.


Assuntos
Lignanas/farmacologia , Peperomia/química , Transdução de Sinais/efeitos dos fármacos , Células Hep G2 , Humanos , Interleucina-6/metabolismo , Estrutura Molecular , Fator de Transcrição STAT1/metabolismo , Fator de Transcrição STAT3/metabolismo
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