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3.
Development ; 148(23)2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34874451

RESUMO

The ability to derive and maintain pluripotent stem cells (PSCs) from livestock species in defined media conditions will contribute to many new research avenues, including comparative embryology and xenotransplantation. In a new paper in Development, Masaki Kinoshita, Toshihiro Kobayashi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith and colleagues describe their three-component medium, which supports long-term propagation of PSCs in the absence of feeders or serum factors. We caught up with the authors to find out more about their research and their future plans.


Assuntos
Embriologia/história , Animais , História do Século XX , História do Século XXI , Humanos , Retratos como Assunto
4.
Development ; 148(18)2021 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-34897400

RESUMO

In this Spotlight, we hear first-hand accounts from five scientists and educators who use microscopy and imaging to engage, entertain, educate and inspire new audiences with science and the field of developmental biology in particular. The 'voices' that follow each convey each authors' own personal take on why microscopy is such a powerful tool for capturing the minds, and the hearts, of scientists, students and the public alike. They discuss how microscopy and imaging can reveal new worlds, and improve our communication and understanding of developmental biology, as well as break down barriers and promote diversity for future generations of scientific researchers.


Assuntos
Microscopia , Animais , Humanos , Retratos como Assunto
6.
J Biol Chem ; 297(5): 101310, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34673029

RESUMO

This Reflections article is focused on the 5 years while I was a graduate student (1964-1969). During this period, I made some of the most significant discoveries of my career. I have written this article primarily for a protein biochemistry audience, my colleagues who shared this exciting time in science, and the many scientists over the last 50 years who have contributed to our knowledge of transcriptional machinery and their regulation. It is also written for today's graduate students, postdocs, and scientists who may not know much about the discoveries and technical advances that are now taken for granted, to show that even with methods primitive by today's standards, we were still able to make foundational advances. I also hope to provide a glimpse into how fortunate I was to be a graduate student over 50 years ago in the golden age of molecular biology.


Assuntos
RNA Polimerases Dirigidas por DNA , Proteínas de Escherichia coli , Escherichia coli , Biologia Molecular/história , Fator sigma , RNA Polimerases Dirigidas por DNA/genética , RNA Polimerases Dirigidas por DNA/história , RNA Polimerases Dirigidas por DNA/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/história , Proteínas de Escherichia coli/metabolismo , História do Século XX , História do Século XXI , Humanos , Retratos como Assunto , Fator sigma/genética , Fator sigma/história , Fator sigma/metabolismo
7.
Forensic Sci Int ; 327: 110994, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34536754

RESUMO

Genetic identification of a Slovenian prewar elite couple killed in 1944 was performed by typing autosomal and Y-chromosomal STRs, and phenotypic HIrisPlex SNPs for hair and eye color prediction were analyzed for the female skeleton using next-generation sequencing (NGS) technology. The clandestine grave containing the couple's skeletal remains was found in 2015 and only the partial remains were found. Living distant relatives could be found only for the male victim. Because of a lack of comparative reference samples, it was not possible to identify the female victim through autosomal and mitochondrial DNA typing. However, the possibility of comparison of eye and hair color with a painting exhibited in the City Museum of Ljubljana by the prominent Slovenian painter Ivana Kobilca existed. Nuclear DNA obtained from the samples was quantified using the PowerQuant System, and then STR typing was carried out with different autosomal and Y-STR kits. From 0.09-9.36 ng DNA/g of powder was obtained from teeth and bones analyzed. Complete autosomal and Y-STR profiles made it possible to identify the male skeleton via comparison with two nephews. For the female victim, predicted eye and hair color was compared to colors on the painting. Kobilca's painting confirms the genetically predicted eye and hair color. After more than seventy years, the skeletal remains of the couple were handed over to their relatives, who buried the victims with dignity in a family grave.


Assuntos
Impressões Digitais de DNA/métodos , Cor de Olho/genética , Antropologia Forense , Cor de Cabelo/genética , Sequenciamento de Nucleotídeos em Larga Escala , Análise de Sequência de DNA , Restos Mortais/química , Osso e Ossos/química , DNA/análise , Feminino , Humanos , Masculino , Retratos como Assunto , Eslovênia , Cônjuges , Dente/química , II Guerra Mundial
9.
Mol Cell ; 81(13): 2683-2685, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34214441

RESUMO

We talk to first and last authors of "Checkpoint-mediated DNA polymerase ε exonuclease activity curbing counteracts resection-driven fork collapse," Grazia Pellicanò and Rodrigo Bermejo, about their paper in this issue of Molecular Cell, the critical role of their collaborators, and the research in the Bermejo lab based in Madrid.


Assuntos
Bioquímica/história , Replicação do DNA , História do Século XXI , Retratos como Assunto
13.
Mol Cell ; 81(7): 1358-1362, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33798410

RESUMO

We talk to the authors of "Structural basis for conformational equilibrium of the catalytic spliceosome" about their paper, advances in cryo-EM that made it possible, and the influence of their late mentor Kiyoshi Nagai, to whom the paper is dedicated.


Assuntos
Microscopia Crioeletrônica/história , Splicing de RNA , Spliceossomos , História do Século XX , História do Século XXI , Humanos , Retratos como Assunto
14.
J Exp Med ; 218(5)2021 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-33861846

RESUMO

The dynamics of immune responses in asymptomatic SARS-CoV-2-infected subjects remain to be fully characterized. The work presented in this issue of JEM by Le Bert et al. (2021. J. Exp. Med.https://doi.org/10.1084/jem.20202617) sheds some light on these issues and ultimately provides some degree of confidence in the magnitude and persistence of immunity over time after asymptomatic infection with SARS-CoV-2.


Assuntos
Infecções Assintomáticas , COVID-19/imunologia , SARS-CoV-2/imunologia , Humanos , Retratos como Assunto , Fatores de Tempo
15.
Mol Cell ; 81(6): 1120-1121, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33740469

RESUMO

We talk to co-first authors Man Yu and Zhengxin Peng about their paper "Interferon- gamma induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation" (in this issue of Molecular Cell), the challenges of the project, and the collaborations and support systems that were important to overcome them.


Assuntos
Pesquisa Biomédica/história , Neoplasias , Animais , História do Século XXI , Humanos , Retratos como Assunto
16.
Mol Cell ; 81(6): 1117-1119, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33740468

RESUMO

As part of our commitment to amplifying the voices of underrepresented scientists, we are publishing the insights and experiences of a panel of underrepresented scientists. Here they discuss ways that individuals in the scientific community can begin to recognize and overcome their own racial biases. These are the personal opinions of the authors and may not reflect the views of their institutions.


Assuntos
Pesquisa Biomédica , Relações Raciais , Pesquisadores , Feminino , Humanos , Masculino , Retratos como Assunto
17.
Mol Cell ; 81(6): 1122-1125, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33740470

RESUMO

Here, we talk to first author Susanne Zellner about her paper, "Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling," and the challenges of finishing it during the pandemic. We also hear from collaborator Martina Schifferer and chat with corresponding author Christian Behrends about running his lab.


Assuntos
Pesquisa Biomédica/história , Autofagossomos , Autofagia , Feminino , História do Século XXI , Humanos , Retratos como Assunto
19.
Mol Cell ; 81(4): 639-641, 2021 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-33606970

RESUMO

As part of our commitment to amplifying the voices of underrepresented scientists, we are publishing the insights and experiences of a panel of underrepresented scientists. Here, they discuss the impact of racial bias and share strategies for addressing it. These are the personal opinions of the authors and may not reflect the views of their institutions.


Assuntos
Pesquisa Biomédica , Racismo , Humanos , Retratos como Assunto
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