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1.
J Cutan Pathol ; 49(3): 215-219, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34427943

RESUMO

BACKGROUND: Within the field of pathology there is a need for a uniform low-cost option for securing high-quality photomicrographs. Advances in smartphone photography and 3D-printing technology allow for custom adapters to be designed for the purpose of photomicrograph capture. METHODS: Photomicrograph acquisition was performed using four core modalities: a novel 3D-printed smartphone-to-microscope adapter, freehand smartphone-to-microscope technique, a commercial adaptor (LabCam Pro), and a microscope-mounted digital camera. Eight skin diagnoses were photographed using each of the modalities and time to image capture was measured. The photomicrographs were blindly reviewed by two academic dermatopathologists and one pathologist using a side-by-side comparison technique to determine the image quality. Cost assessments were evaluated by obtaining free pricing information on manufacturer websites. RESULTS: The 3D-printed adapter was the most efficient method of capturing a high-quality photomicrograph in addition to being budget neutral. The microscope-mounted camera produced the highest quality photomicrographs followed by the 3D-printed adapter. CONCLUSIONS: The 3D-printed smartphone-to-microscope adapter offers a low-cost, time-efficient method of capturing high-quality photomicrographs.


Assuntos
Fotomicrografia , Smartphone , Custos e Análise de Custo , Humanos , Patologia Clínica , Fotomicrografia/instrumentação , Impressão Tridimensional , Dermatopatias/patologia
2.
Neuroimage ; 248: 118790, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-34933123

RESUMO

Abnormal tau inclusions are hallmarks of Alzheimer's disease and predictors of clinical decline. Several tau PET tracers are available for neurodegenerative disease research, opening avenues for molecular diagnosis in vivo. However, few have been approved for clinical use. Understanding the neurobiological basis of PET signal validation remains problematic because it requires a large-scale, voxel-to-voxel correlation between PET and (immuno) histological signals. Large dimensionality of whole human brains, tissue deformation impacting co-registration, and computing requirements to process terabytes of information preclude proper validation. We developed a computational pipeline to identify and segment particles of interest in billion-pixel digital pathology images to generate quantitative, 3D density maps. The proposed convolutional neural network for immunohistochemistry samples, IHCNet, is at the pipeline's core. We have successfully processed and immunostained over 500 slides from two whole human brains with three phospho-tau antibodies (AT100, AT8, and MC1), spanning several terabytes of images. Our artificial neural network estimated tau inclusion from brain images, which performs with ROC AUC of 0.87, 0.85, and 0.91 for AT100, AT8, and MC1, respectively. Introspection studies further assessed the ability of our trained model to learn tau-related features. We present an end-to-end pipeline to create terabytes-large 3D tau inclusion density maps co-registered to MRI as a means to facilitate validation of PET tracers.


Assuntos
Doença de Alzheimer/diagnóstico por imagem , Doença de Alzheimer/metabolismo , Aprendizado Profundo , Neuroimagem/métodos , Proteínas tau/metabolismo , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/metabolismo , Conjuntos de Dados como Assunto , Desenho de Equipamento , Feminino , Humanos , Imageamento Tridimensional , Imageamento por Ressonância Magnética , Masculino , Fotomicrografia/instrumentação , Tomografia Computadorizada por Raios X
3.
Rev. ADM ; 78(3): 135-141, mayo-jun. 2021. ilus, tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1254493

RESUMO

Se presentan los casos de fracturas verticales sintomáticos que ameritaron la extracción de las órganos dentales afectados en un periodo de cinco meses durante la pandemia por COVID-19 en la ciudad de Chihuahua, México. Material y métodos: Se recolectaron 26 muestras para el estudio, proporcionadas por los cirujanos dentistas integrantes del Grupo de Egresados de la Facultad de Odontología de la Universidad Autónoma de Chihuahua. Resultados: Los resultados muestran diferencias a lo reportado previamente respecto al tipo de diente involucrado, sexo de los pacientes, restauraciones coronales y tratamientos endodóncicos previos. Conclusiones: Se puede suponer que las modificaciones en el estilo de vida de la población, sumadas a la angustia permanente, pueden ser factores que contribuyen a las fracturas dentales (AU)


There are cases of symptomatic vertical fractures that warranted the extraction of affected teeth are presented, over a period of five months during the COVID-19 pandemic in Chihuahua City, Mexico. Material and methods: 26 samples were collected for the study, provided by the dentist who were members of the Graduate Group of the Faculty of Dentistry of the Autonomous University of Chihuahua. Results: The results show differences from what was previously reported regarding the type of tooth involved, sex of patients, coronal restorations and previous endodontic treatments. Conclusions: It can be assumed that changes in the lifestyle of the population, added to permanent distress, may be factors to preserve dental fractures (AU)


Assuntos
Fraturas dos Dentes/epidemiologia , Raiz Dentária/lesões , Infecções por Coronavirus , COVID-19 , Tratamento do Canal Radicular/estatística & dados numéricos , Faculdades de Odontologia , Dente Pré-Molar , Fissuras Dentárias/epidemiologia , Fotomicrografia , Interpretação Estatística de Dados , Técnica para Retentor Intrarradicular/estatística & dados numéricos , Coroa do Dente/lesões , Restauração Dentária Permanente/estatística & dados numéricos , Distribuição por Idade e Sexo , México/epidemiologia , Dente Molar
4.
Tex Heart Inst J ; 48(1)2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-33946109

RESUMO

Papillary fibroelastomas are benign tumors that usually originate from cardiac valves but may have other endocardial origins. We report the cases of 2 patients in whom left atrial appendage masses were initially diagnosed as thrombus. They were treated for embolic stroke and their symptoms resolved; however, their left atrial appendage masses did not regress. After surgery, histologic analysis of the resected masses revealed papillary fibroelastoma in both cases. We discuss the diagnostic and therapeutic dilemmas encountered in patients with papillary fibroelastomas and cardiac masses other than thrombus.


Assuntos
Fibroelastoma Papilar Cardíaco/diagnóstico , Neoplasias Cardíacas/diagnóstico , Idoso , Apêndice Atrial , Diagnóstico Diferencial , Ecocardiografia Transesofagiana , Humanos , Masculino , Músculos Papilares , Fotomicrografia
5.
Rev. Asoc. Odontol. Argent ; 109(1): 41-48, ene.-abr. 2021. ilus, tab
Artigo em Espanhol | LILACS | ID: biblio-1281207

RESUMO

Objetivo: En los granulomas periapicales, los plasmocitos (PL) participan activamente mediante la liberación de inmunoglobulinas. El propósito de este ensayo fue identificar y contar el número de PL en diferentes períodos de tiempo en lesiones periapicales experimentales en ratas. Materiales y métodos: Mediante la exposición al medio oral de la pulpa de los primeros molares inferiores izquierdos, se indujeron granulomas periapicales en ratas a las que previamente se les suministró anestesia. La pulpa de los primeros molares inferiores derechos no fue expuesta, y estos dientes se utilizaron como control. Los animales fueron eutanasiados a los 10, 30 y 60 días de la exposición. Los maxilares inferiores fueron removidos, y los primeros molares, junto con los tejidos circundantes, se procesaron para su estudio histológico. Se obtuvieron secciones semiseriadas, posteriormente coloreadas con verde de metilo-pironina (VMP). Cada tres secciones, las tres siguientes fueron coloreadas con hematoxilina y eosina (H-E). Los controles también fueron coloreados con H-E. Resultados: Todos los especímenes experimentales coloreados con H-E revelaron la presencia de granulomas periapicales. Luego de la exposición pulpar, el número de PL que reaccionó positivamente al VMP se incrementó de manera progresiva desde el día 10 hasta los días 30 y 60. A pesar de que a los 60 días el número de PL fue ligeramente menor que a los 30 días, no hubo diferencias estadísticamente significativas entre estos dos períodos. Los especímenes del grupo control coloreados con H-E mostraron que los tejidos periapicales se encontraban dentro de los parámetros normales en todos los períodos de observación. Conclusiones: Los resultados de este estudio revelaron que el número de plasmocitos VMP positivos se incrementa progresivamente en función del tiempo transcurrido pero se estabiliza al finalizar el experimento. También sugieren que el empleo de la coloración de VMP es un procedimiento adecuado para la identificación y la cuantificación de plasmocitos en los granulomas periapicales inducidos experimentalmente en ratas (AU)


Aim: Plasma cells (PL) release immunoglobulin in periapical lesions. The purpose of this assay was to identify and count the number of plasmocytes observed in periapical lesions in rats. Materials and methods: By exposing the pulp of the lower left first molars to the oral environment, periapical granulomas were induced in rats previously anesthetized. The pulp of right mandibular first molars was not exposed and these teeth were used as negative controls. The animals were euthanized at 10, 30 and 60 days after pup exposure. The mandibles were removed and specimens of the molar teeth along with the surrounding tissues were prepared for histology. Semi serial sections of the left first molar were stained with methyl green pyronine (MGP). Every three sections, the following three sections were stained with hematoxilyn and eosin (H-E). Negative control samples were stained with H-E. Results: All the H-E stained experimental samples revealed the presence of periapical granulomas. After pulp exposure, the number of PL increased from day 10 to 30 and 60. In the 60-day samples the number of PL was slightly less than that of the 30-day samples, with no statistically significant difference. The H-E stained control samples showed normal periapical tissues in all observation periods. Conclusions: The results of this study revealed that the number of VMP positive PL, increased progressively with time but it was stabilized at the end of the experiment. In addition, the results suggest that the use of VMP stain is a suitable procedure for the identification and counting of PL in experimentally induced periapical granulomas in rats (AU)


Assuntos
Animais , Ratos , Granuloma Periapical/imunologia , Plasmócitos/imunologia , Inflamação/fisiopatologia , Tecido Periapical , Imunoglobulinas , Fotomicrografia , Interpretação Estatística de Dados , Técnicas Histológicas , Radiografia Dentária Digital
6.
Ann Diagn Pathol ; 52: 151733, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33780691

RESUMO

Among four sub-patterns of Gleason grade 4 prostate cancer, voluminous evidence supports that the cribriform pattern holds an unfavorable prognostic impact, as compared with poorly-formed, fused, or glomeruloid. The International Society of Urological Pathology (ISUP) recommends specifying whether invasive grade 4 cancer is cribriform. Recently, ISUP experts published a consensus definition of cribriform pattern highlighting criteria that distinguish it from mimickers. The current study aimed to analyze morphologic features separately to identify those that define the essence of the cribriform pattern. Thirty-two selected photomicrographs were classified by 12 urologic pathologists as: definitely cribriform cancer, probably cribriform, unsure, probably not cribriform, or definitely not cribriform. Consensus was defined as 9/12 agree or disagree, with ≤1 strongly supporting the opposite choice. Final consensus was achieved in 21 of 32 cases. Generalized estimating equation (GEE) model with logit link was fitted to estimate effect of multiple morphologic predictors. Fisher exact test was used for categorical findings. Presence of intervening stroma precluded calling cribriform cancer (p = 0.006). Mucin presence detracted (p = 0.003) from willingness to call cribriform cancer (only 3 cases had mucin). Lumen number was associated with cribriform consensus (p = 0.0006), and all consensus cases had ≥9 lumens. Predominant papillary pattern or an irregular outer boundary detracted (p = NS). Invasive cribriform carcinoma should have absence of intervening stroma, and usually neither papillary pattern, irregular outer boundary, nor very few lumens. Setting the criteria for cribriform will help prevent over- or undercalling this important finding.


Assuntos
Adenocarcinoma/patologia , Gradação de Tumores/métodos , Invasividade Neoplásica/patologia , Neoplasias da Próstata/patologia , Adenocarcinoma/diagnóstico , Adenocarcinoma/metabolismo , Consenso , Humanos , Masculino , Mucinas/metabolismo , Patologistas/organização & administração , Patologistas/estatística & dados numéricos , Fotomicrografia/métodos , Fotomicrografia/estatística & dados numéricos , Prognóstico , Neoplasias da Próstata/classificação , Neoplasias da Próstata/diagnóstico , Neoplasias da Próstata/metabolismo , Sociedades Médicas/organização & administração , Inquéritos e Questionários/estatística & dados numéricos , Urologistas/organização & administração , Urologistas/estatística & dados numéricos
7.
J Cutan Pathol ; 48(1): 24-33, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33410541

RESUMO

BACKGROUND: Oral focal mucinosis (OFM) is a rare benign condition of unknown etiology, considered the oral counterpart of cutaneous focal mucinosis. We report the clinicopathologic features of 21 cases of OFM in conjunction with a review of the literature. METHODS: Clinical data were collected from the records of five oral and maxillofacial pathology services. All cases were evaluated by hematoxylin and eosin staining, histochemistry, and immunohistochemistry (vimentin, S-100, α-SMA, CD34, and mast cell). RESULTS: The series comprised 14 females (66.7%) and seven males (33.3%), with a mean age of 48.2 ± 20.7 years (range: 8-77 years) and a 2:1 female-to-male ratio. Most of the lesions affected the gingiva (n = 6, 28.6%) and presented clinically as asymptomatic sessile or pedunculated nodules with fibrous or hyperplasic appearance. All cases were negative for S-100 protein, CD34, and α-SMA and positive for Alcian blue staining. Conservative surgical excision was the treatment in all cases, and there was only one recurrence. CONCLUSION: OFM is a rare benign disorder that is often clinically misdiagnosed as reactive lesions or benign proliferative processes. Dermatologists and pathologists should consider OFM in the differential diagnosis of soft tissue lesions in the oral cavity, mainly located in the gingiva.


Assuntos
Boca/patologia , Mucinoses/diagnóstico , Mucinoses/cirurgia , Neoplasias de Tecidos Moles/patologia , Actinas/metabolismo , Adulto , Idoso , Azul Alciano , Antígenos CD34/metabolismo , Conscientização , Estudos de Casos e Controles , Dermatologistas , Diagnóstico Diferencial , Erros de Diagnóstico , Feminino , Humanos , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Mucinoses/etiologia , Mucinoses/metabolismo , Patologistas , Fotomicrografia/métodos , Recidiva , Proteínas S100/metabolismo , Coloração e Rotulagem/métodos
8.
J Neuroophthalmol ; 41(2): 194-199, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32141976

RESUMO

BACKGROUND: Compression of the optic chiasm typically leads to bitemporal hemianopia. This implies that decussating nasal fibers are selectively affected, but the precise mechanism is unclear. Stress on nasal fibers has been investigated using finite element modeling but requires accurate anatomical data to generate a meaningful output. The precise shape of the chiasm is unclear: A recent photomicrographic study suggested that nasal fibers decussate paracentrally and run parallel to each other in the central arm of an "H." This study aimed to determine the population variation in chiasmal shape to inform future models. METHODS: Sequential MRI scans of 68 healthy individuals were selected. 2D images of each chiasm were created and analyzed to determine the angle of elevation of the chiasm, the width of the chiasm, and the offset between the points of intersection of lines drawn down the centers of the optic nerves and contralateral optic tracts. RESULTS: The mean width of the chiasm was 12.0 ± 1.5 mm (SD), and the mean offset was 4.7 ± 1.4 mm generating a mean offset:width ratio of 0.38 ± 0.09. No chiasm had an offset of zero. The mean incident angle of optic nerves was 56 ± 7°, and for optic tracts, it was 51 ± 7°. CONCLUSIONS: The human optic chiasm is "H" shaped, not "X" shaped. The findings are consistent with nasal fibers decussating an average of 2.4 mm lateral to the midline before travelling in parallel across the midline. This information will inform future models of chiasmal compression.


Assuntos
Imageamento por Ressonância Magnética/métodos , Quiasma Óptico/anatomia & histologia , Adulto , Idoso , Feminino , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Nervo Óptico/anatomia & histologia , Fotomicrografia , Adulto Jovem
9.
J Cutan Pathol ; 48(3): 422-424, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32621564

RESUMO

Pilomatricoma, also known as calcifying epithelioma of Malherbe, is a common benign skin adnexal tumor with differentiation toward hair cortex cells, commonly seen in head and neck region of children. It is usually solitary. Herein, we present a rare case of pilomatricoma with extensive osseous metaplasia as a thigh swelling in an adult female, considered clinically to be a sebaceous cyst. Florid osseous metaplasia is extremely rare in cases of pilomatricoma. To date, fewer than 10 cases of pilomatricoma with extensive osseous metaplasia at different sites have been reported in the English literature.


Assuntos
Doenças do Cabelo/patologia , Metaplasia/patologia , Pilomatrixoma/diagnóstico , Neoplasias Cutâneas/patologia , Adolescente , Adulto , Diferenciação Celular , Criança , Diagnóstico Diferencial , Cisto Epidérmico/diagnóstico , Feminino , Displasia Fibrosa Óssea/complicações , Seguimentos , Cabeça/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Pescoço/patologia , Osteomielite/complicações , Fotomicrografia/métodos , Pilomatrixoma/complicações , Pilomatrixoma/cirurgia , Resultado do Tratamento
10.
J Cutan Pathol ; 48(7): 842-846, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33326119

RESUMO

BACKGROUND: The histopathological diagnosis of MF is challenging, and there is significant overlap with benign inflammatory processes. Clinical features may be relevant in the assessment of skin biopsies. METHODS: We provided photomicrographs to board-certified dermatopathologists and one hematopathologist with and without accompanying clinical photographs and assessed accuracy and confidence in diagnosing MF. RESULTS: We found that access to clinical photographs improved diagnostic accuracy in both MF and non-MF (distractors); the degree of improvement was significantly higher in the non-MF/distractor category. Across all categories, diagnostic confidence level was higher when clinical images were available. CONCLUSION: These findings suggest that clinical images are useful in making an accurate diagnosis of MF, and may be particularly helpful in ruling it out when an inflammatory disorder is clinically suspected.


Assuntos
Inflamação/patologia , Micose Fungoide/diagnóstico , Fotomicrografia/métodos , Neoplasias Cutâneas/patologia , Adulto , Biópsia/métodos , Dermatologistas/psicologia , Diagnóstico Diferencial , Hematologia/estatística & dados numéricos , Humanos , Pessoa de Meia-Idade , Micose Fungoide/patologia , Micose Fungoide/ultraestrutura , Variações Dependentes do Observador , Patologistas/psicologia , Competência Profissional/estatística & dados numéricos , Reprodutibilidade dos Testes , Autoimagem , Pele/patologia
12.
Nat Methods ; 17(11): 1065, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33122858
13.
Ann Diagn Pathol ; 48: 151604, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32877832

RESUMO

The visualization of glycogen deposits in cells and tissues is important for studying glycogen metabolism as well as diagnosis of glycogen storage diseases. Evidence suggests that the demonstration of glycogen can better be enhanced by factors such the choice of fixative and temperature during fixation. Here, we assessed efficacy of neutral buffered formalin (NBF), alcoholic formalin (AF) and paraformaldehyde (PFA) at 4 °C, 37 °C and 40 °C using Periodic Acid Schiff's staining method. Each liver specimen was fixed in NBF and AF while the brain tissues were fixed in NBF, AF and PFA. We found that there was a better PAS staining intensity with the liver tissues fixed in AF compared with NBF. Also, there was no difference in the quality of the staining for tissues fixed in AF at 37 °C, 4 °C and 40 °C, but fixation with NBF at 4 °C gave the best staining quality when compared with 40 °C and 37 °C. Furthermore, hippocampal tissues fixed in AF showed better quality of PAS staining compared with NBF and PFA. A significant increase in staining intensity was observed for PFA when compared with NBF. Superior staining intensity for PAS was observed at 4 °C for hippocampal tissues fixed with NBF, AF and PFA. Taken together our results show that AF at a temperature of 4 °C gave the best result. Hence, glycogen demonstration can better be enhanced by the choice of fixative and temperature during fixation.


Assuntos
Encéfalo/efeitos dos fármacos , Fixadores/farmacologia , Glicogênio/metabolismo , Fígado/efeitos dos fármacos , Animais , Encéfalo/metabolismo , Formaldeído/farmacologia , Doença de Depósito de Glicogênio/diagnóstico , Fígado/metabolismo , Masculino , Reação do Ácido Periódico de Schiff/estatística & dados numéricos , Fotomicrografia/métodos , Polímeros/farmacologia , Ratos , Ratos Wistar , Coloração e Rotulagem/estatística & dados numéricos , Temperatura , Fixação de Tecidos/métodos
14.
Ars pharm ; 61(3): 175-180, jul.-sept. 2020. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-195120

RESUMO

INTRODUCTION: The main aim of present study was to prepare and characterize liposomal formulation of baclofen to improve the effectiveness of the topically applied formulation. METHOD: For the preparation of liposomes, different ratio of lecithin, cholesterol and ethanol were taken but ratio of drug and stearic acid were kept constant and prepared by ethanol injection method. Liposomes were characterized for vesicle size, vesicle shape, entrapment efficiency, in vitro studies, stability studies and in vivo studies. RESULTS: The average particle size of formulated liposome was in the range of 3.98 ± 0.45-4.24 ± 0.65 mim and small unilamellar vesicles with spherical in shape observed. Entrapment efficiency of optimized formulation was 58.67 ± 0.81 %. The maximum % cumulative drug release behaviours were 67.66 ± 5.32 % after 10 h. formulation stored in 4 ± 2 °C temperature shows better stability (64.19 ± 0.26) compared to elevated temperature. Swiss albino mice were used for the in vivo study and exhibit muscle relaxant activity in terms of no. of falls from rota rod apparatus (p value = 0.001). CONCLUSIONS: Baclofen loaded liposomal formulation have shown skeletal muscle relaxant activity in mice suggesting delivery of baclofen from liposomes in the therapeutic range


INTRODUCTION: the use of specific rules to correctly identify ingredients used in cosmetics was essential for their control. This paper analyses the complex process to adapt the INCI terminology between the 1960s and the 1990s. METHOD: analysis of the legislation published in Spain on the control of cosmetic products between the 1940s and the 1990s, focusing on cosmetic's registers and terminologies and nomenclatures used to identify their ingredients. Printed Primary sources, and periodical press have also been consulted. Primary sources have been discussed and contextualized with the help of more recent history of science publications. Results and CONCLUSIONS: The adoption of precise cosmetic nomenclature or terminology was required by health authorities registering these products, as well as for the labelling to inform consumer. The sanitary regulation of cosmetic products was very lax until the development of this industry and its market in Spain in the 1960s. The consolidation of the dermopharmaceutical sector occurred in the 1970s, in part due to the efforts of various pharmaceutical sectors. The gradual introduction of international cosmetic nomenclatures culminated in the 1990s with the official introduction of the INCI terminology in Spain


Assuntos
Baclofeno/química , Lipossomos/química , Lecitinas/química , Composição de Medicamentos , Administração Tópica , Lecitinas/farmacologia , Fármacos Neuromusculares/química , Estabilidade de Medicamentos , Microscopia/métodos , Fotomicrografia
15.
Medicina (Kaunas) ; 56(9)2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32825215

RESUMO

Background and Objectives: There is still considerable controversy regarding the possibility of submitting replanted teeth to orthodontic movement (OM). The purpose of the present study was to evaluate the tissue response after orthodontic movement on replanted teeth. Materials and Methods: Sixty Wistar rats were randomly assigned to four groups (n = 15): G1, replantation without OM after 30 days; G2, replantation with OM after 30 days; G3, replantation without OM after 60 days, and G4, replantation with OM after 60 days. The maxillary left central incisors were extracted and the teeth were stored in milk media. After 30 min, the teeth were replanted and fixed with non-rigid immobilization. All specimens were observed after 30 and 60 days of replantation and then subdivided into two subgroups (with OM or without OM). The animals were euthanized after seven days of the OM started, and the maxillary bone blocks were processed for histological evaluation. Results: The histological results showed periodontal ligament repair in both periods studied without OM; however, ankylosis and root resorption was seen in all orthodontically moved teeth. Conclusions: The orthodontic movement did not favor tissue response in all replanted teeth, regardless of the experimental periods.


Assuntos
Incisivo/patologia , Incisivo/cirurgia , Ligamento Periodontal/patologia , Reabsorção da Raiz/patologia , Técnicas de Movimentação Dentária/efeitos adversos , Reimplante Dentário , Animais , Incisivo/fisiopatologia , Modelos Animais , Fotomicrografia , Complicações Pós-Operatórias/patologia , Distribuição Aleatória , Ratos , Ratos Wistar , Reabsorção da Raiz/etiologia , Avulsão Dentária/cirurgia , Raiz Dentária/patologia
16.
Parasitology ; 147(12): 1375-1380, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32729439

RESUMO

Parasitic castration of bivalves by trematodes is common, and may significantly reduce the reproductive capacity of ecologically important species. Understanding the intensity of infection is desirable, as it can indicate the time that has passed since infection, and influence the host's physiological and reproductive response. In addition, it is useful to know the developmental stage of the trematode, to understand trematode population trends and reproductive success. However, most existing methods (e.g. visually estimating the degree of infection) to assess intensity are approximate only and not reproducible. Here, we present a method to accurately quantify the percentage of bivalve gonad filled with digenean trematode tissue, based on small squashes of gonad tissue rapidly photographed under light microscopy. A maximum of 15 photographs is required to determine the percentage of the whole gonad occupied by trematodes with a minimum of 90% confidence, with smaller mussels requiring fewer. In addition, the stage of trematode infection can be assessed because full sporocysts, spent sporocysts and free cercariae are clearly distinguishable. Although variation exists in the distribution of trematodes in gonad tissue, and thus in the estimate of percentage of the gonad filled with trematodes, this method represents a marked improvement on current coarse assessments of infection which typically focus on binary presence/absence measures. This technique can be used to facilitate a more sophisticated understanding of host-parasite interactions in bivalves, and can inform the conservation and reproductive biology of environmentally crucial species.


Assuntos
Bivalves/parasitologia , Gônadas/parasitologia , Fotomicrografia/métodos , Trematódeos , Animais , Castração , Cercárias/crescimento & desenvolvimento , Cercárias/fisiologia , Estágios do Ciclo de Vida/fisiologia , Trematódeos/crescimento & desenvolvimento , Trematódeos/fisiologia , Infecções por Trematódeos/diagnóstico por imagem , Infecções por Trematódeos/veterinária
17.
Eur. j. anat ; 24(3): 171-191, mayo 2020. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-191467

RESUMO

The kidney plays a vital role in eliminating the toxic substances and drug metabolites from blood. Therefore, it is prone to hazards in case of abuse or overdose of drugs. The aim of the work was to clarify the impact of Moringa oleifera (MO) against the nephrotoxic effect of paracetamol in adult male albino rats. Twenty-eight adult male albino rats were used in this study and divided equally into four groups (G); G1 (control G), G2 (paracetamol-treated animals), G3 (paracetamol- plus high MO-treated G), G4 (high MO-treated rats). Kidney specimens were harvested after anesthetizing the animals, and then processed for histological and DNA comet analysis. Biochemical blood investiga-tions for kidney function were also performed through assessment of serum creatinine (Cr) and blood urea nitrogen (BUN). Administration of para-cetamol resulted in genotoxicity and marked bio-chemical and renal histological changes. It caused a significant increase in Cr and BUN levels and degeneration in both proximal and distal convolut-ed tubules with glomerular changes. Administration of MO with paracetamol led to a noticeable amelioration of previous mentioned changes. Moringa oleifera is suggested to be an effectivenephroprotection against paracetamol-induced damage. Further studies are recommended to con-firm the protective role of MO using large numbers of different animal models before investigating it in humans


No disponible


Assuntos
Animais , Masculino , Ratos , Acetaminofen/efeitos adversos , Intoxicação/veterinária , Rim/anatomia & histologia , Moringa oleifera/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Moringa oleifera/metabolismo , Rim/efeitos dos fármacos , Modelos Animais , Peso Corporal/efeitos dos fármacos , Fotomicrografia
18.
Eur. j. anat ; 24(3): 193-203, mayo 2020. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-191468

RESUMO

The degenerative and inflammatory changes were reported in cardiac tissues of rats exposed to zidovudine (ZDV). This study was designed to ex-amine the histochemical changes in the myocardi-um of adult Wistar rats exposed to ZDV and ad-ministered with methanolic extract of Buchholzia coriacea (MEBC) seed. Forty-eight healthy Wistar rats weighing 150-155 g. were randomly assigned into eight groups of six rats each. Group A served as control and received distilled water; group B received 100 mg/kg of ZDV; group C received 600 mg/kg of MEBC; group D received 100 mg/kg of vitamin C; group E received 100 mg/kg of vitamin C and ZDV; group F received 150 mg/kg of MEBC and 100 mg/kg of ZDV; group G received 300 mg/kg of MEBC and 100 mg/kg of ZDV, and group H received 600 mg/kg of MEBC and 100 mg/kg of ZDV. Treatment lasted for a period of 56 days. Blood was collected separately into clean capped plain tubes for biochemical parameters. Heartswere excised, fixed in 10% formal saline and pro-cessed for histology. ZDV induced a significant increase in the serum concentration of Nitric Oxide (NO) and Cardiac Troponin I (cTnI) in the ZDV-alone group when compared to control (p < 0.05). Also, there was reduction in activity of the Glutathi-one reductase (GR) enzyme in the ZDV-alone group relative to control (P = 0.0006, F = 7.0). Distor-tion of the cross banding pattern of cardiac muscle fibres in ZDV-alone group was manifested. These effects were reversed by administration of MEBC compared to vitamin C group


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Assuntos
Animais , Ratos , Ventrículos do Coração/anatomia & histologia , Metanol/administração & dosagem , Zidovudina/efeitos adversos , Cardiotoxicidade/tratamento farmacológico , Cardiotoxicidade/veterinária , Coração/anatomia & histologia , Capparaceae , Extratos Vegetais/administração & dosagem , Ventrículos do Coração/efeitos dos fármacos , Zidovudina/administração & dosagem , Ratos Wistar/anatomia & histologia , Coração/efeitos dos fármacos , Técnicas Histológicas , Fotomicrografia , Antioxidantes/administração & dosagem , Sementes
19.
Acta toxicol. argent ; 28(1): 19-22, Apr. 2020. graf
Artigo em Espanhol | LILACS | ID: biblio-1248665

RESUMO

Resumen La micrografía analítica es una herramienta útil para la identificación de restos vegetales en muestras de material trozado o molido que no podrían ser identificadas de forma morfológica. El objetivo de este estudio es conocer los caracteres mi- crográficos de las semillas de Cebil (Anadenanthera colubrina var. cebil (Griseb.) Altschul, Leguminosae) y Chamico (Datura ferox L., Solanaceae), con el fin de proporcionar parámetros empleables para su identificación en un contexto forense y toxicológico. Los caracteres micrográficos relacionados con el tegumento exterior y las esclereidas fueron los más indicados para diferenciar entre ambas especies.


Abstract Analytical micrography is a useful tool for the identification of plant parts that can't be identified for its morphological characters. The aim of this work is to obtain micrographic characters of Cebil (Anadenanthera colubrina var. cebil (Griseb.) Altschul, Leguminosae) and Chamico (Datura ferox L., Solanaceae) seeds for its identification in a toxicological and forensic context. The most suitable micrographic features were the ones related with exterior testa and the stone cells.


Assuntos
Sementes/citologia , Solanaceae/citologia , Alucinógenos , Fabaceae/citologia , Argentina , Intoxicação por Plantas/diagnóstico , Fotomicrografia/métodos
20.
Neurocirugía (Soc. Luso-Esp. Neurocir.) ; 31(2): 76-86, mar.-abr. 2020. ilus, grab, tab
Artigo em Inglês | IBECS | ID: ibc-190375

RESUMO

INTRODUCTION AND OBJECTIVES: Angiolipomas of the spinal canal are a rare condition of unknown origin. They are considered histologically benign; however, some have the potential to infiltrate adjacent structures. The aim of this systematic review was to suggest a potential mechanism for the pathogenesis of spinal angiolipomas, along with a useful approach for their preoperative management. MATERIALS AND METHODS: A literature review of cases of spinal angiolipoma was performed. In addition, two of the cases encountered in our practice are presented. The first case refers to a 35-year-old male patient with a history of spinal fusion because of a T9 fracture, while the second concerns a 46-year-old male patient with an epidural mass extending outside the spinal canal, who underwent fine needle biopsy and embolisation of its feeding vessel. RESULTS: From the review of the literature performed, we were unable to identify any correlation between the infiltrative potential and the patients' demographic and tumour characteristics. CONCLUSIONS: Angiolipomas are considered to be sporadic, yet theories concerning their pathogenesis include reaction to harmful stimuli and congenital malformation of the adipose tissue. Fine needle biopsy may be mistakenly considered non-diagnostic, due to the presence of well-differentiated adipocytes


INTRODUCCIÓN Y OBJETIVOS: Los angiolipomas del canal vertebral son una enfermedad rara de origen desconocido. Se consideran histológicamente benignos, aunque en algunos casos existe la posibilidad de que se infiltren en estructuras adyacentes. El objetivo de esta revisión sistemática es sugerir un posible mecanismo para la patogenia de los angiolipomas espinales, junto con un enfoque útil para su tratamiento preoperatorio. Materiales y métodos: Se realizó una búsqueda bibliográfica de los casos de angiolipomas espinales. Además, se presentan 2 de los casos encontrados en nuestra práctica clínica. El primer caso corresponde a un paciente varón de 35 años con antecedentes de artrodesis vertebral debido a una fractura en T9, mientras que el segundo corresponde a un paciente varón de 46 años con una masa epidural que se extendía fuera del canal vertebral, al que se realizó una biopsia con aguja fina y una embolización del vaso nutricio. RESULTADOS: A partir de la revisión bibliográfica realizada, no pudimos identificar ninguna correlación entre el potencial de infiltración, los datos demográficos y las características de los tumores de los pacientes. CONCLUSIONES: Los angiolipomas se consideran esporádicos, existiendo, no obstante, teorías referentes a su patogenia que incluyen la reacción a estímulos nocivos y la malformación congénita del tejido adiposo. La biopsia con aguja fina puede considerarse erróneamente como no diagnóstica, debido a la presencia de adipocitos bien diferenciados


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Angiolipoma/cirurgia , Medula Espinal/cirurgia , Neoplasias da Medula Espinal/cirurgia , Angiolipoma/diagnóstico por imagem , Biópsia , Embolização Terapêutica , Tomografia Computadorizada por Raios X , Espectroscopia de Ressonância Magnética , Meios de Contraste , Fotomicrografia , Medula Espinal/patologia , Diagnóstico Diferencial , Neoplasias da Medula Espinal/diagnóstico por imagem
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