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1.
Skin Res Technol ; 30(7): e13850, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38979986

RESUMO

BACKGROUND: Current treatment options for Malassezia folliculitis (MF) are limited. Recent research has demonstrated the inhibitory effect of cold atmospheric plasma (CAP) on the growth of Malassezia pachydermatis in vitro, suggesting CAP as a potential therapeutic approach for managing MF. OBJECTIVES: The objective of our study is to assess the in vitro antifungal susceptibility of Malassezia yeasts to CAP. Additionally, we aim to evaluate the efficacy and tolerability of CAP in treating patients with MF. METHODS: We initially studied the antifungal effect of CAP on planktonic and biofilm forms of Malassezia yeasts, using well-established techniques such as zone of inhibition, transmission electron microscopy, colony count assay and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt assay. Subsequently, a randomized (1:1 ratio), active comparator-controlled, observer-blind study was conducted comparing daily CAP therapy versus itraconazole 200 mg/day for 2 weeks in 50 patients with MF. Efficacy outcomes were measured by success rate, negative microscopy rate and changes in Dermatology Life Quality Index (DLQI) and Global Aesthetic Improvement Scale (GAIS) scores. Safety was assessed by monitoring adverse events (AEs) and local tolerability. RESULTS: In laboratory investigations, CAP time-dependently inhibited the growth of Malassezia yeasts in both planktonic and biofilm forms. Forty-nine patients completed the clinical study. At week 2, success was achieved by 40.0% of subjects in the CAP group versus 58.3% in the itraconazole group (p = 0.199). The negative direct microscopy rates of follicular samples were 56.0% in the CAP group versus 66.7% in the itraconazole group (p = 0.444). No significant differences were found in the proportion of subjects achieving DLQI scores of 0/1 (p = 0.456) or in the GAIS responder rates (p = 0.588) between the two groups. Three patients in the CAP group and one patient in the itraconazole group reported mild AEs. CONCLUSION: CAP demonstrated significant antifungal activity against Malassezia yeasts in vitro and exhibited comparable efficacy to itraconazole in treating MF patients. Without the associated adverse effects of oral antifungal drugs, CAP can be considered a promising and safe treatment modality for MF.


Assuntos
Antifúngicos , Dermatomicoses , Foliculite , Malassezia , Gases em Plasma , Malassezia/efeitos dos fármacos , Humanos , Foliculite/tratamento farmacológico , Foliculite/microbiologia , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Adulto , Feminino , Masculino , Pessoa de Meia-Idade , Dermatomicoses/tratamento farmacológico , Dermatomicoses/microbiologia , Itraconazol/uso terapêutico , Itraconazol/farmacologia , Adulto Jovem , Resultado do Tratamento , Biofilmes/efeitos dos fármacos
2.
Int J Mol Sci ; 25(13)2024 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-39000064

RESUMO

Chondrosarcoma (CS) is a rare malignant bone sarcoma that primarily affects cartilage cells in the femur and pelvis. While most subtypes exhibit slow growth with a very good prognosis, some aggressive subtypes have a poorer overall survival. CS is known for its resistance to chemotherapy and radiotherapy, leaving surgery as the sole effective therapeutic option. Cold physical plasma (CPP) has been explored in vitro as a potential therapy, demonstrating positive anti-tumor effects on CS cells. This study investigated the synergistic effects of combining CPP with cytostatics on CS cells. The chemotherapeutic agents cisplatin, doxorubicin, and vincristine were applied to two CS cell lines (CAL-78 and SW1353). After determining their IC20 and IC50, they were combined with CPP in both cell lines to assess their impact on the cell proliferation, viability, metabolism, and apoptosis. This combined approach significantly reduced the cell proliferation and viability while increasing the apoptosis signals compared to cytostatic therapy alone. The combination of CPP and chemotherapeutic drugs shows promise in targeting chemoresistant CS cells, potentially improving the prognosis for patients in clinical settings.


Assuntos
Apoptose , Neoplasias Ósseas , Proliferação de Células , Sobrevivência Celular , Condrossarcoma , Doxorrubicina , Gases em Plasma , Condrossarcoma/tratamento farmacológico , Condrossarcoma/patologia , Humanos , Gases em Plasma/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Doxorrubicina/farmacologia , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Neoplasias Ósseas/tratamento farmacológico , Neoplasias Ósseas/patologia , Neoplasias Ósseas/terapia , Antineoplásicos/farmacologia , Cisplatino/farmacologia , Vincristina/farmacologia , Terapia Combinada
3.
J Med Microbiol ; 73(7)2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38985505

RESUMO

Introduction. Aspergillus flavus and Fusarium keratoplasticum are common causative pathogens of fungal keratitis (FK), a severe corneal disease associated with significant morbidity and vision loss. Escalating incidence of antifungal resistance to available antifungal drugs poses a major challenge to FK treatment. Cold atmospheric plasma (CAP) is a pioneering nonpharmacologic antimicrobial intervention that has demonstrated potential as a broad-spectrum antifungal treatment.Gap statement. Previous research highlights biofilm-associated resistance as a critical barrier to effective FK treatment. Although CAP has shown promise against various fungal infections, its efficacy against biofilm and conidial forms of FK pathogens remains inadequately explored.Aim. This study aims to investigate the antifungal efficacy of CAP against clinical fungal keratitis isolates of A. flavus and F. keratoplasticum in vitro.Methodology. Power parameters (22-27 kVpp, 300-400 Hz and 20-80 mA) of a dielectric barrier discharge CAP device were optimized for inactivation of A. flavus biofilms. Optimal applied voltage and total current were applied to F. keratoplasticum biofilms and conidial suspensions of A. flavus and F. keratoplasticum. The antifungal effect of CAP treatment was investigated by evaluating fungal viability through means of metabolic activity, c.f.u. enumeration (c.f.u. ml-1) and biofilm formation.Results. For both fungal species, CAP exhibited strong time-dependent inactivation, achieving greater than 80 % reduction in metabolic activity and c.f.u. ml-1 within 300 s or less, and complete inhibition after 600 s of treatment.Conclusion. Our findings indicate that CAP is a promising broad-spectrum antifungal intervention. CAP treatment effectively reduces fungal viability in both biofilm and conidial suspension cultures of A. flavus and F. keratoplasticum, suggesting its potential as an alternative treatment strategy for fungal keratitis.


Assuntos
Antifúngicos , Aspergillus flavus , Biofilmes , Fusarium , Ceratite , Gases em Plasma , Esporos Fúngicos , Aspergillus flavus/efeitos dos fármacos , Fusarium/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Gases em Plasma/farmacologia , Esporos Fúngicos/efeitos dos fármacos , Antifúngicos/farmacologia , Ceratite/microbiologia , Infecções Oculares Fúngicas/microbiologia , Humanos , Fusariose/microbiologia , Viabilidade Microbiana/efeitos dos fármacos
4.
Sci Rep ; 14(1): 15930, 2024 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-38987305

RESUMO

The paper reports a low-cost handheld source of a cold air plasma intended for biomedical applications that can be made by anyone (detailed technical information and a step-by-step guide for creating the NTP source are provided). The plasma source employs a 1.4 W corona discharge in the needle-to-cone electrode configuration and is an extremely simple device, consisting basically of two electrodes and a cheap power supply. To achieve the best bactericidal effect, the plasma source has been optimized on Escherichia coli. The bactericidal ability of the plasma source was further tested on a wide range of microorganisms: Staphylococcus aureus as a representative of gram-positive bacteria, Pseudomonas aeruginosa as gram-negative bacteria, Candida albicans as yeasts, Trichophyton interdigitale as microfungi, and Deinococcus radiodurans as a representative of extremophilic bacteria resistant to many DNA-damaging agents, including ultraviolet and ionizing radiation. The testing showed that the plasma source inactivates all the microorganisms tested in several minutes (up to 105-107 CFU depending on a microorganism), proving its effectiveness against a wide spectrum of pathogens, in particular microfungi, yeasts, gram-positive and gram-negative bacteria. Studies of long-lived reactive species such as ozone, nitrogen oxides, hydrogen peroxide, nitrite, and nitrate revealed a strong correlation between ozone and the bactericidal effect, indicating that the bactericidal effect should generally be attributed to reactive oxygen species. This is the first comprehensive study of the bactericidal effect of a corona discharge in air and the formation of long-lived reactive species by the discharge, depending on both the interelectrode distance and the discharge current.


Assuntos
Gases em Plasma , Gases em Plasma/farmacologia , Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Candida albicans/efeitos dos fármacos
5.
J Med Microbiol ; 73(7)2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38967406

RESUMO

Introduction. Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as plasma medicine for treating various diseases, including infections caused by Candida albicans, an opportunistic pathogen that can overgrow in individuals with weakened immune system.Gap Statement. To date, there has been less molecular study on cold plasma-treated C. albicans.Research Aim. The study aims to fill the gap in understanding the molecular response of C. albicans to cold plasma treatment.Methodology. This project involved testing a cold plasma generator to determine its antimicrobial effectiveness on C. albicans' planktonic cells. Additionally, the cells' transcriptomics responses were investigated using RNA sequencing at various treatment durations (1, 3 and 5 min).Results. The results show that our cold plasma effectively eliminates C. albicans. Cold plasma treatment resulted in substantial downregulation of important pathways, such as 'nucleotide metabolism', 'DNA replication and repair', 'cell growth', 'carbohydrate metabolism' and 'amino acid metabolism'. This was an indication of cell cycle arrest of C. albicans to preserve energy consumption under unfavourable conditions. Nevertheless, C. albicans adapted its GSH antioxidant system to cope with the oxidative stress induced by reactive oxygen species, reactive nitrogen species and other free radicals. The treatment likely led to a decrease in cell pathogenicity as many virulence factors were downregulated.Conclusion. The study demonstrated the major affected pathways in cold plasma-treated C. albicans, providing valuable insights into the molecular response of C. albicans to cold plasma treatment. The findings contribute to the understanding of the antimicrobial efficiency of cold plasma and its potential applications in the field of microbiology.


Assuntos
Candida albicans , Perfilação da Expressão Gênica , Gases em Plasma , Candida albicans/genética , Candida albicans/efeitos dos fármacos , Gases em Plasma/farmacologia , Plâncton/genética , Transcriptoma , Estresse Oxidativo , Regulação Fúngica da Expressão Gênica , Espécies Reativas de Oxigênio/metabolismo , Humanos
6.
Environ Res ; 257: 119333, 2024 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-38849000

RESUMO

This review is aimed at researchers in air pollution control seeking to understand the latest advancements in volatile organic compound (VOC) removal. Implementing of plasma-catalysis technology for the removal of volatile organic compounds (VOCs) led to a significant boost in terms of degradation yield and mineralization rate with low by-product formation. The plasma-catalysis combination can be used in two distinct ways: (I) the catalyst is positioned downstream of the plasma discharge, known as the "post plasma catalysis configuration" (PPC), and (II) the catalyst is located in the plasma zone and exposed directly to the discharge, called "in plasma catalysis configuration" (IPC). Coupling these two technologies, especially for VOCs elimination has attracted the interest of many researchers in recent years. The term "synergy" is widely reported in their works and associated with the positive effect of the plasma catalysis combination. This review paper investigates the state of the art of newly published papers about catalysis, photocatalysis, non-thermal plasma, and their combination for VOC removal application. The focus is on understanding different synergy sources operating mutually between plasma and catalysis discussed and classified into two main parts: the effect of the plasma discharge on the catalyst and the effect of the catalyst on plasma discharge. This approach has the potential for application in air purification systems for industrial processes or indoor environments.


Assuntos
Poluentes Atmosféricos , Gases em Plasma , Compostos Orgânicos Voláteis , Compostos Orgânicos Voláteis/química , Catálise , Gases em Plasma/química , Poluentes Atmosféricos/química , Poluentes Atmosféricos/análise , Poluição do Ar/prevenção & controle
7.
J Environ Manage ; 364: 121323, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38889645

RESUMO

Herein, the novel double Z-scheme Ag-Ag3O4/CuO-CuFe2O4 magnetic nanophotocatalyst with nanosphere-on-nanosheet-like morphology was synthesized via the corona-plasma-assisted starch-templated microwave-combustion-precipitation method to remove the dye pollutants. The CuO-CuFe2O4 meso/macroporous nanophotocatalyst was synthesized using a one-pot-stage combustion-microwave process with/without starch as a hard-template. Subsequently, surface modification was carried out by DC corona-plasma discharge technology at various voltages, namely 500, 1000 and 1500 V. Then, the Ag3O4 photocatalyst was deposited on the CuO-CuFe2O4 fabricated with starch-hard-template and treated with 1000 V corona-plasma (denoted as: Ag-Ag3O4/CuO-CuFe2O4 (Starch) 1000 P). The properties of the synthesized nanophotocatalysts were analyzed using various techniques, including X-ray diffraction (XRD), Diffuse reflectance spectroscopy (DRS), Transmission electron microscopy (TEM), Field emission scanning electron microscopy (FESEM), Brunauer-Emmett-Teller and Barrett-Joyner-Halenda (BET-BJH), Vibrating Sample Manetometer (VSM), and Photoluminescence (PL). The XRD analysis corroborated the presence of CuO, CuFe2O4 and Ag3O4 in the structure of all samples. The BET-BJH analysis indicates that the specific surface area of the Ag-Ag3O4/CuO-CuFe2O4 (Starch) 1000 P nanophotocatalyst as the best sample is 2 m2/g, higher than other samples. Additionally, the DRS analysis revealed that the band gap of the Ag-Ag3O4/CuO-CuFe2O4 (Starch) 1000 P nanophotocatalyst is about 1.68 eV with the surface plasmon resonance. The performance of the ternary heterostructured Ag-Ag3O4/CuO-CuFe2O4 (Starch) 1000 P nanophotocatalyst was 96.2% and 89.1% in the degradation of the crystal violet (10 mg/L) and acid orange 7 (10 mg/L), respectively, proving its outstanding degradation capacity.


Assuntos
Corantes , Micro-Ondas , Amido , Corantes/química , Catálise , Amido/química , Prata/química , Cobre/química , Difração de Raios X , Gases em Plasma/química
8.
Ecotoxicol Environ Saf ; 280: 116547, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38843744

RESUMO

Deoxynivalenol (DON) is one of the most common mycotoxins distributed in food and feed, which causes severe liver injury in humans and animals. Cold atmospheric plasma (CAP) has received much attention in mycotoxin degradation due to the advantages of easy operation, high efficiency, and low temperature. So far, the majority of studies have focused on the degradation efficiency and mechanism of CAP on DON, while there is still little information available on the hepatotoxicity of DON after CAP treatment. Herein, this study aimed to investigate the effect of CAP on DON-induced hepatotoxicity both in vitro and in vivo and its underlying mechanisms. The results showed that 120-s CAP treatment achieved 97 % degradation of DON. The vitro hepatotoxicity of DON in L02 cells was significantly reduced with CAP treatment time. Meanwhile, CAP markedly alleviated DON-induced liver injury in mice including the balloon-like degeneration of liver tissues and elevation of AST and ALP level. The underlying mechanism for CAP detoxification of DON-induced hepatotoxicity was further elucidated. The results showed that DON caused severe oxidative stress in cells by suppressing the antioxidant signaling pathway of Nrf2/HO-1/NQO-1, consequently leading to mitochondrial dysfunction and cell apoptosis, accompanied by cellular senescence and inflammation. CAP blocked DON inhibition on the Nrf2/HO-1/NQO-1 signaling pathway through the efficient degradation of DON, accordingly alleviating the oxidative stress and liver injury induced by DON. Therefore, CAP is an effective method to eliminate DON hepatotoxicity, which can be applied in the detoxification of mycotoxin-contaminated food and feed to ensure human and animal health.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Gases em Plasma , Tricotecenos , Animais , Camundongos , Tricotecenos/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/patologia , Estresse Oxidativo/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/patologia , Fígado/metabolismo , Apoptose/efeitos dos fármacos , Masculino , Humanos , Inativação Metabólica , Linhagem Celular
9.
PLoS One ; 19(6): e0302578, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38829861

RESUMO

Particle-in-cell (PIC) simulation serves as a widely employed method for investigating plasma, a prevalent state of matter in the universe. This simulation approach is instrumental in exploring characteristics such as particle acceleration by turbulence and fluid, as well as delving into the properties of plasma at both the kinetic scale and macroscopic processes. However, the simulation itself imposes a significant computational burden. This research proposes a novel implementation approach to address the computationally intensive phase of the electrostatic PIC simulation, specifically the Particle-to-Interpolation phase. This is achieved by utilizing a high-speed Field Programmable Gate Array (FPGA) computation platform. The suggested approach incorporates various optimization techniques and diminishes memory access latency by leveraging the flexibility and performance attributes of the Intel FPGA device. The results obtained from our study highlight the effectiveness of the proposed design, showcasing the capability to execute hundreds of functional operations in each clock cycle. This stands in contrast to the limited operations performed in a general-purpose single-core computation platform (CPU). The suggested hardware approach is also scalable and can be deployed on more advanced FPGAs with higher capabilities, resulting in a significant improvement in performance.


Assuntos
Simulação por Computador , Eletricidade Estática , Gases em Plasma
10.
Int J Mol Sci ; 25(11)2024 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-38892174

RESUMO

Foodborne diseases can be attributed not only to contamination with bacterial or fungal pathogens but also their associated toxins. Thus, to maintain food safety, innovative decontamination techniques for toxins are required. We previously demonstrated that an atmospheric-pressure dielectric-barrier discharge (APDBD) plasma generated by a roller conveyer plasma device is effective at inactivating bacteria and fungi in foods. Here, we have further examined whether the roller conveyer plasma device can be used to degrade toxins produced by foodborne bacterial pathogens, including aflatoxin, Shiga toxins (Stx1 and Stx2), enterotoxin B and cereulide. Each toxin was spotted onto an aluminum plate, allowed to dry, and then treated with APDBD plasma applied by the roller conveyer plasma device for different time periods. Assessments were conducted using a competitive enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results demonstrate a significant time-dependent decrease in the levels of these toxins. ELISA showed that aflatoxin B1 concentrations were reduced from 308.6 µg/mL to 74.4 µg/mL within 1 min. For Shiga toxins, Stx1 decreased from 913.8 µg/mL to 65.1 µg/mL, and Stx2 from 2309.0 µg/mL to 187.6 µg/mL within the same time frame (1 min). Enterotoxin B levels dropped from 62.67 µg/mL to 1.74 µg/mL at 15 min, and 1.43 µg/mL at 30 min, but did not display a significant decrease within 5 min. LC-MS/MS analysis verified that cereulide was reduced to below the detection limit following 30 min of APDBD plasma treatment. Taken together, these findings highlight that a range of foodborne toxins can be degraded by a relatively short exposure to plasma generated by an APDBD using a roller conveyer device. This technology offers promising advancements in food safety, providing a novel method to alleviate toxin contamination in the food processing industry.


Assuntos
Pressão Atmosférica , Espectrometria de Massas em Tandem , Enterotoxinas , Depsipeptídeos/química , Microbiologia de Alimentos/métodos , Cromatografia Líquida/métodos , Doenças Transmitidas por Alimentos/prevenção & controle , Doenças Transmitidas por Alimentos/microbiologia , Ensaio de Imunoadsorção Enzimática , Contaminação de Alimentos/análise , Gases em Plasma/química , Aflatoxina B1
11.
Int J Mol Sci ; 25(11)2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38892350

RESUMO

Periodontitis is an inflammatory disease caused by Porphyromonas gingivalis (P. gingivalis) in the oral cavity. This periodontal disease causes damage to the periodontal ligament and alveolar bone and can cause tooth loss, but there is no definite treatment yet. In this study, we investigated the possibility of using no-ozone cold plasma to safely treat periodontitis in the oral cavity. First, human gingival fibroblasts (HGFs) were treated with P. gingivalis-derived lipopolysaccharide (PG-LPS) to induce an inflammatory response, and then the anti-inflammatory effect of NCP was examined, and a study was conducted to identify the mechanism of action. Additionally, the anti-inflammatory effect of NCP was verified in rats that developed an inflammatory response similar to periodontitis. When NCP was applied to PG-LPS-treated HGFs, the activities of inflammatory proteins and cytokines were effectively inhibited. It was confirmed that the process of denaturing the medium by charged particles of NCP is essential for the anti-inflammatory effect of NCP. Also, it was confirmed that repeated treatment of periodontitis rats with NCP effectively reduced the inflammatory cells and osteoclast activity. As a result, this study suggests that NCP can be directly helpful in the treatment of periodontitis in the future.


Assuntos
Anti-Inflamatórios , Fibroblastos , Gengiva , Lipopolissacarídeos , Periodontite , Porphyromonas gingivalis , Animais , Periodontite/microbiologia , Periodontite/tratamento farmacológico , Ratos , Anti-Inflamatórios/farmacologia , Humanos , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Ozônio/farmacologia , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Masculino , Citocinas/metabolismo , Modelos Animais de Doenças , Óxido Nítrico/metabolismo , Células Cultivadas
12.
Chemosphere ; 361: 142570, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38852636

RESUMO

Various contaminants of emerging concern (CECs) including pharmaceuticals and personal care products (PPCPs) have been known to threaten the aquatic ecosystem and human health even at low levels in surface water. Among them, the wide variety use of parabens as preservatives may pose potential threat to human because parabens may present estrogenic activity. Various advanced oxidation processes have been attempted to reduce parabens, but challenges using cold plasma (CP) are very rare. CP is worth paying attention to in reducing parabens because it has the advantage of generating radical ions, including reactive oxygen/nitrogen species and various ions. Accordingly, this study demonstrates how CP can be utilized and how CP competes with other advanced oxidation processes in energy requirements. Quantified ethyl-, propyl-, and butyl-paraben indicate that CP can effectively degrade them up to 99.1% within 3 h. Regression reveals that the kinetic coefficients of degradation can be increased to as high as 0.0328 min-1, comparable to other advanced oxidation processes. Many by-products generated from the oxidation of parabens provide evidence of the potential degradation pathway through CP treatment. In addition, we found that the electrical energy consumption per order of CP (39-95 kWh/m3/order) is superior to other advanced oxidation processes (69∼31,716 kWh/m3/order). Overall, these results suggest that CP may be a viable option to prevent adverse health-related consequences associated with parabens in receiving water.


Assuntos
Oxirredução , Parabenos , Poluentes Químicos da Água , Parabenos/química , Poluentes Químicos da Água/química , Gases em Plasma/química , Cinética , Conservantes Farmacêuticos/química
13.
J Appl Microbiol ; 135(6)2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38830792

RESUMO

AIMS: Cytidine, as an important commercial precursor in the chemical synthesis of antiviral and antitumor drugs, is in great demand in the market. Therefore, the purpose of this study is to build a microbial cell factory with high cytidine production. METHODS AND RESULTS: A mutant E. coli NXBG-11-F34 with high tolerance to uridine monophosphate structural analogs and good genetic stability was obtained by atmospheric room temperature plasma (ARTP) mutagenesis combined with high-throughput screening. Then, the udk and rihA genes involved in cytidine catabolism were knocked out by CRISPR/Cas9 gene editing technology, and the recombinant strain E. coli NXBG-13 was constructed. The titer, yield, and productivity of cytidine fermented in a 5 l bioreactor were 15.7 g l-1, 0.164 g g-1, and 0.327 g l-1 h-1, respectively. Transcriptome analysis of the original strain and the recombinant strain E. coli NXBG-13 showed that the gene expression profiles of the two strains changed significantly, and the cytidine de novo pathway gene of the recombinant strain was up-regulated significantly. CONCLUSIONS: ARTP mutagenesis combined with metabolic engineering is an effective method to construct cytidine-producing strains.


Assuntos
Citidina , Escherichia coli , Engenharia Metabólica , Mutagênese , Escherichia coli/genética , Escherichia coli/metabolismo , Citidina/genética , Citidina/metabolismo , Gases em Plasma , Reatores Biológicos , Edição de Genes/métodos , Sistemas CRISPR-Cas , Fermentação , Temperatura
14.
ACS Appl Mater Interfaces ; 16(27): 34480-34495, 2024 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-38923892

RESUMO

Clinical therapies, including dermatology and oncology, require safe application. In vitro experiments allow only limited conclusions about in vivo effects, while animal studies in, e.g., rodents have ethical constraints at a large scale. Chicken embryos lack pain reception until day 15 postfertilization, making the in ovo model a suitable alternative to in vivo safety assessment. In addition, the hen's egg test on chorioallantoic membrane assay allows irritation potential analysis for topical treatments, but standardized analysis has been limited so far. Medical gas plasma is a topical, routine, approved dermatology treatment. Recent work suggests the potential of this technology in oncology. Its main mode of action is the release of various reactive species simultaneously. Intriguingly, varying plasma feed gas compositions generates customized reactive species profiles previously shown to be optimized for specific applications, such as skin cancer treatment. To support clinical implications, we developed a novel chicken embryo CAM scoring and study scheme and employed the model to analyze 16 different plasma feed gas settings generated by the atmospheric pressure plasmajet kINPen, along with common anticancer drugs (e.g., cisplatin) and physiological mediators (e.g., VEGF). Extensive gas- and liquid-phase plasma reactive species profiling was done and was found to have a surprisingly low correlation with irritation potential parameters. Despite markedly different reactive species patterns, feed gas-modulated kINPen plasma was equally tolerated compared to standard argon plasma. CAM irritation with gas plasmas but not anticancer agents was reversed 48 h after treatment, underlining the only temporary tissue effects of medical gas plasma. Our results indicate a safe therapeutic application of reactive species.


Assuntos
Antineoplásicos , Membrana Corioalantoide , Gases em Plasma , Animais , Gases em Plasma/química , Embrião de Galinha , Membrana Corioalantoide/efeitos dos fármacos , Membrana Corioalantoide/metabolismo , Antineoplásicos/farmacologia , Antineoplásicos/química , Humanos , Medição de Risco , Espécies Reativas de Oxigênio/metabolismo , Galinhas
15.
Compr Rev Food Sci Food Saf ; 23(4): e13376, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38923698

RESUMO

Cold plasma treatment is an innovative technology in the food processing and preservation sectors. It is primarily employed to deactivate microorganisms and enzymes without heat and chemical additives; hence, it is often termed a "clean and green" technology. However, food quality and safety challenges may arise during cold plasma processing due to potential chemical interactions between the plasma reactive species and food components. This review aims to consolidate and discuss data on the impact of cold plasma on the chemical constituents and physical and functional properties of major food products, including dairy, meat, nuts, fruits, vegetables, and grains. We emphasize how cold plasma induces chemical modification of key food components, such as water, proteins, lipids, carbohydrates, vitamins, polyphenols, and volatile organic compounds. Additionally, we discuss changes in color, pH, and organoleptic properties induced by cold plasma treatment and their correlation with chemical modification. Current studies demonstrate that reactive oxygen and nitrogen species in cold plasma oxidize proteins, lipids, and bioactive compounds upon direct contact with the food matrix. Reductions in nutrients and bioactive compounds, including polyunsaturated fatty acids, sugars, polyphenols, and vitamins, have been observed in dairy products, vegetables, fruits, and beverages following cold plasma treatment. Furthermore, structural alterations and the generation of volatile and non-volatile oxidation products were observed, impacting the color, flavor, and texture of food products. However, the effects on dry foods, such as seeds and nuts, are comparatively less pronounced. Overall, this review highlights the drawbacks, challenges, and opportunities associated with cold plasma treatment in food processing.


Assuntos
Manipulação de Alimentos , Gases em Plasma , Gases em Plasma/química , Manipulação de Alimentos/métodos , Frutas/química , Verduras/química , Conservação de Alimentos/métodos
16.
Waste Manag ; 186: 198-204, 2024 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-38909443

RESUMO

Plasma gasification melting (PGM) provides reliable disposal of toxic medical waste with a low heating value, which is capable of converting waste into energy. This study investigates the performance of experiments on plasma gasification for the treatment of chemical-pharmaceutical medical waste (CPMW) with an air medium. A comparative analysis is performed for gasification characteristics at three reactor temperatures (1000, 1400, and 1800 °C). Moreover, a thermodynamic equilibrium model is developed to assess performance features such as syngas yield, high heating value, and cold gas efficiency in the gasification temperature range of 1000-1800 °C. A comparison of the experiment and computational outcomes shows a good agreement. The results show that the quality of syngas and heating value is improved by increasing the temperature of the plasma gasifier so that at 1800 °C, H2, CO, and higher heating value (HHV) are obtained as 41 %, 37 %, and 10 MJ/Nm3, respectively. The obtained syngas is a clean fuel with low sulfur-containing and nitrogen-containing. The experimental results provide an extensive comprehension of CPMW gasification in a plasma reactor and consider a possibility for hydrogen and energy production.


Assuntos
Resíduos de Serviços de Saúde , Resíduos de Serviços de Saúde/análise , Gases em Plasma , Eliminação de Resíduos de Serviços de Saúde/métodos , Modelos Teóricos , Gases , Termodinâmica , Hidrogênio/química
17.
Molecules ; 29(12)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38930977

RESUMO

Specialized chemicals are used for intensifying food production, including boosting meat and crop yields. Among the applied formulations, antibiotics and pesticides pose a severe threat to the natural balance of the ecosystem, as they either contribute to the development of multidrug resistance among pathogens or exhibit ecotoxic and mutagenic actions of a persistent character. Recently, cold atmospheric pressure plasmas (CAPPs) have emerged as promising technologies for degradation of these organic pollutants. CAPP-based technologies show eco-friendliness and potency for the removal of organic pollutants of diverse chemical formulas and different modes of action. For this reason, various types of CAPP-based systems are presented in this review and assessed in terms of their constructions, types of discharges, operating parameters, and efficiencies in the degradation of antibiotics and persistent organic pollutants. Additionally, the key role of reactive oxygen and nitrogen species (RONS) is highlighted. Moreover, optimization of the CAPP operating parameters seems crucial to effectively remove contaminants. Finally, the CAPP-related paths and technologies are further considered in terms of biological and environmental effects associated with the treatments, including changes in antibacterial properties and toxicity of the exposed solutions, as well as the potential of the CAPP-based strategies for limiting the spread of multidrug resistance.


Assuntos
Pressão Atmosférica , Gases em Plasma , Gases em Plasma/química , Poluentes Ambientais/química , Indústria Alimentícia , Antibacterianos/química , Antibacterianos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Compostos Orgânicos/química
18.
Int J Mol Sci ; 25(12)2024 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-38928343

RESUMO

Increasing the number of resistant bacteria resistant to treatment is one of the leading causes of death worldwide. These bacteria are created in wounds and injuries and can be transferred through hospital equipment. Various attempts have been made to treat these bacteria in recent years, such as using different drugs and new sterilization methods. However, some bacteria resist drugs, and other traditional methods cannot destroy them. In the meantime, various studies have shown that cold atmospheric plasma can kill these bacteria through different mechanisms, making cold plasma a promising tool to deactivate bacteria. This new technology can be effectively used in the food industry because it has the potential to inactivate microorganisms such as spores and microbial toxins and increase the wettability and printability of polymers to pack fresh and dried food. It can also increase the shelf life of food without leaving any residue or chemical effluent. This paper investigates cold plasma's potential, advantages, and disadvantages in the food industry and sterilization.


Assuntos
Embalagem de Alimentos , Gases em Plasma , Embalagem de Alimentos/métodos , Gases em Plasma/farmacologia , Esterilização/métodos , Pressão Atmosférica , Conservação de Alimentos/métodos , Bactérias/efeitos dos fármacos
19.
In Vivo ; 38(4): 1571-1578, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38936915

RESUMO

BACKGROUND/AIM: Cold physical plasma (CPP) has emerged as an effective therapy in oncology by inducing cytotoxic effects in various cancer cells, including chondrosarcoma (CS), Ewing's sarcoma (ES), and osteosarcoma (OS). The current study investigated the impact of CPP on cell motility in CS (CAL-78), ES (A673), and OS (U2-OS) cell lines, focusing on the actin cytoskeleton. MATERIALS AND METHODS: The CASY Cell Counter and Analyzer was used to study cell proliferation and determine the optimal concentrations of fetal calf serum to maintain viability without stimulation of cell proliferation. CellTiter-BlueCell viability assay was used to determine the effects of CPP on the viability of bone sarcoma cells. The Radius assay was used to determine cell migration. Staining for Deoxyribonuclease I, G-actin, and F-actin was used to assay for the effects on the cytoskeleton. RESULTS: Reductions in cell viability and motility were observed across all cell lines following CPP treatment. CPP induced changes in the actin cytoskeleton, leading to decreased cell motility. CONCLUSION: CPP effectively reduces the motility of bone sarcoma cells by altering the actin cytoskeleton. These findings underscore CPP's potential as a therapeutic tool for bone sarcomas and highlight the need for further research in this area.


Assuntos
Citoesqueleto de Actina , Neoplasias Ósseas , Movimento Celular , Proliferação de Células , Sobrevivência Celular , Citoesqueleto , Gases em Plasma , Humanos , Movimento Celular/efeitos dos fármacos , Gases em Plasma/farmacologia , Linhagem Celular Tumoral , Neoplasias Ósseas/patologia , Neoplasias Ósseas/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Citoesqueleto/metabolismo , Citoesqueleto de Actina/metabolismo , Citoesqueleto de Actina/efeitos dos fármacos , Osteossarcoma/patologia , Osteossarcoma/metabolismo , Actinas/metabolismo , Sarcoma/patologia , Sarcoma/metabolismo
20.
Clin Oral Investig ; 28(6): 355, 2024 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-38833072

RESUMO

OBJECTIVES: Clinical trials testing new devices require prior training on dummies to minimize the "learning curve" for patients. Dentists were trained using a novel water jet device for mechanical cleaning of dental implants and with a novel cold plasma device for surface functionalisation during a simulated open flap peri-implantitis therapy. The hypothesis was that there would be a learning curve for both devices. MATERIALS AND METHODS: 11 dentists instrumented 44 implants in a dummy-fixed jaw model. The effect of the water jet treatment was assessed as stain removal and the effect of cold plasma treatment as surface wettability. Both results were analysed using photographs. To improve treatment skills, each dentist treated four implants and checked the results immediately after the treatment as feedback. RESULTS: Water jet treatment significantly improved from the first to the second implant from 62.7% to 75.3% stain removal, with no further improvement up to the fourth implant. The wettability with cold plasma application reached immediately a high level at the first implant and was unchanged to the 4th implant (mean scores 2.7 out of 3). CONCLUSION: A moderate learning curve was found for handling of the water jet but none for handling of the cold plasma. CLINICAL RELEVANCE: Scientific rational for study: Two new devices were developed for peri-implantitis treatment (Dental water jet, cold plasma). Dentists were trained in the use of these devices prior to the trial to minimize learning effects. PRINCIPAL FINDINGS: Experienced dentists learn the handling of the water jet very rapidly and for cold plasma they do not need much training. PRACTICAL IMPLICATIONS: A clinical study is in process. When the planned clinical study will be finished, we will find out, if this dummy head exercise really minimised the learning curve for these devices.


Assuntos
Descontaminação , Implantes Dentários , Gases em Plasma , Água , Humanos , Descontaminação/métodos , Peri-Implantite/prevenção & controle , Propriedades de Superfície , Molhabilidade
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