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1.
Med Gas Res ; 13(3): 155-158, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36571382

RESUMO

Bacterial cystitis is a common clinical problem among cats and dogs and is one of the main reasons for the administration of antimicrobials. This can cause serious damage to public and animal health, as this practice facilitates the selection of bacteria that are multidrug-resistant to antibiotics. In this context, it is urgent to understand and validate therapeutic modalities that complement antimicrobial treatment in cystitis cases. Ozone therapy has been proposed by scientists owing to the various mechanisms of action in a range of pathologies, both in human and animal medicine. This paper describes the bactericidal action of two different protocols of bladder irrigation with ozonized saline solution (59 µg/mL) in a paraplegic canine with recurrent bacterial cystitis caused by Proteus spp. In the first protocol, the bladder instillations were applied once a day for three consecutive days while in the second, successive lavages were performed throughout the day until a significant reduction in the presence of bacteria in the urine sediment. In this study, we were able to demonstrate that repeated bladder instillation within 24 hours was the most effective treatment for Proteus compared to a single instillation on successive days.


Assuntos
Cistite , Solução Salina , Animais , Cães , Humanos , Gatos , Solução Salina/uso terapêutico , Cistite/tratamento farmacológico , Cistite/microbiologia , Cistite/patologia , Resultado do Tratamento , Proteus
2.
Int J Cancer ; 151(10): 1778-1790, 2022 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-35689673

RESUMO

Proteus Donna is a randomised controlled trial aimed at prospectively evaluating screening with digital breast tomosynthesis (DBT), including interval cancer detection (ICD) and cancer detection (CD) in the analysis as a cumulative measure over subsequent screening episodes. Consenting women aged 46 to 68 attending the regional Breast Screening Service were randomly assigned to conventional digital mammography (DM, control arm) or DBT in addition to DM (DBT, study arm). At the subsequent round all participants underwent DM. Thirty-six months follow-up allowed for the identification of cancers detected in the subsequent screening and interscreening interval. Relative risk (RR) and 95% confidence interval (95% CI) were computed. Cumulative CD and Nelson-Aalen incidence were analysed over the follow-up period. Between 31 December 2014 and 31 December 2017, 43 022 women were randomised to DM and 30 844 to DBT. At baseline, CD was significantly higher (RR: 1.44, 95% CI: 1.21-1.71) in the study arm. ICD did not differ significantly between the two arms (RR: 0.92, 95% CI: 0.62-1.35). At subsequent screening with DM, the CD was lower (nearly significant) in the study arm (RR: 0.83, 95% CI: 0.65-1.06). Over the follow-up period, the cumulative CD (comprehensive of ICD) was slightly higher in the study arm (RR: 1.15, 95% CI: 1.01-1.31). The Nelson-Aalen cumulative incidence over time remained significantly higher in the study arm for approximately 24 months. Benign lesions detection was higher in the study arm at baseline and lower at subsequent tests. Outcomes are consistent with a lead time gain of DBT compared to DM, with an increase in false positives and moderate overdiagnosis.


Assuntos
Neoplasias da Mama , Detecção Precoce de Câncer , Mama/diagnóstico por imagem , Neoplasias da Mama/diagnóstico por imagem , Detecção Precoce de Câncer/métodos , Feminino , Humanos , Incidência , Mamografia/métodos , Programas de Rastreamento/métodos , Proteus
3.
Pak J Biol Sci ; 25(4): 304-312, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35638524

RESUMO

<b>Background and Objective:</b> Synergistic combinations of antimicrobial agents with different mechanisms of action are successful approaches for combating bacterial infections. This study aimed to evaluate the synergistic effect of 1-methyl ester-nigericin <b>(1)</b> and methyl 5-(hydroxymethyl) furan-2-carboxylate <b>(2)</b> against <i>Proteus</i> spp., isolates. <b>Materials and Methods:</b> The synergistic antimicrobial activity of the compounds was tested by the checkerboard method and time-kill curves. To estimate the interaction between the compounds, the Fractional Inhibitory Concentration Index (FICI) of the combination was calculated. The cytotoxic activity of the compounds in combination was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on LLC-MK2 cell lines. The reduction percentage of biofilms was obtained using the colourimetric method. <b>Results:</b> The MIC values for compounds <b>1</b> and <b>2</b> against test bacteria ranged from 39.06-78.12 µg mL<sup>1</sup> and from 78.12-156.25 µg mL<sup>1</sup>, respectively. The MIC was reduced to 1-8th as a result of the combination of compounds <b>1</b> and <b>2</b>. After 4-24 hrs of treatment with ½ MIC of compounds <b>1</b> and <b>2</b>, the killing rate (in CFU mL<sup>1</sup>) increased to a greater degree than observed with either test compound alone. The combination of compounds <b>1</b> and <b>2</b> showed a synergistic effect with FICI of 0.50 and 0.28. The synergistic combination of compounds <b>1</b> and <b>2</b> was effective on the biofilm reduction of <i>Proteus</i> <i>vulgaris</i> NP16 (85.72%) and NP47 (89.14%). <b>Conclusion:</b> This study recommends compounds <b>1</b> and <b>2</b> in combination as a potential alternative treatment agent for <i>Proteus</i> spp. infections.


Assuntos
Antibacterianos , Ésteres , Antibacterianos/farmacologia , Sinergismo Farmacológico , Ésteres/farmacologia , Furanos , Testes de Sensibilidade Microbiana , Nigericina , Proteus
4.
Mymensingh Med J ; 31(2): 289-294, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35383740

RESUMO

Proteus is considered as one of the major opportunistic pathogens liable for nosocomial infections and acquired several resistances to a wide range of antimicrobials such as aminoglycosides. The most common mechanism of aminoglycoside resistance is the inactivation of drugs by modifying enzymes. So, this cross-sectional study was undertaken to investigate the occurrence of aminoglycoside resistance and identify aminoglycoside modifying enzyme (AME) genes among clinical isolates of aminoglycoside resistant Proteus spp. A total of 40 Proteusmirabilis and Proteus vulgaris were isolated in the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh from July 2018 to June 2019 of 500 wound swab & pus, urine and blood samples. Disk diffusion test was performed by modified Kirby Bauer method. Minimum inhibitory concentration (MIC) of amikacin was determined by agar dilution method. PCR was used to detect aac(3)-Ia, aac(6')-Ib, ant(4')-IIa, ant(2'')-Ia a and aph(3'')-Ib AMEs genes among aminoglycoside resistant Proteus spp. Sequencing of aac(6')-Ib gene was performed to identify aac(6')-Ib-cr variant. Thirty-two (80%) aminoglycoside resistant isolates were detected during disk-diffusion technique. The marked increase in MIC was observed between 256 - ≥2048µg/ml to amikacin. The most prevalent AME-genes were aac(6')-Ib (37.5%), ant(2'')-Iaa (21.86) followed by ant(4')-IIa(12.5%), aph(3'')-Ib (12.5%) andaac(3)-Ia (9.38%). The most frequent combination was aac(6')-Ib + aac(3)-Ia+ant(2'')-Iaa and aac(6')-Ib + ant(4')-IIa + aph(3'')-Ib(2 strains) followed by aac(6')-Ib + aac(3)-Ia(1 strain). Sequencing of aac(6')-Ib gene in this study did not harbor aac(6')-Ib-cr variant gene. The results of this study provide insight into the presence of high AME-genes among Proteus spp. in Bangladesh.


Assuntos
Aminoglicosídeos , Farmacorresistência Bacteriana , Aminoglicosídeos/farmacologia , Antibacterianos/farmacologia , Bangladesh , Estudos Transversais , Farmacorresistência Bacteriana/genética , Humanos , Testes de Sensibilidade Microbiana , Proteus/genética , Centros de Atenção Terciária
5.
Sci Rep ; 12(1): 5437, 2022 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-35361872

RESUMO

Excessive ammonium blood concentration causes many serious neurological complications. The medications currently used are not very effective. To remove ammonium from the blood, erythrocyte-bioreactors containing enzymes that processing ammonium have been proposed. The most promising bioreactor contained co-encapsulated glutamate dehydrogenase (GDH) and alanine aminotransferase (ALT). However, a low encapsulation of a commonly used bovine liver GDH (due to high aggregation), makes clinical use of such bioreactors impossible. In this study, new bioreactors containing ALT and non-aggregating GDH at higher loading were first produced using the flow dialysis method and the new bacterial GDH enzyme from Proteus sp. The efficacy of these erythrocyte-bioreactors and their properties (hemolysis, osmotic fragility, intracellular and extracellular activity of included enzymes, erythrocyte indices, and filterability) were studied and compared with native cells during 1-week storage. The ammonium removal rate in vitro by such erythrocyte-bioreactors increased linearly with an increase in encapsulated GDH activity. Alanine in vitro increased in accordance with ammonium consumption, which indicated the joint functioning of both included enzymes. Thus, novel bioreactors for ammonium removal containing GDH from Proteus sp. are promising for clinical use, since they have a more efficient GDH encapsulation and their properties are not inferior to previously obtained erythrocyte-bioreactors.


Assuntos
Compostos de Amônio , Glutamato Desidrogenase , Alanina Transaminase , Animais , Reatores Biológicos , Bovinos , Eritrócitos , Proteus , Suínos
6.
Huan Jing Ke Xue ; 43(3): 1455-1462, 2022 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-35258209

RESUMO

Currently, research on the effects of microplastics (MPs) in biofilms has mainly been focused on the mature biofilm communities, with a lack of sufficient details on the influence on different development stages of biofilms. Proteus and 1 µm polystyrene microplastics (PS-MPs), which are widely found in the environment, were selected as the research objects to explore the effects of microplastics on biofilms at different developmental stages. In our study, the effects of PS-MPs on biofilm biomass, extracellular polymer composition(EPS), and extracellular enzyme activity were investigated using an exposure test. Our results showed that the effect of PS-MPs on biofilms at different stages was similar, but the effect was significantly reduced with the development of biofilms. Biofilms at different development stages had different sensitivities to microplastics. In the reversible attachment stage, the no observed effect concentration (NOEC) of EPS composition, reactive oxygen species (ROS) production, and extracellular enzyme activity were significantly lower than those in other stages; however, the NOEC of total antioxidant capacity (T-AOC) and lactate dehydrogenase (LDH) activity were similar. This may be the result of ROS-mediated protein oxidation, which can be reduced but not completely eliminated by EPS in other stages of biofilm. This indicates that PS-MPs has a low toxic effect on biofilm.


Assuntos
Microplásticos , Poluentes Químicos da Água , Biofilmes , Microplásticos/toxicidade , Plásticos , Poliestirenos , Proteus , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
7.
J Pediatr Orthop ; 42(5): 280-284, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35153286

RESUMO

PURPOSE: Surgical site infection (SSI) rates in pediatric spinal deformity surgery for cerebral palsy (CP) patients are higher than that in idiopathic scoliosis. The use of vancomycin powder is associated with decreased risk of SSI in neuromuscular patients. Prior studies in adult and pediatric early-onset scoliosis patients have shown that vancomycin powder alters microbacterial profile in patients that develop SSI. However, the effects of topical vancomycin powder on microbiology in spinal deformity surgery for CP patients has not been studied. METHODS: An international multicenter database of CP neuromuscular scoliosis patients was used in this retrospective cohort study. All patients that underwent posterior spinal instrumented fusion for CP neuromuscular scoliosis from 2008 to 2019 were queried, and 50 cases complicated by postoperative SSI were identified. Intraoperative antibiotic details were documented in 49 cases (98.0%). Microbiology details were documented in 45 cases (91.8%). Microbiology for patients that received topical vancomycin powder were compared with patients that did not. A multivariate regression model was used to control for potential confounders. RESULTS: There were 45 patients included in this study. There were 27 males (60.0%) and 18 females (40.0%). Mean age at surgery was 14.8±2.4 years. There were 24 patients that received topical vancomycin powder (53.3%). The mean time from index surgery to SSI was 4.3±11.3 months.On univariate analysis of microbiology cultures by vancomycin powder cohort, there were no significant differences in culture types. Proteus spp. trended on significance with association with vancomycin powder use (P=0.078). When controlling for potential confounders on multivariate analysis, intraoperative topical vancomycin powder was associated with increased risk for proteus infection (adjusted odds ratio: 262.900, 95% confidence interval: 1.806-38,267.121, P=0.028). DISCUSSION: In CP patients undergoing pediatric spinal deformity surgery, the use of vancomycin powder was independently associated with increased risk for proteus infections. Further study into antibiotic regimens for spinal deformity surgery in the CP population should be performed. LEVEL OF EVIDENCE: Level III-retrospective cohort study.


Assuntos
Paralisia Cerebral , Doenças do Tecido Conjuntivo , Escoliose , Fusão Vertebral , Adulto , Antibacterianos/uso terapêutico , Antibioticoprofilaxia , Paralisia Cerebral/complicações , Paralisia Cerebral/tratamento farmacológico , Paralisia Cerebral/cirurgia , Criança , Doenças do Tecido Conjuntivo/complicações , Feminino , Humanos , Masculino , Pós/uso terapêutico , Proteus , Estudos Retrospectivos , Escoliose/etiologia , Fusão Vertebral/efeitos adversos , Infecção da Ferida Cirúrgica/tratamento farmacológico , Infecção da Ferida Cirúrgica/epidemiologia , Infecção da Ferida Cirúrgica/etiologia , Vancomicina/uso terapêutico
8.
J Glob Antimicrob Resist ; 29: 124-130, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35218939

RESUMO

OBJECTIVES: The prevalence of tet(X) genes threatens the clinical use of last-line tigecycline. The tet(X6) gene has been reported in Proteus strains, but its genetic context is rarely reported. This study aimed to investigate the prevalence and genetic contexts of tet(X6) gene in Proteus spp. METHODS: A tet(X6) variant-bearing P. terrae subsp. cibarius strain was subjected to susceptibility testing, determination of growth curves, scanning electron microscopy, transmission electron microscopy and whole-genome sequencing (WGS). The genomic contexts of the tet(X6)-positive strain were analysed by sequence comparison and annotation. RESULTS: ZJ19PC, a P. terrae subsp. cibarius strain harbouring the tet(X6) variant, was isolated from 20 cecum samples collected in Zhejiang, China. The chromosome size of ZJ19PC was 3 952 084 bp; the GC content was 38.2%; and hugA, sul2, tet(H), floR, dfra1, aadA1, aac(3)-IV and aph(4)-la were found in addition to the tet(X6) variant. Proteus spp. could be classified into three groups based on the tet(X6) gene contexts. Strain ZJ19PC belongs to group 1 (sra-sul2-ISCR2-floR-ISCR2-floR-ISCR2- tet(X6) variant-tnpA-ISEc59-aph(4)-la-aac(3)-Iva-IS26), and this region of group 1 was inserted between modA and guaA. The common antimicrobial resistance (AMR) genes of the three types of AMR gene islands were sul2, floR, tet(X6) and aac(3). The tet(X6) gene contexts and SNP tree showed that ZJ19PC was homologous to HNCF44W and HNCF43W, which indicated that these strains may be clonally transmitted. CONCLUSION: This study analysed the genetic contexts of the tet(X6) gene in Proteus spp. and highlighted the significance of monitoring tigecycline-resistant P. terrae subsp. cibarius.


Assuntos
Antibacterianos , Proteus , Animais , Antibacterianos/farmacologia , Ceco , Tigeciclina
9.
Clin Trials ; 19(3): 277-284, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35094586

RESUMO

BACKGROUND: The assessment of patient-reported outcomes in clinical trials has enormous potential to promote patient-centred care, but for this potential to be realized, the patient-reported outcomes must be captured effectively and communicated clearly. Over the past decade, methodologic tools have been developed to inform the design, analysis, reporting, and interpretation of patient-reported outcome data from clinical trials. We formed the PROTEUS-Trials Consortium (Patient-Reported Outcomes Tools: Engaging Users and Stakeholders) to disseminate and implement these methodologic tools. METHODS: PROTEUS-Trials are engaging with patient, clinician, research, and regulatory stakeholders from 27 organizations in the United States, Canada, Australia, the United Kingdom, and Europe to develop both organization-specific and cross-cutting strategies for implementing and disseminating the methodologic tools. Guided by the Knowledge-to-Action framework, we conducted consortium-wide webinars and meetings, as well as individual calls with participating organizations, to develop a workplan, which we are currently executing. RESULTS: Six methodologic tools serve as the foundation for PROTEUS-Trials dissemination and implementation efforts: the Standard Protocol Items: Recommendations for Interventional Trials-patient-reported outcome extension for writing protocols with patient-reported outcomes, the International Society for Quality of Life Research Minimum Standards for selecting a patient-reported outcome measure, Setting International Standards in Analysing Patient-Reported Outcomes and Quality of Life Endpoints Data Consortium recommendations for patient-reported outcome data analysis, the Consolidated Standards for Reporting of Trials-patient-reported outcome extension for reporting clinical trials with patient-reported outcomes, recommendations for the graphic display of patient-reported outcome data, and a Clinician's Checklist for reading and using an article about patient-reported outcomes. The PROTEUS-Trials website (www.TheProteusConsortium.org) serves as a central repository for the methodologic tools and associated resources. To date, we have developed (1) a roadmap to visually display where each of the six methodologic tools applies along the clinical trial trajectory, (2) web tutorials that provide guidance on the methodologic tools at different levels of detail, (3) checklists to provide brief summaries of each tool's recommendations, (4) a handbook to provide a self-guided approach to learning about the tools and recommendations, and (5) publications that address key topics related to patient-reported outcomes in clinical trials. We are also conducting organization-specific activities, including meetings, presentations, workshops, and webinars to publicize the existence of the methodologic tools and the PROTEUS-Trials resources. Work to develop communications strategies to ensure that PROTEUS-Trials reach key audiences with relevant information about patient-reported outcomes in clinical trials and PROTEUS-Trials is ongoing. DISCUSSION: The PROTEUS-Trials Consortium aims to help researchers generate patient-reported outcome data from clinical trials to (1) enable investigators, regulators, and policy-makers to take the patient perspective into account when conducting research and making decisions; (2) help patients understand treatment options and make treatment decisions; and (3) inform clinicians' discussions with patients regarding treatment options. In these ways, the PROTEUS Consortium promotes patient-centred research and care.


Assuntos
Medidas de Resultados Relatados pelo Paciente , Qualidade de Vida , Ensaios Clínicos como Assunto , Tomada de Decisões , Humanos , Proteus , Projetos de Pesquisa , Estados Unidos
10.
J Econ Entomol ; 115(2): 573-581, 2022 04 13.
Artigo em Inglês | MEDLINE | ID: mdl-35078239

RESUMO

Proteus, matrine, and pyridalyl were tested in the laboratory for their effects upon Orius laevigatus (Fieber), which is a polyphagous predator used for IPM programs of Frankliniella occidentalis (Pergande). Against female adults, the most toxic insecticide was Proteus (LC50 = 44.3 µl L-1), followed by pyridalyl (LC50 = 83.8 µl L-1) and matrine (LC50 = 102.7 µl L-1). The mortality of female adults was checked 24, 48, 72, and 96 h after exposure to 14-d residues of the treatments on strawberry leaves. The residual of Proteus was less toxic; the remaining products caused the lowest mortality at different times after exposure. Sublethal treatments (LC25) significantly prolonged the developmental duration of total immature stages from 17.6 d in control to 21.6 and 20.0 d in Proteus and pyridalyl treatments, respectively. Also, the fecundity of O. laevigatus treated with Proteus, pyridalyl, and matrine decreased to 58.8%, 75.6%, and 96.7%, respectively, in comparison to the control. Compared with the control population (0.118 d-1), the intrinsic rate of increase (r) of F1 generation decreased by 0.053, 0.095, and 0.110 d-1 in Proteus, pyridalyl, and matrine treatments, respectively. The consumption rate of control bugs reached 14.0 thrips during 24 h. The adults fed on Proteus treatment had the lowest consumption rate in this period (9.4 preys). Overall, matrine proved to be harmless with reproductive capacity and r similar to what was recorded in control bugs. We concluded that matrine can be used as an alternative for the synthetic insecticide to integrate with O. laevigatus.


Assuntos
Hemípteros , Heterópteros , Inseticidas , Tisanópteros , Alcaloides , Animais , Feminino , Inseticidas/toxicidade , Éteres Fenílicos , Proteidae , Proteus , Quinolizinas
11.
Sci Rep ; 12(1): 1432, 2022 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-35082322

RESUMO

Faecal (FM) and colon mucosal associated microbiota (MAM) were studied in a model of colorectal cancer (CRC), the Apc-mutated Pirc rats, and in age-paired wt F344 rats. Principal Coordinates Analysis indicated that samples' distribution was driven by age, with samples of young rats (1 month old; without tumours) separated from older ones (11-month-old; bearing tumours). Diversity analysis showed significant differences between FM and MAM in older Pirc rats, and between MAM of both Pirc and wt rats and the tumour microbiota, enriched in Enterococcus, Escherichia/Shigella, Proteus and Bifidobacteriaceae. In young animals, Pirc FM was enriched in the genus Delftia, while wt FM was enriched in Lactobacillus and Streptococcus. Some CRC biomarkers and faecal short chain fatty acids (SCFAs) were also measured. Colon proliferation and DClK1 expression, a pro-survival mucosal marker, were higher in Pirc than in wt rats, while the mucin MUC2, was lower in Pirc rats. Branched SCFAs were higher in Pirc than in wt animals. By Spearman analysis CRC biomarkers correlated with FM (in both young and old rats) and with MAM (in young rats), suggesting a specific relationship between the gut microbiota profile and these functional mucosal parameters deserving further investigation.


Assuntos
Biomarcadores Tumorais/genética , Carcinogênese/genética , Colo/microbiologia , Neoplasias do Colo/genética , Quinases Semelhantes a Duplacortina/genética , Mucina-2/genética , Fatores Etários , Animais , Bifidobacterium/crescimento & desenvolvimento , Bifidobacterium/isolamento & purificação , Biomarcadores Tumorais/metabolismo , Carcinogênese/metabolismo , Carcinogênese/patologia , Colo/metabolismo , Neoplasias do Colo/metabolismo , Neoplasias do Colo/microbiologia , Neoplasias do Colo/patologia , Modelos Animais de Doenças , Quinases Semelhantes a Duplacortina/metabolismo , Enterococcus/crescimento & desenvolvimento , Enterococcus/isolamento & purificação , Escherichia/crescimento & desenvolvimento , Escherichia/isolamento & purificação , Ácidos Graxos Voláteis/metabolismo , Fezes/microbiologia , Regulação da Expressão Gênica , Lactobacillus/crescimento & desenvolvimento , Lactobacillus/isolamento & purificação , Masculino , Mucina-2/metabolismo , Análise de Componente Principal , Proteus/crescimento & desenvolvimento , Proteus/isolamento & purificação , Ratos , Ratos Endogâmicos F344 , Shigella/crescimento & desenvolvimento , Shigella/isolamento & purificação , Streptococcus/crescimento & desenvolvimento , Streptococcus/isolamento & purificação
12.
Mymensingh Med J ; 31(1): 31-36, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34999676

RESUMO

Plasmid mediated quinolone resistance (PMQR) has been revealed to play not only a significant role in quinolone resistance but also this drug resistance can spread from one bacterium to another. There is limited data regarding the prevalence of PMQR are available from Bangladesh. So, the aim of this study was to detect the prevalence of qnr and aac(6')-Ib-cr genes among clinical isolates of ciprofloxacin resistant Proteus spp. This cross-sectional study was carried out in the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh from July 2018 to June 2019. Fourty (40) Proteus spp. was isolated from 300 culture positive samples. Proteus mirabilis and Proteus vulgaris were identified by culture and biochemical test. Antibiotic susceptibility was performed by disc-diffusion technique. Quinolone resistance genes (qnrA, qnrB, qnrC, qnrD, qnrS and aac(6')-1b-cr) among ciprofloxacin resistant Proteus spp. were detected by PCR. Thirty (75%) ciprofloxacin resistant isolates were detected during disk-diffusion technique. Among them, quinolone resistance genes were found positive 11(36.67%) for aac(6')-Ib-cr, 6(20%) for qnrA, 5(16.67%) for qnrD, 4(13.33%) for qnrS and 3(10%) for qnrB genes. Co-existance of qnrA + aac(6')-Ib-cr and qnrD + qnrS were found in 3(10%) wound swab & pus and urine samples respectively followed by qnrA + qnrB in 2(6.67%) wound swab and pus and qnrA+qnrS in 1(3.33%) urine sample. The results of this study showed presence of high (66.67%) percentage of PMQR genes as well as high (30%) rate of co-carriage of the two genes among Proteus spp. isolates. The incidence of PMQR genes was found to be high which could be due to the increased prescription of fluoroquinolones. Thus, there is a need for rational usage of fluoroquinolones.


Assuntos
Farmacorresistência Bacteriana , Proteínas de Escherichia coli , Antibacterianos/farmacologia , Bangladesh/epidemiologia , Estudos Transversais , Escherichia coli , Humanos , Testes de Sensibilidade Microbiana , Prevalência , Proteus , Centros de Atenção Terciária
13.
Health Commun ; 37(2): 222-229, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-33054371

RESUMO

This study combined user-avatar similarity and Proteus effect predictions to incentivize physical activity. 305 participants ran while wearing accelerometers and a heart rate monitor. They were randomly assigned to onscreen motion-capturing avatars displaying either participant or stranger faces dressed in sports or formal clothes. Participants assigned to avatars displaying their own face showed increased cardiac frequency compared with those exposed to avatars with a stranger's face. Relative to the remaining conditions, participants assigned to avatars with their own face also wearing sports clothes showed increased cardiac frequency but participants assigned to avatars with a stranger's face wearing formal clothes showed decreased cardiac frequency. The results imply that user-avatar similarity and the Proteus effect can be harnessed to influence physical activity.


Assuntos
Exercício Físico , Interface Usuário-Computador , Humanos , Proteus
14.
Braz. j. biol ; 82: e233523, 2022. tab
Artigo em Inglês | LILACS | ID: biblio-1153470

RESUMO

Abstract Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.


Resumo Estudos microbiológicos da sanidade de psitacídeos que serão reintroduzidos são importantes para avaliar se esses animais atuam como portadores de agentes patogênicos para outros animais e humanos. A espectrometria de massa por ionização/dessorção de matriz assistida por laser/tempo de vôo (MALDI-TOF MS) é um método mais rápido e preciso para identificar bactérias na comparação com métodos convencionais de microbiologia. O objetivo deste estudo foi avaliar o estado de saúde de psitacídeos cativos, identificando bactérias Gram-negativas da microbiota fecal por MALDI -TOF MS. Os resultados indicaram alta frequência de bactérias Gram-negativas nas fezes (96,5%), principalmente da família Enterobacteriaceae (88,7%). As mais prevalentes foram Escherichia coli (39,0%), Proteus vulgaris (12,2%), Klebsiella spp. (12,1%) e Raoultella ornithinolytica (8,7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. e Escherichia hermannii foram isolados com menor frequência. Todos esses agentes são potencialmente patogênicos para os papagaios e podem causar infecções sistêmicas em outros animais e seres humanos. Esses achados reforçam que o MALDI- TOF MS é um método rápido e preciso de identificação do microrganismo e avaliação do estado de saúde dos psitacídeos, fornecendo dados relevantes para auxiliar na tomada de decisões sobre os protocolos sanitários em centros de triagem de animais selvagens e sobre a possibilidade de reintrodução futura.


Assuntos
Humanos , Animais , Psittaciformes , Bactérias Gram-Negativas , Proteus , Providencia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Enterobacteriaceae
15.
Mar Pollut Bull ; 172: 112872, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34454388

RESUMO

Halogenated natural products (HNPs) were identified from organic extracts of the marine sponge Hyrtios proteus from The Bahamas using gas chromatography with electron capture negative ion mass spectrometry and non-targeted gas chromatography with electron ionization mass spectrometry. The HNPs found have similar properties to anthropogenic persistent organic pollutants (POPs). Two ortho-methoxy brominated diphenyl ethers (MeO-BDEs) 2'-MeO-BDE 68 and 6-MeO-BDE 47 were the most abundant compounds. Fourteen other MeO-BDEs were detected along with several polybrominated dibenzo-p-dioxins (PBDDs) (1,3,7-triBDD, 1,3,6,8-tetraBDD and 1,3,7,9-tetraBDD) and MeO-PBDDs. Further analysis of a higher trophic level octopus (Octopus maya) from the same FAO fishing area showed that the major HNPs detected in Hyrtios proteus were also predominant. Moreover, HNPs were more than 30-fold higher in abundance than the major POPs in the octopus, i.e., polychlorinated biphenyls. Hence, Caribbean marine organisms, including those potentially used for food, harbor relatively high concentrations of HNPs.


Assuntos
Dioxinas , Bifenil Polibromatos , Poríferos , Animais , Bahamas , Dioxinas/análise , Cromatografia Gasosa-Espectrometria de Massas , Éteres Difenil Halogenados/análise , Bifenil Polibromatos/análise , Proteus
16.
Environ Entomol ; 50(5): 1137-1144, 2021 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-34279589

RESUMO

The western flower thrips (WFT), Frankliniella occidentalis (Pergande) is one of the most harmful pests of crops in greenhouses and fields. Considering the need for studies that introduce new insecticides for control of the WFT, the leaf dip method was carried out to study the acute toxicity of Proteus, matrine, and pyridalyl to adult thrips, and life tables were constructed to assess the impacts of sublethal concentrations (LC25) of these insecticides on the development and reproduction of the F1 generation. Bioassays showed that the toxicity of matrine (LC50: 45.9 µl ml-1) and Proteus (LC50: 54.5 µl ml-1) was higher than pyridalyl (LC50: 176.5 µl ml-1). At LC25 concentration, both Proteus and matrine prolonged the development period and reduced the survival rate of eggs, larval stages, and pupae in the F1 generation. Also, the adults' longevity, oviposition duration, and the cumulative number of eggs laid per female (fecundity) were decreased significantly. Sublethal concentrations of Proteus and matrine inhibited the population growth rate relative to the control based on the predicted number of offspring. The lowest net reproductive rate (R0), intrinsic rate of increase (r), and finite rate of increase (λ) were estimated for Proteus (7.02 offspring/individual, 0. 0838 d-1, and 1. 08 d-1, respectively). In contrast, the WFT F1 generation that resulted from parent adults treated with pyridalyl was neither affected in their developmental time, nor fecundity, or the intrinsic rate of increase. According to our findings, all tested insecticides, especially Proteus, showed good potential for use in integrated pest management strategies against F. occidentalis.


Assuntos
Inseticidas , Tisanópteros , Alcaloides , Animais , Feminino , Inseticidas/toxicidade , Óvulo , Éteres Fenílicos , Proteus , Quinolizinas
17.
Microb Drug Resist ; 27(12): 1603-1615, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33956535

RESUMO

In this study, we report a high incidence of New Delhi metallo-ß-lactamase (NDM)-producing and ampicillin-catabolizing bacteria within carbapenem-resistant bacterial populations in the waters of two important rivers, Mahananda and Karala, bisecting two most populous towns, Siliguri and Jalpaiguri, respectively, in the northern West Bengal, India. Isolates producing NDM belonged to four genera, Acinetobacter, Escherichia, Proteus, and Pseudomonas; among which few were phylogenetically determined as putatively novel species. Class 1 integrons with the frequent presence of aadA and aac(6')-Ib gene cassettes in 50% of NDM-bearing isolates are indicative of possible selective pressures generated out of unregulated use of streptomycin, in agriculture practiced by the cultivators and tea planters living in locales drained by these two rivers, in their up- and downstream, and amikacin in the most crowded government-sponsored "sadar" and district hospitals of Siliguri and Jalpaiguri. NDM-delivering bacteria in rivers have genuine consequences for city inhabitants who are dependent on public water and sanitation facilities. Standard reconnaissance of antibiotic resistance, consolidating ecological sampling just as the assessment of clinical isolates, should be set up as a need.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/genética , Rios/microbiologia , beta-Lactamases/genética , Acinetobacter/efeitos dos fármacos , Escherichia/efeitos dos fármacos , Genes Bacterianos , Índia , Integrons , Testes de Sensibilidade Microbiana , Proteus/efeitos dos fármacos , Pseudomonas/efeitos dos fármacos
18.
Mymensingh Med J ; 30(2): 355-361, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33830114

RESUMO

Different Proteus species are encountered in human infections and may vary with the type of infections they cause. So, the present study was conducted to detect species of Proteus by PCR and RFLP along with their antibiotic resistance pattern. This cross-sectional study was conducted in the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh, from July 2018 to June 2019. A total of 500 wound swab and pus, urine and blood samples were tested for bacterial pathogens. Proteus spp. were identified and differentiated by biochemical test, PCR and RFLP. Antibiotic susceptibility was performed by disc-diffusion technique. Fourty Proteus spp. was isolated from 300 culture positive samples, giving 13.33% prevalence of Proteus infections. Proteus mirabilis and Proteus vulgaris were identified by culture, biochemical test, PCR and RFLP. The results were similar by both methods (biochemical tests and PCR). RFLP of 16S rRNA fragments digested with HaeIII revealed that P. mirabilis consisted of two bands at approximately 110 and 190 bp and P. vulgaris consisted of three bands at approximately 100, 180 and 220 bp. The proportion (80%) of P. mirabilis was more than P. vulgaris. Highest proportion (77.5%) of Proteus spp. was isolated from wound swab and pus followed by urine samples. A significant proportion of Proteus spp. was multidrug resistant (90%) and extensively drug resistant (37.5%). Fosfomycin was found the most sensitive drug followed by imipenem. This study provided an insight into antibiotic resistance pattern of Proteus spp. and showed high level resistance towards commonly used antimicrobial agents. PCR and RFLP may be suitable method to identify and differentiate species of Proteus and to treat them accordingly.


Assuntos
Antibacterianos , Proteus , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bangladesh , Estudos Transversais , Resistência Microbiana a Medicamentos , Humanos , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Proteus/genética , RNA Ribossômico 16S
19.
Braz J Biol ; 82: e233523, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33787713

RESUMO

Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.


Assuntos
Bactérias Gram-Negativas , Psittaciformes , Animais , Enterobacteriaceae , Humanos , Proteus , Providencia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
20.
Open Vet J ; 10(4): 377-383, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33614432

RESUMO

Background: Feline otitis externa is a dermatological disorder with a multifactorial complex etiology. Aim: This study aimed to investigate the prevalence of different etiological agents, particularly the parasitic and bacterial, responsible for the cases of feline otitis externa in Tripoli, Libya, and to assess the antimicrobial susceptibility of the bacterial isolates from those cases. Methods: Cerumen and otic discharges of the suspected cats were collected for parasite detection and bacterial culture. Kirby-Bauer's disk diffusion method was used for antimicrobial susceptibility testing. Results: The results showed that otodectic mites and bacterial causes were equally the most prevalent in those cases, with a prevalence of 47.1% each. Otodectes cynotis infestation was more frequently bilateral and severe. Staphylococcus spp. were the most prevalent among bacterial causes (75%), followed by Proteus spp. (16.6%) and Pseudomonas spp. (8.4%). Norfloxacin and gentamicin were the most effective antimicrobials against bacterial isolates, as they were effective against 83.3% and 70.8% of isolates, respectively. Conclusion: Otodectes cynotis infestation and staphylococcal infections constituted the most common etiology of feline otitis externa in Tripoli, Libya, and norfloxacin represented a cogent antibacterial for the treatment of otitis externa.


Assuntos
Antibacterianos/farmacologia , Doenças do Gato/epidemiologia , Infestações por Ácaros/veterinária , Otite Externa/veterinária , Infecções por Proteus/veterinária , Infecções por Pseudomonas/veterinária , Infecções Estafilocócicas/veterinária , Animais , Doenças do Gato/microbiologia , Doenças do Gato/parasitologia , Gatos , Feminino , Gentamicinas/farmacologia , Líbia/epidemiologia , Masculino , Infestações por Ácaros/epidemiologia , Infestações por Ácaros/parasitologia , Ácaros/fisiologia , Norfloxacino/farmacologia , Otite Externa/epidemiologia , Otite Externa/microbiologia , Otite Externa/parasitologia , Prevalência , Proteus/efeitos dos fármacos , Infecções por Proteus/epidemiologia , Infecções por Proteus/microbiologia , Pseudomonas/efeitos dos fármacos , Infecções por Pseudomonas/epidemiologia , Infecções por Pseudomonas/microbiologia , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos
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