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1.
Environ Sci Pollut Res Int ; 31(32): 45192-45203, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38961018

RESUMO

Exposure to organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) has been reported to be associated with renal impairment and chronic kidney disease (CKD). Nevertheless, the research results thus far have exhibited inconsistency, and the effect of lifestyle on their association is not clear. In this study, we assessed the correlation between serum OCPs/PCBs and CKD and renal function indicators including estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) among 1721 Chinese adults. In order to further investigate the potential impact of lifestyle, we conducted joint associations of lifestyle and OCPs/PCBs on CKD. We found a negative correlation between p,p'-DDE and eGFR, while logistic regression results showed a positive correlation between PCB-153 and CKD (OR, 1.92; 95% CI, 1.21, 3.06). Quantile g-computation regression analyses showed that the association between co-exposure to OCPs/PCBs and CKD was not significant, but p,p'-DDE and PCB-153 were the main contributors to the negative and positive co-exposure effects of eGFR and CKD, respectively, which is consistent with the regression results. Participants with both relatively high PCB-153 exposure and an unhealthy lifestyle had the highest risk of CKD, in the joint association analysis. The observed associations were generally supported by the FAS-eGFR method. Our research findings suggest that exposure to OCPs/PCBs may be associated with decreased eGFR and increased prevalence of CKD in humans, and a healthy lifestyle can to some extent alleviate the adverse association between PCB-153 exposure and CKD.


Assuntos
Hidrocarbonetos Clorados , Estilo de Vida , Praguicidas , Bifenilos Policlorados , Insuficiência Renal Crônica , Humanos , Insuficiência Renal Crônica/induzido quimicamente , Insuficiência Renal Crônica/epidemiologia , Bifenilos Policlorados/sangue , Adulto , Masculino , Feminino , Pessoa de Meia-Idade , Exposição Ambiental , Taxa de Filtração Glomerular , China , Idoso , Poluentes Ambientais
2.
J Agric Food Chem ; 72(28): 15643-15652, 2024 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-38967173

RESUMO

Gas chromatography with electron capture negative ion mass spectrometry (GC/ECNI-MS) was used to quantify and compare halogenated natural products (HNPs) and selected anthropogenic persistent organic pollutants (POPs) in individual samples of 17 fish species from the Seychelles (Western Indian Ocean). The sum-HNP amounts (9.5-1100 ng/g lipid mass (lm)) were between 1 and 2 orders of magnitude higher than those of the sum of seven abundant polychlorinated biphenyl (PCB) congeners (0.2-15 ng/g lm) and dichlorodiphenyltrichloroethane-related compounds (DDTs) (<1.1-43 ng/g lm). Within the group of HNPs, the two tetrabrominated phenoxyanisoles (aka methoxylated diphenyl ethers, MeO-BDEs), 2'-MeO-BDE 68 ≫ 6-MeO-BDE 47, were predominant in most cases. Pearson correlation analysis showed that MeO-BDE levels were positively correlated with less abundant HNPs (2,2'-diMeO-BB 80, 2',6-diMeO-BDE 68, and Br6-DBP) (p < 0.01). Accordingly, HNPs, rather than PCBs and DDTs, were the predominant polyhalogenated contaminants in the current species.


Assuntos
Produtos Biológicos , Peixes , Bifenilos Policlorados , Poluentes Químicos da Água , Animais , Peixes/metabolismo , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Produtos Biológicos/metabolismo , Produtos Biológicos/química , Bifenilos Policlorados/metabolismo , Bifenilos Policlorados/análise , Bifenilos Policlorados/química , Oceano Índico , Seicheles , Bioacumulação , Cromatografia Gasosa-Espectrometria de Massas , Recifes de Corais
3.
Environ Int ; 189: 108777, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38838491

RESUMO

INTRODUCTION: Arctic Monitoring and Assessment Programme (AMAP) monitors persistent organic pollutant (POP) levels in the Arctic populations and assesses health effects related to exposure to them. Many internationally regulated POPs persist in humans and biota, while new Emerging Contaminants of Arctic Concern (ECAC), many of which are unregulated, present additional challenges. Biomonitoring offers valuable insights into temporal trends within human matrices, revealing critical information not only about the efficacy of international regulations but also serving as an early warning system for exposure and risks for human health. METHODS: Data analyzed in this study is aggregated data presented in the AMAP Human Health in the Arctic assessments, which provide data on contaminant concentrations measured in human matrices from adults, and children across various population studies conducted in the Arctic since the 1980 s. Linear regression analyses were used to assess trends of various POPs including organochlorine (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and per- and polyfluoroalkyl substances (PFAS), measured over time from the Arctic populations in Finland, Norway, Sweden, Denmark, Iceland, Canada and Alaska (USA). RESULTS: Overall, decreasing trends were observed for PCBs and OCPs. Regulated PFAS showed decreasing trends, but increasing trends were observed for unregulated PFAS in certain populations. PBDEs showed decreasing or inconsistent trends in certain Arctic populations. CONCLUSIONS: Decreasing trends are observed for legacy POPs, but the trends for new emerging contaminants are inconsistent. More focus is needed on biomonitoring the new emerging contaminants of concern in the Arctic and their implications on human health.


Assuntos
Monitoramento Ambiental , Humanos , Regiões Árticas , Poluentes Ambientais/análise , Adulto , Criança , Éteres Difenil Halogenados/análise , Exposição Ambiental , Bifenilos Policlorados/análise , Hidrocarbonetos Clorados/análise , Poluentes Orgânicos Persistentes , Monitoramento Biológico
4.
Biosensors (Basel) ; 14(6)2024 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-38920592

RESUMO

The scope of this study was to apply advances in materials science, specifically the use of organosilicate nanoparticles as a high surface area platform for passive sampling of chemicals or pre-concentration for active sensing in multiple-phase complex environmental media. We have developed a novel nanoporous organosilicate (NPO) film as an extraction phase and proof of concept for application in adsorbing hydrophobic compounds in water and sediment. We characterized the NPO film properties and provided optimization for synthesis and coatings in order to apply the technology in environmental media. NPO films in this study had a very high surface area, up to 1325 m2/g due to the high level of mesoporosity in the film. The potential application of the NPO film as a sorbent phase for sensors or passive samplers was evaluated using a model hydrophobic chemical, polychlorinated biphenyls (PCB), in water and sediment. Sorption of PCB to this porous high surface area nanoparticle platform was highly correlated with the bioavailable fraction of PCB measured using whole sediment chemistry, porewater chemistry determined by solid-phase microextraction fiber methods, and the Lumbriculus variegatus bioaccumulation bioassay. The surface-modified NPO films in this study were found to highly sorb chemicals with a log octanol-water partition coefficient (Kow) greater than four; however, surface modification of these particles would be required for application to other chemicals.


Assuntos
Sedimentos Geológicos , Interações Hidrofóbicas e Hidrofílicas , Nanopartículas , Nanopartículas/química , Água/química , Bifenilos Policlorados/análise , Poluentes Químicos da Água/análise , Compostos de Organossilício/química , Adsorção , Propriedades de Superfície
5.
PLoS One ; 19(6): e0298504, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38913645

RESUMO

INTRODUCTION: Chemical contamination and pollution are an ongoing threat to human health and the environment. The concern over the consequences of chemical exposures at the global level continues to grow. Because resources are constrained, there is a need to prioritize interventions focused on the greatest health impact. Data, especially related to chemical exposures, are rarely available for most substances of concern, and alternate methods to evaluate their impact are needed. STRUCTURED EXPERT JUDGMENT (SEJ) PROCESS: A Structured Expert Judgment (Research Outreach, 2021) process was performed to provide plausible estimates of health impacts for 16 commonly found pollutants: asbestos, arsenic, benzene, chromium, cadmium, dioxins, fluoride, highly hazardous pesticides (HHPs), lead, mercury, polycyclic-aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), Per- and Polyfluorinated Substances (PFAs), phthalates, endocrine disrupting chemicals (EDCs), and brominated flame retardants (BRFs). This process, undertaken by sector experts, weighed individual estimations of the probable global health scale health impacts of each pollutant using objective estimates of the expert opinions' statistical accuracy and informativeness. MAIN FINDINGS: The foremost substances, in terms of mean projected annual total deaths, were lead, asbestos, arsenic, and HHPs. Lead surpasses the others by a large margin, with an estimated median value of 1.7 million deaths annually. The three other substances averaged between 136,000 and 274,000 deaths per year. Of the 12 other chemicals evaluated, none reached an estimated annual death count exceeding 100,000. These findings underscore the importance of prioritizing available resources on reducing and remediating the impacts of these key pollutants. RANGE OF HEALTH IMPACTS: Based on the evidence available, experts concluded some of the more notorious chemical pollutants, such as PCBs and dioxin, do not result in high levels of human health impact from a global scale perspective. However, the chemical toxicity of some compounds released in recent decades, such as Endocrine Disrupters and PFAs, cannot be ignored, even if current impacts are limited. Moreover, the impact of some chemicals may be disproportionately large in some geographic areas. Continued research and monitoring are essential; and a preventative approach is needed for chemicals. FUTURE DIRECTIONS: These results, and potential similar analyses of other chemicals, are provided as inputs to ongoing discussions about priority setting for global chemicals and pollution management. Furthermore, we suggest that this SEJ process be repeated periodically as new information becomes available.


Assuntos
Poluentes Ambientais , Humanos , Poluentes Ambientais/toxicidade , Poluentes Ambientais/análise , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Prova Pericial , Disruptores Endócrinos/toxicidade , Praguicidas/toxicidade , Bifenilos Policlorados/análise , Bifenilos Policlorados/toxicidade , Arsênio/análise , Arsênio/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Poluição Ambiental/análise , Amianto/efeitos adversos , Dioxinas/toxicidade , Dioxinas/análise
6.
Anal Chim Acta ; 1315: 342756, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38879204

RESUMO

BACKGROUND: Dried blood spots (DBSs) collected and archived in newborn screening programs (NSP) represent a potentially valuable resource for assessing exposure to a range of organic and inorganic chemicals in newborns. This study develops and optimizes a method to measure polychlorinated naphthalenes (PCNs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and organochlorine pesticides (OCPs) in DBS using the isotope dilution technique, ultrasonic-assisted liquid-liquid extraction, simple cleanup, triple quadrupole GC-MS/MS analysis, and background correction. RESULTS: We minimize the number of extraction repetitions and the volume of solvent, which helps increase throughput while minimizing the potential for contamination. We obtained high recovery and precision for most compounds, and method detection limits (MDLs) were sufficiently low to detect the more prevalent compounds based on representative sample of the US population. MDLs averaged 0.020 ng/mL (recovery: 107 %, precision: 4 %) for PCNs, 0.021 ng/mL (recovery: 97 %, precision: 4 %) for PCBs, 0.021 ng/mL (recovery: 117 %, precision: 2 %) for OCPs, and 0.021 ng/mL (recovery: 96 %, precision: 3 %) for PBDEs. SIGNIFICANCE AND NOVELTY: To our knowledge, this is the first study presenting an analytical method and for PCNs in DBS, and one of the few studies providing an assessment of method performance for persistent organic pollutants in DBS. The optimized method can be applied to a wide range of applications, including exposure assessment, environmental epidemiology, forensics, environmental surveillance, and ecological monitoring.


Assuntos
Teste em Amostras de Sangue Seco , Naftalenos , Poluentes Orgânicos Persistentes , Espectrometria de Massas em Tandem , Teste em Amostras de Sangue Seco/métodos , Humanos , Naftalenos/sangue , Poluentes Orgânicos Persistentes/sangue , Espectrometria de Massas em Tandem/métodos , Éteres Difenil Halogenados/sangue , Éteres Difenil Halogenados/análise , Bifenilos Policlorados/sangue , Bifenilos Policlorados/análise , Extração Líquido-Líquido/métodos , Hidrocarbonetos Clorados/sangue , Hidrocarbonetos Clorados/análise , Recém-Nascido , Cromatografia Gasosa-Espectrometria de Massas/métodos , Limite de Detecção , Praguicidas/sangue , Praguicidas/análise
7.
Bioresour Technol ; 404: 130918, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38823562

RESUMO

Symbiosis between Glycine max and Bradyrhizobium diazoefficiens were used as a model system to investigate whether biohydrogen utilization promotes the transformation of the tetrachlorobiphenyl PCB77. Both a H2 uptake-positive (Hup+) strain (wild type) and a Hup- strain (a hupL deletion mutant) were inoculated into soybean nodules. Compared with Hup- nodules, Hup+ nodules increased dechlorination significantly by 61.1 % and reduced the accumulation of PCB77 in nodules by 37.7 % (p < 0.05). After exposure to nickel, an enhancer of uptake hydrogenase, dechlorination increased significantly by 2.2-fold, and the accumulation of PCB77 in nodules decreased by 54.4 % (p < 0.05). Furthermore, the tetrachlorobiphenyl transformation in the soybean root nodules was mainly testified to be mediated by nitrate reductase (encoded by the gene NR) for tetrachlorobiphenyl dechlorination and biphenyl-2,3-diol 1,2-dioxygenase (bphC) for biphenyl degradation. This study demonstrates for the first time that biohydrogen utilization has a beneficial effect on tetrachlorobiphenyl biotransformation in a legume-rhizobium symbiosis.


Assuntos
Glycine max , Hidrogênio , Bifenilos Policlorados , Simbiose , Bifenilos Policlorados/metabolismo , Simbiose/fisiologia , Glycine max/metabolismo , Glycine max/microbiologia , Hidrogênio/metabolismo , Rhizobium/fisiologia , Biotransformação , Bradyrhizobium/metabolismo , Bradyrhizobium/fisiologia , Biodegradação Ambiental
8.
J Hazard Mater ; 475: 134862, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-38885585

RESUMO

The composition and metabolites of the gut microbiota can be altered by environmental pollutants. However, the effect of co-exposure to multiple pollutants on the human gut microbiota has not been sufficiently studied. In this study, gut microorganisms and their metabolites were compared between 33 children from Guiyu, an e-waste dismantling and recycling area, and 34 children from Haojiang, a healthy environment. The exposure level was assessed by estimating the daily intake (EDI) of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), 6PPD-quinone (6PPDQ), and metal(loid)s in kindergarten dust. Significant correlations were found between the EDIs of 6PPDQ, BDE28, PCB52, Ni, Cu, and the composition of gut microbiota and specific metabolites. The Bayesian kernel machine regression model showed negative correlations between the EDIs of five pollutants (6PPDQ, BDE28, PCB52, Ni, and Cu) and the composition of gut microbiota. The EDIs of these five pollutants were positively correlated with the levels of the metabolite 2,4-diaminobutyric acid, while negatively correlated with the levels of d-erythro-sphingosine and d-threitol. Our study suggests that exposure to 6PPDQ, BDE28, PCB52, Ni, and Cu in kindergarten dust is associated with alterations in the composition and metabolites of the gut microbiota. These alterations may be associated with children's health.


Assuntos
Poluentes Ambientais , Microbioma Gastrointestinal , Éteres Difenil Halogenados , Bifenilos Policlorados , Humanos , Éteres Difenil Halogenados/toxicidade , Microbioma Gastrointestinal/efeitos dos fármacos , Bifenilos Policlorados/toxicidade , Bifenilos Policlorados/metabolismo , Feminino , Masculino , Criança , Poluentes Ambientais/toxicidade , Poluentes Ambientais/metabolismo , Poeira/análise , Pré-Escolar , Exposição Ambiental , Metabolômica , Resíduo Eletrônico , China , Metais/metabolismo , Metais/toxicidade , Organofosfatos/toxicidade , Organofosfatos/metabolismo
9.
Anal Methods ; 16(25): 4160-4167, 2024 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-38874006

RESUMO

This study proposes a strategy using a microfluidic ratiometric electrochemical aptasensor to detect PCB77 with excellent sensitivity and specificity. This sensing platform combines a microfluidic chip, a wireless integrated circuit system for aptamer-based electrochemical detection, and a mobile phone control terminal for parameter configuration, identification, observation, and wireless data transfer. The sensing method utilizes a cDNA (MB-COOH-cDNA-SH) that is labelled with the redox probe Methylene Blue (MB) at the 5' end and has a thiol group at the 3' end. Additionally, it utilizes a single strand PCB aptamer that has been modified with ferrocenes at the 3' end (aptamer-Fc). Through gold-thiol binding, the labelled probe of MB-COOH-cDNA-SH was self-assembled onto the surface of an Au/Nb2CTx/GO modified electrode. On exposure to aptamer-Fc, it will hybridize with MB-COOH-cDNA-SH to form a stable double-stranded structure on the electrode surface. When PCB77 is present, aptamer-Fc binds specifically to the target, enabling the double-stranded DNA to unwind. Such variation caused changes in the differential pulse voltammetry (DPV) peak currents of both MB and Fc. A substantial improvement is observed in the ratio between the two DPV peaks. Under the optimum experimental conditions, this assay has a response that covers the 0.0001 to 1000 ng mL-1 PCB77 concentration range, and the detection limit is 1.56 × 10-5 ng mL-1. The integration of a ratiometric electrochemical aptasensor with designed microfluidic and integrated devices in this work is an innovative and promising approach that offers an efficient platform for on-site applications.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Técnicas Eletroquímicas , Bifenilos Policlorados , Aptâmeros de Nucleotídeos/química , Bifenilos Policlorados/análise , Bifenilos Policlorados/química , Técnicas Eletroquímicas/métodos , Técnicas Eletroquímicas/instrumentação , Técnicas Biossensoriais/métodos , Limite de Detecção , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Ouro/química , Azul de Metileno/química , Compostos Ferrosos/química , Eletrodos
10.
Environ Sci Pollut Res Int ; 31(31): 43996-44004, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38922474

RESUMO

The white stork Ciconia ciconia is a bird species located at the top of the trophic pyramid in grassland and wetland ecosystems. This charismatic species is susceptible to pesticides and their environmental residues. In 2016, we collected blood samples from 114 white stork chicks across Western and Southern Poland. Chicks were sexed by molecular analysis and aged by development pattern. We studied the relationship between the concentration of pesticides (beta-HCH, heptachlor, aldrin, endrin, 4.4'-DDD, 4.4'-DDE and 4.4'-DDT) and of PCB in the chicks' blood with blood morphology and biochemistry parameters in the blood. The mean (± SD) values of concentrations of above detection level pesticides were: for (1) beta-HCH 4.139 ± 19.205; (2) 4.4'-DDE 9.254 ± 91.491 and additionally (3) PCB 16.135 ± 44.777 ppb. We found negative relationships between beta-HCH and oxidative stress enzyme activity in the blood, between beta-HCH and leukocyte concentration and between 4.4'-DDE and catalase activity. We also found a positive relationship between the concentration of pesticides in blood and the age of chicks. Interestingly, we found a higher concentration of PCB in the blood of male stork chicks than in female stork chicks. We provide more evidence that the presence of pesticides in the environment can be a strong stress factor, shaping the health status of birds.


Assuntos
Aves , Estresse Oxidativo , Praguicidas , Bifenilos Policlorados , Animais , Polônia , Estresse Oxidativo/efeitos dos fármacos , Aves/sangue , Bifenilos Policlorados/sangue , Monitoramento Ambiental , Poluentes Ambientais/sangue
11.
Sci Total Environ ; 931: 172911, 2024 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-38705305

RESUMO

Breastfeeding provides numerous health benefits for both infants and mothers, promoting optimal growth and development while offering protection against various illnesses and diseases. This study investigated the levels of polychlorinated biphenyls (PCB), organochlorine pesticides (OCP) and polycyclic aromatic hydrocarbons (PAH) in human milk sampled in Zadar (Croatia). The primary objectives were twofold: firstly, to evaluate the individual impact of each compound on the total antioxidant capacity (TAC) value, and secondly, to assess associated health risks. Notably, this study presents pioneering and preliminary insights into PAH levels in Croatian human milk, contributing to the limited research on PAH in breast milk worldwide. PCB and OCP levels in Croatian human milk were found to be relatively lower compared to worldwide data. Conversely, PAH levels were comparatively higher, albeit with lower detection frequencies. A negative correlation was established between organic contaminant levels and antioxidative capacity, suggesting a potential link between higher antioxidative potential and lower organic contaminant levels. Diagnostic ratio pointed towards traffic emissions as the primary source of the detected PAH. The presence of PAH suggests potential health risk, underscoring the need for further in-depth investigation.


Assuntos
Antioxidantes , Hidrocarbonetos Clorados , Leite Humano , Hidrocarbonetos Policíclicos Aromáticos , Leite Humano/química , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Clorados/análise , Medição de Risco , Croácia , Feminino , Poluentes Orgânicos Persistentes , Praguicidas/análise , Monitoramento Ambiental , Bifenilos Policlorados/análise , Adulto , Poluentes Ambientais/análise
12.
Sci Total Environ ; 933: 173020, 2024 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-38719047

RESUMO

This study investigated 32 persistent organic pollutants, including 9 organochlorine pesticides, 15 polychlorinated biphenyls, 6 polycyclic aromatic hydrocarbons, and 2 organophosphate pesticides in the hair samples of domestic cats and dogs living in an urban area in Samsun, Turkiye. Hair samples were collected from 35 cats and 38 dogs, grouped by sex and age (<3 or >3 years old). Samples were extracted using a liquid-liquid extraction method and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results revealed the presence of organochlorine pesticides (n = 58, 468.65 ± 92.30 ng/g), polycyclic aromatic hydrocarbons (n = 57, 15.65 ± 3.91 ng/g), polychlorinated biphenyls (n = 55, 54.11 ± 9.47 ng/g), and organophosphate pesticides (n = 25, 568.43 ± 113.17 ng/g) in the samples. PCBs 81, 118, 128, 208, and 2,4-DDE were not detected in any samples. Only one sample did not contain any of the searched compounds. Fluorene was the most frequently detected pollutant (n = 53, 72.6 %), followed by ß-hexachlorocyclohexane (n = 34, 46.6 %). The highest maximum concentration was observed for hexachlorobenzene (2748.03 ng/g), followed by aldrin (2313.45 ng/g) and fenitrothion (2081.13 ng/g). Pollutant concentrations did not differ between cats and dogs, sexes, and ages (p > 0.05). This study highlights the significant threat that urban areas pose to pets, and therefore, POPs should be monitored periodically in hair and other samples. To the best of our knowledge, this is the first report to investigate POP levels in hair samples from cats and dogs in Turkiye.


Assuntos
Monitoramento Ambiental , Cabelo , Poluentes Orgânicos Persistentes , Bifenilos Policlorados , Animais , Cães , Gatos , Cabelo/química , Monitoramento Ambiental/métodos , Bifenilos Policlorados/análise , Masculino , Feminino , Praguicidas/análise , Turquia , Hidrocarbonetos Policíclicos Aromáticos/análise , Cromatografia Gasosa-Espectrometria de Massas , Hidrocarbonetos Clorados/análise , Poluentes Ambientais/análise
13.
J Hazard Mater ; 472: 134563, 2024 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-38735186

RESUMO

Persistent organic pollutants (POPs), such as perfluoroalkyl and polyfluoroalkyl substances (PFASs), polychlorinated biphenyls (PCBs), and bisphenols (BPs), have been raising global concerns due to their toxic effects on environment and human health. The monitoring of residues of POPs in seafood is crucial for assessing the accumulation of these contaminants in the study area and mitigating potential risks to human health. However, the diversity and complexity of POPs in seafood present significant challenges for their simultaneous detection. Here, a novel multi-component fluoro-functionalized covalent organic framework (OH-F-COF) was designed as SPE adsorbent for simultaneous extraction POPs. On this basis, the recognition and adsorption mechanisms were investigated by molecular simulation. Due to multiple interactions and large specific surface area, OH-F-COF displayed satisfactory coextraction performance for PFASs, PCBs, and BPs. Under optimized conditions, the OH-F-COF sorbent was employed in a strategy of simultaneous extraction and stepwise elution (SESE), in combination with HPLC-MS/MS and GC-MS method, to effectively determined POPs in seafood collected from coastal areas of China. The method obtained low detection limits for BPs (0.0037 -0.0089 ng/g), PFASs (0.0038 -0.0207 ng/g), and PCBs (0.2308 -0.2499 ng/g), respectively. This approach provided new research ideas for analyzing and controlling multitarget POPs in seafood. ENVIRONMENTAL IMPLICATIONS: Persistent organic pollutants (POPs), such as perfluoroalkyl and polyfluoroalkyl substances (PFASs), polychlorinated biphenyls (PCBs), and bisphenols (BPs), have caused serious hazards to human health and ecosystems. Hence, there is a need to develop a quantitative method that can rapidly detect POPs in environmental and food samples. Herein, a novel multi-component fluorine-functionalized covalent organic skeletons (OH-F-COF) were prepared at room temperature, and served as adsorbent for POPs. The SESE-SPE strategy combined with chromatographic techniques was used to achieve a rapid detection of POPs in sea foods from the coastal provinces of China. This method provides a valuable tool for analyzing POPs in environmental and food samples.


Assuntos
Contaminação de Alimentos , Alimentos Marinhos , Extração em Fase Sólida , Alimentos Marinhos/análise , Extração em Fase Sólida/métodos , Adsorção , Contaminação de Alimentos/análise , Poluentes Químicos da Água/análise , Poluentes Orgânicos Persistentes/química , Estruturas Metalorgânicas/química , Fenóis/análise , Fenóis/isolamento & purificação , Bifenilos Policlorados/análise , Espectrometria de Massas em Tandem , Cromatografia Gasosa-Espectrometria de Massas , Cromatografia Líquida de Alta Pressão , Animais
14.
Sci Total Environ ; 933: 173212, 2024 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-38759481

RESUMO

Persistent organic pollutants (POPs), such as polychlorinated diphenyls (PCBs) and brominated diphenyl ethers (PBDEs), are ubiquitous in the pet cat's living environment and are ingested through dietary intake and environmental exposure such as house dust. Cats are known to be susceptible to chronic kidney disease (CKD) and exposure to POPs may be associated with CKD. However, no studies have been conducted on the renal accumulation and health effects of POPs in cats. The objective of this study was to elucidate the accumulation of PCBs, PBDEs, and organochlorine pesticides (OCPs) in the kidneys of domestic cats and discuss their potential impact on feline health. We report here that cats specifically accumulate POPs in their kidneys. Tissue samples were collected from the kidneys, livers, and muscles of cats and the concentrations of POPs in these tissues were analyzed in this study. The results showed that these compounds accumulated significantly higher in the kidney compared to other tissues. In addition, the ability to accumulate in the kidney was higher in cats than in other animals, suggesting that cats have a unique pattern of POPs accumulation in their kidneys, which is thought to occur because cats store a significant number of lipid droplets in the proximal tubules of the kidneys. This unique feature suggests that lipophilic POPs may accumulate in these lipid droplets during the excretory process. Accumulation of certain POPs in the kidneys causes necrosis and sloughing of renal tubular epithelial cells, which may be associated with CKD, a common disease in cats. This study provides valuable insight into understanding the renal accumulation and risk of POPs in cats and provides essential knowledge for developing strategies to protect the health and welfare of domestic cats.


Assuntos
Éteres Difenil Halogenados , Rim , Poluentes Orgânicos Persistentes , Animais , Gatos , Rim/metabolismo , Éteres Difenil Halogenados/metabolismo , Poluentes Orgânicos Persistentes/metabolismo , Bifenilos Policlorados/metabolismo , Exposição Ambiental , Praguicidas/metabolismo , Animais de Estimação , Hidrocarbonetos Clorados/metabolismo , Poluentes Ambientais/metabolismo
15.
Ecotoxicol Environ Saf ; 278: 116419, 2024 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-38718726

RESUMO

3,3',4,4',5-Pentachlorobiphenyl (PCB126) is the most toxic congener of dioxin-like polychlorinated biphenyls (DL PCBs), while nanoplastics (NPs) have recently emerged as significant marine pollutants, both posing threats to aquatic organisms and human health. They coexist in the environment, but their comprehensive toxicological effects remain unclear. In this study, zebrafish embryos were simultaneously exposed to PCB126 and 80-nanometer nanoplastyrene (NPS). Researchers utilized fluorescence microscopy, qPCR, histopathological examination, and transcriptomic sequencing to investigate the developmental toxicity of different concentrations of PCB126 and NPS individually or in combination on zebrafish embryos and larvae. Results indicate that the chorion significantly impedes the accumulation of NPS (p < 0.05). It is noteworthy that this barrier effect diminishes upon simultaneous exposure to PCB126. In this experiment, the semi-lethal concentration of PCB126 for larvae was determined to be 6.33 µg/L. Exposure to PCB126 induces various deformities, primarily mediated through the aryl hydrocarbon receptor (AHR). Similarly, exposure to NPS also activates AHR, leading to developmental impairments. Furthermore, transcriptomic sequencing revealed similar effects of PCB126 and NPS on the gene expression trends in zebrafish larvae, but combined exposure to both exacerbates the risk of cancer and induces more severe cardiac toxicity. At this level, co-exposure to PCB126 and NPS adversely affects the development of zebrafish larvae. This study contributes to a deeper understanding of the in vivo accumulation of DL polychlorinated biphenyls and microplastics in actual aquatic environments and their impact on fish development.


Assuntos
Larva , Bifenilos Policlorados , Poliestirenos , Poluentes Químicos da Água , Peixe-Zebra , Animais , Bifenilos Policlorados/toxicidade , Larva/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Poliestirenos/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Microplásticos/toxicidade , Nanopartículas/toxicidade
16.
Environ Int ; 187: 108710, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38701644

RESUMO

Exposure to persistent organic pollutants (POPs), such as dichlorodiphenyltrichloroethane (DDT) and polychlorinated biphenyls (PCBs), has historically been linked to population collapses in wildlife. Despite international regulations, these legacy chemicals are still currently detected in women of reproductive age, and their levels correlate with reduced ovarian reserve, longer time-to-pregnancy, and higher risk of infertility. However, the specific modes of action underlying these associations remain unclear. Here, we examined the effects of five commonly occurring POPs - hexachlorobenzene (HCB), p,p'-dichlorodiphenyldichloroethylene (DDE), 2,3,3',4,4',5-hexachlorobiphenyl (PCB156), 2,2',3,4,4',5,5'-heptachlorobiphenyl (PCB180), perfluorooctane sulfonate (PFOS) - and their mixture on human ovaries in vitro. We exposed human ovarian cancer cell lines COV434, KGN, and PA1 as well as primary ovarian cells for 24 h, and ovarian tissue containing unilaminar follicles for 6 days. RNA-sequencing of samples exposed to concentrations covering epidemiologically relevant levels revealed significant gene expression changes related to central energy metabolism in the exposed cells, indicating glycolysis, oxidative phosphorylation, fatty acid metabolism, and reactive oxygen species as potential shared targets of POP exposures in ovarian cells. Alpha-enolase (ENO1), lactate dehydrogenase A (LDHA), cytochrome C oxidase subunit 4I1 (COX4I1), ATP synthase F1 subunit alpha (ATP5A), and glutathione peroxidase 4 (GPX4) were validated as targets through qPCR in additional cell culture experiments in KGN. In ovarian tissue cultures, we observed significant effects of exposure on follicle growth and atresia as well as protein expression. All POP exposures, except PCB180, decreased unilaminar follicle proportion and increased follicle atresia. Immunostaining confirmed altered expression of LDHA, ATP5A, and GPX4 in the exposed tissues. Moreover, POP exposures modified ATP production in KGN and tissue culture. In conclusion, our results demonstrate the disruption of cellular energy metabolism as a novel mode of action underlying POP-mediated interference of follicle growth in human ovaries.


Assuntos
Metabolismo Energético , Fluorocarbonos , Ovário , Poluentes Orgânicos Persistentes , Humanos , Feminino , Ovário/efeitos dos fármacos , Ovário/metabolismo , Metabolismo Energético/efeitos dos fármacos , Fluorocarbonos/toxicidade , Homeostase/efeitos dos fármacos , Linhagem Celular Tumoral , Bifenilos Policlorados/toxicidade , Diclorodifenil Dicloroetileno/toxicidade , Ácidos Alcanossulfônicos/toxicidade , Hexaclorobenzeno/toxicidade
17.
Mol Biol Rep ; 51(1): 624, 2024 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-38710963

RESUMO

BACKGROUND: Thyroid hormones are primarily responsible for the brain development in perinatal mammals. However, this process can be inhibited by external factors such as environmental chemicals. Perinatal mammals are viviparous, which makes direct fetal examination difficult. METHODS: We used metamorphic amphibians, which exhibit many similarities to perinatal mammals, as an experimental system. Therefore, using metamorphic amphibians, we characterized the gene expression of matrix metalloproteinases, which play an important role in brain development. RESULTS: The expression of many matrix metalloproteinases (mmps) was characteristically induced during metamorphosis. We also found that the expression of many mmps was induced by T3 and markedly inhibited by hydroxylated polychlorinated biphenyls (PCBs). CONCLUSION: Overall, our findings suggest that hydroxylated PCBs disrupt normal brain development by disturbing the gene expression of mmps.


Assuntos
Encéfalo , Metaloproteinases da Matriz , Metamorfose Biológica , Bifenilos Policlorados , Hormônios Tireóideos , Xenopus laevis , Animais , Encéfalo/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/crescimento & desenvolvimento , Xenopus laevis/metabolismo , Xenopus laevis/genética , Metaloproteinases da Matriz/metabolismo , Metaloproteinases da Matriz/genética , Bifenilos Policlorados/toxicidade , Metamorfose Biológica/efeitos dos fármacos , Metamorfose Biológica/genética , Hormônios Tireóideos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Hidroxilação
19.
Environ Sci Pollut Res Int ; 31(23): 34675-34688, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38714614

RESUMO

The ambient air quality during COVID-19 lockdowns has been improved in many cities in the world. This study is to assess the changes in persistent organic pollutants in PM2.5 during the COVID-19 lockdown in Hanoi. Individual organic species in PM2.5 ((e.g., polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs), and organochlorine pesticides (OCPs)) were measured in an urban residential area in Hanoi from before the March 10th to April 22nd, 2020, including before the partial lockdown (BL) and the partial lockdown (PL) phases. During the PL phase, the concentration of Σ14PAHs and Σ28PCBs was reduced by 38 and 52% compared with the BL period, respectively. The diagnostic ratio method implied that the sources of PAHs within the PL phase had a less effect on traffic and industrial activities than in the BL phase. The characteristic ratio method indicated that PCBs were mixed by commercial product and combustion process in both the BL and the PL periods, however, the source of PCBs in the BL phase was influenced by municipal waste incineration more than those in the PL phase. The decreasing concentration of Σ20OCPs during the partial lockdown was attributed to the restriction of human activities during the quarantine period. The results suggested that the source of OCPs was probably derived from the usage of pesticides in current and, historical degradation or the transportation of pesticides from the soil to the atmosphere.


Assuntos
Poluentes Atmosféricos , COVID-19 , Monitoramento Ambiental , Material Particulado , Vietnã , COVID-19/epidemiologia , Material Particulado/análise , Poluentes Atmosféricos/análise , Humanos , Poluição do Ar , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , SARS-CoV-2 , Hidrocarbonetos Clorados/análise , Cidades , Poluentes Orgânicos Persistentes , Praguicidas/análise
20.
Chemosphere ; 359: 142324, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38740339

RESUMO

Seawater warming, ocean acidification and chemical pollution are the main threats to coral growth and even survival. The legacy persistent organic contaminants (POCs), such as polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), and the emerging contaminants, including polybrominated diphenyl ethers (PBDEs), dechlorane plus (DPs) and novel brominated flame retardants (NBFRs) were studied in corals from Luhuitou fringing reef in Sanya Bay and Yongle atoll in Xisha Islands, the South China Sea (SCS). Total average concentrations of ∑16PAHs, ∑23OCPs, ∑34PCBs, ∑8PBDEs, ∑2DPs and ∑5NBFRs in 20 coral species (43 samples) from the SCS were 40.7 ± 34.6, 5.20 ± 5.10, 0.197 ± 0.159, 3.30 ± 3.70, 0.041 ± 0.042 and 36.4 ± 112 ng g-1 dw, respectively. PAHs and NBFRs were the most abundant compounds and they are likely to be dangerous pollutants for future coral growth. Compared to those found in other coral reef regions, these pollutants concentrations in corals were at low to median levels. Except for PBDEs, POCs in massive Porites were significantly higher than those in branch Acropora and Pocillopora (p < 0.01), as large, closely packed corals may be beneficial for retaining more pollutant. The current study contributes valuable data on POCs, particularly for halogenated flame retardants (HFRs, including PBDEs, DPs and NBFRs), in corals from the SCS, and will improve our knowledge of the occurrence and fate of these pollutants in coral reef ecosystems.


Assuntos
Antozoários , Monitoramento Ambiental , Retardadores de Chama , Éteres Difenil Halogenados , Hidrocarbonetos Clorados , Poluentes Orgânicos Persistentes , Bifenilos Policlorados , Hidrocarbonetos Policíclicos Aromáticos , Água do Mar , Poluentes Químicos da Água , Animais , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes Químicos da Água/análise , China , Éteres Difenil Halogenados/análise , Retardadores de Chama/análise , Água do Mar/química , Bifenilos Policlorados/análise , Hidrocarbonetos Clorados/análise , Praguicidas/análise , Recifes de Corais , Oceanos e Mares
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