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3.
Sci Rep ; 13(1): 867, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650283

RESUMO

We investigated a method for automatic skin tissue characterization based on optical coherence tomography (OCT) imaging. We developed a manually scanned single fiber OCT instrument to perform in vivo skin imaging and tumor boundary assessment. The goal is to achieve more accurate tissue excision in Mohs micrographic surgery (MMS) and reduce the time required for MMS. The focus of this study was to develop a novel machine learning classification method to automatically identify abnormal skin tissues through one-class classification. We trained a deep convolutional neural network (CNN) with a U-Net architecture for automatic skin segmentation, used the pre-trained U-Net as a feature extractor, and trained one-class support vector machine (SVM) classifiers to detect abnormal tissues. The novelty of this study is the use of a neural network as a feature extractor and the use of a one-class SVM for abnormal tissue detection. Our approach eliminated the need to engineer the features for classification and eliminated the need to train the classifier with data obtained from abnormal tissues. To validate the effectiveness of the one-class classification method, we assessed the performance of our algorithm using computer synthesized data, and experimental data. We also performed a pilot study on a patient with skin cancer.


Assuntos
Pele , Tomografia de Coerência Óptica , Humanos , Tomografia de Coerência Óptica/métodos , Projetos Piloto , Pele/diagnóstico por imagem , Redes Neurais de Computação , Aprendizado de Máquina , Máquina de Vetores de Suporte
4.
J Med Case Rep ; 17(1): 18, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36653860

RESUMO

BACKGROUND: Dermatofibrosarcoma protuberans (DFSP) is a rare variant of skin sarcoma which is characterized by proliferation of spindle cells in a storiform pattern. Although it is mostly benign in its primary stages, it can cause a high burden of morbidity unless it is thoroughly excised. CASE PRESENTATION: Here, we review six cases of DFSP which were characterized by skin lesions in various parts of the body. Patients were from 26 to 51 years old; four were Asian men and two were Asian women. Wide surgical excision was performed for all these patients and no extra treatment was considered. Samples were studied by hematoxylin and eosin (H&E) staining and immunohistochemical (IHC) tests. Only one of our patients experienced recurrence after the initial surgery. CONCLUSION: Determining the best surgical method is still a dilemma in the treatment of DFSP lesions. There are numerous studies to prove the efficacy of various surgical interventions. Although DFSP is not commonly known as a malignant skin lesion, delay in treatment will have a catastrophic impact on patients' lives. Thus, applying an in-time surgical method (wide local excision in our cases) in treating DFSP is crucial in preventing recurrence as well as decreasing the morbidity burden of DFSP.


Assuntos
Dermatofibrossarcoma , Neoplasias Cutâneas , Masculino , Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Dermatofibrossarcoma/cirurgia , Dermatofibrossarcoma/patologia , Pele/patologia , Neoplasias Cutâneas/cirurgia , Neoplasias Cutâneas/patologia , Recidiva Local de Neoplasia/cirurgia , Recidiva Local de Neoplasia/patologia , Imuno-Histoquímica
6.
Skin Therapy Lett ; 28(1): 1-6, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36657434

RESUMO

Atopic dermatitis (AD) is a common, chronic, recurrent, immune-mediated inflammatory skin disease. Targeted treatment options remain limited. Tralokinumab (Adtralza®) is a promising, new systemic therapy that inhibits interleukin-13. It was recently approved by Health Canada and the US FDA for the treatment of moderate-to-severe AD in adults and may be used alone or with topical corticosteroids. Herein, we review the efficacy and safety of tralokinumab in adults, as demonstrated in clinical trials.


Assuntos
Dermatite Atópica , Humanos , Adulto , Dermatite Atópica/tratamento farmacológico , Resultado do Tratamento , Anticorpos Monoclonais , Pele , Índice de Gravidade de Doença
7.
Lasers Med Sci ; 38(1): 45, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36658259

RESUMO

Picosecond lasers have a very short pulse duration and a high peak power density. When fractional optical delivery systems are attached to picosecond lasers, they generate an array of concentrated microspots with a high fluence surrounded by areas with a low fluence. This article discusses the histologic characteristics and clinical applications of fractional picosecond laser treatment. Fractional picosecond laser produces laser-induced optical breakdown (LIOB) and laser-induced cavitation (LIC) in the epidermis and dermis respectively, and can encourage skin regeneration and dermal remodeling. It has been shown that fractional picosecond laser has a positive effect on facial photoaging, enlarged facial pores, dyspigmentation, wrinkles, and atrophic scars. Further research is still needed to confirm the benefits of fractional picosecond lasers.


Assuntos
Lasers de Estado Sólido , Transtornos da Pigmentação , Humanos , Lasers de Estado Sólido/uso terapêutico , Pele/patologia , Epiderme/patologia , Cicatriz/patologia , Transtornos da Pigmentação/patologia , Resultado do Tratamento
8.
Am J Case Rep ; 24: e937843, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36659828

RESUMO

BACKGROUND Cutaneous metastatic breast cancer is a rare manifestation. Causes include vascular or lymphatic spread or iatrogenic mechanisms following surgery. A sub-type of this disease process, "Carcinoma Erysipeloides," represents subcutaneous and dermal tissue layer invasion via lymphatic spread. Diagnosis can be challenging, and therefore, obtaining a thorough history and physical, with careful inspection of prior surgical scars is essential for an accurate diagnosis. Lesions present in variable ways, including papules, plaques, ulcerations, nodules, crusting, or fungating masses, with common locations in the chest, scalp, abdomen, and less commonly the arms. When carcinoma erysipeloides is identified, it is imperative to evaluate for distant metastases. Recent literature has identified benefits with trastuzumab deruxtecan therapy instead of trastuzumab emtansine, with decreased progression rates and decreased mortality rates. Metastasis to the skin can indicate advanced disease; however, this metastatic site may be preferable to visceral organs or bones in terms of prognosis. CASE REPORT We present a rare manifestation of metastatic breast cancer in 45-year-old Hispanic woman, status post neoadjuvant chemotherapy and radical cystectomy on maintenance trastuzumab and pertuzumab. We discuss the clinical presentation variability, keys to diagnosis, treatment considerations, and outcomes for this unique patient population. CONCLUSIONS Carcinoma erysipeloides varies in clinical presentation, especially when patients develop exclusive skin lesions. We identify common etiologies for this progression of disease and discuss combination therapy which has demonstrated a reduction in mortality in this patient population.


Assuntos
Neoplasias da Mama , Carcinoma , Neoplasias Cutâneas , Feminino , Humanos , Pessoa de Meia-Idade , Neoplasias da Mama/terapia , Neoplasias da Mama/patologia , Ado-Trastuzumab Emtansina/uso terapêutico , Neoplasias Cutâneas/patologia , Carcinoma/tratamento farmacológico , Pele/patologia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
9.
Molecules ; 28(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36677948

RESUMO

As an alternative to fossil volatile hydrocarbon solvents used nowadays in perfumery, investigation on essential oil of Commiphora wildii Merxm. oleo gum resin as a source of heptane is reported here. Heptane, representing up to 30 wt-% of this oleo gum resin, was successfully isolated from the C. wildii essential oil, using an innovative double distillation process. Isolated heptane was then used as a solvent in order to extract some noble plants of perfumery. It was found that extracts obtained with this solvent were more promising in terms of sensory analysis than those obtained from fossil-based heptane. In addition, in order to valorize the essential oil depleted from heptane, chemical composition of this oil was found to obtain, and potential biological activity properties were studied. A total of 172 different compounds were identified by GC-MS in the remaining oil. In vitro tests-including hyaluronidase, tyrosinase, antioxidant, elastase and lipoxygenase, as well as inhibitory tests against two yeasts and 21 bacterial strains commonly found on the skin-were carried out. Overall, bioassays results suggest this heptane-depleted essential oil is a promising active ingredient for cosmetic applications.


Assuntos
Óleos Voláteis , Óleos Voláteis/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Commiphora/química , Pele , Resinas Vegetais
10.
Molecules ; 28(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36677946

RESUMO

Type I and V collagens are the major components of fibrillogenic proteins in fish skin, and their hydrolysis products possess hyaluronidase inhibitory activity. In this study, for the first time, type I and V collagens were isolated from the skin of shortbill spearfish and striped marlin. Type I (2α1[I]α2[I]) and type V (α1[V]α3[V]α2[V]) collagens composed of distinct α-peptide chains with comparable structures were investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and UV spectrophotometric chromatography. After enzymatic digestion, the collagen peptides were purified by using ultrafiltration (30 KDa) and high-performance liquid chromatography (RP-HPLC) to yield CPI-F3 and CPV-F4 fractions with strong hyaluronidase inhibition rates (42.17% and 30.09%, respectively). Based on the results of simulated gastrointestinal fluid, temperature, and pH stability assays, CPI-F3 and CPV-F4 exhibited stability in gastric fluid and showed no significant changes under the temperature range from 50 to 70 °C (p > 0.05). The results of this first research on the bioactivity of type V collagen peptides provide valuable information for the biomedical industry and show the potential for future bioactivity investigations of type V collagen and its peptides.


Assuntos
Colágeno Tipo V , Hialuronoglucosaminidase , Animais , Colágeno Tipo V/análise , Colágeno/química , Peptídeos/farmacologia , Peptídeos/análise , Peixes/metabolismo , Pele/metabolismo , Eletroforese em Gel de Poliacrilamida
11.
Molecules ; 28(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36677965

RESUMO

Peptides, functional nutrients with a size between those of large proteins and small amino acids, are easily absorbed by the human body. Therefore, they are seeing increasing use in clinical medicine and have revealed immunomodulatory and anti-inflammatory properties which could make them effective in healing skin wounds. This review sorted and summarized the relevant literature about peptides during the past decade. Recent works on the extraction, modification and synthesis of peptides were reviewed. Importantly, the unique beneficial effects of peptides on the skin were extensively explored, providing ideas for the development and innovation of peptides and laying a knowledge foundation for the clinical application of peptides.


Assuntos
Peptídeos , Pele , Humanos , Peptídeos/farmacologia , Peptídeos/química , Proteínas/farmacologia , Cicatrização , Aminoácidos/farmacologia
12.
Sensors (Basel) ; 23(2)2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36679411

RESUMO

Photoplethysmography (PPG) is a noninvasive optical technology with applications including vital sign extraction and patient monitoring. However, its current use is primarily limited to heart rate and oxygenation monitoring. This study aims to demonstrate the utility of PPG for physiological investigations. In particular, we sought to demonstrate the utility of simultaneous data acquisition from several regions of tissue using remote/contactless PPG (rPPG). Specifically, using a high-speed scientific-grade camera, we collected rPPG from the hands (palmar/dorsal) of 22 healthy volunteers. Data collected through the red and green channels of the RGB CMOS sensor were analyzed. We found a statistically significant difference in the amplitude of the glabrous skin signal over the non-glabrous skin signal (1.41 ± 0.85 in the red channel and 2.27 ± 0.88 in the green channel). In addition, we found a statistically significant lead of the red channel over the green channel, which is consistent between glabrous (17.13 ± 10.69 ms) and non-glabrous (19.31 ± 12.66 ms) skin. We also found a statistically significant lead time (32.69 ± 55.26 ms in the red channel and 40.56 ± 26.97 ms in the green channel) of the glabrous PPG signal over the non-glabrous, which cannot be explained by bilateral variability. These results demonstrate the utility of rPPG imaging as a tool for fundamental physiological studies and can be used to inform the development of PPG-based devices.


Assuntos
Fotopletismografia , Pele , Humanos , Fotopletismografia/métodos , Monitorização Fisiológica , Frequência Cardíaca/fisiologia , Voluntários Saudáveis , Processamento de Sinais Assistido por Computador
13.
Sensors (Basel) ; 23(2)2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36679603

RESUMO

Previous research has demonstrated the potential to reconstruct human facial skin spectra based on the responses of RGB cameras to achieve high-fidelity color reproduction of human facial skin in various industrial applications. Nonetheless, the level of precision is still expected to improve. Inspired by the asymmetricity of human facial skin color in the CIELab* color space, we propose a practical framework, HPCAPR, for skin facial reflectance reconstruction based on calibrated datasets which reconstruct the facial spectra in subsets derived from clustering techniques in several spectrometric and colorimetric spaces, i.e., the spectral reflectance space, Principal Component (PC) space, CIELab*, and its three 2D subordinate color spaces, La*, Lb*, and ab*. The spectra reconstruction algorithm is optimized by combining state-of-art algorithms and thoroughly scanning the parameters. The results show that the hybrid of PCA and RGB polynomial regression algorithm with 3PCs plus 1st-order polynomial extension gives the best results. The performance can be improved substantially by operating the spectral reconstruction framework within the subset classified in the La* color subspace. Comparing with not conducting the clustering technique, it attains values of 25.2% and 57.1% for the median and maximum errors for the best cluster, respectively; for the worst, the maximum error was reduced by 42.2%.


Assuntos
Algoritmos , Pele , Humanos , Cor , Colorimetria/métodos , Face/fisiologia
14.
Sensors (Basel) ; 23(2)2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36679716

RESUMO

Pressure injuries (PI) result from pressure-induced damage to the skin and underlying tissues. Currently, Stage I PI are detected using visual skin assessments. However, this visual method is unable to detect skin color changes in persons with darkly pigmented skin, which results in a higher Stage II-IV PI incidence and PI-associated mortality in persons with a darker complexion. Thus, a more objective method of early-stage PI detection is of great importance. Optical spectroscopy is a promising modality for the noncontact diagnosis and monitoring of skin water content, capable of detecting edema and Stage I PI. The scope of the current study is to assess the feasibility of imaging the water content of the skin using Si-based sensors. We have considered two primary cases: the elevated bulk water content (edema) and localized water pool (e.g., blood vessels). These two cases were analyzed using analytical models. We found that detecting the watercontent contrast associated with edema in tissues is within the reach of Si-based sensors. However, although the effect is expected to be detectable even with consumer-grade cameras, with the current state of technologies, their use in real-world conditions faces numerous technical challenges, mainly due to the narrow dynamic range.


Assuntos
Lesão por Pressão , Humanos , Lesão por Pressão/diagnóstico , Água , Estudos de Viabilidade , Pele/diagnóstico por imagem , Edema
15.
Sensors (Basel) ; 23(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36679807

RESUMO

Aesthetic medicine is a dynamically developing field of medicine. It has an impact not only on the improvement of the external appearance, but also on health and quality of life. Currently, vascular changes affect many patients and significantly diminish the condition of the skin. The development of modern laser therapy has contributed to the successful management of multiple skin conditions, among them vascular lesions. The aim of our study was to show the efficacy of repetitive 532 nm laser therapy in reducing vascular skin lesions located on the facial area. Observations were possible due to the implementation of System of Skin Analysis. We retrospectively analyzed the records of 120 patients (100 women and 20 men) using "VISIA" Skin Analysis System after 532 nm laser therapy. Treatment with the use of the 532 nm vascular laser turned out to be effective in reducing vascular changes in both women and men. The skin phototypes did not significantly affect the therapy efficacy. Neither the age of patients nor number of laser sessions affect therapy efficacy. 532 nm laser therapy is effective in reducing vascular skin lesions located in the face area.


Assuntos
Qualidade de Vida , Dermatopatias , Masculino , Humanos , Feminino , Resultado do Tratamento , Estudos Retrospectivos , Pele , Lasers
16.
Arthritis Res Ther ; 25(1): 12, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36698198

RESUMO

BACKGROUND: Systemic sclerosis is a rheumatoid disease best known for its fibrotic skin manifestations called scleroderma. Alternatively activated (M2-type) macrophages are normally involved in the resolution of inflammation and wound healing but also in fibrosing diseases such as scleroderma. TRPA1 is a non-selective cation channel, activation of which causes pain and neurogenic inflammation. In the present study, we investigated the role of TRPA1 in bleomycin-induced skin fibrosis mimicking scleroderma. METHODS: Wild type and TRPA1-deficient mice were challenged with intradermal bleomycin injections to induce a scleroderma-mimicking disease. Macrophages were investigated in vitro to evaluate the underlying mechanisms. RESULTS: Bleomycin induced dermal thickening and collagen accumulation in wild type mice and that was significantly attenuated in TRPA1-deficient animals. Accordingly, the expression of collagens 1A1, 1A2, and 3A1 as well as pro-fibrotic factors TGF-beta, CTGF, fibronectin-1 and YKL-40, and M2 macrophage markers Arg1 and MRC1 were lower in TRPA1-deficient than wild type mice. Furthermore, bleomycin was discovered to significantly enhance M2-marker expression particularly in the presence of IL-4 in wild type macrophages in vitro, but not in macrophages harvested from TRPA1-deficient mice. IL-4-induced PPARγ-expression in macrophages was increased by bleomycin, providing a possible mechanism behind the phenomenon. CONCLUSIONS: In conclusion, the results indicate that interfering TRPA1 attenuates fibrotic and inflammatory responses in bleomycin-induced scleroderma. Therefore, TRPA1-blocking treatment could potentially alleviate M2 macrophage driven diseases like systemic sclerosis and scleroderma.


Assuntos
Esclerodermia Localizada , Escleroderma Sistêmico , Camundongos , Animais , Bleomicina/toxicidade , Ativação de Macrófagos , Interleucina-4/efeitos adversos , Interleucina-4/metabolismo , Escleroderma Sistêmico/patologia , Fibrose , Colágeno/metabolismo , Esclerodermia Localizada/induzido quimicamente , Esclerodermia Localizada/patologia , Modelos Animais de Doenças , Pele/patologia , Canal de Cátion TRPA1/genética
17.
Molecules ; 28(2)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36677659

RESUMO

A wound is the loss of the normal integrity, structure, and functions of the skin due to a physical, chemical, or mechanical agent. Wound repair consists of an orderly and complex process divided into four phases: coagulation, inflammation, proliferation, and remodeling. The potential of natural products in the treatment of wounds has been reported in numerous studies, emphasizing those with antioxidant, anti-inflammatory, and antimicrobial properties, e.g., alkaloids, saponins, terpenes, essential oils, and polyphenols from different plant sources, since these compounds can interact in the various stages of the wound healing process. This review addresses the most current in vitro and in vivo studies on the wound healing potential of natural products, as well as the main mechanisms involved in this activity. We observed sufficient evidence of the activity of these compounds in the treatment of wounds; however, we also found that there is no consensus on the effective concentrations in which the natural products exert this activity. For this reason, it is important to work on establishing optimal treatment doses, as well as an appropriate route of administration. In addition, more research should be carried out to discover the possible side effects and the behavior of natural products in clinical trials.


Assuntos
Produtos Biológicos , Produtos Biológicos/farmacologia , Produtos Biológicos/uso terapêutico , Cicatrização , Pele , Extratos Vegetais/química , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico
18.
Molecules ; 28(2)2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36677909

RESUMO

The potential of recombinant human prolidase (rhPEPD) to induce wound healing in an experimental model of IL-1ß-induced inflammation in human fibroblasts was studied. It was found that rhPEPD significantly increased cell proliferation and viability, as well as the expression of the epidermal growth factor receptor (EGFR) and downstream signaling proteins, such as phosphorylated PI3K, AKT, and mTOR, in the studied model. Moreover, rhPEPD upregulated the expression of the ß1 integrin receptor and its downstream signaling proteins, such as p-FAK, Grb2 and p-ERK 1/2. The inhibition of EGFR signaling by gefitinib abolished rhPEPD-dependent functions in an experimental model of inflammation. Subsequent studies showed that rhPEPD augmented collagen biosynthesis in IL-1ß-treated fibroblasts as well as in a wound healing model (wound closure/scratch test). Although IL-1ß treatment of fibroblasts increased cell migration, rhPEPD significantly enhanced this process. This effect was accompanied by an increase in the activity of MMP-2 and MMP-9, suggesting extracellular matrix (ECM) remodeling during the inflammatory process. The data suggest that rhPEPD may play an important role in EGFR-dependent cell growth in an experimental model of inflammation in human fibroblasts, and this knowledge may be useful for further approaches to the treatment of abnormalities of wound healing and other skin diseases.


Assuntos
Colágeno , Cicatrização , Humanos , Colágeno/metabolismo , Fibroblastos , Inflamação/metabolismo , Receptores ErbB/metabolismo , Modelos Teóricos , Pele
19.
Br J Dermatol ; 188(1): 94-99, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36689507

RESUMO

BACKGROUND: Hyperkeratosis lenticularis perstans (HLP), also known as Flegel disease, is a rare skin disease presenting with asymptomatic small hyperkeratotic papules. The lesions often appear on the dorsal feet and lower legs, and typically develop after the fourth decade of life. A genetic basis for HLP is suspected; however, so far no gene defect linked to the development of HLP has been identified. OBJECTIVES: We aimed to identify the genetic cause of HLP. METHODS: For mutational analysis we studied a cohort of five patients with HLP using next-generation sequencing (NGS). We used DNA -extracted from fresh skin biopsies alongside ethylenediamine tetraacetic acid (EDTA) blood samples from two patients, and formalin-fixed -paraffin-embedded skin biopsy material from three patients. In addition, immunofluorescence staining of HLP lesions from four patients was investigated. RESULTS: In all samples from the five patients with HLP we identified by NGS rare variants in the SPTLC1 gene. In four patients we detected small deletions/frameshift variants and in one patient a splicing variant, predicted to disturb the splicing process. In blood samples the detected variants were heterozygous with an allele frequency of 49% and 50%, respectively. In skin biopsies the allele frequency was within the range of 46-62%. Immunofluorescence staining revealed reduced SPTLC1 protein levels in skin of patients. CONCLUSIONS: Our findings suggest that pathogenic variants in the SPTLC1 gene are the underlying genetic cause of HLP. Of note, the identified variants were either frameshift- or splicing variants probably leading to nonsense-mediated mRNA decay and thus reduced SPTLC1 protein levels. We conclude that diminished SPTLC1, the key enzyme in sphingolipid biosynthesis, leads to the development of HLP, which highlights the sphingolipid pathway as a new therapeutic target.


Assuntos
Ceratose , Humanos , Ceratose/patologia , Pele/patologia , Biópsia/efeitos adversos , Serina C-Palmitoiltransferase
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