Baseline for plastic and hydrocarbon pollution of rivers, reefs, and sediment on beaches in Veracruz State, México, and a proposal for bioremediation.

Plastic and hydrocarbon pollution in aquatic ecosystems is a worldwide reality and serious concern today. Plastic debris presents a threat to ecosystems and organisms. Hydrocarbons are also considered priority pollutants. The hydrophobicity of the polymer in combination with the high surface area causes plastics to act as a vector for organic contaminants such as hydrocarbons. The first aim of this work was to evaluate the presence of plastic and hydrocarbon pollution in water from two reefs and two rivers and to identify plastic in six sediment beaches in Veracruz State, Mexico. In addition, the second aim was to analyse the ability of a bacterial consortium to biodegrade hydrocarbons in an airlift bioreactor and to identify degrading bacterial strains of polyethylene terephthalate (PET). Microplastics (100 nm-5 mm) were found in four water samples. Fragments of plastic collected from the reefs ranged in size from 0.716 to 32 µm and in rivers from 0.833 to 784 µm. On the sediment beaches, macroplastics of sizes 2-10 cm were detected. A number of hydrocarbons were also detected in the water samples of both reefs and one river, including n-octane, n-nonane, phenanthrene, n-eicosane, n-dotriacontane, n-hexatriacontane, n-triacontane, and n-tetratriacontane. As a biotechnological alternative for remediation of hydrocarbons and plastics, we attempted to produce a collection of native microorganisms able to degrade them. This work shows results from the bioprospection of a bacterial consortium (Xanthomonas, Acinetobacter bouvetii, Shewanella, and Aquamicrobium lusatiense) for hydrocarbon biodegradation in an airlift bioreactor. The tested consortium was able to successfully degrade the maximum diesel concentration (20 g L-1) tested for 10 days. Also, the first visual evidence of PET degradation by an isolated forest-native bacterial strain showed that Bacillus muralis is the most efficient degrader.

Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus.

Strain S658(T) was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium. The closest related species was, in both cases, Phyllobacterium trifolii PETP02(T) with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658(T) differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium, for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658(T)( = LMG 27289(T) = CECT 8230(T)).