Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 496
Filtrar
1.
An Acad Bras Cienc ; 93(suppl 4): e20210047, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34730621

RESUMO

The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties. In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions. The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides. The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations. Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study. Polysaccharides from the biomass and culture broth were extracted with hot water. One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type. Differences were also found in the elution profiles from HPSEC and NMR analyses. From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions. To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.


Assuntos
Schizophyllum , Parede Celular , Mutação/genética , Fenótipo , Polissacarídeos , Schizophyllum/genética
2.
BMC Infect Dis ; 21(1): 1052, 2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34627188

RESUMO

BACKGROUND: Schizophyllum commune is a basidiomycete that lives in the environment and can cause infections, mainly those of the respiratory system. Although S. commune is increasingly reported as a cause of allergic bronchopulmonary mycosis and sinusitis, cases of fungal ball formation are extremely uncommon. Identification of S. commune is difficult using routine mycological diagnostic methods, and in clinically suspicious cases, internal transcribed spacer sequencing should be used for diagnosis. Here, we report a first case of lung cancer with a fungal ball formation of S. commune, confirmed by analyzing the internal transcribed spacer. CASE PRESENTATION: A 76-year-old man with diabetes and hypertension was admitted to the hospital with a chief complaint of hemosputum, which he had for about 19 months. A computed tomography image of the patient's chest showed a cavity and internal nodule in the left upper lobe of his lung. A left upper lobectomy was performed, and histopathological examination revealed squamous cell carcinoma of the lung and a fungal ball. The isolate from the surgical specimen was identified as S. commune by analyzing the internal transcribed spacer. The patient had no recurrence of the infection during 5 months of follow-up. CONCLUSIONS: Only three cases of lung fungal balls caused by S. commune have been previously reported, and this is the first case of lung cancer cavity with a fungal ball formation. In cases of fungal ball formation in the lung, S. commune should be considered a possible causative microorganism.


Assuntos
Aspergilose Pulmonar Invasiva , Neoplasias Pulmonares , Schizophyllum , Tuberculose Pulmonar , Idoso , Humanos , Masculino , Recidiva Local de Neoplasia , Schizophyllum/genética
3.
Sci Rep ; 11(1): 8178, 2021 04 14.
Artigo em Inglês | MEDLINE | ID: mdl-33854169

RESUMO

Mushroom formation represents the most complex multicellular development in fungi. In the model mushroom Schizophyllum commune, comparative genomics and transcriptomics have previously resulted in a regulatory model of mushroom development. However, little is known about the role of epigenetic regulation. We used chromatin immunoprecipitation sequencing (ChIP-Seq) to determine the distribution of dimethylation of lysine 4 on histone H3 (H3K4me2), a mark for transcriptionally active genes, during monokaryotic and dikaryotic development. We identified a total of 6032 and 5889 sites during monokaryotic and dikaryotic development, respectively. The sites were strongly enriched near translation initiation sites of genes. Although the overall epigenetic landscape was similar between both conditions, we identified 837 sites of differential enrichment during monokaryotic or dikaryotic development, associated with 965 genes. Six transcription factor genes were enriched in H3K4me2 during dikaryotic development, indicating that these are epigenetically regulated during development. Deletion of two of these genes (fst1 and zfc7) resulted in arrested development of fruiting bodies, resulting in immature mushrooms. Together these results indicate that H3K4me2 ChIP-Seq is a powerful new tool to map the restructuring of the epigenetic landscape during mushroom development. Moreover, it can be used to identify novel developmental regulators.


Assuntos
Sequenciamento de Cromatina por Imunoprecipitação/métodos , Histonas/metabolismo , Schizophyllum/crescimento & desenvolvimento , Fatores de Transcrição/genética , Epigênese Genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Deleção de Genes , Metilação , Iniciação Traducional da Cadeia Peptídica , Schizophyllum/genética
4.
PLoS One ; 16(1): e0245623, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33449959

RESUMO

Wood is a habitat for a variety of organisms, including saprophytic fungi and bacteria, playing an important role in wood decomposition. Wood inhabiting fungi release a diversity of volatiles used as signaling compounds to attract or repel other organisms. Here, we show that volatiles of Schizophyllum commune are active against wood-decay fungi and bacteria found in its mycosphere. We identified sesquiterpenes as the biologically active compounds, that inhibit fungal growth and modify bacterial motility. The low number of cultivable wood inhabiting bacteria prompted us to analyze the microbial community in the mycosphere of S. commune using a culture-independent approach. Most bacteria belong to Actinobacteria and Proteobacteria, including Pseudomonadaceae, Sphingomonadaceae, Erwiniaceae, Yersiniaceae and Mariprofundacea as the dominating families. In the fungal community, the phyla of ascomycetes and basidiomycetes were well represented. We propose that fungal volatiles might have an important function in the wood mycosphere and could meditate interactions between microorganisms across domains and within the fungal kingdom.


Assuntos
Actinobacteria/metabolismo , Proteobactérias/metabolismo , Schizophyllum/metabolismo , Sesquiterpenos/metabolismo , Microbiologia do Solo
5.
J Infect Chemother ; 27(5): 759-765, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33388231

RESUMO

This is a case report of allergic fungal rhinosinusitis caused by Schizophyllum commune (S. commune) identified in a patient's nasal mucus and environmental soil sample using (r)DNA sequencing. Although filamentous basidiomycetes, including S. commune, are known as environmental pathogens causing allergic respiratory diseases worldwide, many patients with infections caused by S. commune have not been correctly diagnosed. Repeated exposures to environmental floating fungi supposedly make an easy sensitization and colonization of fungi in the nasal passages, resulting in the onset of allergic fungal rhinosinusitis due to S. commune in our living environments. This report indicates the importance of reconsidering allergic respiratory diseases associated with our living environments.


Assuntos
Schizophyllum , Humanos , Muco , Schizophyllum/genética , Análise de Sequência de DNA , Solo , Escarro
6.
J Biosci Bioeng ; 131(4): 348-355, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33281068

RESUMO

The chitin-assimilating gram-negative bacterium, Lysobacter sp. MK9-1, was isolated from soil and was the source of a glycoside hydrolase family 19-type chitinase (Chi19MK) gene that is 933-bp long and encodes a 311-residue protein. The deduced amino acid sequence of Chi19MK includes a signal peptide, an uncharacterized sequence, a carbohydrate-binding module family 12-type chitin binding domain, and a catalytic domain. The catalytic domain of Chi19MK is approximately 60% similar to those of ChiB from Burkholderia gladioli CHB101, chitinase N (ChiN) from Chitiniphilus shinanonensis SAY3T, ChiF from Streptomyces coelicolor A3(2), Chi30 from Streptomyces olivaceoviridisis, ChiA from Streptomyces cyaneus SP-27, and ChiC from Streptomyces griseus HUT6037. Chi19MK lacking the signal and uncharacterized sequences (Chi19MKΔNTerm) was expressed in Escherichia coli Rosetta-gami B(DE3), resulting in significant chitinase activity in the soluble fraction. Purified Chi19MKΔNTerm hydrolyzed colloidal chitin and released disaccharide. Furthermore, Chi19MKΔNTerm inhibited hyphal extension in Trichoderma reesei and Schizophyllum commune. Based on quantitative antifungal activity assays, Chi19MKΔNTerm inhibits the growth of Trichoderma viride with an IC50 value of 0.81 µM.


Assuntos
Antifúngicos/farmacologia , Quitinases/metabolismo , Lysobacter/enzimologia , Quitinases/genética , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Lysobacter/genética , Schizophyllum/efeitos dos fármacos , Trichoderma/efeitos dos fármacos
7.
J Hazard Mater ; 403: 124002, 2021 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-33265035

RESUMO

Radioactive contamination resulting from major nuclear accidents presents harsh environmental conditions. Inside the Chernobyl exclusion zone, even more than 30 years after the accident, the resulting contamination levels still does not allow land-use or human dwellings. To study the potential of basidiomycete fungi to survive the conditions, a field trial was set up 5 km south-south-west of the destroyed reactor unit. A model basidiomycete, the lignicolous fungus Schizophyllum commune, was inoculated and survival in the soil could be verified. Indeed, one year after inoculation, the fungus was still observed using DNA-dependent techniques. Growth led to spread at a high rate, with approximately 8 mm per day. This shows that also white-rot basidiomycetes can survive the harsh conditions in soil inside the Chernobyl exclusion zone. The unadapted fungal strain showed the ability to grow and thrive in the contaminated soil where both stress from radiation and heavy metals were present.


Assuntos
Acidente Nuclear de Chernobyl , Metais Pesados , Schizophyllum , Solo
8.
Carbohydr Polym ; 253: 117285, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33278951

RESUMO

ß-glucans are potent immunomodulators, with effects on innate and adaptive immune responses via dectin-1 as the main receptor. In this study, we investigated the biological effect of ß-glucan from Schizophyllum commune, called Schizophyllan (SPG) on Interleukin-10 (IL-10) expression induced by a lipopolysaccharide (LPS) from Aggregatibacter actinomycetemcomitans in murine macrophages (J774.1). SPG and dectin-1 interaction up-regulates LPS-induced IL-10 expression. The regulative effect of SPG on IL-10 expression is dependent on prolongation of nuclear translocation activity of nuclear factor-kappa B (NF-κBα) pathway induced by LPS. We also found that LPS-induced phosphorylation of mitogen- and stress-activated protein kinase 1 (MSK1) and cAMP-responsive-element-binding protein (CREB), followed by up-regulation of IL-10, was stimulated by SPG priming via activation of the spleen tyrosine kinase (Syk). Our data indicate that SPG augments the anti-inflammatory response in murine macrophages which can be useful to create an intervention for periodontal disease treatment.


Assuntos
Adjuvantes Imunológicos/farmacologia , Aggregatibacter actinomycetemcomitans/química , Polissacarídeos Fúngicos/farmacologia , Interleucina-10/metabolismo , Lipopolissacarídeos/farmacologia , Macrófagos/imunologia , Schizophyllum/química , Sizofirano/farmacologia , Adjuvantes Imunológicos/metabolismo , Animais , Polissacarídeos Fúngicos/metabolismo , Lectinas Tipo C/metabolismo , Macrófagos/efeitos dos fármacos , Camundongos , NF-kappa B/metabolismo , Infecções por Pasteurellaceae/tratamento farmacológico , Infecções por Pasteurellaceae/microbiologia , Doenças Periodontais/tratamento farmacológico , Doenças Periodontais/microbiologia , Fosforilação/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Sizofirano/metabolismo
9.
Bioprocess Biosyst Eng ; 44(2): 317-328, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32955618

RESUMO

Schizophyllum commune is a wood-rotting filamentous fungus that secrets a homopolysaccharide called as schizophyllan. Schizophyllan has several applications such as enhanced oil recovery, pharmaceutical materials and an anti-cancer drug carrier. Biomass growth and schizophyllan production increase the viscosity of the cultivation medium, thus resulting in mass transfer limitation for the substrate. In this study, adding talc and aluminium oxide microparticles into the cultivation medium was studied to improve the fungal growth and morphology. The response surface methodology and one factor at a time were applied to find the effects of microparticles with different sizes and concentrations on the schizophyllan production. The optimum concentration and size of aluminium oxide microparticles were obtained as 20 g L-1 and < 30 µm, respectively. Aluminium oxide microparticles in shake flask culture caused to increase the schizophyllan production from 10 to 15 g L-1 and decrease the cultivation time from 10 to 7 days. The production yield also increased from 0.11 to 0.30 g of schizophyllan/g glucose. Bioreactor cultivation showed a twofold increase in schizophyllan production from 1.5 to 3 g L-1. The results of this study suggested a significant increase in the production of schizophyllan using a low-cost "microparticle-enhanced cultivation" without any further optimization of the culture medium.


Assuntos
Biomassa , Reatores Biológicos , Meios de Cultura , Schizophyllum/crescimento & desenvolvimento , Sizofirano/biossíntese , Óxido de Alumínio/química , Talco/química
10.
Environ Microbiol ; 23(2): 1174-1185, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33215844

RESUMO

Fungi dominated the eukaryotic group in the anaerobic sedimentary environment below the ocean floor where they play an essential ecological role. However, the adaptive mechanism of fungi to these anaerobic environments is still unclear. Here, we reported the anaerobic adaptive mechanism of Schizophyllum commune 20R-7-F01, isolated from deep coal-bearing sediment down to ~2 km below the seafloor, through biochemical, metabolomic and transcriptome analyses. The fungus grows well, but the morphology changes obviously and the fruit body develops incompletely under complete hypoxia. Compared with aerobic conditions, the fungus has enhanced branched-chain amino acid biosynthesis and ethanol fermentation under anaerobic conditions, and genes related to these metabolisms have been significantly up-regulated. Additionally, the fungus shows novel strategies for synthesizing ethanol by utilizing both glycolysis and ethanol fermentation pathways. These findings suggest that the subseafloor fungi may adopt multiple mechanisms to cope with lack of oxygen.


Assuntos
Sedimentos Geológicos/microbiologia , Schizophyllum/isolamento & purificação , Schizophyllum/fisiologia , Água do Mar/microbiologia , Aminoácidos de Cadeia Ramificada/biossíntese , Anaerobiose , Carvão Mineral/análise , Etanol/metabolismo , Fermentação , Regulação Fúngica da Expressão Gênica , Genes Fúngicos/genética , Sedimentos Geológicos/química , Schizophyllum/genética , Schizophyllum/metabolismo , Água do Mar/química
11.
Infection ; 49(4): 775-779, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33156492

RESUMO

BACKGROUND: The pathogenic fungus Schizophyllum sp. can cause allergic fungal rhinosinusitis and allergic bronchopulmonary mycosis in humans. Sinus and lung infections due to Schizophyllum sp. have been reported globally; however, no case of hypertrophic pachymeningitis due to this pathogen has been reported yet. Herein, we describe for the first time, a case of hypertrophic pachymeningitis due to Schizophyllum sp. CASE PRESENTATION: A 69-year-old woman visited the hospital with chief complaints of headache, right trigeminal neuralgia (third branch), ataxic gait, and deafness in the right ear. Magnetic resonance imaging revealed a tumor in the right sphenoidal sinus and thickening of the dura mater surrounding the right porus acusticus internus. Endoscopic sinus surgery and neuroendoscopic biopsy were performed to remove sinus lesions and intracranial lesions, respectively. Both pathological specimens showed findings indicative of filamentous fungi on Grocott's staining. DNA sequencing with the sinus specimen revealed Schizophyllum sp. as the causative pathogen, consistent with the diagnosis of fungal sinusitis and hypertrophic pachymeningitis. Intravenous liposomal amphotericin B was started, but owing to lack of improvement, the treatment was switched to intravenous voriconazole. We observed improvements in both radiological findings and symptoms. However, the symptoms exacerbated again when the trough level of voriconazole decreased. Upon increasing the voriconazole dose, a higher trough level was obtained and the symptoms improved. CONCLUSION: Our study suggests that when symptoms of central nervous system infection due to Schizophyllum sp. do not improve with liposomal amphotericin B, voriconazole can be administered at high trough levels to improve the symptoms.


Assuntos
Meningite , Micoses , Schizophyllum , Sinusite , Idoso , Feminino , Humanos , Imageamento por Ressonância Magnética , Meningite/diagnóstico , Meningite/tratamento farmacológico , Sinusite/diagnóstico , Sinusite/tratamento farmacológico , Voriconazol
12.
Gen Thorac Cardiovasc Surg ; 69(3): 584-587, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33118109

RESUMO

Schizophyllum commune (S. commune) is an environmental basidiomycete bracket fungus that can rarely cause invasive fungal disease. Its diagnosis is challenging and often missed. We present a rare case of a 56-year-old diabetic gentleman with a 6-months history of progressive productive cough, small volume hemoptysis and non-resolving fungal empyema treated with video-assisted thoracoscopic decortication. Pulmonary infection of S. Commune is extremely rare and can manifest as complex fungal empyema. Close cooperation between respirologists, thoracic surgeons and microbiologists is critical in the diagnosis and treatment of such cases.


Assuntos
Empiema Pleural , Schizophyllum , Cirurgia Torácica Vídeoassistida , Empiema Pleural/diagnóstico por imagem , Empiema Pleural/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade
13.
PLoS One ; 15(12): e0244290, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33347481

RESUMO

Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol (4-VG), the impact compound of smoke flavour. The first basidiomycetous ferulic acid decarboxylase (FAD) was isolated from Schizophyllum commune (ScoFAD) and heterologously produced by Komagataella phaffii. It showed a molecular mass of 21 kDa, catalytic optima at pH 5.5 and 35°C, and a sequence identity of 63.6% to its next relative, a FAD from the ascomycete Cordyceps farinosa. The ScoFAD exhibited a high affinity to its only known substrate ferulic acid (FA) of 0.16 mmol L-1 and a turnover number of 750 s-1. The resulting catalytic efficiency kcat KM-1 of 4,779 L s-1 mmol-1 exceeded the next best known enzyme by more than a factor of 50. Immobilised on AminoLink Plus Agarose, ScoFAD maintained its activity for several days. The combination with FAEs and agro-industrial side-streams paves the way for a new generation of sustainable, clean, and safe smoke flavours.


Assuntos
Aromatizantes/síntese química , Guaiacol/análogos & derivados , Carboxiliases/química , Carboxiliases/isolamento & purificação , Carboxiliases/metabolismo , Hidrolases de Éster Carboxílico , Carcinógenos , Cordyceps/metabolismo , Ácidos Cumáricos/química , Aromatizantes/química , Guaiacol/síntese química , Guaiacol/química , Saccharomycetales/metabolismo , Schizophyllum/metabolismo
14.
Int J Med Mushrooms ; 22(7): 659-669, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32865923

RESUMO

The present study aimed to evaluate the antibacterial activity (ABA) of new mushroom strains collected from the mountain and plain forests of Georgia and belonging to different taxonomic groups. Of 30 Basidiomycetes strains tested on agar plates, Schizophyllum commune BCC64 exhibited the highest inhibitory activity against Escherichia coli and Staphylococcus aureus by the diameter of inhibition zones (17 ± 1 mm and 19 ± 1 mm, respectively). Moreover, this mushroom showed strong activity against Staphylococcus enteritidis (11 mm), Pseudomonas aeruginosa (19 mm), and Salmonella epidermitidis (12 mm). In the submerged cultivation in synthetic medium, xylose and glucose ensured the highest ABA toward S. aureus (70% inhibition in microplate rider tests) and E. coli (60%), respectively. Among lignocellulosic materials tested in the submerged and solid-state fermentation, mandarin marc was found to be an excellent growth substrate for ABA accumulation by Sch. commune 64. Of six nitrogen sources, KNO3 favored the mushroom ABA increase against both bacteria. The suitability of the developed nutrient medium has been proven in 7 L fermenter. After fermentation, ethyl acetate extract obtained from culture liquid and ethanol extract obtained from mycelial biomass of Sch. commune 64 showed the best minimum inhibitory concentration (MIC) against E. coli (0.5 and 2.5 mg/mL, respectively) and S. aureus (1 mg/mL for both extracts).


Assuntos
Antibacterianos/isolamento & purificação , Schizophyllum/química , Schizophyllum/crescimento & desenvolvimento , Antibacterianos/farmacologia , Avaliação Pré-Clínica de Medicamentos , Escherichia coli/efeitos dos fármacos , República da Geórgia , Nitrogênio/metabolismo , Staphylococcus aureus/efeitos dos fármacos
15.
J Microbiol Biotechnol ; 30(10): 1525-1535, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-32807761

RESUMO

Synthetic dyes are widely used in various industries and their wastage causes severe environmental problems while being hazardous to human health, leading to the need for eco-friendly degradation techniques. The split-gill fungus Schizophyllum commune, which is found worldwide, has the potential to degrade all components of the lignocellulosic biomass and is a candidate for the treatment of synthetic dyes. A systematic molecular analysis of 75 Korean and 6 foreign S. commune strains has revealed the high genetic diversity of this population and its important contribution to the total diversity of S. commune. We examined the dye decolorization ability of this population and revealed 5 excellent strains that strongly decolorized 3 dyes: Crystal Violet, Congo Red and Methylene Blue. Finally, comparison of dye decolorization ability and the phylogenetic identification of these strains generalized their genetic and physiological diversity. This study provides an initial resource for physiological and genetic research projects as well as the bioremediation of textile dyes.


Assuntos
Biodegradação Ambiental , Corantes/metabolismo , Variação Genética , Schizophyllum/genética , Schizophyllum/metabolismo , Biomassa , Vermelho Congo/metabolismo , DNA Fúngico/genética , DNA Fúngico/isolamento & purificação , Violeta Genciana/metabolismo , Azul de Metileno/metabolismo , Filogenia , República da Coreia , Schizophyllum/classificação , Análise de Sequência de DNA , Têxteis/microbiologia , Purificação da Água
16.
Commun Biol ; 3(1): 334, 2020 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-32591629

RESUMO

Fungal mycelium is an emerging bio-based material. Here, mycelium films are produced from liquid shaken cultures that have a Young's modulus of 0.47 GPa, an ultimate tensile strength of 5.0 MPa and a strain at failure of 1.5%. Treating the mycelial films with 0-32% glycerol impacts the material properties. The largest effect is observed after treatment with 32% glycerol decreasing the Young's modulus and the ultimate tensile strength to 0.003 GPa and 1.8 MPa, respectively, whereas strain at failure increases to 29.6%. Moreover, glycerol treatment makes the surface of mycelium films hydrophilic and the hyphal matrix absorbing less water. Results show that mycelium films treated with 8% and 16-32% glycerol classify as polymer- and elastomer-like materials, respectively, while non-treated films and films treated with 1-4% glycerol classify as natural material. Thus, mycelium materials can cover a diversity of material families.


Assuntos
Glicerol/farmacologia , Micélio/classificação , Materiais Biocompatíveis , Biofilmes/classificação , Biofilmes/efeitos dos fármacos , Biomassa , Microscopia , Microscopia Eletrônica de Varredura , Micélio/efeitos dos fármacos , Micélio/fisiologia , Micélio/ultraestrutura , Schizophyllum/efeitos dos fármacos , Schizophyllum/crescimento & desenvolvimento , Resistência à Tração/efeitos dos fármacos , Água/metabolismo
17.
Food Chem ; 329: 127089, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32516705

RESUMO

Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC). The content of phenolics and antioxidant activity were determined for the substrates and mushrooms produced. The content of ß-glucans and the composition of S. commune were also evaluated. The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity. Mushrooms grown in this condition showed the highest value (13.14%) of ß-glucans. The contents of proteins and dietary fibre were 16.59% and 59.61%, respectively. Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging). The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.


Assuntos
Antioxidantes/química , Biocombustíveis , Schizophyllum/metabolismo , Fibras na Dieta , Glucanos/metabolismo , Fenóis/metabolismo , Schizophyllum/química , Vitis/metabolismo
18.
Rev. iberoam. micol ; 37(2): 65-67, abr.-jun. 2020.
Artigo em Espanhol | IBECS | ID: ibc-199134

RESUMO

ANTECEDENTES: Schizophyllum commune es un hongo basidiomiceto ampliamente distribuido en la naturaleza. Su papel como responsable de enfermedad en el ser humano ha sido poco conocido, en parte debido a su difícil identificación. La incorporación a los laboratorios de técnicas de espectrometría de masas (MALDI-TOF) y biología molecular ha permitido la descripción de un mayor número de casos. CASO CLÍNICO: En este trabajo presentamos dos casos en los que se identificó S. commune como agente causal de enfermedad: un caso de rinosinusitis crónica en un paciente inmunocompetente y otro caso de infección del seno esfenoidal en un paciente inmunocomprometido. En ambos casos se aisló S. commune. Su identificación fue posible gracias al MALDI-TOF y esta se confirmó en ambos pacientes mediante la amplificación y secuenciación de la región ITS. CONCLUSIONES: Concluimos que S. commune debe ser considerado un posible agente causal de enfermedad micótica. Actualmente, las técnicas de MALDI-TOF y secuenciación son necesarias para su identificación


BACKGROUND: Schizophyllum commune is a basidiomycete fungus which is widely distributed in nature. Its role as responsible for disease in humans is not well known, partly due to its difficult identification. The incorporation of mass spectrometry techniques (MALDI-TOF) and molecular biology to the laboratories has allowed the description of a greater number of cases. CASE REPORT: In this paper, we present two cases in which S. commune was identified as the causative agent of disease: in the first case an immunocompetent patient suffered from chronic rhinosinusitis, and in the second one a sphenoid sinus infection was diagnosed in an immunocompromised patient. In both cases, S. commune was isolated. Its identification was possible by means of MALDI-TOF and this was confirmed in both patients by amplification and sequencing of the ITS region. CONCLUSIONS: In conclusion, S. commune should be considered a potential causative agent of fungal disease. Currently, MALDI-TOF and sequencing techniques are necessary for its identification


Assuntos
Humanos , Masculino , Adulto , Idoso , Hospedeiro Imunocomprometido , Sinusite/microbiologia , Rinite/microbiologia , Schizophyllum/isolamento & purificação , Micoses/microbiologia
19.
Rev Iberoam Micol ; 37(2): 65-67, 2020.
Artigo em Espanhol | MEDLINE | ID: mdl-32376274

RESUMO

BACKGROUND: Schizophyllum commune is a basidiomycete fungus which is widely distributed in nature. Its role as responsible for disease in humans is not well known, partly due to its difficult identification. The incorporation of mass spectrometry techniques (MALDI-TOF) and molecular biology to the laboratories has allowed the description of a greater number of cases. CASE REPORT: In this paper, we present two cases in which S. commune was identified as the causative agent of disease: in the first case an immunocompetent patient suffered from chronic rhinosinusitis, and in the second one a sphenoid sinus infection was diagnosed in an immunocompromised patient. In both cases, S. commune was isolated. Its identification was possible by means of MALDI-TOF and this was confirmed in both patients by amplification and sequencing of the ITS region. CONCLUSIONS: In conclusion, S. commune should be considered a potential causative agent of fungal disease. Currently, MALDI-TOF and sequencing techniques are necessary for its identification.


Assuntos
Sinusite Maxilar/microbiologia , Micoses/microbiologia , Schizophyllum/isolamento & purificação , Sinusite Esfenoidal/microbiologia , Adulto , Idoso , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Farmacorresistência Fúngica , Corpos Estranhos/complicações , Humanos , Masculino , Mucocele/complicações , Schizophyllum/efeitos dos fármacos , Schizophyllum/patogenicidade
20.
BMC Microbiol ; 20(1): 139, 2020 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-32471364

RESUMO

BACKGROUND: In the last few decades, considerable attention has been paid to fungal endophytes as biocontrol agents, however little is known about their mode of action. This study aimed to investigate the toxic effects of an endophytic fungus Schizophyllum commune by analyzing activities of antioxidant and detoxifying enzymes as well as morphology of haemocytes using Spodoptera litura as a model. RESULTS: Ethyl acetate extract of S. commune was fed to the larvae of S. litura using the artificial diet having 276.54 µg/ml (LC50 of fungus) concentration for different time durations. Exposed groups revealed significant (p ≤ 0.05) increase in the activities of various enzymes viz. Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase. Furthermore, haemocytes showed various deformities like breakage in the cell membrane, cytoplasmic leakage and appearance of strumae in the treated larvae. A drastic reduction in the percentage of normal haemocytes was recorded in the treated groups with respect to control. CONCLUSION: The study provides important information regarding the oxidative stress causing and immunosuppressant potential of S. commune against S. litura and its considerable potential for incorporation in pest management programs.


Assuntos
Produtos Biológicos/farmacologia , Imunossupressores/farmacologia , Schizophyllum/patogenicidade , Spodoptera/microbiologia , Animais , Produtos Biológicos/isolamento & purificação , Enzimas/genética , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Hemócitos/efeitos dos fármacos , Imunossupressores/isolamento & purificação , Proteínas de Insetos/genética , Estresse Oxidativo , Controle de Pragas , Schizophyllum/química , Spodoptera/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...