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Biomed Environ Sci ; 34(7): 581-586, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34353424


Pathogens like bacteria and protozoa, which affect human and animal health worldwide, can be transmitted by vectors like ticks. To investigate the epidemiology and genetic diversity of bacteria and protozoans carried by ticks in Chengmai county of Hainan province, China, 285 adult hard ticks belonging to two species [ Rhipicephalus sanguineus ( sensu lato): 183, 64.21% and Rhipicephalus microplus: 102, 35.79%] from dogs, cattle, and goats were collected. Microbial families were identified in these ticks by amplifying the 18S rRNA, 16S rRNA ( rrs), citrate synthase ( gltA), and heat shock protein ( groEL) genes. Our data revealed the presence of four recognized species and two Candidatus spp. of Anaplasmataceae and Coxiellaceae. In sum, these data reveal an extensive diversity of Anaplasmataceae bacteria, Coxiellaceae bacteria, Babesiidae, and Hepatozoidae in ticks from Hainan Island, highlighting the need to understand the tick-borne pathogen infection in local animals and humans.

Anaplasmataceae/genética , Coccídios/genética , Coxiellaceae/genética , Insetos Vetores/microbiologia , Ixodidae/microbiologia , Piroplásmios/genética , Anaplasmataceae/isolamento & purificação , Animais , Chaperonina 60/genética , China , Citrato (si)-Sintase/genética , Coccídios/isolamento & purificação , Coxiellaceae/isolamento & purificação , Ilhas , Filogenia , Piroplásmios/isolamento & purificação , RNA Ribossômico 16S/genética , RNA Ribossômico 18S/genética
Vet Parasitol ; 191(3-4): 367-73, 2013 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-23017370


Canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. A reverse line blot (RLB) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including Ehrlichia canis, Hepatozoon canis and Leishmania infantum common in the Mediterranean basin. Blood samples of 110 dogs from Spain (n=21), Portugal (n=14) and Israel (n=75) were analyzed. The study evaluated 2 groups of dogs, 49 dogs with piroplasm infection detected by blood smear microscopy from Portugal, Spain and Israel, and 61 dogs surveyed from rural areas in Israel, for which infection status with vector-borne pathogens was unknown. Among the dogs previously diagnosed with piroplasmosis, infection with Babesia canis, Babesia vogeli, Babesia gibsoni and Theileria annae was detected in the Iberian dogs while only B. vogeli was found in Israeli dogs. These differences are attributed to the absence of tick vectors for some piroplasm species such as Dermacentor reticulatus in Israel. Eleven (79%) of the Babesia-positive dogs from Portugal were co-infected with other pathogens including L. infantum, H. canis and E. canis. Eight of 61 (13%) rural Israeli dogs were co-infected with two or more pathogens including B. vogeli, L. infantum, E. canis, and H. canis. Triple infections were demonstrated in 2 dogs. The RLB detection limit for Babesia was 50-fold lower than that of PCR. This study presents a RLB to simultaneously detect and separate the major vector-borne dog pathogens in southern Europe and the Middle East.

Babesia/fisiologia , Babesiose/veterinária , Coinfecção/veterinária , Doenças do Cão/diagnóstico , Doenças Parasitárias em Animais/diagnóstico , Parasitologia/métodos , Animais , Babesia/classificação , Babesiose/diagnóstico , Coinfecção/diagnóstico , Cães , Região do Mediterrâneo , Piroplásmios/fisiologia , Sensibilidade e Especificidade
Wiad Parazytol ; 57(1): 21-6, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21634230


The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.

Babesiose/diagnóstico , Babesiose/veterinária , Doenças dos Cavalos/diagnóstico , Doenças dos Cavalos/parasitologia , Piroplásmios/isolamento & purificação , Infecções Protozoárias em Animais/diagnóstico , Infecções Protozoárias em Animais/parasitologia , Animais , Babesiose/parasitologia , Cavalos , Piroplásmios/genética , Polônia , RNA de Protozoário/isolamento & purificação
Vet Parasitol ; 134(3-4): 193-213, 2005 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-16153781


A hemorrhagic disease affecting dogs in Brazil, referred to popularly as "nambiuvú" (bloody ears) and believed to be transmitted by ticks, has been observed in animals infected with an organism described originally in 1910 as a piroplasm, and known locally as Rangelia vitalii. In this series of 10 cases, the disease was characterized by anaemia, jaundice, fever, spleno- and lymphadenomegaly, hemorrhage in the gastrointestinal tract, and persistent bleeding from the nose, oral cavity and tips, margins and outer surface of the pinnae. The ixodid ticks Rhipicephalus sanguineus and Amblyomma aureolatum infested affected dogs from suburban and rural areas, respectively. Laboratory findings included regenerative anaemia, spherocytosis, icteric plasma and bilirubinuria. Those intracellular organisms were found in bone marrow smears but not in blood smears. Microscopically, zoites were seen within the cytoplasm of blood capillary endothelial cells. Parasitized and non-parasitized endothelial cells were positive immunohistochemically for von Willebrand factor (vWF). Langhans-type multinucleate giant cells were observed in the lymph nodes and choroid plexus. There was prominent erythrophagocytosis by macrophages in the lymph node sinuses and infiltration of the medullary cords by numerous plasma cells. Ultrastructurally, this organism had an apical complex that included a polar ring and rhoptries but no conoid. This parasite was contained within a parasitophorous vacuole that had a trilaminar membrane with villar protrusions and was situated in the cytoplasm of capillary endothelial cells. This organism tested positive by immunohistochemistry for Babesia microti. This pathogen was also positive by in situ hybridization for B. microti. Tentative clinical diagnosis in these cases was based on the history, clinical picture, haemogram and favorable response to therapy, and confirmed through microscopic examination of smears from the bone marrow or histological sections of multiple tissues, especially lymph nodes where zoites were most frequently found. The disease was reproduced by intravenous inoculation of blood from a naturally infected dog into an experimental dog. The authors demonstrate in this study that this organism is a protozoa of the phylum Apicomplexa, order Piroplasmorida. This piroplasm seems to be different from Babesia since it has an intraendothelial stage. Molecular phylogenetic analysis is necessary to better characterize this parasite and clarify its taxonomic status.

Babesiose/veterinária , Doenças do Cão/parasitologia , Hemorragia/veterinária , Piroplásmios/crescimento & desenvolvimento , Doenças Transmitidas por Carrapatos/veterinária , Animais , Anticorpos Antiprotozoários/análise , Babesiose/epidemiologia , Babesiose/parasitologia , Babesiose/patologia , Brasil/epidemiologia , Doenças do Cão/epidemiologia , Doenças do Cão/patologia , Cães , Feminino , Técnica Direta de Fluorescência para Anticorpo/veterinária , Hemorragia/epidemiologia , Hemorragia/parasitologia , Hemorragia/patologia , Imuno-Histoquímica/veterinária , Ixodidae/parasitologia , Rim/parasitologia , Linfonodos/parasitologia , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Piroplásmios/ultraestrutura , População Rural , População Suburbana , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/parasitologia , Doenças Transmitidas por Carrapatos/patologia
Izv Akad Nauk Ser Biol ; (3): 339-48, 2005.
Artigo em Russo | MEDLINE | ID: mdl-16004268


Vertical distribution of different groups of radiolarians and mesozooplankton (I = 0.2-3.0 mm) and the contribution of radiolarians to total biomass of bathometer-sampled zooplankton were studied in the epipelagic West Pacific Rise and the Gulf of California during 49th cruise of R/V "Akademik Mstislav Keldysh" (September-October, 2003). The production of the symbiotic algae of acantharians and colonial radiolarians as well as their contribution to the total primary production were evaluated. The proportion of all radiolarian groups in the total zooplankton biomass was shown to range from 1.4 to 11.5%. The contribution of zooxanthellae to the total primary production was not great in the studied region and equaled 0.1-1.2 and 0.3-0.7% for colonial radiolarians and acantharians, respectively.

Biomassa , Fitoplâncton/fisiologia , Piroplásmios/fisiologia , Água do Mar , Zooplâncton/fisiologia , Animais , California , Oceano Pacífico , Fitoplâncton/isolamento & purificação , Piroplásmios/isolamento & purificação , Zooplâncton/isolamento & purificação
Infect Immun ; 63(3): 811-7, 1995 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-7868251


The supernatants of in vitro cultures of Babesia divergens Rouen 1987 in human erythrocytes, obtained by using a semidefined medium based on human high-density lipoproteins, were fractionated by gel filtration chromatography into four fractions, F1 to F4. The crude supernatant as well as each fraction adjuvanted with Quil-A protected gerbils from mortality due to a homologous infectious challenge. Analysis of the humoral response of the 10 protected gerbils with fraction F4, containing major proteins with molecular masses lower than 50 kDa, showed that a few antigens (from 50 to 17 kDa) could be important candidates for an improved vaccine against B. divergens babesiosis. As an immunodominant response was directed against the 37-kDa antigen (Bd37) in two different B. divergens strains tested, a polyclonal antibody directed against Bd37 was produced in a rabbit. In an immunofluorescence assay, the anti-Bd37 antiserum strongly labelled small internal vesicles of the merozoites and the cell surface was diffusely labelled after fixation, whereas on live merozoites, this labelling was not observed. [3H]glucosamine-radiolabelling experiments demonstrate that Bd37 is a glycoprotein. The Bd37 protein can also be labelled with [14C]palmitate but not with [3H]myristic acid. In Triton X-114 temperature phase partitioning of B. divergens-infected erythrocyte extracts, Bd37 was exclusively found into the detergent phase, indicating that the palmitoylated Bd37 protein was in the membrane fraction. In the in vitro supernatant, the glycoprotein Bd37 was found in a nonpalmitoylated form, indicating excretion and/or release of the glycoprotein from the merozoite.

Antígenos de Protozoários/imunologia , Babesiose/prevenção & controle , Glicoproteínas/imunologia , Vacinas Protozoárias/imunologia , Animais , Anticorpos Antiprotozoários/biossíntese , Antígenos de Protozoários/isolamento & purificação , Babesiose/imunologia , Compartimento Celular , Feminino , Imunofluorescência , Gerbillinae , Glucosamina/metabolismo , Glicoproteínas/isolamento & purificação , Epitopos Imunodominantes , Marcação por Isótopo , Ácido Palmítico , Ácidos Palmíticos/metabolismo , Piroplásmios
Geneva; World Health Organization; 1969. (WHO/MAL/70.714).
em Inglês | WHO IRIS | ID: who-65565