Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.955
Filtrar
1.
Cell Rep ; 41(8): 111676, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36417857

RESUMO

Inflammatory monocytes (iMOs) and B cells are the main targets of the poxvirus ectromelia virus (ECTV) in the lymph nodes of mice and play distinct roles in surviving the infection. Infected and bystander iMOs control ECTV's systemic spread, preventing early death, while B cells make antibodies that eliminate ECTV. Our work demonstrates that within an infected animal that survives ECTV infection, intrinsic and bystander infection of iMOs and B cells differentially control the transcription of genes important for immune cell function and, perhaps, cell identity. Bystander cells upregulate metabolism, antigen presentation, and interferon-stimulated genes. Infected cells downregulate many cell-type-specific genes and upregulate transcripts typical of non-immune cells. Bystander (Bys) and infected (Inf) iMOs non-redundantly contribute to the cytokine milieu and the interferon response. Furthermore, we uncover how type I interferon (IFN-I) or IFN-γ signaling differentially regulates immune pathways in Inf and Bys iMOs and that, at steady state, IFN-I primes iMOs for rapid IFN-I production and antigen presentation.


Assuntos
Vírus da Ectromelia , Ectromelia Infecciosa , Interferon Tipo I , Poxviridae , Animais , Camundongos , Monócitos , Antivirais
2.
Elife ; 112022 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-36282171

RESUMO

As long suspected, poxviruses capture host genes through a reverse-transcription process now shown to be mediated by retrotransposons.


Assuntos
Poxviridae , Retroelementos , Poxviridae/genética , Vírus Vaccinia/genética , Replicação Viral
3.
Vaccine ; 40(45): 6471-6480, 2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36192275

RESUMO

Camel pox (CML) is a widespread infectious viral disease of camels that causes huge economic losses to the camel industry. In this study, a local strain of Camel pox virus (CMLV) was attenuated by 175 serial passages in Vero cells and the residual pathogenicity and infectivity were tested in naïve camels at 120, 150 and 175 passage levels. Also, the safety and immunogenicity of the 175th passage were evaluated in camels using a dose of 104.0 Tissue Culture Dose 50% (TCID50) and monitored for up to one-year post vaccination (pv) for neutralizing antibody. Seroconversion was noted at day 14 pv with neutralizing antibody titers ranging from 0.5 and 1.6 logs over the one-year of the study. Among 8 camels inoculated with the P175 strain, 4 were challenged at 12-month pv with 105.7 TCID50/ml of the original virulent CMLV and complete protection was recorded in all animals. Whole genome sequencing detected six mutations in the original CMLV strain that were not present in the attenuated 175th passage of this strain. Overall, the findings of this study indicated that the 175th passage of the CMLV was attenuated, safe and afforded protection to camels against virulent CMLV, and is therefore, a promising vaccine candidate for the prevention of CML in camels.


Assuntos
Poxviridae , Vacinas Virais , Chlorocebus aethiops , Animais , Camelus , Células Vero , Anticorpos Neutralizantes , Inoculações Seriadas , Vacinas Atenuadas
4.
Elife ; 112022 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069678

RESUMO

There is ample phylogenetic evidence that many critical virus functions, like immune evasion, evolved by the acquisition of genes from their hosts through horizontal gene transfer (HGT). However, the lack of an experimental system has prevented a mechanistic understanding of this process. We developed a model to elucidate the mechanisms of HGT into vaccinia virus, the prototypic poxvirus. All identified gene capture events showed signatures of long interspersed nuclear element-1 (LINE-1)-mediated retrotransposition, including spliced-out introns, polyadenylated tails, and target site duplications. In one case, the acquired gene integrated together with a polyadenylated host U2 small nuclear RNA. Integrations occurred across the genome, in some cases knocking out essential viral genes. These essential gene knockouts were rescued through a process of complementation by the parent virus followed by nonhomologous recombination during serial passaging to generate a single, replication-competent virus. This work links multiple evolutionary mechanisms into one adaptive cascade and identifies host retrotransposons as major drivers for virus evolution.


Assuntos
Poxviridae , Transferência Genética Horizontal , Filogenia , Poxviridae/genética , Retroelementos/genética , Vírus Vaccinia/genética
5.
Fish Shellfish Immunol ; 130: 624-634, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36126841

RESUMO

Emerging pathogen, carp edema virus (CEV) causes koi sleepy disease (KSD) in Koi and common carp causing severe mortalities worldwide. In the present study, a total of 150 fish species belonging to eight different families were sampled from the ornamental fish retailers and farms, located in Karnataka, India. The OIE protocol viz., level-I, II and III diagnoses confirmed the infection of CEV in 10 koi fish. Interestingly, other fish species belonging to different fish family including cyprinidae family were negative to CEV. Further, CEV infection was confirmed by sequencing (partial 4a gene); it showed the similarity with that of CEV reported from India and Germany strains with similarity of 97.4-99.94% and belonged to genogroup IIa. TEM analysis of purified CEV, in vivo cohabitation and tissue infection experiments confirmed the CEV infection. In addition, viral load was significantly higher (106-7 copies) in koi collected from Dakshina Kannada than of Bengaluru (103-4 copies). To understand the host-pathogen interaction, different organs such as gill, kidney, liver and spleen from naturally (CEV) infected koi were used to study the immune gene responses by using eight innate and one adaptive immune response. Results indicated that TNF-α, RohTNF-α, iNOS, IFN-γ and IL-10, and catalyze ß-2M of MHC class I pathway genes were upregulated in koi. Higher expression of immune genes during the CEV infection may have inhibited viral replication and mount an antigenic adaptive response. Similar to other viral infections, interferon-γ play an important role during poxvirus infections. Quantification of immune genes in infected fish will provide insights into the host responses and provide valuable information to devise intervention strategies to prevent and control disease due to CEV.


Assuntos
Carpas , Doenças dos Peixes , Poxviridae , Animais , Carpas/genética , Edema , Imunidade , Índia , Interferon gama , Interleucina-10 , Fator de Necrose Tumoral alfa
6.
Vopr Virusol ; 67(4): 304-309, 2022 09 11.
Artigo em Russo | MEDLINE | ID: mdl-36097711

RESUMO

INTRODUCTION: Lumpy skin disease (LSD), sheep pox and goat pox are dangerous diseases of domestic ruminants. Representatives of the genus of capripoxviruses are antigenically similar and can be used as a vaccine for three infections, as in the case of representatives of the genus of orthopoxviruses, which includes viruses of smallpox, monkeypox, and cowpox, that all belong to a single family Poxviridae. MATERIALS AND METHODS: In this study, the vaccine strain G20-LKV of the goat pox virus and the virulent strain RIBSP2019/K of the LSD virus were used. The experiments were carried out on clinically healthy cattle of the Kazakh White-headed breed, aged six to eight months. Virological and serological research methods were used in the work. RESULTS: All immunized animals that received different doses of the vaccine showed resistance to the infection challenge, without showing any clinical signs of the disease. In animals that received the lowest doses of the vaccine 15,000, 30,000 and 40,000 TCID50, no adverse events, skin and temperature reactions were observed at the injection site. Those vaccinated with high doses of the vaccine had a local reaction in the form of swelling at the site of vaccine administration. Control animals infected with a virulent virus showed clinical signs of the cattle lumpy skin disease . CONCLUSION: The vaccine, prepared based on the "G20-LKV" strain of the goat virus, is protective for cattle against infection with a virulent LSD virus at immunizing doses from 15,000 to 80,000 TCID50, which are dependent on the LSD epizootic situation in particular region.


Assuntos
Capripoxvirus , Chordopoxvirinae , Doença Nodular Cutânea , Vírus da Doença Nodular Cutânea , Infecções por Poxviridae , Poxviridae , Vacinas Virais , Animais , Capripoxvirus/genética , Bovinos , Cabras , Doença Nodular Cutânea/prevenção & controle , Vírus da Doença Nodular Cutânea/genética , Dietilamida do Ácido Lisérgico , Poxviridae/genética , Infecções por Poxviridae/prevenção & controle , Infecções por Poxviridae/veterinária , Ovinos , Vacinas Virais/genética
7.
Elife ; 112022 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069526

RESUMO

Horizontal gene transfer (HGT) provides a major source of genetic variation. Many viruses, including poxviruses, encode genes with crucial functions directly gained by gene transfer from hosts. The mechanism of transfer to poxvirus genomes is unknown. Using genome analysis and experimental screens of infected cells, we discovered a central role for Long Interspersed Nuclear Element-1 retrotransposition in HGT to virus genomes. The process recapitulates processed pseudogene generation, but with host messenger RNA directed into virus genomes. Intriguingly, hallmark features of retrotransposition appear to favor virus adaption through rapid duplication of captured host genes on arrival. Our study reveals a previously unrecognized conduit of genetic traffic with fundamental implications for the evolution of many virus classes and their hosts.


Assuntos
Poxviridae , Vírus , Evolução Molecular , Transferência Genética Horizontal , Filogenia , Poxviridae/genética , RNA Mensageiro , Vírus/genética , Retroelementos
8.
Goiânia; SES-GO; 09 set. 2022. 1-4 p. quadro.
Não convencional em Português | LILACS, Coleciona SUS, CONASS, SES-GO | ID: biblio-1392890

RESUMO

A presente nota técnica tem como objetivo orientar os profissionais de saúde em relação à condução dos casos suspeitos e/ou confirmados de Monkeypox em gestantes, puérperas e lactantes. A Monkeypox (MPX) ou Varíola M é uma doença causada pelo vírus Monkeypox do gênero Orthopoxvirus e família Poxviridae. Trata-se de uma zoonose viral cuja transmissão pode ocorrer por meio do contato desprotegido com lesões ou fluidos corporais (contato sexual, saliva, olhos, cavidade oral) e/ou materiais contaminados (roupa de cama, vestes, utensílios domésticos)


This technical note aims to guide health professionals in relation to the management of suspected and/or confirmed cases of Monkeypox in pregnant, postpartum and lactating women. Monkeypox (MPX) or Smallpox M is a disease caused by the Monkeypox virus of the genus Orthopoxvirus and family Poxviridae. It is a viral zoonosis whose transmission can occur through unprotected contact with injuries or bodily fluids (sexual contact, saliva, eyes, sinus oral) and/or contaminated materials (bedding, clothing, household items)


Assuntos
Humanos , Feminino , Gravidez , Lactente , Varíola dos Macacos/prevenção & controle , Poxviridae , Orthopoxvirus , Varíola dos Macacos/transmissão
9.
PLoS Pathog ; 18(9): e1010316, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-36103568

RESUMO

The evolutionarily successful poxviruses possess effective and diverse strategies to circumvent or overcome host defense mechanisms. Poxviruses encode many immunoregulatory proteins to evade host immunity to establish a productive infection and have unique means of inhibiting DNA sensing-dependent type 1 interferon (IFN-I) responses, a necessity given their dsDNA genome and exclusively cytoplasmic life cycle. We found that the key DNA sensing inhibition by poxvirus infection was dominant during the early stage of poxvirus infection before DNA replication. In an effort to identify the poxvirus gene products which subdue the antiviral proinflammatory responses (e.g., IFN-I response), we investigated the function of one early gene that is the known host range determinant from the highly conserved poxvirus host range C7L superfamily, myxoma virus (MYXV) M062. Host range factors are unique features of poxviruses that determine the species and cell type tropism. Almost all sequenced mammalian poxviruses retain at least one homologue of the poxvirus host range C7L superfamily. In MYXV, a rabbit-specific poxvirus, the dominant and broad-spectrum host range determinant of the C7L superfamily is the M062R gene. The M062R gene product is essential for MYXV infection in almost all cells tested from different mammalian species and specifically inhibits the function of host Sterile α Motif Domain-containing 9 (SAMD9), as M062R-null (ΔM062R) MYXV causes abortive infection in a SAMD9-dependent manner. In this study we investigated the immunostimulatory property of the ΔM062R. We found that the replication-defective ΔM062R activated host DNA sensing pathway during infection in a cGAS-dependent fashion and that knocking down SAMD9 expression attenuated proinflammatory responses. Moreover, transcriptomic analyses showed a unique feature of the host gene expression landscape that is different from the dsDNA alone-stimulated inflammatory state. This study establishes a link between the anti-neoplastic function of SAMD9 and the regulation of innate immune responses.


Assuntos
Interferon Tipo I , Myxoma virus , Infecções por Poxviridae , Poxviridae , Animais , Especificidade de Hospedeiro/genética , Humanos , Interferon Tipo I/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Macrófagos/metabolismo , Mamíferos , Monócitos/metabolismo , Myxoma virus/genética , Nucleotidiltransferases/genética , Nucleotidiltransferases/metabolismo , Poxviridae/genética , Poxviridae/metabolismo , Infecções por Poxviridae/genética , Coelhos , Transcriptoma , Vírus Vaccinia/genética , Proteínas Virais/genética , Proteínas Virais/metabolismo
10.
Viruses ; 14(9)2022 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-36146656

RESUMO

Cetacean poxviruses (CePVs) cause 'tattoo' skin lesions in small and large cetaceans worldwide. Although the disease has been known for decades, genomic data for these poxviruses are very limited, with the exception of CePV-Tursiops aduncus, which was completely sequenced in 2020. Using a newly developed pan-pox real-time PCR system targeting a conserved nucleotide sequence located within the Monkeypox virus D6R gene, we rapidly detected the CePV genome in typical skin lesions collected from two Peruvian common bottlenose dolphins (Tursiops truncatus) by-caught off Peru in 1993. Phylogenetic analyses based on the sequencing of the DNA polymerase and DNA topoisomerase genes showed that the two viruses are very closely related to each other, although the dolphins they infected pertained to different ecotypes. The poxviruses described in this study belong to CePV-1, a heterogeneous clade that infects many species of dolphins (Delphinidae) and porpoises (Phocoenidae). Among this clade, the T. truncatus CePVs from Peru were more related to the viruses infecting Delphinidae than to those detected in Phocoenidae. This is the first time that CePVs were identified in free-ranging odontocetes from the Eastern Pacific, surprisingly in 30-year-old samples. These data further suggest a close and long-standing pathogen-host co-evolution, resulting in different lineages of CePVs.


Assuntos
Golfinho Nariz-de-Garrafa , Chordopoxvirinae , Toninhas , Poxviridae , Animais , Golfinho Nariz-de-Garrafa/genética , Cetáceos , Chordopoxvirinae/genética , DNA Topoisomerases/genética , DNA Polimerase Dirigida por DNA/genética , Peru/epidemiologia , Filogenia , Toninhas/genética , Poxviridae/genética , Reação em Cadeia da Polimerase em Tempo Real
11.
Goiânia; SES-GO; ago. 2022. 2 p. ilus.
Não convencional em Português | LILACS, Coleciona SUS, CONASS, SES-GO | ID: biblio-1391617

RESUMO

A Monkeypox pertence ao mesmo vírus da família (Poxviridae) varíola. É transmitida de pessoa a pessoa. O macaco não tem participação na transmissão para humanos. Até o momento, sabe-se que na África os casos mais severos ocorreram com mais frequência em crianças. Recentemente, três crianças foram diagnosticadas com Monkeypox na cidade de São Paulo (SP) e a rápida progressão da doença indica uma alta probabilidade de mais casos nesta faixa etária em outras regiões do país


Monkeypox belongs to the same virus in the smallpox family (Poxviridae). It is transmitted from person to person. The monkey has no role in transmission to humans. So far, it is known that in Africa the most severe cases occurred more frequently in children. Recently, three children were diagnosed with Monkeypox in the city of São Paulo (SP) and the rapid progression of the disease indicates a high probability of more cases in this age group in other regions of the country


Assuntos
Humanos , Criança , Adolescente , Varíola dos Macacos/prevenção & controle , Poxviridae , Varíola dos Macacos/transmissão
12.
Viruses ; 14(7)2022 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-35891544

RESUMO

Crocodilepox virus (CRV) belongs to the Poxviridae family and mainly infects hatchling and juvenile Nile crocodiles. Most poxviruses encode inhibitors of the host antiviral protein kinase R (PKR), which is activated by viral double-stranded (ds) RNA formed during virus replication, resulting in the phosphorylation of eIF2α and the subsequent shutdown of general mRNA translation. Because CRV lacks orthologs of known poxviral PKR inhibitors, we experimentally characterized one candidate (CRV157), which contains a predicted dsRNA-binding domain. Bioinformatic analyses indicated that CRV157 evolved independently from other poxvirus PKR inhibitors. CRV157 bound to dsRNA, co-localized with PKR in the cytosol, and inhibited PKR from various species. To analyze whether CRV157 could inhibit PKR in the context of a poxvirus infection, we constructed recombinant vaccinia virus strains that contain either CRV157, or a mutant CRV157 deficient in dsRNA binding in a strain that lacks PKR inhibitors. The presence of wild-type CRV157 rescued vaccinia virus replication, while the CRV157 mutant did not. The ability of CRV157 to inhibit PKR correlated with virus replication and eIF2α phosphorylation. The independent evolution of CRV157 demonstrates that poxvirus PKR inhibitors evolved from a diverse set of ancestral genes in an example of convergent evolution.


Assuntos
Poxviridae , eIF-2 Quinase , Fator de Iniciação 2 em Eucariotos/genética , Fator de Iniciação 2 em Eucariotos/metabolismo , Fosforilação , Poxviridae/genética , Poxviridae/metabolismo , RNA de Cadeia Dupla/genética , Vírus Vaccinia/genética , Proteínas Virais/metabolismo , Replicação Viral , eIF-2 Quinase/metabolismo
13.
PLoS Pathog ; 18(7): e1010614, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35834477

RESUMO

All poxviruses contain a set of proteinaceous structures termed lateral bodies (LB) that deliver viral effector proteins into the host cytosol during virus entry. To date, the spatial proteotype of LBs remains unknown. Using the prototypic poxvirus, vaccinia virus (VACV), we employed a quantitative comparative mass spectrometry strategy to determine the poxvirus LB proteome. We identified a large population of candidate cellular proteins, the majority being mitochondrial, and 15 candidate viral LB proteins. Strikingly, one-third of these are VACV redox proteins whose LB residency could be confirmed using super-resolution microscopy. We show that VACV infection exerts an anti-oxidative effect on host cells and that artificial induction of oxidative stress impacts early and late gene expression as well as virion production. Using targeted repression and/or deletion viruses we found that deletion of individual LB-redox proteins was insufficient for host redox modulation suggesting there may be functional redundancy. In addition to defining the spatial proteotype of VACV LBs, these findings implicate poxvirus redox proteins as potential modulators of host oxidative anti-viral responses and provide a solid starting point for future investigations into the role of LB resident proteins in host immunomodulation.


Assuntos
Poxviridae , Linhagem Celular , Oxirredução , Poxviridae/genética , Poxviridae/metabolismo , Vírus Vaccinia/genética , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral
15.
Avian Dis ; 66(2): 237-242, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35838749

RESUMO

Lymphoproliferative disease virus (LPDV) is an exogenous alpharetrovirus that sporadically causes fatal lymphoid neoplasia in affected turkeys. Previous studies of wild turkeys (Meleagridis gallopavo) in the United States have demonstrated geographically widespread LPDV infection and frequent coinfection with avian poxvirus (APV) and reticuloendotheliosis virus (REV). This study was conducted to better understand health risks to Mississippi wild turkeys, including the relative importance of LPDV, APV, and REV in contributing to mortality. Thirteen wild turkeys, which died naturally or were euthanized due to illness, were submitted to Mississippi State University's Poultry Research and Diagnostic Laboratory for postmortem examinations. Birds originated from nine counties across the state over the past 5 yr. Carcasses were submitted as fresh (nonfrozen) or frozen. At autopsy, 9 of 13 turkeys had severe, proliferative cutaneous lesions on the head and neck, with diphtheritic or proliferative oral and esophageal lesions. Samples were collected for molecular diagnostic testing (LPDV and REV PCR), histopathology, and bacterial culture and isolation. External and internal parasites were preserved in formalin for identification. APV (cutaneous and/or diphtheritic forms) was diagnosed in 9 of 13 birds by identification of pathognomonic histologic lesions (including intracytoplasmic inclusion bodies). Interestingly, all birds with APV were also REV PCR positive. Furthermore, eight turkeys were positive for LPDV, and LPDV was commonly associated with coinfections with APV and REV.


El virus de la enfermedad linfoproliferativa (LPDV) es un Alfaretrovirus exógeno que esporádicamente provoca una neoplasia linfoide mortal en los pavos afectados. Estudios previos de pavos salvajes (Meleagridis gallopavo) en los Estados Unidos han demostrado que la infección por la enfermedad linfoproliferativa está geográficamente extendida y es una coinfección frecuente con el virus de la viruela aviar (APV) y el virus de la reticuloendoteliosis (REV). Este estudio se realizó para comprender mejor los riesgos para la salud de los pavos salvajes de Mississippi, incluida la importancia relativa de enfermedad linfoproliferativa, el virus de la viruela aviar y el virus de la reticuloendoteliosis en la contribución a la mortalidad. Trece pavos salvajes, que murieron naturalmente o fueron sacrificados por enfermedad, fueron enviados al Laboratorio de Investigación y Diagnóstico Avícola de la Universidad Estatal de Mississippi para exámenes post-mortem. Las aves provenían de condados de todo el estado durante los últimos cinco años. Las canales se enviaron tanto frescas (no congeladas) como congeladas. A la necropsia, 9 de 13 pavos mostraron lesiones cutáneas proliferativas graves en la cabeza y el cuello, con lesiones orales y esofágicas diftéricas o proliferativas. Se recolectaron muestras para pruebas de diagnóstico molecular (LPDV y REV PCR), histopatología y cultivo y aislamiento bacterianos. Los parásitos externos e internos se conservaron en formalina para su identificación. Se diagnosticó viruela aviar (formas cutáneas y/o diftéricas) se diagnosticó en 9 de 13 aves mediante la identificación de lesiones histológicas patognomónicas (incluidos los cuerpos de inclusión intracitoplasmáticos). Curiosamente, todas las aves con viruela aviar también fueron positivas a la presencia del virus de la reticuloendoteliosis por PCR. Además, ocho pavos fueron positivos para el virus de la enfermedad linfoproliferativa, y se asoció comúnmente con coinfecciones con viruela aviar y con el virus de la reticuloendoteliosis.


Assuntos
Alpharetrovirus , Doenças das Aves Domésticas , Poxviridae , Animais , Perus , Mississippi/epidemiologia , Doenças das Aves Domésticas/epidemiologia
16.
J Fish Dis ; 45(10): 1409-1417, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35708022

RESUMO

Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) is the causative agent of a disease (CEVD) responsible for high mortality rates in both wild and cultured common carp Cyprinus carpio. Inspection of two carp specimens from a pond with high fish mortality revealed CEV infection in both the host and its ectoparasite (Argulus foliaceus). In addition to flavobacteria, well known to be associated with gill lesions, we found that free-living eukaryotes (amoebae and ciliates) and a temporary parasite (Ichthyobodo spp.) colonizing the gills may also contribute to alterations in gill structure and/or function, either directly, through firm (Ichthyobodo) or weak (amoebae) attachment of trophozoites to the gill epithelium, or indirectly, through carriage of pathogenic bacteria. Bacterial assemblages rich in families and genera, with predominance of Cetobacterium spp. in low-intensity alteration of the gill tissue and of Flavobacterium spp. in gills with extensive necrotic lesions, were detected in gills and within the cytoplasm of associated amoebae using high-throughput sequencing. Quantitative PCR indicated F. swingsii as the prevailing flavobacterial species within amoebae from less affected gills and F. psychrophilum within amoebae from extensively affected gills. This case study suggests that eukaryotic organisms as part of the gill pathobiome may also contribute to irreversible gill lesions seen in CEVD. Emphasizing the complexity of mutual relationships between bacterial assemblages and eukaryotic co-pathogens, further studies regarding factors that trigger pathology and influence severity in the CEV-positive carp are needed.


Assuntos
Carpas , Doenças dos Peixes , Infecções por Poxviridae , Poxviridae , Animais , Edema , Doenças dos Peixes/microbiologia , Flavobacterium , Brânquias/patologia , Infecções por Poxviridae/veterinária
17.
PLoS Pathog ; 18(6): e1010612, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35727847

RESUMO

The interaction between immune cells and virus-infected targets involves multiple plasma membrane (PM) proteins. A systematic study of PM protein modulation by vaccinia virus (VACV), the paradigm of host regulation, has the potential to reveal not only novel viral immune evasion mechanisms, but also novel factors critical in host immunity. Here, >1000 PM proteins were quantified throughout VACV infection, revealing selective downregulation of known T and NK cell ligands including HLA-C, downregulation of cytokine receptors including IFNAR2, IL-6ST and IL-10RB, and rapid inhibition of expression of certain protocadherins and ephrins, candidate activating immune ligands. Downregulation of most PM proteins occurred via a proteasome-independent mechanism. Upregulated proteins included a decoy receptor for TRAIL. Twenty VACV-encoded PM proteins were identified, of which five were not recognised previously as such. Collectively, this dataset constitutes a valuable resource for future studies on antiviral immunity, host-pathogen interaction, poxvirus biology, vector-based vaccine design and oncolytic therapy.


Assuntos
Doenças Transmissíveis , Poxviridae , Vaccinia , Humanos , Evasão da Resposta Imune , Proteínas de Membrana/metabolismo , Vírus Vaccinia
18.
Goiânia; SES-GO; 23 jun. 2022. 1-5 p. ilus.
Não convencional em Português | Coleciona SUS, CONASS, SES-GO | ID: biblio-1377957

RESUMO

Monkeypox é uma rara zoonose causada pelo vírus Monkeypox que pertence ao gênero Orthopoxvirus na família Poxviridae. Deste gênero, também fazem parte os vírus da varíola humana, bovina e o vaccinia, o qual é usado na produção da vacina contra a varíola em seres humanos. Seus sintomas são semelhantes aos observados no passado em pacientes com varíola, embora clinicamente menos graves (febre, erupções cutâneas, linfonodos inflamados) (CDC, 2022a; WHO, 2022a)


Monkeypox is a rare zoonosis caused by the Monkeypox virus that belongs to the genus Orthopoxvirus in the family Poxviridae. This genus also includes human and cowpox viruses and vaccinia, which is used in the production of smallpox vaccine in human beings. humans. Its symptoms are similar to those seen in the past in smallpox patients, although clinically less severe (fever, rash, swollen lymph nodes) (CDC, 2022a; WHO, 2022a)


Assuntos
Humanos , Varíola dos Macacos/prevenção & controle , Poxviridae , Vacina Antivariólica , Orthopoxvirus
20.
Microbiol Spectr ; 10(3): e0027222, 2022 06 29.
Artigo em Inglês | MEDLINE | ID: mdl-35583360

RESUMO

Poxviruses have large DNA genomes, and they are able to infect multiple vertebrate and invertebrate animals, including humans. Despite the eradication of smallpox, poxvirus infections still remain a significant public health concern. Vaccinia virus (VV) is the prototypic member in the poxviridae family and it has been used extensively for different prophylactic applications, including the generation of vaccines against multiple infectious diseases and/or for oncolytic treatment. Many attempts have been pursued to develop novel attenuated forms of VV with improved safety profiles for their implementation as vaccines and/or vaccines vectors. We and others have previously demonstrated how RNA viruses encoding codon-deoptimized viral genes are attenuated, immunogenic and able to protect, upon a single administration, against challenge with parental viruses. In this study, we employed the same experimental approach based on the use of misrepresented codons for the generation of a recombinant (r)VV encoding a codon-deoptimized A24R gene, which is a key component of the viral RNA polymerase. Similar to our previous studies with RNA viruses, the A24R codon-deoptimized rVV (v-A24cd) was highly attenuated in vivo but able to protect, after a single intranasal dose administration, against an otherwise lethal challenge with parental VV. These results indicate that poxviruses can be effectively attenuated by synonymous codon deoptimization and open the possibility of using this methodology alone or in combination with other experimental approaches for the development of attenuated vaccines for the treatment of poxvirus infection, or to generate improved VV-based vectors. Moreover, this approach could be applied to other DNA viruses. IMPORTANCE The family poxviridae includes multiple viruses of medical and veterinary relevance, being vaccinia virus (VV) the prototypic member in the family. VV was used during the smallpox vaccination campaign to eradicate variola virus (VARV), which is considered a credible bioterrorism threat. Because of novel innovations in genetic engineering and vaccine technology, VV has gained popularity as a viral vector for the development of vaccines against several infectious diseases. Several approaches have been used to generate attenuated VV for its implementation as vaccine and/or vaccine vector. Here, we generated a rVV containing a codon-deoptimized A24R gene (v-A24cd), which encodes a key component of the viral RNA polymerase. v-A24cd was stable in culture cells and highly attenuated in vivo but able to protect against a subsequent lethal challenge with parental VV. Our findings support the use of this approach for the development of safe, stable, and protective live-attenuated VV and/or vaccine vectors.


Assuntos
Poxviridae , Varíola , Vacinas Virais , Vírus , Animais , Códon , Poxviridae/genética , Desenvolvimento de Vacinas , Vacinas Atenuadas/genética , Vírus Vaccinia/genética , Proteínas do Complexo da Replicase Viral , Vacinas Virais/genética , Vírus/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...