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1.
Mar Drugs ; 20(4)2022 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-35447897

RESUMO

The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue-greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers.


Assuntos
Anelídeos , Poliquetos , Animais , Biotecnologia , Corantes/metabolismo , Humanos , Muco/química , Extratos Vegetais/análise , Poliquetos/química , Proteínas/análise
2.
Mar Pollut Bull ; 178: 113601, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35367697

RESUMO

With the increase of UV filters usage and consequent release into aquatic environments, the concerns about their potential ecological risks are also increasing. According to this, in the present study, adult polychaetes of the species Ficopomatus enigmaticus were chronically exposed to three concentrations (0.01, 0.1 and 0.5 mg/L) of organic and inorganic filters (Ethylhexyl methoxycinnamate (EHMC) and nanoparticulate Zinc oxide (nZnO), respectively) in order to analyse biochemical responses related to cellular damage, antioxidant defence, biotransformation mechanisms and, lastly, neurotoxicity. Despite major lipid peroxidation caused by EHMC was observed, both UV filters have produced the same response patterns. In details, a clear concentration-dependent activation of glutathione S-transferases and a significant decrease of acetylcholinesterase levels defined an important neurotoxic effect was observed for both contaminants. These results become important to expand the limited scientific literature on biochemical responses of marine and brackish water invertebrates to organic and inorganic UV filters.


Assuntos
Poliquetos , Poluentes Químicos da Água , Acetilcolinesterase , Animais , Peroxidação de Lipídeos , Protetores Solares/análise , Protetores Solares/toxicidade , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
3.
Nat Commun ; 13(1): 2286, 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35484126

RESUMO

Animal development is classified as conditional or autonomous based on whether cell fates are specified through inductive signals or maternal determinants, respectively. Yet how these two major developmental modes evolved remains unclear. During spiral cleavage-a stereotypic embryogenesis ancestral to 15 invertebrate groups, including molluscs and annelids-most lineages specify cell fates conditionally, while some define the primary axial fates autonomously. To identify the mechanisms driving this change, we study Owenia fusiformis, an early-branching, conditional annelid. In Owenia, ERK1/2-mediated FGF receptor signalling specifies the endomesodermal progenitor. This cell likely acts as an organiser, inducing mesodermal and posterodorsal fates in neighbouring cells and repressing anteriorising signals. The organising role of ERK1/2 in Owenia is shared with molluscs, but not with autonomous annelids. Together, these findings suggest that conditional specification of an ERK1/2+ embryonic organiser is ancestral in spiral cleavage and was repeatedly lost in annelid lineages with autonomous development.


Assuntos
Anelídeos , Poliquetos , Animais , Diferenciação Celular , Sistema de Sinalização das MAP Quinases , Mesoderma , Moluscos
4.
Mar Environ Res ; 176: 105616, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35395605

RESUMO

This work investigates whether the honeycomb worm Sabellaria alveolata (Polychaeta) actively selects foraminiferal tests to build its arenaceous bioconstructions and if the tests are chosen based on a defined criterion. To this purpose, both foraminiferal content and structure of communities were compared across samples of bioconstructions and samples of neighbouring sediment collected from two sites of southern Sicily (Central Mediterranean). Results document a higher concentration of foraminiferal tests within the tubes than in sediment, with a clear preference for biconvex and spherical morphologies. We hypothesize that the high proportion of biconvex and spherical tests in the bioconstruction is probably due to the active selection operated by the polychaete combined with the different buoyancy of foraminiferal tests kept in suspension by the wave motion. Among the grains agglutinated in the tubes, we also observed sporadic specimens of Amphistegina lobifera Larsen, a non-indigenous foraminifer native to the Red Sea.


Assuntos
Alveolados , Foraminíferos , Poliquetos , Animais , Monitoramento Ambiental , Sedimentos Geológicos/química , Oceano Índico , Sicília
5.
Zootaxa ; 5104(1): 89-110, 2022 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-35391048

RESUMO

The Brazilian Continental Margin hosts several deep seafloor landscapes. However, this deep margin is still poorly studied and monitored, compared to coastal and continental shelf areas, historically limited to areas of particular economic interest. Some studies financed by the Brazilian oil and gas company have been conducted off Southeast (SE) Brazil (Esprito Santo and Campos Basins) as part of the environmental licensing process for resource exploration. Similar results showed that annelid polychaetes dominated on the continental shelf and upper slope. Within a wide bathymetric range, Capitellidae species belonging to three different genera represented new records for Brazil: Mastobranchus Eisig, 1887, Peresiella Harmelin, 1968, and Polymastigos Green, 2002. Mastobranchus braziliensis sp. nov. was found in shallow waters (20147m) of both Esprito Santo and Campos Basins; Peresiella megapapilata sp. nov. occurred from 21 to 446m of both Esprito Santo and Campos Basins; and Polymastigos profundus sp. nov. inhabited greater depths (9901361m) of the Esprito Santo Basin only. Three new species are described and compared to other species of the respective genus, the diagnosis of some genera amended and the type material of Mastobranchus loii Gallardo, 1968 redescribed. Although the three genera have the same number of thoracic chaetigers, they present several morphological differences, and the current effort being made is to translate those differences into characters to be included on the generic diagnosis of each genus. Additionally, two new morphological characters were proposed for the family, the shape of thoracic chaetigers and depth of intersegmental groove.


Assuntos
Anelídeos , Poliquetos , Distribuição Animal , Animais , Brasil
6.
Zootaxa ; 5100(4): 482-500, 2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35391063

RESUMO

The solitary entoproct species, Loxosomella diopatricola is recorded from the Bay of Bengal, southeastern coast of India. The species was found on the eunicid polychaete Diopatra sp. collected from depth of 15 m. Loxosomella diopatricola is a medium-sized species with average body length up to 396 m, eight tentacles and oval in calyx shape. The Loxosomella diopatricola was first time recorded in India. A list of all Loxosomella species associated with polychaetes is given.


Assuntos
Baías , Poliquetos , Animais , Índia
7.
Zootaxa ; 5120(3): 334-344, 2022 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-35391164

RESUMO

Microspio moorei (Gravier, 1911), described from Admiralty Bay, King George Island (Antarctic Peninsula), has not been recorded since the original description, based on a single specimen, the holotype. Thus, subsequent comments and observations about the species have been made based only on the original description and with no information on morphological variations of the species. A re-description of this species is presented based on new material collected from a bay near the type locality, with detailed descriptions and illustrations of morphological characters. A key to all species of Microspio Mesnil, 1896 is provided. This research was part of the Colombian Antarctic Program, in collaboration with the Chilean Antarctic Institute (INACH).


Assuntos
Anelídeos , Poliquetos , Animais
8.
Zootaxa ; 5092(2): 221-237, 2022 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-35391210

RESUMO

Two new spionid polychaetes of the genus Scolelepis from the intertidal habitats of the Yellow Sea in Korea are described and illustrated. Scolelepis (Parascolelepis) anterobranchiata sp. nov. is characterized by a combination of the following characteristic: palps with smooth globular expansions at the basal part, branchiae appearing in the anterior part of the body, absence of notochaetae in chaetiger 1, notopodial postchaetal lamellae partially fused with branchiae in anterior chaetigers, and neuropodial hooded hooks appearing from chaetigers 1819 with two pairs of teeth surmounting a well-developed main fang. Scolelepis (Scolelepis) rubra sp. nov. can be distinguished from its congeners by the following characteristics: reddish-pink body, presence of notochaetae in chaetiger 1, branchiae approximately twice as long as notopodial postchaetal lamellae, neuropodial bidentate hooded hooks usually appearing from chaetigers 2631, and presence of notopodial hooded hooks in posterior chaetigers. Based on molecular data, the sequences of the two new species showed relatively large genetic distances from other Scolelepis species in COI (15.819.5%) and 16S rDNA (4.915.8%). Morphological variability of prostomial shape in the genus Scolelepis is discussed for the first time.


Assuntos
Anelídeos , Poliquetos , Animais , República da Coreia
9.
Zootaxa ; 5113(1): 1-89, 2022 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-35391387

RESUMO

Seventeen species of cirratulid polychaetes, 13 new to science, are reported from continental shelf and slope depths of the western North Atlantic Ocean. The samples were collected as part of deep-water offshore reconnaissance surveys from New England to the Carolinas and long-term monitoring programs in Boston Harbor, Massachusetts Bay, and Georges Bank off Massachusetts. The new species are included in the genera Caulleriella (C. cryptica n. sp.), Chaetocirratulus (C. hessleri n. sp., C. sandersi n. sp., and C. tomaculus n. sp.), and Chaetozone (C. adunca n. sp., C. artaspinosa n. sp., C. brychiata n. sp., C. castouria n. sp., C. donerae n. sp., C. lophia n. sp., C. novagracilis, n. sp., C. paucispinosa n. sp. and C. profunda n. sp. In addition, Chaetozone gayheadia Hartman, 1965 is redescribed based on type-material and additional collections and transferred to the genus Chaetocirratulus. Chaetozone benthaliana McIntosh, 1985 is designated a species inquirendum because it was described from a posterior fragment that cannot be confirmed as belonging to any known cirratulid genus or species. Updated descriptions and new records are provided for Chaetozone anasima, C. diodonta, and C. hystricosa all originally described by Doner Blake (2006).


Assuntos
Anelídeos , Poliquetos , Animais , Oceano Atlântico , Água
10.
Dokl Biol Sci ; 502(1): 26-30, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35298750

RESUMO

The article presents a report on the findings of representatives of frenulate pogonophorans Nereilinum murmanicum in the northern and central parts of the Barents Sea, which significantly expands the range of this species and provides guidance on its distribution in this basin. Here we present the coordinates of new finds with an indication of the depth. Find points were associated with data on known and potential hydrocarbon deposits.


Assuntos
Anelídeos , Poliquetos , Animais , Hidrocarbonetos
11.
Dokl Biol Sci ; 502(1): 42-45, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35298753

RESUMO

The anatomical and histological structure of the trophosome of the giant vestimentiferan Riftiapachyptila has been studied. The trophosome consists of longitudinally oriented cords. The cords of the trophosome intertwine, form diverticula and anastomose with each other. Each cord has an axial blood vessel inside, which is connected to afferent vessels on the surface of the cord by radial capillaries. Based on the data on the structure and development of the trophosome, it is suggested that the evolutionary precursor of the trophosome was a blood network connecting the ventral and dorsal vessels. The cells of the coelomic lining on the surface of the vessels grew and gave rise to the parenchymal tissue of the trophosome. At the same time, the trophosome developed from two sources, namely: due to the coelomic lining on the surface of the vessels of the intestinal plexus and due to the coelomic lining on the surface of the vessels of the circulatory plexus of the body wall.


Assuntos
Anelídeos , Poliquetos , Animais
12.
Int J Mol Sci ; 23(5)2022 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-35269857

RESUMO

The intertidal zone is a transitional area of the land-sea continuum, in which physical and chemical properties vary during the tidal cycle and highly toxic sulfides are rich in sediments due to the dynamic regimes. As a typical species thriving in this habitat, Urechis unicinctus presents strong sulfide tolerance and is expected to be a model species for sulfide stress research. Heat shock proteins (HSPs) consist of a large group of highly conserved molecular chaperones, which play important roles in stress responses. In this study, we systematically analyzed the composition and expression of HSPs in U. unicinctus. A total of eighty-six HSP genes from seven families were identified, in which two families, including sHSP and HSP70, showed moderate expansion, and this variation may be related to the benthic habitat of the intertidal zone. Furthermore, expression analysis revealed that almost all the HSP genes in U. unicinctus were significantly induced under sulfide stress, suggesting that they may be involved in sulfide stress response. Weighted gene co-expression network analysis (WGCNA) showed that 12 HSPs, including 5 sHSP and 4 HSP70 family genes, were highly correlated with the sulfide stress response which was distributed in steelblue and green modules. Our data indicate that HSPs, especially sHSP and HSP70 families, may play significant roles in response to sulfide stress in U. unicinctus. This systematic analysis provides valuable information for further understanding of the function of the HSP gene family for sulfide adaptation in U. unicinctus and contributes a better understanding of the species adaptation strategies of marine benthos in the intertidal zone.


Assuntos
Anelídeos , Poliquetos , Animais , Anelídeos/genética , Estudo de Associação Genômica Ampla , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Poliquetos/genética , Poliquetos/metabolismo , Sulfetos/metabolismo
13.
Mar Drugs ; 20(3)2022 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-35323465

RESUMO

Among the most potent and proteolytically resistant antimicrobial peptides (AMPs) of animal origin are molecules forming a ß-hairpin structure stabilized by disulfide bonds. In this study, we investigated the mechanism of action and therapeutic potential of the ß-hairpin AMP from the marine polychaeta Capitella teleta, named capitellacin. The peptide exhibits a low cytotoxicity toward mammalian cells and a pronounced activity against a wide range of bacterial pathogens including multi-resistant bacteria, but the mechanism of its antibacterial action is still obscure. In view of this, we obtained analogs of capitellacin and tachyplesin-inspired chimeric variants to identify amino acid residues important for biological activities. A low hydrophobicity of the ß-turn region in capitellacin determines its modest membranotropic activity and slow membrane permeabilization. Electrochemical measurements in planar lipid bilayers mimicking the E. coli membrane were consistent with the detergent-like mechanism of action rather than with binding to a specific molecular target in the cell. The peptide did not induce bacterial resistance after a 21-day selection experiment, which also pointed at a membranotropic mechanism of action. We also found that capitellacin can both prevent E. coli biofilm formation and destroy preformed mature biofilms. The marked antibacterial and antibiofilm activity of capitellacin along with its moderate adverse effects on mammalian cells make this peptide a promising scaffold for the development of drugs for the treatment of chronic E. coli infections, in particular those caused by the formation of biofilms.


Assuntos
Antibacterianos , Poliquetos/química , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , /isolamento & purificação , Organismos Aquáticos/química , Biofilmes/efeitos dos fármacos , Linhagem Celular , Permeabilidade da Membrana Celular/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Escherichia coli/fisiologia , Fibroblastos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Humanos , Conformação Proteica
14.
PLoS One ; 17(3): e0264081, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35235589

RESUMO

Meiodorvillea Jumars, 1974 is a little-known genus of Dorvilleidae Chamberlin, 1919, characterized by its small size and reduced appendages and jaw apparatus. A revision of the genus is presented, including analysis of the type material of M. minuta (Hartman, 1965) and M. apalpata Jumars, 1974, as well as specimens collected from shelf and slope continental areas in Brazil. A neotype was designated for M. minuta and its distribution was extended to Brazil. The identity of M. chilensis (Hartmann-Schröder, 1965) is questioned and three new species from 21 to 1,300.7 meters depth are also described. Meiodorvillea penhae sp. nov. has furcate chaeta only in the first anterior chaetigers. In contrast, Meiodorvillea hartmanae sp. nov. has very small palps and asymmetrical thin furcate chaeta and Meiodorvillea jumarsi sp. nov. has dorsal cirri and geniculate chaeta only in the first anterior chaetigers.


Assuntos
Anelídeos , Poliquetos , Animais , Oceano Atlântico , Brasil
15.
Artigo em Inglês | MEDLINE | ID: mdl-35278715

RESUMO

Larval attachment and metamorphosis are important processes during the development of some marine invertebrates. Myoinhibitory peptides (MIPs), a class of small molecular neuropeptides, have been revealed to be involved in regulating the larval settlement. In this paper, we identified two types of MIP membrane receptors, G-protein coupled receptor SPR and MIP-gated ion channel receptors MGIC1 and MGIC2 based on sequence homology with other species in the transcriptome database of Echiuroidea Urechis unicinctus (Xenopneusta, Urechidae). The results of in situ hybridization showed that positive signals of these receptors were obviously located in the apex of the segmentation larvae, a critical stage of U. unicinctus larval settlement. Further, these receptors were determined on the membrane of HEK293 cells by immunohistochemistry. Also, we verified that U. unicinctus MIP can activate its SPR receptor based on the results of the significantly decreased cAMP concentration in HEK293 cells. Our data will provide scientific reference for elucidating mechanism of neuropeptide regulating the larval attachment and metamorphosis in marine invertebrates.


Assuntos
Neuropeptídeos , Poliquetos , Animais , Células HEK293 , Humanos , Larva , Neuropeptídeos/genética , Neuropeptídeos/metabolismo , Poliquetos/genética , Transcriptoma
16.
Cell Death Dis ; 13(3): 214, 2022 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-35256607

RESUMO

The extremophile Alvinella pompejana, an annelid worm living on the edge of hydrothermal vents in the Pacific Ocean, is an excellent model system for studying factors that govern protein stability. Low intrinsic stability is a crucial factor for the susceptibility of the transcription factor p53 to inactivating mutations in human cancer. Understanding its molecular basis may facilitate the design of novel therapeutic strategies targeting mutant p53. By analyzing expressed sequence tag (EST) data, we discovered a p53 family gene in A. pompejana. Protein crystallography and biophysical studies showed that it has a p53/p63-like DNA-binding domain (DBD) that is more thermostable than all vertebrate p53 DBDs tested so far, but not as stable as that of human p63. We also identified features associated with its increased thermostability. In addition, the A. pompejana homolog shares DNA-binding properties with human p53 family DBDs, despite its evolutionary distance, consistent with a potential role in maintaining genome integrity. Through extensive structural and phylogenetic analyses, we could further trace key evolutionary events that shaped the structure, stability, and function of the p53 family DBD over time, leading to a potent but vulnerable tumor suppressor in humans.


Assuntos
Poliquetos , Proteína Supressora de Tumor p53 , Animais , DNA/genética , DNA/metabolismo , Filogenia , Poliquetos/química , Poliquetos/genética , Poliquetos/metabolismo , Domínios Proteicos , Proteína Supressora de Tumor p53/metabolismo
17.
J Invertebr Pathol ; 190: 107750, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35293312

RESUMO

Infestations by shell-boring polychaetes have been gaining attention in recent years due to the harmful effects that their presence can pose to the growth and production of marine bivalves worldwide. The Galician region, located in the north-western corner of the Iberian Peninsula, is one of the major producers of marine bivalves in the word and therefore highly dependent on this industry. The recent finding of an unknown polychaete boring into the shells of the king scallop, Pecten maximus, has raised the attention of producers and authorities due to its potential harm to exploitable populations and the economical losses that could entail. The results from the morphological examination revealed the spionid Polydora hoplura as the only species present. DNA of a specimen was extracted and the mitochondrial 16S rRNA gene was partially sequenced for comparison with published sequences and confirmation of the morphological identification. Consequently, we report for the first time in the area the infestation of wild harvested populations of P. maximus by the mud worm Polydora hoplura. The presence of this shell-boring cosmopolitan invader could pose a threat to king scallop exploitation in Galicia; potential economic impacts need to be evaluated.


Assuntos
Anelídeos , Bivalves , Parasitos , Pecten , Pectinidae , Poliquetos , Animais , RNA Ribossômico 16S/genética , Espanha
18.
Curr Top Dev Biol ; 147: 401-432, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35337457

RESUMO

Over the last few decades, the annelid Capitella teleta has been used increasingly as a study system for investigations of development and regeneration. Its favorable properties include an ability to continuously maintain a laboratory culture, availability of a sequenced genome, a stereotypic cleavage program of early development, substantial regeneration abilities, and established experimental and functional genomics techniques. With this review I tell of my adventure of establishing the Capitella teleta as an emerging model and share examples of a few of the contributions our work has made to the fields of evo-devo and developmental biology. I highlight examples of conservation in developmental programs as well as surprising deviations from existing paradigms that highlight the importance of leveraging biological diversity to shift thinking in the field. The story for each study system is unique, and every animal has its own advantages and disadvantages as an experimental system. Just like most progress in science, it takes strategy, hard work and determination to develop tools and resources for a less studied animal, but luck and serendipity also play a role. I include a few narratives to personalize the science, share details of the story that are not included in typical publications, and provide perspective for investigators who are interested in developing their own study organism.


Assuntos
Embrião não Mamífero , Poliquetos , Animais , Genoma , Genômica , Poliquetos/genética
19.
Curr Top Dev Biol ; 147: 497-521, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35337460

RESUMO

Investigating developmental evolution usually requires comparing differences across related species to infer how phenotypic change results from embryological modifications. However, when comparing organisms from different environments, ecologies, and evolutionary histories there can be many confounding factors to finding a genetic basis for developmental differences. In the marine annelid Streblospio benedicti, there are two distinct types of offspring with independent developmental pathways that converge on the same adult phenotype. To my knowledge, S. benedicti is the only known species that has heritable (additive) genetic variation in developmental traits that results in alternative life-history strategies. Females produce either hundreds of small, swimming and feeding larvae, or dozens of large, nonfeeding larvae. The larvae differ in their morphology, ecology, and dispersal potential. This developmental dimorphism makes S. benedicti a unique and useful model for understanding how genetic changes result in developmental modifications that ultimately lead to overall life-history differences. Because the offspring phenotypes of S. benedicti are heritable, we can use forward genetics within a single evolutionary lineage to disentangle how development evolves, and which genes and regulatory mechanisms are involved.


Assuntos
Modelos Genéticos , Poliquetos , Animais , Evolução Biológica , Feminino , Larva/genética , Fenótipo , Poliquetos/genética
20.
Zoolog Sci ; 39(1): 99-105, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35106997

RESUMO

A new species of amphinomid polychaete, Branchamphinome kohtsukai sp. nov., is described from Japanese waters, 29-211 m in depth. The species is distinguishable from other congeners by the following features: i) branchiae with four-six filaments in midbody segments; ii) two pairs of eyes not coalescent; iii) the ventral side of the first four chaetigers broadly pigmented. This is the first record of Branchamphinome from the North Pacific Ocean. We provide a phylogenetic tree based on the sequences of four genes (COI, 16S, 18S, 28S) and discuss amphinomids' relationships.


Assuntos
Anelídeos , Poliquetos , Animais , Anelídeos/genética , Oceano Pacífico , Filogenia , Poliquetos/genética
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