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1.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 39(2): 398-404, 2022 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-35523562

RESUMO

This study aims to explore the potential of polyaspartic acid grafted dopamine copolymer (PAsp- g-DA) chelated Fe 3+ for magnetic resonance imaging (MRI) visual photothermal therapy. Polyaspartic acid grafted copolymer of covalently grafted dopamine and polyethylene glycol (PAsp- g-DA/PEG) was obtained by the ammonolysis reaction of poly succinimide (PSI), and then chelated with Fe 3+ in aqueous solution. The relaxivity in vitro, magnetic resonance imaging enhancement in vivo and photothermal conversion effect at 808 nm were investigated. The results showed that polymeric iron coordination had good near-infrared absorption and photothermal conversion properties, good magnetic resonance enhancement effect, and good longitudinal relaxation efficiency under different magnetic field intensities. In summary, this study provides a new magnetic resonance visual photothermal therapeutic agent and a new research idea for the research in related fields.


Assuntos
Nanopartículas , Polímeros , Dopamina , Imageamento por Ressonância Magnética/métodos , Peptídeos , Fototerapia , Terapia Fototérmica
2.
ACS Biomater Sci Eng ; 8(5): 1892-1906, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35404565

RESUMO

Organic near-infrared fluorescent dye mediated photothermal therapy (PTT) and photodynamic therapy (PDT) suffer from heat shock response, since, heat shock proteins (HSPs) are overexpressed and can repair the proteins damaged by PTT and PDT. Starvation therapy by glucose oxide (GOx) can inhibit the heat shock response by limiting the energy supply. However, the delivery of sufficient and active GOx remains a challenge. To solve this problem, we utilize liposomes as drug carriers and prepare GOx loaded liposome (GOx@Lipo) with a high drug loading content (12.0%) and high enzymatic activity. The successful delivery of GOx shows excellent inhibition of HSPs and enhances PTT and PDT. Additionally, we apply the same liposome formulation to load near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbo cyanine iodide (DiR) and prepare DiR contained liposomes (DiR@Lipo) for PTT and PDT. The liposomal formulation substantially enhances the PTT and PDT properties of DiR as well as the cellular uptake and tumor accumulation. Finally, the combination therapy shows excellent tumor inhibition on 4T1 tumor-bearing mice. Interestingly, we also find that the starvation therapy can efficiently inhibit tumor metastasis, which is probably due to the immunogenic effect. Our work presents a biocompatible and effective carrier for the combination of starvation therapy and phototherapy, emphasizing the importance of auxiliary starvation therapy against tumor metastasis and offering important guidance for clinical PTT and PDT.


Assuntos
Neoplasias , Fotoquimioterapia , Animais , Glucose Oxidase/uso terapêutico , Lipossomos/uso terapêutico , Camundongos , Neoplasias/tratamento farmacológico , Terapia Fototérmica
3.
Inorg Chem ; 61(18): 6852-6860, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35477242

RESUMO

Combined photothermal/photodynamic therapy is a promising strategy to achieve an enhanced anticancer effect. However, hypoxia is one of the representative characteristics of the microenvironment of solid tumors, which not only attenuates the therapeutic effects but also promotes tumor invasion and metastasis. Herein, a PtBi-ß-CD-Ce6 nanoplatform for the generation of sustained O2 was constructed for more effective tumor therapy. In detail, the catalase (CAT)-like nanozyme, PtBi, which could decompose H2O2 to produce O2, was modified with ß-cyclodextrin (ß-CD). O2 would be converted into 1O2 by PtBi-ß-CD-Ce6 for enhanced photodynamic therapy (PDT) under 650 nm laser irradiation. In addition, by reason of excellent absorption in the near-infrared-II (NIR-II) region, PtBi-ß-CD-Ce6 was used for photoacoustic imaging (PA) and photothermal imaging (PT)-guided photothermal therapy (PTT) in the NIR-II biowindow. Furthermore, PtBi-ß-CD-Ce6 could be elected to serve as a contrast agent for X-ray computed tomography (CT) imaging due to the apparent X-ray attenuation capability of the Pt and Bi elements themselves. Therefore, by integrating the advantages of overcoming the hypoxia function and photothermal effect into a single nanoplatform, PtBi-ß-CD-Ce6 showed an immense possibility in multimodal imaging-guided combined PDT/PTT.


Assuntos
Nanopartículas , Neoplasias , Fotoquimioterapia , beta-Ciclodextrinas , Linhagem Celular Tumoral , Humanos , Peróxido de Hidrogênio , Hipóxia/tratamento farmacológico , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Terapia Fototérmica , Microambiente Tumoral , beta-Ciclodextrinas/farmacologia , beta-Ciclodextrinas/uso terapêutico
4.
Mol Pharm ; 19(5): 1449-1457, 2022 05 02.
Artigo em Inglês | MEDLINE | ID: mdl-35388697

RESUMO

Cancer is one of the main diseases threatening human health. Immunotherapy, in which cancer is treated by activating immune cells and inducing the body's immune response, has rapidly developed. Photothermal therapy (PTT), a new treatment method that ablates tumors by light irradiation, has attracted great attention for its good therapeutic effect and low toxic side effects. In the present study, we combined photothermal and immunotherapy to design a novel nanoparticle delivery system by loading indoleamine 2,3-dioxygenase (IDO) inhibitors and toll-like receptor (TLR) agonists into polydopamine (PDA) nanoparticles coated with polyethylene imine (PEI). This delivery system has the advantages of high homogeneity, good stability, excellent biocompatibility, and low toxicity. In vitro antitumor studies showed that the system effectively inhibited the proliferation of mouse breast carcinoma cells and induced cell apoptosis. From the in vivo studies, we found that the system inhibited the growth of mouse breast carcinoma, facilitated the maturation of antigen-presenting cells, promoted T lymphocyte differentiation, and induced the body's immune response. The present study developed a dual functional drug delivery system combining photothermal therapy and immunotherapy to efficiently improve antitumor therapy with potential clinical application.


Assuntos
Neoplasias da Mama , Nanopartículas , Adjuvantes Imunológicos , Animais , Neoplasias da Mama/terapia , Linhagem Celular Tumoral , Sistemas de Liberação de Medicamentos , Feminino , Humanos , Imunoterapia , Camundongos , Fototerapia/métodos , Terapia Fototérmica
5.
Anal Chem ; 94(17): 6463-6472, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35435669

RESUMO

Raman thermometry based on surface-enhanced Raman scattering has been developed using nanopipettes in cancer cell photothermal therapy (PTT). Gold nanorods (AuNRs) are robustly epoxied on glass pipettes with a high surface coverage of ∼95% and less than 10 nm-wide nanogaps for intracellular thermometry and photothermal cancer therapy. The temperature changes could be estimated from the N≡C band shifts of 4-fluorophenyl isocyanide (FPNC)-adsorbed AuNRs on the Raman thermometry nanopipette (RTN) surfaces. An intracellular temperature change of ∼2.7 °C produced by altering the [Ca2+] in A431 cells was detected using the RTN in vitro, as checked from fura-2 acetoxymethyl ester (fura-2 AM) fluorescence images. For in vivo experiments, local temperature rises of ∼19.2 °C were observed in the mouse skin, whereas infrared camera images could not tract due to spatial resolution. In addition, a tumor growth suppression was observed in the PTT processes after an administration of the three AuNR-coated nanopipettes combined with a 671 nm laser irradiation for 5 min in 30 days. These results demonstrate not only the localized temperature sensing ability of FPNC-tagged AuNR nanopipettes in cell biology but also anti-cancer effects in photothermal cancer therapy.


Assuntos
Nanotubos , Neoplasias , Termometria , Animais , Linhagem Celular Tumoral , Fura-2 , Ouro , Camundongos , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Neoplasias/terapia , Terapia Fototérmica
6.
Dalton Trans ; 51(17): 6846-6854, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35438705

RESUMO

Ruthenium polypyridyl complexes have been widely used as bioprobes and photosensitizers. However, several disadvantages including slow cellular uptake, nonspecific binding with biomolecules and toxicity limit their applications. In this study, a nanocarrier of human serum albumin coated gold nanorods was developed to deliver a ruthenium photosensitizer for PDT/PTT combination therapy. The HSA coating endowed the nanodrug with high biocompatibility and stability under physiological conditions. Ru-GNR-HSANPs generate 1O2 and hydroxyl radicals to kill cancer cells under blue light irradiation, and exhibit excellent photothermal anticancer effects under 808 nm light irradiation. Significant synergistic anticancer effects were achieved by combined PDT/PTT therapy. Importantly, Ru-GNR-HSANPs can have the synergistic PDT/PTT functions with no need of drug release from the carrier.


Assuntos
Nanotubos , Fotoquimioterapia , Rutênio , Ouro/química , Ouro/farmacologia , Humanos , Nanotubos/química , Fármacos Fotossensibilizantes/química , Terapia Fototérmica , Rutênio/química , Rutênio/farmacologia
7.
Anal Chem ; 94(17): 6599-6606, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35445600

RESUMO

Developing an intelligent theranostic nanoplatform with satisfied diagnostic accuracy and therapeutic efficiency holds great promise for personalized nanomedicine. Herein, we constructed a smart nanodevice for the accurate diagnosis of endogenous cancer microRNA (miRNA) biomarkers and efficient photothermal therapy (PTT). The nanodevice was composed of polydopamine (PDA)-functionalized CuS nanosheets (CuS@PDA NSs) and three elaborate DNA hairpin probes (TDHPs). The CuS@PDA NSs acted as efficient delivery vehicles and photothermal agents. They provided a large surface area available for an efficient and facile loading of TDHPs and a high-fluorescence (FL) quenching performance to achieve an ultralow background signal. The intracellular miRNA triggered TDHPs to assemble into three-arm branched junction structures for a strong fluorescence recovery as output signals to discriminate cancer cells from normal cells with an excellent sensitivity. The CuS@PAD NSs showed a good photothermal conversion efficiency in the near-infrared II (NIR II) region to mediate a good photothermal performance to kill cancer cells. A remarkable antitumor therapeutic effect was achieved in vivo. This work integrated highly sensitive detection to endogenous cancer biomarkers and valid therapeutic potency to tumor-bearing mice, indicating its promising biomedical applications.


Assuntos
MicroRNAs , Nanopartículas , Neoplasias , Animais , Sondas de DNA , Camundongos , MicroRNAs/genética , Nanopartículas/química , Neoplasias/tratamento farmacológico , Neoplasias/terapia , Fototerapia , Terapia Fototérmica
8.
Drug Deliv ; 29(1): 1312-1325, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35475384

RESUMO

Photothermal therapy (PTT) has become one of the most promising therapies in cancer treatment as its noninvasiveness, high selectivity, and favorable compliance in clinic. However, tumor thermotolerance and distal metastasis reduce its efficacy, becoming the bottleneck of applying PTT in clinic. In this study, a chidamide-loaded magnetic polypyrrole nanocomposite (CMPP) has been fabricated as a visualized cancer photothermal agent (PTA) to counter tumor thermotolerance and metastasis. The efficacy of CMPP was characterized by in vitro and in vivo assays. As a result, this kind of magnetic polypyrrole nanocomposites were black spherical nanoparticles, possessing a favorable photothermal effect and the suitable particle size of 176.97 ± 1.45 nm with a chidamide loading rate of 12.92 ± 0.45%. Besides, comparing with PTT, CMPP exhibited significantly higher cytotoxicity and cellular apoptosis rate in two tumor cell lines (B16-F10 and HepG2). In vivo study, the mice showed obvious near-infrared (NIR) and magnetic resonance imaging (MRI) dual-modal imaging at tumor sites and sentinel lymph nodes (SLNs); on the other hand, magnetic targeting guided CMPP achieved a cure level on melanoma-bearing mice through preventing metastasis and thermotolerance. Overall, with high loading efficiency of chidamide and strong magnetic targeting to tumor sites and SLNs, CMPP could significantly raise efficiency of PTT by targeting tumor thermotolerance and metastasis, and this strategy may be exploited therapeutically to upgrade PTT with MPP as one of appropriate carriers for histone deacetylase inhibitors (HDACis).


Assuntos
Neoplasias , Termotolerância , Aminopiridinas , Animais , Benzamidas , Imageamento por Ressonância Magnética/métodos , Camundongos , Neoplasias/tratamento farmacológico , Fototerapia , Terapia Fototérmica , Polímeros/química , Pirróis
9.
Front Endocrinol (Lausanne) ; 13: 872411, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35464050

RESUMO

Since 2019, coronavirus disease 2019 (COVID-19) has swept the world and become a new virus threatening the health of all mankind. The survey found that prostate cancer accounts for one in three male cancer patients infected with COVID-19. This undoubtedly makes prostate cancer patients face a more difficult situation. Prostate cancer is the second most harmful malignant tumor in men because of its insidious onset, easy metastasis, and easy development into castration-resistant prostate cancer even after treatment. Due to its high immunogenicity and a small number of specific infiltrating T cells with tumor-associated antigens in the tissue, it is difficult to obtain a good therapeutic effect with immune checkpoint blocking therapy alone. Therefore, in the current study, we developed a platform carrying Doxorubicin (DOX)-loaded black phosphate nanometer combined with photothermal therapy (PTT) and found this drug combination stimulated the immungentic cell death (ICD) process in PC-3 cells and DC maturation. More importantly, zinc ions have a good immunomodulatory function against infectious diseases, and can improve the killing ability of the nanosystem against prostate cancer cells. The introduction of Aptamer (Apt) enhances the targeting of the entire nanomedicine. We hope that this excellent combination will lead to effective treatment strategies for prostate cancer patients infected with COVID-19.


Assuntos
COVID-19 , Neoplasias da Próstata , COVID-19/terapia , Humanos , Masculino , Fósforo , Terapia Fototérmica , Neoplasias da Próstata/complicações , Neoplasias da Próstata/terapia , Zinco
10.
ACS Appl Mater Interfaces ; 14(17): 19192-19203, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35438482

RESUMO

Photoacoustic imaging (PAI) guided photothermal therapy (PTT) can realize real-time diagnosis and in situ treatment of cancer at the same time. Absorption in the near-infrared (NIR) region with large molar extinction coefficient (ε) and high value of photothermal conversion efficiency (PCE) are key prerequisites for photothermal agents (PTAs) to realize dual PAI and PTT treatments. Squaraines have stable quinoid structures with strong planarity and rigidity, in favor of the NIR absorption and high ε values. On the other hand, azulene derivatives mostly have very faint fluorescence emission, which is beneficial for photothermal transformation. Herein, two azulene-containing squaraines Az-SQ-1 and Az-SQ-2 are synthesized as high-performance PTAs. In comparison with Az-SQ-1, Az-SQ-2 possesses larger εmax of 3 × 105 M-1 cm-1 at 780 nm in organic solution and higher PCE of 53.2% in the form of nanoparticles under 808 nm laser irradiation. Accordingly, Az-SQ-2 NPs present stronger photoacoustic signals (about 15.1-times the background signal) and more efficient suppression of tumor growth. Our research indicates that the introduction of azulene unit to traditional NIR dyes is a simple but effective approach to obtain outstanding PTAs in the aspect of phototheranostics.


Assuntos
Nanopartículas , Neoplasias , Técnicas Fotoacústicas , Azulenos/farmacologia , Ciclobutanos , Humanos , Nanopartículas/química , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fenóis , Técnicas Fotoacústicas/métodos , Fototerapia/métodos , Terapia Fototérmica , Nanomedicina Teranóstica/métodos
11.
ACS Appl Mater Interfaces ; 14(17): 19081-19090, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35442630

RESUMO

Single-atom nanozyme (SAzyme) systems have shown great potential in tumor therapy. A multifunctional SAzyme not only possesses high catalytic activity but also can be used as photothermal agents in photothermal therapy (PTT). Furthermore, it is also imperative to overcome tumor thermal resistance in SAzyme-based PTT so that PTT under a mild temperature is achievable. Herein, a novel platelet membrane (PM)-coated mesoporous Fe single-atom nanozyme (Fe-SAzyme) was formulated to solve these issues. The PM-coated mesoporous Fe-SAzyme (PMS) showed a satisfactory NIR-II photothermal performance, high peroxidase (POD) activity, and good tumor-targeting ability. In addition, PMS may be used as a carrier for protein drugs owing to its inner mesoporous structure. In vitro experiments showed that PMS could inhibit the expression of heat shock protein (HSP) by damaging the mitochondria, thereby finally improving the effect of mild-temperature PTT. Moreover, in vivo results showed that PMS could efficiently accumulate in tumor sites and suppress tumor growth with minimal toxicity in major organs. To the best of our knowledge, this study is the first report of a biomimetic mesoporous Fe-SAzyme used to achieve mitochondrial damage-mediated mild-temperature PTT. The study provides new promising ideas for designing other SAzyme systems for cancer treatment.


Assuntos
Nanopartículas , Neoplasias , Catálise , Linhagem Celular Tumoral , Humanos , Neoplasias/tratamento farmacológico , Peroxidase , Fototerapia , Terapia Fototérmica , Temperatura
12.
Carbohydr Polym ; 288: 119382, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35450644

RESUMO

Numerous protein-based multifunctional nanomaterials have been studied for biomedical applications. However, the inherent immunogenicity and activation of the complement system of protein-based nanomaterials may cause immunological responses, further leading to hypersensitivity and/or allergic reactions in patients. Herein, carboxymethyl chitosan (CMC) and KMnO4 are used to develop polysaccharide-based MnO2 (CM) nanoparticles as facile, effective and low-immunogenicity nanomaterials for magnetic resonance imaging (MRI)-guided photothermal therapy (PTT). As a proof of concept, indocyanine green (ICG) is loaded in the CM nanoplatform to form a CMC-MnO2-ICG compound (CMI) for MRI-guided photothermal therapy. In vitro studies show that the proposed CMC-based nanomaterials exhibit excellent hemocompatibility and no significant complement activation. The in vivo biosafety experiments also show that the CMC-based nanomaterials have low immunogenicity and will not cause tissue damage. Moreover, the prepared CMI nanoparticles (NPs) possess outstanding photothermal properties and high longitudinal relaxivity (8.47 mM-1 s-1), which achieve successful MRI-guided PTT in tumor-bearing mice. This work draws increasing attention to the hemocompatibility and immunogenicity of high-performance nanoprobes for biomedical applications.


Assuntos
Quitosana , Nanopartículas , Nanoestruturas , Animais , Humanos , Verde de Indocianina , Imageamento por Ressonância Magnética , Compostos de Manganês , Camundongos , Nanoestruturas/uso terapêutico , Óxidos , Terapia Fototérmica
13.
J Colloid Interface Sci ; 619: 348-358, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35398765

RESUMO

Due to development of surgical techniques and intraocular lens (IOL) implants, vision can often be restored in cataracts patients. However, posterior capsular opacification (PCO) has become the most common and challenging complication in cataracts surgery. While various approaches such as surface modification and drug prophylaxis have been investigated to prevent PCO development, there is no standard treatment that is sufficiently safe and effective to meet clinical demands. Near-infrared (NIR) light-triggered photothermal therapy is an attractive noninvasive treatment for PCO prophylaxis. We fabricated a new type of IOL with excellent biocompatibility, stability, and photothermal conversion property. Polyethyleneimine (PEI) and graphene oxide (GO) were layer-by-layer assembled on model polymethylmethacrylate and IOL substrates, and the thickness, surface roughness, and wettability of the substrates with different numbers of bilayers were evaluated. After the reduction of GO to reduced GO (rGO), a rGO/PEI multilayer thin film with good stability and photothermal conversion capability was obtained. The rGO/PEI multilayer coating was able to induce apoptosis in lens epithelium cells under 808-nm NIR laser irradiation in vitro. Finally, rGO@IOL was implanted into rabbit eyes, and the biocompatibility and ability to prevent PCO were evaluated for 5 weeks. The rGO@IOL implant exhibited excellent PCO prevention ability with the assistance of NIR irradiation and did not induce obvious pathological effects in surrounding healthy tissues. The rGO@IOL implant with good biocompatibility, good physicochemical stability, and excellent photothermal conversion property shows promise for clinical application in PCO prophylaxis.


Assuntos
Opacificação da Cápsula , Lentes Intraoculares , Animais , Opacificação da Cápsula/etiologia , Opacificação da Cápsula/prevenção & controle , Células Epiteliais , Grafite , Humanos , Lentes Intraoculares/efeitos adversos , Terapia Fototérmica , Coelhos
14.
Drug Deliv ; 29(1): 1201-1211, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35403518

RESUMO

Gastric cancer (GC) is a serious disease with high morbidity and mortality rates worldwide. Chemotherapy plays a key role in GC treatment, while inevitable drug resistance and systematic side effects hinder its clinical application. Fenton chemistry-based chemodynamic therapy (CDT) has been used as a strategy for cancer ferroptosis, and the CDT efficiency could be enhanced by photothermal therapy (PTT). With the trend of treatment and diagnosis integration, the combination of magnetic resonance imaging (MRI) and CDT/PTT exhibits enormous progress. Herein, we constructed a platform based on PEGylated manganese-containing polydopamine (PDA) nanoparticles, named as PEG-PDA@Mn (PP@Mn) NPs. The PP@Mn NPs were stable and globular. Furthermore, they demonstrated near-infrared (NIR)-triggered PTT and Fenton-like reaction-based CDT effects and T1-weighted MRI capabilities. According to in vitro studies, the PP@Mn NPs trigger ferroptosis in cancer cells by producing abundant reactive oxygen species (ROS) via a Fenton-like reaction combined with PTT. Furthermore, in vivo studies showed that, under MRI guidance, the PP@Mn NPs combined with the PTT at the tumor region, have CDT anti-tumor effect. In conclusion, the PP@Mn NPs could provide an effective strategy for CDT/PTT synergistic ferroptosis therapy for GC.


Assuntos
Ferroptose , Nanopartículas , Neoplasias , Neoplasias Gástricas , Linhagem Celular Tumoral , Humanos , Indóis , Imageamento por Ressonância Magnética , Manganês , Neoplasias/tratamento farmacológico , Terapia Fototérmica , Polímeros , Neoplasias Gástricas/diagnóstico por imagem , Neoplasias Gástricas/tratamento farmacológico , Nanomedicina Teranóstica/métodos
15.
Biomater Sci ; 10(9): 2370-2383, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35383799

RESUMO

A combination of photothermal therapy (PTT) and chemotherapy is an emerging therapeutic strategy with promising clinical prospects in cancer treatment. Despite the huge progress achieved in the past years, a number of obstacles still hamper the therapeutic efficacy of this synergistic modality such as uneven heat distribution, lack of targetability of anti-cancer agents and dosage-related side effects. Thus, developing a nanoplatform for targeted drug delivery against cancer is of great necessity. Herein, a lipid-polymer hybrid nanosystem (LP/ID) based on polyethyleneimine (PEI)-lecithin-polyethylene glycol (PEG) was fabricated to co-load indocyanine green (ICG) and dichloroacetate (DCA) for combined photothermal/chemotherapy. DCA and ICG were linked to the PEI backbone to form a dense hydrophobic core through amide bonds and electrostatic interactions, which increased the payload of DCA and ICG as well as achieved enzyme-responsive drug release because of the overexpressed amidase in tumor cells. Lecithin and DSPE-PEG2000 self-assembled around the hydrophobic complexes to obtain prolonged blood circulation and attenuated systemic toxicity of the hybrid nanosystem. The prepared LP/ID exhibited favourable stability in a physiological environment, good tumor imaging properties, and satisfactory photothermal/chemotherapeutic performance. Moreover, LP/ID could also enhance the cellular uptake and tumor retention capacity in comparison with free drug administration. Notably, by co-loading two therapeutic agents with different anti-cancer mechanisms, an obvious inhibitory effect on tumor growth was observed with negligible damage to normal tissues and organs because of the synergistic photothermal/chemotherapy effect, indicating the great potential of LP/ID as a robust nanoplatform for cancer treatment.


Assuntos
Hipertermia Induzida , Nanopartículas , Neoplasias , Linhagem Celular Tumoral , Doxorrubicina/química , Hipertermia Induzida/métodos , Verde de Indocianina/química , Lecitinas , Nanopartículas/química , Neoplasias/tratamento farmacológico , Fototerapia/métodos , Terapia Fototérmica , Polietilenoimina , Polímeros
16.
Biomater Sci ; 10(7): 1831-1843, 2022 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-35253030

RESUMO

Chemotherapy is a conventional treatment method for metastatic bone cancer, but it has limitations, such as lower drug-targeting of bone tissues and serious side effects. Bone metastasis almost always occurs in advanced cancer, and most patients in this period have strong drug resistance, which further worsens the curative effect. To address the above-mentioned difficulties, a drug delivery platform is proposed in this paper that accomplishes the bone-targeting of drugs to efficiently inhibit tumors. First, the anti-cancer drugs 5-fluorouracil (5-Fu) and indocyanine green (ICG) were loaded into a zeolitic imidazolate framework (ZIF-90) to form 5-Fu/ICG@ZIF-90. Polyethylene glycol with zoledronic acid (ZOL) was encapsulated using 5-Fu/ICG@ZIF-90 to synthesize 5-Fu/ICG@ZIF-90-PEG-ZOL nanoparticles, which showed dimensional stability, good thermal stability, and bone-targeting ability. Second, the in vitro anti-cancer activity of the designed platform was investigated using cytotoxicity, apoptosis, live-dead staining, cell cycle, and cell ultrathin section analysis. The results indicated that the nanoparticles inhibited MCF-7 cell activity when chemotherapy was combined with PTT. Finally, H&E staining and TUNEL detection were performed in mouse organs and tumors. The nanoparticles combined with photothermal therapy (PTT) and triggered by near-infrared irradiation induce apoptosis of tumor cells in vivo, displaying a better efficacy of combined chemotherapy and photothermal therapy. Experiments conducted on the 5-Fu/ICG@ZIF-90-PEG-ZOL nanoparticles demonstrated their promising performance for cancer bone metastasis inhibition.


Assuntos
Neoplasias Ósseas , Estruturas Metalorgânicas , Nanopartículas , Animais , Neoplasias Ósseas/tratamento farmacológico , Osso e Ossos , Linhagem Celular Tumoral , Humanos , Camundongos , Terapia Fototérmica , Ácido Zoledrônico/farmacologia
17.
J Nanobiotechnology ; 20(1): 121, 2022 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-35264199

RESUMO

Optically active nanostructures consisting of organic compounds and metallic support have shown great promise in phototherapy due to their increased light absorption capacity and high energy conversion. Herein, we conjugated chlorophyll (Chl) to vanadium carbide (V2C) nanosheets for combined photodynamic/photothermal therapy (PDT/PTT), which reserves the advantages of each modality while minimizing the side effects to achieve an improved therapeutic effect. In this system, the Chl from Leptolyngbya JSC-1 extracts acted as an efficient light-harvest antenna in a wide NIR range and photosensitizers (PSs) for oxygen self-generation hypoxia-relief PDT. The available large surface of two-dimensional (2D) V2C showed high Chl loading efficiency, and the interaction between organic Chl and metallic V2C led to energy conversion efficiency high to 78%. Thus, the Chl/ V2C nanostructure showed advanced performance in vitro cell line killing and completely ablated tumors in vivo with 100% survival rate under a single NIR irradiation. Our results suggest that the artificial optical Chl/V2C nanostructure will benefit photocatalytic tumor eradication clinic application.


Assuntos
Nanoestruturas , Neoplasias , Fotoquimioterapia , Linhagem Celular Tumoral , Clorofila/farmacologia , Humanos , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Fototerapia , Terapia Fototérmica , Vanádio/química , Vanádio/uso terapêutico
18.
Small ; 18(15): e2108055, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35253981

RESUMO

Radical-containing frameworks (RCFs) have emerged as promising functional materials in various fields due to the combination of the highly ordered frame structure and the fascinating property of organic radicals. Here, the first example of radical-containing supramolecular organic frameworks (SOFs) fabricated by the chaotropic effect between closo-dodecaborate cluster (B12 H12 2- ) and 2,4,6-tri(4-pyridyl)-1,3,5-triazine (TPT3+ ) is presented. The SOFs can be easily synthesized by stirring the B12 H12 2- and the TPT3+ in aqueous solution through self-assembly. Upon 435 nm light irradiation, the SOFs exhibits photochromic behavior from slight yellow (SOF-1) to dark purple (SOF-2). Electron paramagnetic resonance spectroscopy also reveals that stable radicals are generated in situ after light irradiation. Powder X-ray diffraction demonstrates the SOFs maintain their structural stabilities upon light irradiation. More interestingly, the radical-containing SOFs exhibit efficient photothermal effect under 660 nm light irradiation, which can be applied as photothermal agent for antibacterial application both in vitro and in vivo. This work highlights the construction of RCFs through supramolecular self-assembly, which may arouse applications in energy, catalysis, photoluminescence, and biomedical fields.


Assuntos
Terapia Fototérmica , Catálise
19.
Langmuir ; 38(12): 3755-3764, 2022 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-35291761

RESUMO

Isotropic gold nanoparticles (AuNPs) can generate a plasma-plasma interaction when aggregating and can also produce ideal photothermal effects. Some studies have designed ATP-responsive nanodrug delivery systems by taking advantage of the differences between internal and external ATP in tumor cells, but few studies have focused on the photothermal effects of ATP-induced AuNP aggregation in tumors. Here, a triple-helix probe (THP) molecular switch and MUC1 aptamer-functionalized AuNPs were constructed for fluorescence imaging analysis and photothermal therapy (PTT). The MUC1 aptamer guides THP-AuNP targeting in tumor cells, followed by the high concentration of ATP inducing structural changes in triple-helix probes and causing the intracellular aggregation of AuNPs, which cannot escape from the tumor site, enabling tumor imaging while performing PTT. Therefore, the designed THP-AuNPs have promising applications in fluorescence imaging and PTT.


Assuntos
Nanopartículas Metálicas , Neoplasias , Trifosfato de Adenosina , Ouro/química , Humanos , Nanopartículas Metálicas/química , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Imagem Óptica , Terapia Fototérmica
20.
J Mater Chem B ; 10(15): 2828-2843, 2022 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-35316319

RESUMO

Multi-drug resistance (MDR) is a complicated cellular defense mechanism for tumor cells to resist chemotherapy drugs, which is also the main cause of chemotherapy failure. In this study, a local injectable hydrogel delivery system was used to construct an on-demand sustained-release platform with the advantages of chemotherapy, photothermal therapy (PTT), and magnetic resonance imaging (MRI). It could achieve synergistic chemo-photothermal therapy and real-time evaluation of the therapeutic effects (via MRI) for MDR hepatocellular carcinoma (HCC). Furthermore, after a single administration, the prepared hydrogel with a theranostic nanoprobe could release the therapeutic agents on demand for up to 14 d. Firstly, doxorubicin (DOX) and gold-manganese oxide (Au-MnO) nanoparticles (NPs) were incorporated into liposome-based self-assembled micelles, then loaded into the thermosensitive hydrogel (F127) to form DOX@Au-MnO-L NPs/F127 hydrogel (DAML/H). The prepared NP complex showed a spherical morphology with a narrow size distribution. The prepared hydrogel drug delivery system had injectable properties and stable photothermal conversion. Both the DOX@Au-MnO-L NPs and DAML/H showed controlled drug release under near infrared (NIR) laser irradiation. The in vitro MRI studies indicated that the prepared DAML/H had a high relaxation rate (14.38 mM-1 s-1) and good MRI scanning sensitivity conditions. The in vitro and in vivo results suggested the synergistic chemo-photothermal therapy of DAML/H with NIR irradiation (808 nm, 1 W cm-2, 10 min) improved the antitumor efficacy for MDR HCC. The in vivo retention experiment of Au in tumors indicated that the prepared hydrogel drug delivery system (DAML/H) had a good ability to retain Au in the tumor for a long time (at least 14 d). The western blotting results revealed that DAML/H with laser treatment could effectively downregulate P-glycoprotein (P-gp), p53 and antiapoptotic protein (Bcl-2), whereas the expression level of proapoptotic protein (Bax) and caspase-3 were increased. Therefore, DAML/H could serve as a promising synergistic chemo-photothermal therapy for MDR HCC, and a single administration might achieve long-term (14 d), on-demand, sustained-release treatment of tumors.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/tratamento farmacológico , Preparações de Ação Retardada/uso terapêutico , Doxorrubicina , Humanos , Hidrogéis/uso terapêutico , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/tratamento farmacológico , Fototerapia/métodos , Terapia Fototérmica , Nanomedicina Teranóstica
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