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1.
J Environ Manage ; 301: 113837, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34592668

RESUMO

The hyperaccumulating mechanism concerning heavy metal activation or passivation and plant response triggered by fulvic acid (FA) and humic acid (HA) recruitments are investigated herein. We carefully examine the Cd activation effect by various FA and HA, tracing from pig, goat, and duck manure composts to straw compost and commercial materials (i.e., PC, GC, DC, SC, and CM), as well as their roles in plant growth promotion and Cd uptake. Our results indicate that due to the decrease of soil pH and their multiple functional groups, the contents of available Cd (AE-Cd) increased by 4.3-4.8% and 3.6-6.3% when all FA and HA sources were applied for 30 days. A 13.1-19.9% increase in AE-Cd was observed when CFA, DFA, and PFA were applied for five days, and a 9.5% increment was found when PHA was applied for 10 days. In the pot experiment, the Cd accumulation in plants increased by 2.78 and 2.17 folds with PFA and PHA applications, respectively, compared to the blank control group. This result can be attributed to the stimulative effects of the simultaneous Sedum alfredii growth and Cd phytoavailability. Notably, the Cd accumulation increased by 2.26 times with the SFA amendment due to the predominant stimulation effect to the phytoavailable Cd rather than plant growth. However, slight inhibitory effects were observed upon plant growth or Cd uptake, which led to the reduction of the Cd accumulation with DHA, SHA, and CHA employments. Consistently, the corresponding soil Cd removal efficiencies were 43.5% and 34.6% with PFA and PHA, respectively, which hold abundant O- and N-containing groups. Our research aims to gain insights into the ternary interaction in the presence of heavy metal, humic substances, and S. alfredii to simultaneously accelerate Cd activation and hyperaccumulation.


Assuntos
Compostagem , Sedum , Poluentes do Solo , Animais , Biodegradação Ambiental , Cádmio/análise , Substâncias Húmicas , Raízes de Plantas/química , Solo , Poluentes do Solo/análise , Suínos
2.
PLoS One ; 16(9): e0257172, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34492083

RESUMO

As an essential element, zinc (Zn) can improve or inhibit the growth of plants depending on its concentrations. In this study, the effects of 24-Epibrassinolide (EBR), one well-known steroid phytohormone regulating plant growth and alleviating abiotic stress damage, on morphological parameters and antioxidant capacities of Sedum lineare were investigated under different Zn doses. Compared to plants only exposed to Zn, simultaneously foliar application of 0.75 µM EBR significantly improved multiple morphological characteristics and such growth-improving effects were more significant at high Zn concentrations. At a detrimental 800 µM Zn, EBR benefitted plant growth most prominently, as shown by that the stem length, fresh weight and internode length were increased by 111%, 85% and 157%, respectively; than Zn solely treated plants. EBR spray also enhanced both the activities of antioxidant enzymes such as peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR), and the contents of antioxidative agents including ascorbic acid (AsA) and glutathione (GSH), which in turn decreased the accumulation of reactive oxygen species (ROS) and alleviated the lipid peroxidation in plants. Thus, by demonstrating that EBR could help S. lineare resist high-zinc stress through strengthening the antioxidant system, this work provided a new idea for expanding the planting range of Crassulaceae plants in heavy metal contaminated soil for phytoremediation purpose in the future.


Assuntos
Antioxidantes/farmacologia , Brassinosteroides/farmacologia , Sedum/crescimento & desenvolvimento , Esteroides Heterocíclicos/farmacologia , Estresse Fisiológico , Zinco/toxicidade , Glutationa/metabolismo , Peróxido de Hidrogênio/metabolismo , Malondialdeído/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/enzimologia , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/enzimologia , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento , Sedum/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Superóxidos/metabolismo
3.
Molecules ; 26(15)2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34361655

RESUMO

Pterygium is a corneal alteration that can cause visual impairment, which has been traditionally treated with the sap of Sedum dendroideum D.C. The pharmacological effect of a dichloromethane extract of S. dendroideum was demonstrated and implemented in a pterygium model on the healing process of corneal damage caused by phorbol esters. In mice of the ICR strain, a corneal lesion was caused by intravitreal injection of tetradecanoylphorbol acetate (TPA). The evolution of the corneal scarring process was monitored with vehicle, dexamethasone, and dichloromethane extract of S. dendroideum treatments by daily ophthalmic administration for fifteen days. The lesions were evaluated in situ with highlighted images of fluorescence of the lesions. Following treatment levels in eyeballs of IL-1α, TNF-α, and IL-10 cytokines were measured. The effective dose of TPA to produce a pterygium-like lesion was determined. The follow-up of the evolution of the scarring process allowed us to define that the treatment with S. dendroideum improved the experimental pterygium and had an immunomodulatory effect by decreasing TNF-α, IL-1α, and maintaining the level of IL-10 expression, without difference with respect to the healthy control. Traditional medical use of S. dendroideum sap to treat pterygium is fully justified by its compound composition.


Assuntos
Anti-Inflamatórios/farmacologia , Túnica Conjuntiva/anormalidades , Cloreto de Metileno/farmacologia , Extratos Vegetais/farmacologia , Pterígio/tratamento farmacológico , Cicatrização/efeitos dos fármacos , Animais , Masculino , Camundongos , Camundongos Endogâmicos ICR , Sedum/química
4.
Phytomedicine ; 91: 153670, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34391081

RESUMO

BACKGROUND: Sedum species are reported to possess diverse pharmacological activities in various solid tumors. However, the anticancer functions of Sedum orizyfolium and its constituents have never been determined in human cancers. PURPOSE: The present study focused on addressing the inhibition efficacy of the methanol extract of S. orizyfolium (MESO) and its constituents and the molecular mechanism underlying invasion and epithelial-to-mesenchymal transition (EMT) in oral squamous cell carcinoma (OSCC) cell lines. STUDY DESIGN/METHODS: After MESO treatment, a wound-healing assay, an invasion assay, and immunocytochemistry were performed in OSCC cell lines, coupled with in silico analysis and immunohistochemistry in OSCC patient samples, to investigate the role of the EMT transcription factor Slug. Trehalose, an active component of MESO, was identified through gas chromatography-mass spectrometry. RESULTS: Among the methanol extracts of 18 various wild plants from South Korea, MESO exhibited the highest anticancer functionality in OSCC cells by downregulating Slug expression. In silico analysis and immunohistochemistry indicated that elevated Slug levels are remarkably associated with tumor progression and invasion in patients with OSCC, suggesting that changes in Slug expression alter EMT progression and invasion in OSCC. Notably, treatment with trehalose, a sugar component of MESO, inhibited invasiveness and Slug expression in OSCC cells. CONCLUSION: Cumulatively, this study highlighted the beneficial role of MESO and trehalose in the inhibition of invasiveness of OSCC cells via suppression of Slug expression and suggested a new design for potential chemotherapeutic drugs against OSCC.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Extratos Vegetais , Sedum , Fatores de Transcrição da Família Snail/metabolismo , Trealose/farmacologia , Carcinoma de Células Escamosas/tratamento farmacológico , Linhagem Celular Tumoral , Movimento Celular , Regulação para Baixo , Transição Epitelial-Mesenquimal , Regulação Neoplásica da Expressão Gênica , Humanos , Metanol , Neoplasias Bucais/tratamento farmacológico , Invasividade Neoplásica , Extratos Vegetais/farmacologia , Sedum/química , Carcinoma de Células Escamosas de Cabeça e Pescoço
5.
Environ Pollut ; 285: 117504, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34380216

RESUMO

Soil trace elements (TEs) contamination has become a worldwide problem in arable lands and poses great risk to human health via food chain. Intercropping of hyperaccumulator and cash crops is now proposed as a promising alternative phytoremediation technique to address the issue. However, the effect of intercropping in different soil types and field-scale benefits evaluation are rarely reported. A greenhouse pot experiment and a field trial were therefore designed to explore the effects of intercropping Sedum alfredii (hyperaccumulative population) and oilseed rape on Cd phytoextraction potential, Cd transport and crop production, as well as establishing a feasible assessment framework on the basis of benefits evaluation in contaminated soils. Compared with oilseed rape monoculture, intercropping with S. alfredii significantly and consistently increased biomass, seed yield and Cd accumulation in oilseed rape in five typical soil types. Accumulations of Cd varied with soil types, ranging from 22.8 to 4000 µg pot-1. Stepwise multiple linear regression analysis (SMLRA) showed Cd concentrations in plants were related to available phosphorus (AP), pH, soil organic matter (OM), available potassium (AK), silt and sand; R2 values varied from 0.834 to 0.994 (P < 0.05). A field trial also verified that intercropping could significantly enhance Cd phytoextraction. The highest index for comprehensive benefits evaluation was 0.61 observed in the S. alfredii and oilseed rape intercropping system. This system presented higher Cd phytoextraction potential and comprehensive benefits index whilst allowing ongoing agricultural activities in slightly and moderately Cd-contaminated soils. These results provide a possible technical approach for phytoremediation practice and give new insights into theoretical reference for development of Cd phytoextraction and benefits evaluation.


Assuntos
Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Humanos , Solo , Poluentes do Solo/análise
6.
J Hazard Mater ; 419: 126489, 2021 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-34216961

RESUMO

Phytoremediation is a green technology for heavy metal removal from contaminated soil, and its remediation efficiency and economic feasibility in field trial should be evaluated before large-scale application. However, there is still lacking relevant analysis, especially for phytoremediation with different cropping patterns. In the present study, we performed phytoremediation on slightly Cd-contaminated farmland soil via three cropping systems, i.e. Sedum alfredii monoculture, oilseed rape monoculture, and S. alfredii-oilseed rape intercropping. Dry weights of S. alfredii and oilseed rape were both enhanced under intercropping pattern, while the highest total Cd extraction amount (148 g ha-1) were observed under S. alfredii monoculture. Furtherly, a cost-benefit analysis via Monta Carlo simulation in a ten-year lifetime was conducted. The benefits of S. alfredii monoculture and intercropping schemes would offset the total costs in 6 and 8 years, respectively. S. alfredii monoculture achieved a higher net present value of 1.88 × 104 US$ as compared with intercropping (9.53 × 103 US$). These results indicate that S. alfredii monoculture scheme could be a promising phytoremediation strategy for slightly Cd-contaminated soil owing to better remediation efficiency and economic feasibility. Moreover, the enhancement in mechanization level and the reduction of seedling cost could further improve its economic viability.


Assuntos
Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Análise Custo-Benefício , Fazendas , Solo , Poluentes do Solo/análise
7.
Chemosphere ; 285: 131420, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34256202

RESUMO

Exogenous application of plant-growth promoting substances in combination with chelators is a common way to enhance the phytoextraction of heavy metals. A pot experiment was used to explore the influences of indole-3-acetic acid (IAA)/gibberellin (GA3) alone or together with oxalic acid (OA) on the growth, physiological response, and nutrient contents of hyperaccumulator Sedum alfredii Hance, and cadmium (Cd) and lead (Pb) phytoextraction efficiency. The results showed that a foliar spray of IAA/GA3 alone or together with OA increased plant growth. The largest shoot biomass with increase by 29.7% was produced by the 50 µmol L-1 IAA combined with 2.5 mmol kg-1 OA (50I+2.5OA) treatment as compared with the control treatment (CK). The presence of IAA and GA3 enhanced the chlorophyll a, carotenoid, and potassium contents in leaves and decreased the malondialdehyde content. The Cd content in leaf and the translocation factor (TFshoot) value from 50I+2.5OA treatment was increased by 4.29% and 21.4%, and the Pb content in stem and shoot, and the TFshoot of Pb after applying 50 µmol L-1 GA3 combined with 2.5 mmol kg-1 OA was enhanced by 32.5%, 13.4%, and 57.6%, compared with CK, respectively. The optimal Cd and Pb phytoextraction efficiency occurred from 50I+2.5OA treatment with increase by 82.4% and 79.3% as compared with CK, respectively. Therefore, the results showed that a combined application of 50 µmol L-1 IAA and 2.5 mmol kg-1 OA could effectively enhance S. alfredii Hance phytoremediation of Cd and Pb co-contaminated soil.


Assuntos
Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Clorofila A , Giberelinas , Ácidos Indolacéticos , Chumbo , Ácido Oxálico , Raízes de Plantas/química , Solo , Poluentes do Solo/análise
8.
Fish Physiol Biochem ; 47(4): 1149-1164, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34142329

RESUMO

Diet-induced fatty liver is a considerable threaten to fish aquaculture due to the popularity of the high-fat diet (HFD) feeding. Our study aims to investigate the effects of flavanones from Sedum sarmentosum Bunge (FSSB) on the liver function to identify a potential treatment for HFD-induced fatty liver disease. Physiological and pathological indicators were tested in the liver of Nile tilapia (Oreochromis niloticus) and results showed parameters including lipid metabolites, redox parameters, and inflammatory factors could be adequately restored to normal level by addition of 150 mg/kg FSSB to HFD. Proteomics analysis was performed in liver tissues from tilapia with normal diet (ND), HFD, and HFD+FSSB. Totally, 51 upregulated proteins and 77 downregulated proteins were identified in HFD groups and 67 proteins of them were restored after treated with FSSB. Bioinformatics analysis showed that differentially expressed proteins (DEPs) in HFD+FSSB150 group compared with HFD group are mainly enriched in acety-CoA metabolic process, adenosine-triphosphate (ATP) biosynthetic process, lipid metabolic process, and phospholipid metabolic process. The dysregulated proteins were involved in peroxidosome proliferators-activated receptor (PPAR) signaling pathway, fat digestion and absorption, and immune system. The quantitative real-time PCR (qRT-PCR) assay further revealed that the expression of GST, PPARα, PPARγ, and multiple-inflammatory cytokines could be also reversed in HFD group under the treatment of 150 mg/kg FSSB. Our findings demonstrated FSSB is efficient for the treatment of fatty liver disease through regulation of lipid metabolism and antioxidation in Nile tilapia, providing a new treatment of non-alcoholic fatty liver disease (NAFLD) in fish aquaculture.


Assuntos
Antioxidantes/uso terapêutico , Ciclídeos , Fígado Gorduroso/tratamento farmacológico , Doenças dos Peixes/tratamento farmacológico , Flavanonas/uso terapêutico , Sedum , Animais , Antioxidantes/farmacologia , Colesterol/sangue , Ciclídeos/sangue , Ciclídeos/genética , Ciclídeos/crescimento & desenvolvimento , Dieta Hiperlipídica , Fígado Gorduroso/genética , Fígado Gorduroso/veterinária , Doenças dos Peixes/genética , Proteínas de Peixes/genética , Flavanonas/farmacologia , Glutationa Transferase/genética , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , PPAR alfa/genética , PPAR gama/genética , Triglicerídeos/sangue
9.
Chemosphere ; 276: 130223, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34088099

RESUMO

Intercropping technology is applied widely in crop cultivation to help remediate soil polluted with heavy metals. To investigate the feasibility and potential of intercropping hyperaccumulator plants with crops in cadmium (Cd)- and zinc (Zn)-contaminated soil, a pot experiment was conducted to examine plant growth and the contents of Cd and Zn in the soil following intercropping of wheat and Sedum plumbizincicola. Five treatments were examined: control (wheat monoculture: 36 seedlings per pot), and intercropping of wheat with different planting densities of S. plumbizincicola (3, 6, 9 and 15 seedlings per pot, respectively). Results showed a decrease in soil pH, and in soil and wheat contents of Cd and Zn with increasing planting density of S. plumbizincicola, while the removal rate of Cd and Zn increased. Meanwhile, excessive planting (15 seedlings per pot) inhibited wheat growth by 27.34% compared with the control, and overall, the optimal planting density was 9 seedlings per pot, resulting in effective remediation with only a moderate effect on wheat growth. These findings highlight the value of intercropping S. plumbizincicola with wheat as a means of improving remediation of soil contaminated with heavy metals (Cd and Zn).


Assuntos
Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Solo , Poluentes do Solo/análise , Triticum , Zinco/análise
10.
J Hazard Mater ; 417: 125955, 2021 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-33975168

RESUMO

Cadmium (Cd) is harmful to the environment and threatens human health. With the increasing use of cerium oxide nanoparticles (CeO2NPs) in extensive industries, investigating the combination of CeO2NPs and plants has attracted research interests for phytoremediation. Here, we explored the effects of CeO2NPs on Cd uptake, transport and the consequent Cd accumulation in Sedum alfredii. Exposure of 50 or 500 mg L-1 CeO2NPs alone had no apparent damaging effects on plant growth. However, upon Cd condition, the consistent CeO2NPs decreased Cd concentrations in the roots and shoots by up to 37%. Furthermore, the application of a metabolic inhibitor revealed that CeO2NPs mainly decreased the Cd uptake in roots by the apoplastic pathway. Simultaneously, CeO2NPs accelerated the development of Casparian strips (CSs) and suberin, which was further proven by the elevated expression levels of genes associated with their formation, SaCASP, SaGPAT5, SaKCS20 and SaCYP86A1. Compared to CeO2NPs added alone, the concurrent Cd decreased the Ce contents in the roots and altered its translocation from root to shoot. Taken together, both CeO2NPs and Cd influence the interactional uptake of both chemicals in roots of S. alfredii mainly via the apoplastic pathway which is primarily regulated by the development of CSs and suberin.


Assuntos
Nanopartículas , Sedum , Cádmio/toxicidade , Cério , Humanos , Raízes de Plantas
11.
J Hazard Mater ; 417: 125977, 2021 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-33992011

RESUMO

Decapitation and root pruning, can impact plant morphological and physiological characteristics, which may determine the efficiency of phytoremediation. However, the effects of decapitated and root-pruned plants on the characterization of dissolved organic matter (DOM) and enzymatic activity, which determine the bioavailability of soil pollutants, have rarely been reported. This study aims to characterize DOM and enzymatic activity in the rhizosphere soil of Sedum alfredii when treated by decapitation and root pruning. Decapitation, slight pruning (10% root cutting), and their combination stimulated S. alfredii to secrete more DOM in the rhizosphere soil compared with the control. Furthermore, the proportions of hydrophilic increased from 42.7% in the control to 57.1% in the decapitation and slight pruning combination. Soil urease, invertase, and neutral phosphatase activities were higher in the rhizosphere soil of decapitated and root-pruned S. alfredii, and the highest values were observed with their combination. DOM from the soils of decapitated and root-pruned S. alfredii had significantly higher Cd extraction ability compared with that of the untreated species. Based on the findings of this study, we suggest that decapitation and root pruning can improve the phytoremediation efficiency of S. alfredii by increasing the bioavailability of Cd in its rhizosphere.


Assuntos
Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Raízes de Plantas/química , Rizosfera , Solo , Poluentes do Solo/análise
12.
Zhongguo Zhong Yao Za Zhi ; 46(10): 2474-2480, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34047093

RESUMO

To explore the effect of light intensity in cultivating environment on the hepetoprotective activity of Sedum sarmentosum, S. sarmentosum were planted under five water treatments for 60 days, namely 100% full sunlight(G1), 77% full sunlight(G2), 60% full sunlight(G3), 38% full sunlight(G4), and 16% full sunlight(G5) and CCl_4 drug-induced liver injury model in vitro was used. Cell viability, cell cycle, and cell apoptosis were individually detected by MTT, PI single staining, and Annexin-V FITC/PI double staining assays. Additionally, ALT, AST and antioxidant index in supernatant were determined by colorimetry. And the relationship among the protective effects, chemical composition and antioxidant activity were also analyzed. The results showed that S. sarmentosum aqueous extract could significantly improve the HepG2 cell viability. Among the five S. sarmentosum groups, the cell viability of G1(100% full sunlight) treatment was the highest, and the cell apoptosis was the least. Meanwhile, the level of ALT, AST, and MDA in G1 was the lowest, but it achieved the highest level of SOD and GSH. Moderate light shading(60% full light) also improved the effect of protecting liver and reducing the enzyme. It was found that cell viability was positively correlated with ferricion reducing capacity. ALT activity was positively correlated with isorhamnetin content. Taken together, different light intensity had great influence on hepatoprotective effect of S. sarmentosum, which may be related to its antioxidant capacity. From the perspective of hepetoprotective activity, S. sarmentosum should be planted under full light.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Sedum , Antioxidantes , Células Hep G2 , Humanos , Fígado , Extratos Vegetais/farmacologia , Água
13.
J Photochem Photobiol B ; 218: 112182, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33813366

RESUMO

The fluorescence signals emitted by chlorophyll molecules of plants is a promising non-destructive indicator of plant physiology due to its close link to photosynthesis. In this work, a deep photophysical study of chlorophyll fluorescence was provided, to assess the sub-optimal illumination effects on three plant species: L. sativa, A. hybridus and S. dendroideum. In all the cases, low light (LL) treatment induced an increase in pigment content. Fluorescence ratios - corrected by light reabsorption processes - remained constant, which suggested that photosystems stoichiometry was conserved. For all species and treatments, quantum yields of photophysical decay remained around 0.2, which meant that the maximum possible photosynthesis efficiency was about 0.8. L. sativa (C3) acclimated to low light illumination, displayed a strong increase in the LHC size and a net decrease in the photosynthetic efficiency. A. hybridus (C4) was not appreciably stressed by the low light availability whereas S. dendroideum (CAM), decreased its antenna and augmented the quantum yield of primary photochemistry. A novel approach to describe NPQ relaxation kinetics was also presented here and used to calculate typical deactivation times and amplitudes for NPQ components. LL acclimated L. sativa presented a much larger deactivation time for its state-transition-related quenching than the other species. Comprehensive fluorescence analysis allowed a deep study of the changes in the light-dependent reactions of photosynthesis upon low light illumination treatment.


Assuntos
Amaranthus/química , Clorofila/química , Corantes Fluorescentes/química , Alface/química , Extratos Vegetais/química , Sedum/química , Cinética , Iluminação , Processos Fotoquímicos , Fotossíntese , Folhas de Planta/química
14.
Ecotoxicol Environ Saf ; 217: 112240, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33901783

RESUMO

Quorum sensing (QS) plays an important role in the intensive communication between plants and microbes in the rhizosphere during the phytoremediation. This study explored the influence of the root exudates of hyperaccumulator Sedum alfredii on Pseudomonas aeruginosa based on QS. The effects of the components of root exudates, genes expression and transcription regulation of QS system (especially the las system) in Pseudomonas aeruginosa wild-type strain (WT) and rhl system mutant strain (ΔrhlI) were systematically analyzed and discussed. The WT and ΔrhlI exposed to gradient root exudates (0×, 1×, 2×, 5× and 10×) showed a concentration-corrective inhibition on protease production, with the inhibition rates of 51.4-74.5% and 31.2-50.0%, respectively. Among the components of the root exudates of Sedum alfredii, only thymol had an inhibition effects to the root exudates on the activity of protease and elastase. The inhibition rates of 50 µmol/L thymol on protease and elastase in WT were 44.7% and 24.3%, respectively, which was consistent with the variation in ΔrhlI. The gene expression of lasB declined 36.0% under the 1× root exudate treatment and 73.0% under the 50 µmol/L thymol treatment. Meanwhile, there was no significant impact on N-3-oxo-dodecanoyl-L-homoserine lactone signal production and the gene expression of lasI and lasR. Therefore, thymol from Sedum alfredii root exudates could inhibit the formation of protease and elastase in Pseudomonas aeruginosa by suppressing the expression of lasB, without any significant influence on the main las system as a potential natural QS inhibitor.


Assuntos
Exsudatos de Plantas/toxicidade , Pseudomonas aeruginosa/efeitos dos fármacos , Percepção de Quorum/efeitos dos fármacos , Sedum , 4-Butirolactona/análogos & derivados , Proteínas de Bactérias/metabolismo , Exsudatos e Transudatos/metabolismo , Regulação Bacteriana da Expressão Gênica , Pseudomonas aeruginosa/metabolismo
15.
Int J Biol Macromol ; 180: 152-160, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33741368

RESUMO

Sedum sarmentosum Bunge (SS) is clinically used as Chinese medicine for hepatitis related diseases treatment. The purpose of this study was to explore the chemical structures of polysaccharides from this plant. A neutral polysaccharide (SSWP) was isolated and purified by ion-exchange chromatography and Superdex-75 column. The obtained SSWP was a homogenous one with a molecular weight of 21.5 kDa according to the high-performance gel permeation chromatography. The major monosaccharide composition of SSWP was arabinose, glucose and galactose in a molar ratio of 2.4:1:1.8. The methylation analysis showed that SSWP consists mainly of Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, →4)-Galp-(1→, →4)-Glcp-(1→. The NMR result and enzymatic digestion data comprehensively indicated that SSWP was a novel arabinogalactoglucan-type structure. The anticancer assay in vitro exhibited that SSWP could effectively inhibit 48.9% of Huh-7 cells growth at 50 µg/mL and arrest cells at S-phase, and induce tumor cells apoptosis. Together, polysaccharide from S. sarmentosum Bunge could be a potential natural antitumor agent.


Assuntos
Antineoplásicos/química , Carcinoma Hepatocelular/metabolismo , Proliferação de Células/efeitos dos fármacos , Glucanos/química , Glucanos/farmacologia , Neoplasias Hepáticas/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Sedum/química , Apoptose/efeitos dos fármacos , Arabinose/análise , Carcinoma Hepatocelular/patologia , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Galactose/análise , Humanos , Neoplasias Hepáticas/patologia , Peso Molecular
16.
Environ Pollut ; 278: 116837, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33706243

RESUMO

Sedum alfredii is a Cd/Zn hyperaccumulator native to China, which was collected from a mined area where Mn content in soil was extremely high, together with Zn and Cd content. We investigated the tolerance and accumulation ability of Mn and its possible association with Cd hyperaccumulation in this plant species by using MP-AES, SR-µ-XRF, and RT-PCR. The results showed that the hyperaccumulating ecotype (HE) S. alfredii exhibited high tolerance to Mn and accumulating around 10,000 and 12,000 mg kg-1 Mn in roots and shoots, respectively, without exhibiting toxicity under 5000 mg kg-1 Mn treatment for 4 weeks. Exposure to Cd significantly reduced plant uptake of Mn. In contrast, exogenous Mn application significantly improved root uptake and root-to-shoot translocation of Cd, resulting in the increased Cd accumulation in the shoots of HE S. alfredii. SR-µ-XRF analysis demonstrated that high Mn (20 µM) exposure resulted in higher intensities of Cd localized in both stem vascular bundles and cortex, as well as leaf mesophyll cells, than in those treated with low Mn levels (0.2 µM or 2.0 µM). RT-PCR analysis of several genes possibly involved in Mn/Cd transportation showed that expression of SaNramp3 in roots was significantly reduced under high Mn exposure. These results suggested a significant interaction between Cd and Mn in the HE S. alfredii plants, possibly through their competition for transporters and theoretically provided a strategy to improve the efficiency of Cd extraction from polluted soils by this plant species, after using appropriate nutrient management of Mn.


Assuntos
Sedum , Poluentes do Solo , Cádmio , China , Raízes de Plantas/química , Poluentes do Solo/análise , Poluentes do Solo/toxicidade , Zinco
17.
Int J Mol Sci ; 22(3)2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33525549

RESUMO

Lateral root (LR) formation promotes plant resistance, whereas high-level ethylene induced by abiotic stress will inhibit LR emergence. Considering that local auxin accumulation is a precondition for LR generation, auxin-induced genes inhibiting ethylene synthesis may thus be important for LR development. Here, we found that auxin response factor 4 (SaARF4) in Sedum alfredii Hance could be induced by auxin. The overexpression of SaARF4 decreased the LR number and reduced the vessel diameters. Meanwhile, the auxin distribution mode was altered in the root tips and PIN expression was also decreased in the overexpressed lines compared with the wild-type (WT) plants. The overexpression of SaARF4 could reduce ethylene synthesis, and thus, the repression of ethylene production decreased the LR number of WT and reduced PIN expression in the roots. Furthermore, the quantitative real-time PCR, chromatin immunoprecipitation sequencing, yeast one-hybrid, and dual-luciferase assay results showed that SaARF4 could bind the promoter of 1-aminocyclopropane-1-carboxylate oxidase 4 (SaACO4), associated with ethylene biosynthesis, and could downregulate its expression. Therefore, we concluded that SaARF4 induced by auxin can inhibit ethylene biosynthesis by repressing SaACO4 expression, and this process may affect auxin transport to delay LR development.


Assuntos
Aminoácido Oxirredutases/genética , Ácidos Indolacéticos/farmacologia , Sedum/crescimento & desenvolvimento , Fatores de Transcrição/metabolismo , Imunoprecipitação da Cromatina , Etilenos/biossíntese , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Sedum/efeitos dos fármacos , Sedum/genética , Sedum/metabolismo , Fatores de Transcrição/genética
18.
Sci Rep ; 11(1): 3023, 2021 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-33542339

RESUMO

The F-box genes, which form one of the largest gene families in plants, are vital for plant growth, development and stress response. However, F-box gene family in Sedum alfredii remains unknown. Comprehensive studies addressing their function responding to cadmium stress is still limited. In the present study, 193 members of the F-box gene (SaFbox) family were identified, which were classified into nine subfamilies. Most of the SaFboxs had highly conserved domain and motif. Various functionally related cis-elements involved in plant growth regulation, stress and hormone responses were located in the upstream regions of SaFbox genes. RNA-sequencing and co-expression network analysis revealed that the identified SaFbox genes would be involved in Cd stress. Expression analysis of 16 hub genes confirmed their transcription level in different tissues. Four hub genes (SaFbox40, SaFbox51, SaFbox136 and SaFbox170) were heterologously expressed in a Cd-sensitive yeast cell to assess their effects on Cd tolerance. The transgenic yeast cells carrying SaFbox40, SaFbox51, SaFbox136, or SaFbox170 were more sensitive and accumulated more cadmium under Cd stress than empty vector transformed control cells. Our results performed a comprehensive analysis of Fboxs in S. alfredii and identified their potential roles in Cd stress response.


Assuntos
Proteínas F-Box/genética , Sedum/genética , Poluentes do Solo/toxicidade , Transcriptoma/genética , Biodegradação Ambiental/efeitos dos fármacos , Cádmio/toxicidade , Proteínas F-Box/classificação , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Proteínas de Plantas/genética , Sedum/efeitos dos fármacos , Sedum/crescimento & desenvolvimento , Estresse Fisiológico/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos
19.
Int J Phytoremediation ; 23(10): 1052-1060, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33491471

RESUMO

Sedum plumbizincicola (S. plumbizincicola) is known as a sufficient plant for phytoremediation of cadmium (Cd) polluted soils. This study aimed to investigate the effects of ethylene diamine tetraacetic acid (EDTA), tea saponin (TS), and citric acid (CA) on Cd uptake and translocation by S. plumbizincicola. To do so, using a pot experiment, we set four concentration levels of activators (1, 3, 5, and 10 mmol L-1) and a control (CK). Results showed that none of the applied activators had significant impact on soil pH. Except for CA-10, the concentration of available Cd in Cd polluted soils increased by 65.8-72.9% compared with CK. The EDTA-1, CA-1, and TS-5 treatments caused significant increases of 52.3, 67.2, and 38.4%, respectively, in the biomass of aerial parts of S. plumbizincicola (p < 0.05) compared with CK. Except for CA-3, activators increased Cd accumulation in the aerial parts of plants by 47-124% compared with CK. Of all activators, EDTA-3 caused the highest Cd accumulation of 6.64 g pot-1 in the aerial plant tissues followed by CA-10 (6.25 g pot-1) and TS-1 (5.48 g pot-1). Finally, our results suggested that the application of S. plumbizincicola together with different activators sufficiently reduced soil total Cd by 4.64-48.4% compared with CK. These findings suggest that appropriate application of EDTA, TS, and CA can promote phytoremediation of Cd contaminated soils by hyper-accumulators. In particular, the combined use of EDTA and S. plumbizincicola is an affordable and promising strategy for remediation of Cd contaminated soil.Novelty statement: Sedum plumbizincicola (S. plumbizincicola) is a well-known hyper-accumulator plant for remediation of cadmium (Cd) and zinc (Zn) contaminated soils. In addition, low molecular rganic acids and macromolecular chelating agents can improve the solubility and leaching of soil heavy metals. In the present work, we examined the combined effects of three activators (EDTA, tea saponin, and citric acid) with S. plumbizincicola to remediate a Cd contaminated soil in Anhui Province, East China. Our results indicated the effectiveness of these activators to increase soil available Cd, as well as improving the biomass of S. plumbizincicola and its Cd uptake. We believe that this study provides an efficient approach to increase the uptake of Cd by S. plumbizincicola, restoring Cd contaminated soils. Nevertheless, excessive activators may have adverse effects on soil aggregates and soil microorganisms. Therefore, it is necessary to control the amount of chelating agents and subsequently the deterioration of soil quality.


Assuntos
Saponinas , Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , China , Ácido Cítrico , Ácido Edético , Solo , Poluentes do Solo/análise , Chá
20.
Planta ; 253(1): 12, 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33389204

RESUMO

MAIN CONCLUSION: Cadmium-sensitive yeast screening resulted in the isolation of protein translation factor SaeIF1 from the hyperaccumulator Sedum alfredii which has both general and special regulatory roles in controlling cadmium accumulation. The hyperaccumulator of Sedum alfredii has the extraordinary ability to hyperaccumulate cadmium (Cd) in shoots. To investigate its underlying molecular mechanisms of Cd hyperaccumulation, a cDNA library was generated from leaf tissues of S. alfredii. SaeIF1, belonging to the eukaryotic protein translation factor SUI1 family, was identified by screening Cd-sensitive yeast transformants with this library. The full-length cDNA of SaeIF1 has 582 bp and encodes a predicted protein with 120 amino acids. Transient expression assays showed subcellular localization of SaeIF1 in the cytoplasm. SaeIF1 was constitutively and highly expressed in roots and shoots of the hyperaccumulator of S. alfredii, while its transcript levels showed over 100-fold higher expression in the hyperaccumulator of S. alfredii relative to the tissues of a nonhyperaccumulating ecotype of S. alfredii. However, the overexpression of SaeIF1 in yeast cells increased Cd accumulation, but conferred more Cd sensitivity. Transgenic Arabidopsis thaliana expressing SaeIF1 accumulated more Cd in roots and shoots without changes in the ratio of Cd content in shoots and roots, but were more sensitive to Cd stress than wild type. Both special and general roles of SaeIF1 in Cd uptake, transportation, and detoxification are discussed, and might be responsible for the hyperaccumulation characteristics of S. alfredii.


Assuntos
Sedum , Cádmio/metabolismo , Ecótipo , Folhas de Planta/genética , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Sedum/genética , Sedum/metabolismo , Poluentes do Solo/metabolismo
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