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1.
Eur Rev Med Pharmacol Sci ; 26(14): 5154-5163, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35916813

RESUMO

OBJECTIVE: Partially hydrolyzed guar gum (PHGG), a water-soluble dietary fiber produced by the controlled partial enzymatic hydrolysis of guar gum beans, has various physiological roles. PHGG is expected to influence the immune function and prevent infections. The objective of this study was to examine the effect of continuous ingestion of PHGG for 12 weeks on the development of cold-like symptoms. PATIENTS AND METHODS: A placebo-controlled, double blind, randomized, parallel-group comparative study was conducted. 96 healthy Japanese adults received 5.2 g PHGG or placebo daily for 12 weeks. Cold-like symptoms were assessed based on patient diary, and the levels of short-chain fatty acids (SCFAs) in stool and blood immune markers at baseline and at weeks 6 and 12. RESULTS: The cumulative number of "no symptoms" days for all symptoms was significantly larger in the PHGG than in the placebo group. The result of the analysis by severity of cold-like symptoms also showed significant differences, with the PHGG group having a lower severity of cold-like symptoms. Propionic acid at weeks 6 and 12 and n-butyric acid and total SCFAs at week 12 were significantly higher in the PHGG than in the placebo group. The Interferon-γ level was significantly lower at week 6 in the PHGG than in the placebo group. CONCLUSIONS: PHGG intake may affect immune function and suppress cold-like symptoms through the production of SCFAs in healthy adults.


Assuntos
Galactanos , Gomas Vegetais , Adulto , Fibras na Dieta , Fezes , Humanos , Hidrólise , Mananas/uso terapêutico , Gomas Vegetais/uso terapêutico
2.
Food Chem ; 394: 133482, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35777253

RESUMO

The involvement of hydrogen bonding and hydrophobic interactions in a mixture of scallop (Patinopecten yessoensis) male gonad hydrolysates (SMGHs) and guar gum (GG) or locust-bean gum (LBG) was investigated using guanidine hydrochloride (GuHCl) and urea treatments in this study. The addition of GG and LBG (5.56 mg/mL) increased the viscosity of SMGHs at 0.1 s-1 by almost 2.5-fold and 1.7-fold, respectively, reaching 254.8 and 177.0 Pa·s. After treatment with GuHCl or urea, the mixed gels (SMGHs/GG and SMGHs/LBG) became relatively transparent and more fluid, as the viscosity significantly reduced. Moreover, changes of moisture distribution and conformational characteristics suggested that hydrogen bonding and hydrophobic interactions were the main intermolecular forces in the mixed gels of SMGHs and GG or LBG. Furthermore, the SMGHs/GG and SMGHs/LBG mixtures yielded strong gels with viscous network structures, indicating that these materials can be used as thickening agents in food systems.


Assuntos
Pectinidae , Gomas Vegetais , Animais , Galactanos/química , Géis/química , Gônadas/química , Masculino , Mananas/química , Pectinidae/química , Gomas Vegetais/química , Polissacarídeos/análise , Ureia/análise
3.
PLoS One ; 17(7): e0271623, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35901085

RESUMO

Currently, hydrogels are considered as ideal biomaterials due to their unique structure and characteristics that facilitates considerable hydrophilicity, swelling, drug loading and release. In this study, we report pH-responsive GG-MAA-AMPS hydrogel delivery system prepared via free radical polymerization technique. Hydrogels were loaded with Metformin HCl as a model drug. Hydrogels were characterized through Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM). FTIR confirmed the successful crosslinking of reactants, hydrogel network formation and drug loading. TGA and DSC proved the higher thermal stability of reactants after crosslinking and drug loading. XRD analysis showed decrease in crystallinity of drug after loading into the hydrogels. SEM revealed smooth and glassy appearance of both loaded and unloaded hydrogels. Gel content was increased with increase in concentration of reactants. Drug entrapment was decreased by increasing concentration of GG and AMPS while MAA acted inversely. Hydrogels displayed pH-dependent swelling and drug release behavior being high at pH 6.8 and 7.4 while low at acidic pH (1.2). Oral tolerability in rabbits showed that hydrogels were safe without causing any hematological or histopathological changes in healthy rabbits. Based on the obtained results, GG-MAA-AMPS can be considered as potential carrier for metformin HCl as well as other hydrophilic drugs.


Assuntos
Metformina , Animais , Preparações de Ação Retardada , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Galactanos , Hidrogéis/química , Concentração de Íons de Hidrogênio , Mananas , Microscopia Eletrônica de Varredura , Gomas Vegetais , Polímeros , Coelhos , Espectroscopia de Infravermelho com Transformada de Fourier
4.
Int J Biol Macromol ; 215: 579-595, 2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-35779651

RESUMO

The biopolymers-based two-fold system could provide a sustained release platform for drug delivery to the brain resisting the mucociliary clearance, enzymatic degradation, bypassing the first-pass hepatic metabolism, and BBB thus providing superior bioavailability through intranasal administration. In this study, poloxamers PF-127/PF-68 grafted chitosan HCl-co-guar gum-based thermoresponsive hydrogel loaded with eletriptan hydrobromide laden pullulan nanoparticles was synthesized and subjected to dynamic light scattering, Fourier transform infrared spectroscopy, thermal analysis, x-ray diffraction, scanning electron microscopy, stability studies, mucoadhesive strength and time, gel strength, cloud point assessment, rheological assessment, ex-vivo permeation, cell viability assay, histology studies, and in-vivo Pharmacokinetics studies, etc. It is quite evident that CSG-EH-NPs T-Hgel has an enhanced sustained release drug profile where approximately 86 % and 84 % of drug released in phosphate buffer saline and simulated nasal fluid respectively throughout 48 h compared to EH-NPs where 99.44 % and 97.53 % of the drug was released in PBS and SNF for 8 h. In-vivo PKa parameters i.e., mean residence time (MRT) of 11.9 ± 0.83 compared to EH-NPs MRT of 10.2 ± 0.92 and area under the curve (AUCtot) of 42,540.5 ± 5314.14 comparing to AUCtot of EH-NPs 38,026 ± 6343.1 also establish the superiority of CSG-EH-NPs T-Hgel.


Assuntos
Quitosana , Nanopartículas , Encéfalo/metabolismo , Quitosana/química , Preparações de Ação Retardada , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Galactanos , Glucanos , Hidrogéis/química , Mananas , Nanopartículas/química , Gomas Vegetais
5.
Int J Mol Sci ; 23(13)2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35806443

RESUMO

Surface coatings of materials by polysaccharide polymers are an acknowledged strategy to modulate interfacial biocompatibility. Polysaccharides from various algal species represent an attractive source of structurally diverse compounds that have found application in the biomedical field. Furcellaran obtained from the red algae Furcellaria lumbricalis is a potential candidate for biomedical applications due to its gelation properties and mechanical strength. In the present study, immobilization of furcellaran onto polyethylene terephthalate surfaces by a multistep approach was studied. In this approach, N-allylmethylamine was grafted onto a functionalized polyethylene terephthalate (PET) surface via air plasma treatment. Furcellaran, as a bioactive agent, was anchored on such substrates. Surface characteristics were measured by means of contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Subsequently, samples were subjected to selected cell interaction assays, such as antibacterial activity, anticoagulant activity, fibroblasts and stem cell cytocompatibility, to investigate the Furcellaran potential in biomedical applications. Based on these results, furcellaran-coated PET films showed significantly improved embryonic stem cell (ESC) proliferation compared to the initial untreated material.


Assuntos
Alginatos , Polietilenotereftalatos , Antibacterianos/farmacologia , Gomas Vegetais , Polietilenotereftalatos/química , Polímeros/química , Propriedades de Superfície
6.
ACS Appl Mater Interfaces ; 14(27): 31343-31353, 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35786849

RESUMO

In this work, the guar gum (GG) and the electrospinned ethyl cellulose-polyvinyl pyrrolidone (EC-PVP) nanofibers were used as humidity-sensitive materials for fabricating a quartz crystal microbalance (QCM) sensor. Fourier transform infrared spectroscopy, scanning electron microscopy, water contact angle test, and X-ray photoelectron spectra were used to characterize the synthesized GG/EC-PVP composite material, confirming its successful preparation and good hydrophilicity. The humidity sensitivity experiments were performed at room temperature. The GG/EC-PVP-coated QCM sensor has high sensitivity (55.72 Hz/%RH) and low hysteresis (2.8% RH) in a wide relative humidity range (0-97% RH), short response/recovery time (26/2 s), excellent selectivity, good repeatability, and stability. The combined action of hydrophilic groups and porous structure enhances the humidity sensitivity. The GG/EC-PVP sensor can be used to capture and measure typical breathing patterns in different human basic emotions due to its good performance. Furthermore, a lie-detector system was also designed for judging the lying through detecting the emotional breathing pattern of the subjects.


Assuntos
Polivinil , Técnicas de Microbalança de Cristal de Quartzo , Celulose/análogos & derivados , Galactanos , Humanos , Umidade , Mananas , Gomas Vegetais , Povidona , Técnicas de Microbalança de Cristal de Quartzo/métodos , Respiração
7.
Mol Ecol ; 31(15): 4095-4111, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35691023

RESUMO

Intense research efforts over the last two decades have renewed our understanding of plant phylogeography and domestication in the Mediterranean basin. Here we aim to investigate the evolutionary history and the origin of domestication of the carob tree (Ceratonia siliqua), which has been cultivated for millennia for food and fodder. We used >1000 microsatellite genotypes to delimit seven carob evolutionary units (CEUs). We investigated genome-wide diversity and evolutionary patterns of the CEUs with 3557 single nucleotide polymorphisms generated by restriction-site associated DNA sequencing (RADseq). To address the complex wild vs. cultivated status of sampled trees, we classified 56 sampled populations across the Mediterranean basin as wild, seminatural or cultivated. Nuclear and cytoplasmic loci were identified from RADseq data and separated for analyses. Phylogenetic analyses of these genomic-wide data allowed us to resolve west-to-east expansions from a single long-term refugium probably located in the foothills of the High Atlas Mountains near the Atlantic coast. Our findings support multiple origins of domestication with a low impact on the genetic diversity at range-wide level. The carob was mostly domesticated from locally selected wild genotypes and scattered long-distance westward dispersals of domesticated varieties by humans, concomitant with major historical migrations by Romans, Greeks and Arabs. Ex situ efforts to preserve carob genetic resources should prioritize accessions from both western and eastern populations, with emphasis on the most differentiated CEUs situated in southwest Morocco, south Spain and eastern Mediterranean. Our study highlights the relevance of wild and seminatural habitats in the conservation of genetic resources for cultivated trees.


Assuntos
Domesticação , Fabaceae , Filogenia , Fabaceae/genética , Frutas , Galactanos , Variação Genética , Mananas , Região do Mediterrâneo , Gomas Vegetais
8.
Molecules ; 27(12)2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35744846

RESUMO

Plant gums are bio-organic substances that are derived from the barks of trees. They are biodegradable and non-adverse complex polysaccharides that have been gaining usage in recent years due to a number of advantages they contribute to various applications. In this study, gum was collected from Moringa oleifera and Azadirachta indica trees, then dried and powdered. Characterizations of gum polysaccharides were performed using TLC, GC-MS, NMR, etc., and sugar molecules such as glucose and xylose were found to be present. Effects of the gums on Abelmoschus esculentus growth were observed through root growth, shoot growth, and biomass content. The exposure of the seeds to the plant gums led to bio stimulation in the growth of the plants. Poor quality soil was exposed to the gum polysaccharide, where the polysaccharide was found to improve soil quality, which was observed through soil analysis and SEM analysis of soil porosity and structure. Furthermore, the plant gums were also found to have bio-pesticidal activity against mealybugs, which showed certain interstitial damage evident through histopathological analysis.


Assuntos
Azadirachta , Moringa oleifera , Praguicidas , Moringa oleifera/química , Gomas Vegetais/química , Gomas Vegetais/farmacologia , Plantas , Polissacarídeos/química , Polissacarídeos/farmacologia , Solo
9.
Food Chem ; 393: 133422, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-35689924

RESUMO

The effects of guar gum (GG), xanthan gum (XG), carrageenan gum (CG), xanthan-guar gum blend (XG-GG), chitosan (CS), gum arabic (GA) on the water migration, rheological and 3D printing properties of ß-carotene loaded yam starch-based hydrogel (BCH) were investigated to expand product form of ß-carotene. The results showed that CS addition promoted the migration of weakly bound water to tightly bound water in BCH. Addition of GG, CG, XG-GG, CS and GA enhanced apparent viscosity, G', G'', hardness and gumminess of BCH. CG, XG-GG, CS and GA addition improved printing stability of BCH. The printed objects added with GG and CS displayed smooth lines with fine resolution and higher formability, which showed a more uniform pore distribution and thinner gel skeleton structure. The results of XRD showed that hydrocolloids addition decreased the relative crystallinity of BCH. A combination of physicochemical parameters could be used to discriminate samples through hierarchical cluster analysis.


Assuntos
Dioscorea , Amido , Carragenina , Coloides/química , Goma Arábica/química , Hidrogéis , Gomas Vegetais/química , Polissacarídeos Bacterianos/química , Impressão Tridimensional , Reologia , Amido/química , Viscosidade , Água/química , beta Caroteno
10.
Food Funct ; 13(12): 6674-6687, 2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35647651

RESUMO

Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism. In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats. After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L-1) as compared with that of the model group (14.2 mmol L-1), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%. Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS). CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level. Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria, while that of Shigella was decreased. The molecular mechanisms involved in the anti-diabetic effects of CMOS were further studied. CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats. Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1ß secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation. Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver. Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.


Assuntos
Cassia , Diabetes Mellitus Experimental , Oligossacarídeos , Animais , Glicemia/metabolismo , Cassia/química , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Glucose/metabolismo , Inflamação/tratamento farmacológico , Insulina , Oligossacarídeos/farmacologia , Gomas Vegetais/química , RNA Mensageiro , Ratos , Sementes/química , Estreptozocina
11.
Int J Biol Macromol ; 209(Pt B): 2197-2212, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35508229

RESUMO

Diverse properties of natural gums have made them quite useful for various pharmaceutical applications. However, they suffer from various problems, including unregulated hydration rates, microbial degradation, and decline in viscosity during warehousing. Among various chemical procedures for modification of gums, carboxymethylation has been widely studied due to its simplicity and efficiency. Despite the availability of numerous research articles on natural gums and their uses, a comprehensive review on carboxymethylation of natural gums and their applications in the pharmaceutical and other biomedical fields is not published until now. This review outlines the classification of gums and their derivatization methods. Further, we have discussed various techniques of carboxymethylation, process of determination of degree of substitution, and functionalization pattern of substituted gums. Detailed information about the application of carboxymethyl gums as drug delivery carriers has been described. The article also gives a brief account on tissue engineering and cell delivery potential of carboxymethylated gums.


Assuntos
Portadores de Fármacos , Excipientes , Fenômenos Químicos , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Excipientes/química , Gomas Vegetais/química , Viscosidade
12.
Food Funct ; 13(11): 6022-6035, 2022 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-35611754

RESUMO

There is a growing interest in designing novel food microstructures that can control nutrient digestion and provide satiety for tackling obesity. In this study, phase separated microstructures of skimmed milk powder (SMP) and guar gum (GG) were the main focus, and these can be considered as water-in-water (W/W) emulsions. Through the incorporation of oil into these systems, it was possible to form model systems of SMP-GG-OIL, showing the lipid phase within the protein phase within the polysaccharide phase. The in vitro digestibility of such phase separated model systems of SMP-GG-OIL with different microstructures was investigated using a pH stat method. Confocal laser scanning microscopy also revealed structural changes that occurred to the emulsified lipid droplets as they passed through a gastrointestinal (GI) model. The microstructures were created based on the tie-lines on a previously established phase diagram of SMP-GG, and shown to be able to control lipid digestion. For a selected tie-line, the lipolysis follows the order: protein continuous > bi-continuous > polysaccharide continuous system, at a certain level of oil addition. The mechanism involved in the lipolysis of the designed formulations/microstructures was dependent upon the protein, rather than GG, and was driven by the protein concentration. These findings provide insights for potential applications in functional food designing in the food industry.


Assuntos
Digestão , Proteínas do Leite , Emulsões/química , Galactanos , Lipídeos/química , Mananas , Tamanho da Partícula , Gomas Vegetais , Polissacarídeos , Água/química
13.
Curr Opin Otolaryngol Head Neck Surg ; 30(3): 169-176, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35635111

RESUMO

PURPOSE OF REVIEW: Thickened fluids are a widely utilised compensatory management strategy for people with impaired swallowing (dysphagia). Over recent years there has been a shift in practice to offer gum-based instead of starch-based products. A key marketing message has been that gum-based thickeners with amylase-resistant properties are superior in promoting 'safer swallowing'. This review sought evidence to evaluate the effect of amylase-resistant products on swallowing safety. RECENT FINDINGS: No studies directly compared the effect of amylase-resistant products with usual care or products without amylase resistance. Five studies cited amylase-resistant properties and compared gum-based to starch-based dysphagia products or thin fluids. Swallowing safety was frequently judged subjectively with rating scales. Swallowing biomechanics were not included and clinically meaningful outcomes, such as incidence of aspiration pneumonia, were not reported. A scoping review of the grey literature found little evidence that amylase-resistant properties of dysphagia products were of significant concern to clinicians or patients. SUMMARY: Despite references to the 'importance' of amylase-resistant properties of dysphagia products there is no evidence that this property improves swallowing safety. Further research is needed using objective and clinically meaningful outcome measures to allow clinicians and patients to make informed decisions for dysphagia management.


Assuntos
Transtornos de Deglutição , Deglutição , Amilases , Bebidas , Transtornos de Deglutição/etiologia , Transtornos de Deglutição/terapia , Aditivos Alimentares , Humanos , Gomas Vegetais , Pneumonia Aspirativa/prevenção & controle , Amido
14.
Int J Biol Macromol ; 211: 116-127, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35561853

RESUMO

The current work demonstrates a unique approach of utilizing nanochitosan (NCS) based edible nanomodifier for functionalizing starch (ST)/guar gum (GG) biocomposite with superior packaging properties targeting stringent edible food packaging on fresh cuts. The effectiveness of NCS in terms of structure-property-performance analysis of ST/GG biocomposites was done. The inclusion of NCS to the biocomposites of ST/GG converts its hydrophilic surface nature to hydrophobic (contact angle of ~114°) by modifying the surface features. The addition of NCS improved the thermal stability, where the observed 10% weight degradation of ST biocomposites were ~79.36, ~80.49, and ~186.89 °C for neat ST, ST/GG biocomposites, and ST/GG/NCS (3% w/v) (ST-GG-NCS3), respectively. The observed transparency of ST, ST-GG, and ST-GG-NCS3 were 21, 8, and 48%, respectively in the visible region suggesting consumer preference for transparent packaging materials. The wt% of O, C and N elements in ST-GG-NCS3 as observed by EDX spectra were ~ 50.2, ~47.6, and ~ 2.2%, respectively, which confirm the safety of the materials. Additionally, it is noteworthy to mention that the storage quality in terms of microbial growth, pH change, color attributes, and weight loss are better preserved when used as an edible coating on cut apple fruits.


Assuntos
Filmes Comestíveis , Galactanos/química , Mananas , Gomas Vegetais , Amido/química , Propriedades de Superfície
15.
Int J Biol Macromol ; 211: 238-248, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35561863

RESUMO

Active/intelligent packaging films were developed by incorporating purple sweetpotato anthocyanins (PSA) and purple cabbage anthocyanins (PCA) in locust bean gum/polyvinyl alcohol (LP), chitosan/polyvinyl alcohol (CP) and κ-carrageenan/polyvinyl alcohol (KP) film matrices. The joint effect of anthocyanins' source and polysaccharides' nature on the structure and functionality of the films was determined. Results showed PSA and PCA interacted with film matrices through hydrogen bonds and/or electrostatic interactions, resulting in improved film uniformity. PSA and PCA did not remarkably alter the water vapor permeability and tensile strength of the films; however, significantly improved the light barrier ability, antioxidant activity, pH-sensitivity and ammonia-sensitivity of films. For the films containing the same anthocyanins (PSA or PCA), LP-based films had relatively higher light barrier ability and storage stability, while CP-based had relatively weaker color changeable ability. When PSA and PCA were immobilized in the same film matrix, the films containing PCA had higher light barrier ability and antioxidant activity than the films containing PSA. Among the films, LP-PCA film exhibited a good potential to monitor the freshness of shrimp. Results suggested the structure and functionality of the films were influenced by the source of anthocyanins and the nature of polysaccharides.


Assuntos
Brassica , Quitosana , Ipomoea batatas , Antocianinas/química , Antioxidantes/química , Antioxidantes/farmacologia , Carragenina , Quitosana/química , Embalagem de Alimentos/métodos , Galactanos , Concentração de Íons de Hidrogênio , Mananas , Gomas Vegetais , Álcool de Polivinil/química
16.
Int J Biol Macromol ; 211: 57-73, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35576698

RESUMO

Nosocomial bacterial infections associated with biofilms inspire to explore newer bactericidal strategy with eco-friendly biomaterials as sustainable alternatives. In this research work, we successfully developed bio-safe films from kojic acid(KA) and guar gum propionate(GGP) for Escherichia coli biofilm disruption and planktonic cell killing. High DS(degree of substitution = 1.52) GGP was synthesized from guar gum (GG)assisted by chaotropic ions at room-temperature. Biopolymers were routinely characterized in CHN analyzer, FT-IR, TGA and XRD analysis. KA loaded GGP films were prepared by cross-linking the molecules in presence of epichlorhydrin and two different percentages of KA were employed. Film physical and tensile properties were systematically evaluated and optimized. Water vapour permeability (WVP) and tensile strength of final film GGPFK10 were recorded at 0.741 ± 0.09gmm-1kPa-1h-1 and 19.23 MPa. KA release from GGP matrix followed controlled diffusion process. MIC of GGP was 130 µg/mL and zone of inhibition of GGPFK10 was confirmed at 16.1 mm. SEM experiments disclosed the absence of pili-like structures with squeezed and elongated cellular morphology in dead planktonic cells. Disruption of biofilms was experimented in detail by CV assay, fluorescent, light microscopic and SEM studies. The film showed excellent cell-viability on human adult dermal fibroblast (HADF)cell-line. Overall, the biosafe film would be a potent antibacterial device for treating infections against E.coli biofilms and planktonic cells.


Assuntos
Escherichia coli , Propionatos , Adulto , Antibacterianos/química , Antibacterianos/farmacologia , Biofilmes , Galactanos/química , Galactanos/farmacologia , Humanos , Mananas/química , Mananas/farmacologia , Plâncton , Gomas Vegetais/química , Gomas Vegetais/farmacologia , Pironas , Espectroscopia de Infravermelho com Transformada de Fourier
17.
Nutrients ; 14(7)2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35406064

RESUMO

Carob is one of the major food trees for peoples of the Mediterranean basin, but it has also been traditionally used for medicinal purposes. Carob contains many nutrients and active natural products, and D-Pinitol is clearly one of the most important of these. D-Pinitol has been reported in dozens of scientific publications and its very diverse medicinal properties are still being studied. Presently, more than thirty medicinal activities of D-Pinitol have been reported. Among these, many publications have reported the strong activities of D-Pinitol as a natural antidiabetic and insulin regulator, but also as an active anti-Alzheimer, anticancer, antioxidant, and anti-inflammatory, and is also immune- and hepato-protective. In this review, we will present a brief introduction of the nutritional and medicinal importance of Carob, both traditionally and as found by modern research. In the introduction, we will present Carob's major active natural products. The structures of inositols will be presented with a brief literature summary of their medicinal activities, with special attention to those inositols in Carob, as well as D-Pinitol's chemical structure and its medicinal and other properties. D-Pinitol antidiabetic and insulin regulation activities will be extensively presented, including its proposed mechanism of action. Finally, a discussion followed by the conclusions and future vision will summarize this article.


Assuntos
Produtos Biológicos , Fabaceae , Produtos Biológicos/farmacologia , Fabaceae/química , Galactanos , Hipoglicemiantes/farmacologia , Inositol/análogos & derivados , Inositol/farmacologia , Insulina , Mananas , Gomas Vegetais
18.
Int J Biol Macromol ; 209(Pt A): 1307-1318, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35483509

RESUMO

The aim of this work was to develop active packaging film by using chitosan/guar gum (CG) film matrix and walnut green husk extract (WE), for preservation of fresh-cut apple. WE was used as cross-linking agent to improve physicochemical properties, and as active substances to enhance antioxidant activity of CG films. Fourier transform infrared spectroscopy and scanning electron microscopy results showed WE formed intermolecular hydrogen bond interactions with the film matrix, and microstructures of the film were more compact. With the increase of WE content (0-4 wt%), the mechanical properties of composite films were significantly enhanced, while permeability of water vapor and oxygen was significantly decreased (p < 0.05). When the amount of extract reached 4 wt%, the DPPH radical scavenging activity of composite film was significantly increased to 94.59%. CG-WE and CG films were used as active packaging materials to preserve fresh-cut apple. When stored at 4 °C for 10 days, CG-WE films showed better performance in reducing firmness, weight loss, total soluble solids and inhibiting browning and microbial growth of fresh-cut apples. Therefore, as a new type of active food packaging material, CG-WE films have good physical properties, and great potential in ensuring food quality and extending shelf life.


Assuntos
Quitosana , Juglans , Malus , Quitosana/química , Embalagem de Alimentos , Galactanos , Mananas , Permeabilidade , Extratos Vegetais , Gomas Vegetais
19.
Food Chem ; 386: 132810, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-35364496

RESUMO

Molecular structure and functional properties of glycinin conjugated to κ-carrageenan and guar gum using a dry-heating method were comparatively analyzed. Glycosylation was confirmed by analyzing the degree of grafting, protein subunit composition, infrared absorption profile, and changes in contents of protein secondary structures. K-carrageenan was proven to possess a greater susceptibility to be grafted to glycinin than guar gum due to its relatively low molecular weight and negatively charged characteristics. The improvement of solubility by glycosylation with guar gum near the isoelectric point of glycinin was better than that by glycosylation with κ-carrageenan. Glycinin glycosylated with both polysaccharides exhibited enhanced emulsifying activity and stability. The enhanced apparent viscosity, elastic modulus, and viscous modulus also demonstrated that glycosylation promoted the appearance of stable elastic network structure. In summary, glycosylation with these two polysaccharides conferred glycinin superior emulsifying and rheological properties, and κ-carrageenan exhibited a better performance compared to guar gum.


Assuntos
Polissacarídeos , Carragenina/química , Galactanos , Globulinas , Mananas , Estrutura Molecular , Gomas Vegetais , Polissacarídeos/química , Proteínas de Soja
20.
J Food Sci ; 87(5): 2058-2071, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35411576

RESUMO

Influence of different concentrations (0.5, 1.0, 2.0, and 3.0% w/v) and temperatures (4, 25, 50, and 75°C) on particle size distribution (PSD) and rheological and tribological characteristics of flaxseed gum (FSG) solutions was investigated. Besides, FSG dispersions (0.5, 1.0, and 2.0% w/v) were used as edible coating and their influence on the quality parameters (oil uptake, moisture loss, texture, and sensory properties) of fried potato chips was studied. All FSG dispersions revealed shear-thinning nature and viscous properties (as G″ > G') that were more dominant at higher concentrations and lower temperatures. The power-law model presented a good fit in demonstrating the flow behavior of FSG dispersions. Concentration was the variable that affected the tribology of FSG dispersions, while temperature had little effect on the tribology. Particle size distribution was increased with the increasing concentration of FSG. FSG dispersions as an edible coating effectively reduced the moisture loss, oil uptake, and hardness properties of potato chips. Practical Application Profiling the influence of concentration and temperature on the rheology and tribology of flaxseed gum is particularly valuable during food processing. The results predict the physical properties of coated potato chips that can support the potential application of flaxseed gum as a coating agent. Today's consumers prefer healthier food products with low caloric, higher fiber content, functional properties, and sensory qualities. Food industries can use FSG as a low-cost natural coating material in terms of economic benefits, consumer acceptance, and providing an inordinate potential both for its protective effect and carrying functional compounds such as antioxidants in their coating matrix.


Assuntos
Linho , Gomas Vegetais , Reologia , Temperatura , Viscosidade
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