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1.
Hum Reprod ; 37(8): 1727-1738, 2022 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-35690928

RESUMO

STUDY QUESTION: Can we identify diurnal oscillations in human semen parameters as well as peak times of semen quality? SUMMARY ANSWER: Human semen parameters show substantial diurnal oscillation, with most parameters reaching a peak between 1100 and 1500 h. WHAT IS KNOWN ALREADY: A circadian clock appears to regulate different physiological functions in various organs, but it remains controversial whether diurnal rhythms occur in human semen parameters. STUDY DESIGN, SIZE, DURATION: The medical record of a provincial human sperm bank (HSB) with 33 430 semen samples collected between 0800 and 1700 h from 1 March 2010 to 8 July 2015 was used to analyze variation in semen parameters among time points. A laboratory study was conducted to collect semen samples (n = 36) from six volunteers at six time points with identical time intervals (2 days plus 4 h) between 6 June and 8 July in 2019, in order to investigate the diurnal oscillation of semen parameters in vivo, with a strictly controlled abstinence period. Therefore, the sperm bank study with a large sample size and the in vivo study with a strictly controlled abstinence period in a 24-h time window could be compared to describe the diurnal rhythms in human semen parameters. PARTICIPANTS/MATERIALS, SETTING, METHODS: Samples were obtained from potential HSB donors and from participants in the laboratory study who were volunteers, recruited by flyers distributed in the community. Total sperm count, sperm concentration, semen volume, progressive motility and total motility were assessed using computer-aided sperm analysis. In addition, sperm chromatin integrity parameters (DNA fragmentation index and high DNA stainability) were assessed by the sperm chromatin structure assay, and sperm viability was measured with flow cytometry in the laboratory study. MAIN RESULTS AND THE ROLE OF CHANCE: The 33 430 samples from the HSB showed a temporal variation in total sperm count, sperm concentration, semen volume, progressive motility and total motility (all P < 0.001) between 0800 and 1700 h. Consequently, the eligibility of semen samples for use in ART, based on bank standards, fluctuated with time point. Each hour earlier/later than 1100 h was associated with 1.14-fold risk of ineligibility. Similarly, the 36 samples taken during the 24-h time window showed diurnal oscillation. With the pre-collection abstinence period strictly controlled, most semen parameters reached the most favorable level between 1100 and 1500 h. LIMITATIONS, REASONS FOR CAUTION: Some of the possible confounding factors, such as energy intake, which might influence semen quality or diurnal rhythms, were not adjusted for in the analyses. In addition, the findings should be considered with caution because the study was conducted in a specific population, time and place, while the timing of oscillations could differ with changing conditions. WIDER IMPLICATIONS OF THE FINDINGS: The findings could help us to estimate semen quality more precisely and to obtain higher quality sperm for use in ART and in natural conception. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by the National Natural Science Foundation of China (81871208) and National Key R&D Program of China (2017YFC1002001). There are no conflicts of interest to declare. TRIAL REGISTRATION NUMBER: N/A.


Assuntos
Análise do Sêmen , Sêmen , Cromatina , Ritmo Circadiano , Humanos , Masculino , Sêmen/fisiologia , Bancos de Esperma , Contagem de Espermatozoides , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia
2.
Zhonghua Nan Ke Xue ; 27(3): 213-218, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-34914302

RESUMO

OBJECTIVE: To investigate the relationship of seminal plasma biochemical indexes with routine semen parameters and that between seminal plasma biochemical indexes. METHODS: Using the automatic biochemical analyzer, we measured the contents of neutral α-glucosidase (NAG), fructose hexokinase (Fru) , citric acid (CA), acid phosphatase (ACP), (zinc) Zn, uric acid (UA), lactase dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (α-HBDH) in the seminal plasma of 84 sperm donors in the Human Sperm Bank of Hebei Province. We analyzed the correlation between these indexes and that between routine semen parameters and these indexes. RESULTS: Sperm concentration and total sperm count were correlated positively with the contents of seminal plasma NAG, ACP, Zn, CA, LDH and α-HBDH (P<0.05) but negatively with Fru (P<0.05), the percentage of progressively motile sperm positively with seminal plasma Zn (P<0.05), and CA positively with NAG, Zn, LDH, α-HBDH and ACP (P<0.01) but negatively with Fru (P<0.01), NAG positively with Zn, LDH, α-HBDH and ACP (P<0.05) but Fru negatively with ACP (P<0.01), Zn positively with LDH, α-HBDH and ACP (P<0.01), and LDH positively with α-HBDH and ACP (P<0.01) but UA negatively with ACP (P<0.05). CONCLUSIONS: Biochemical indexes in the seminal plasma of healthy men are not only closely related to each other, but also to some routine semen parameters.


Assuntos
Líquidos Corporais , Sêmen , Líquidos Corporais/química , Humanos , Masculino , Sêmen/química , Bancos de Esperma , Contagem de Espermatozoides , Espermatozoides , alfa-Glucosidases
3.
Zhonghua Nan Ke Xue ; 27(4): 309-313, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-34914212

RESUMO

OBJECTIVE: To analyze the causes of failure in autologous sperm cryopreservation (ASCP) in the human sperm bank and propose some countermeasures to improve the success rate of ASCP and promote it in human sperm banks. METHODS: We retrospectively analyzed the reasons for and causes of failure in ASCP 1 156 males undergoing ASCP in the Human Sperm Bank of Guangdong Province from January 1, 2016 to December 31, 2019. RESULTS: Of the 1 156 cases of ASCP, 857 (74.1%) succeeded and 299 (25.9%) failed, with a failure rate of 56.1% (96/171) in the microdissection testicular sperm extraction (micro-TESE) group, 29.9% (67/224) in the reproduction insurance group, 21.2% (27/100) in the non-tumor disease group and 17.2% (109/525) in the tumor group, with statistically significant difference between the four groups (χ2 = 109.926, P < 0.01). The causes of failed ASCP included failure to extract semen (14.0% ï¼»42/299ï¼½), failure to meet the standard of sperm cryopreservation (67.6% ï¼»202/299ï¼½), giving up ASCP for low semen quality (7.4% ï¼»22/299ï¼½), and giving up ASCP for some other reasons (11.0% ï¼»33/299ï¼½), including worry about the reliability of cryopreservation technology (6.0% ï¼»18/299ï¼½), suspicion about the complexity of the ASCP procedures (3.0% ï¼»9/299ï¼½) and expectation for fertility recovery after chemotherapy withdrawal (2.0% ï¼»6/299ï¼½). CONCLUSIONS: In view of different causes of failure in ASCP, human sperm banks can provide individualized cryopreservation schemes, including guidance with masturbation or the use of sperm extraction instrument in sperm extraction, strengthening sperm preservation-related education and the awareness of reproduction protection and earlier sperm preservation among cancer patients, promoting the cryopreservation of microsamples of motile sperm by microsample or single sperm freezing, and development of testis tissue cryopreservation to preserve the fertility of children with cancer.


Assuntos
Preservação do Sêmen , Bancos de Esperma , Criança , Humanos , Masculino , Reprodutibilidade dos Testes , Estudos Retrospectivos , Análise do Sêmen , Espermatozoides
4.
Zhonghua Nan Ke Xue ; 27(6): 513-516, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34914290

RESUMO

OBJECTIVE: To analyze the characteristics of the males undergoing autologous sperm preservation (ASP), and provide some reference for human sperm banks to offer targeted service for those undergoing ASP. METHODS: We statistically analyzed the demographic features, reasons for ASP, and other relevant factors of the men applying for ASP in Chongqing from January 2016 to December 2019. RESULTS: Within 4 years, a total of 76 males applied for ASP, of whom about 63.2% came from Chongqing, 52.6% were aged 20-30 years old, 51.3% were undergraduates or above, 36.8% worked in government offices and public institutions, 61.9% were unmarried, nearly 80% acted by the doctor's advice, and 59.2% did it due to diseases, mainly including testis cancer, lymphoma and colon cancer, particularly before radio- or chemotherapy. Testicular sperm extraction (TESE) was successfully performed in 61 (80.3%) of the males. Of the 355 semen samples obtained, 83.1% were found with normal semen parameters, 60.5% cryopreserved successfully, and 4.3% of the frozen samples used for assisted reproduction. CONCLUSIONS: ASP is comparatively a novel practice, with only a few participants. Full use of the aggregation characteristics of the population undergoing ASP should be made, and targeted service provided for the key subjects identified so as to benefit more people in need.


Assuntos
Preservação do Sêmen , Adulto , Criopreservação , Humanos , Masculino , Bancos de Esperma , Espermatozoides , Adulto Jovem
5.
Eur J Obstet Gynecol Reprod Biol ; 260: 198-202, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33839645

RESUMO

OBJECTIVE: To evaluate whether sperm banking is under-used in adolescents with cancer. STUDY DESIGN: We reviewed our 33 years long experience of semen cryopreservation and identified male subjects with cancer aged 14-21 years who banked their sperm. We hypothesized as a referring model a constant rate of referral in every age category (uniform distribution). In addition, we evaluated whether the distribution of the referrals per age varied according to historical periods and compared semen quality in the different age groups. RESULTS: Overall, 317 subjects were selected. A sharp increase with age emerged (p < 0.001): the number of cases augmented from 4 at age 14 years to 79 at age 21 years. This significant increase persisted even when focussing only on subjects older than 16-18 years. An improvement of the distribution of cases per age group emerged when limiting the analysis to the last decade of activity; however, the increase remained significant (p < 0.001). Finally, we investigated whether semen quality differed according to age groups and failed to show marked differences, suggesting that sperm banking even at the youngest ages could be of potential benefit. CONCLUSIONS: This study confirmed that sperm banking is under-used in adolescents with cancers. Future studies should better clarify the determinants of this low referral and identify interventions that can improve the situation.


Assuntos
Neoplasias , Preservação do Sêmen , Adolescente , Adulto , Criopreservação , Humanos , Masculino , Análise do Sêmen , Bancos de Esperma , Espermatozoides , Adulto Jovem
6.
Asian J Androl ; 23(5): 490-494, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33818523

RESUMO

We aimed to compare the sperm quality in different cancer types and benign diseases before gonadotoxic treatment, and assess the usage rate of cryopreserved sperm for assisted reproductive treatment (ART). This retrospective study was conducted at two university clinics between January 2008 and July 2018. A total of 545 patients suffering from cancer or benign diseases were included in the study. The pretreatment sperm analyses were based on the World Health Organization (WHO) guidelines. Patients with testicular malignancy (TM) showed a significantly lower sperm count (median [interquartile range]: 18.7 × 106 [5.3 × 106-43.0 × 106] ml-1; P = 0.03) as well as total sperm count (42.4 × 106 [13.3 × 106-108.5 × 106] per ejaculate; P = 0.007) compared to other malignant and benign diseases. In addition, patients with nonseminomatous TM showed the lowest sperm count (14.3 × 106 [6.0 × 106-29.9 × 106] ml-1, vs seminomas: 16.5 × 106 [4.6 × 106-20.3 × 106] ml-1; P = 0.001). With reference to the WHO 2010 guidelines, approximately 48.0% of the patients with TM and 23.0% with hematological malignancies (HM) had oligozoospermia. During the observation period, only 29 patients (5.3%) used their frozen sperms for 48 ART cycles, resulting in 15 clinical pregnancies and 10 live births. The sperm quality varies with the type of underlying disease, with TM and HM patients showing the lowest sperm counts. Due to the observed low usage rate of cryopreserved sperm, further patient interviews and sperm analyses should be included in the routine oncologic protocols to avoid unnecessary storage expenses. However, sperm banking is worth the effort as it provides hope for men who cannot reproduce naturally after gonadotoxic treatment.


Assuntos
Tratamento Farmacológico/normas , Neoplasias/tratamento farmacológico , Preservação do Sêmen/estatística & dados numéricos , Adulto , Análise de Variância , Tratamento Farmacológico/métodos , Tratamento Farmacológico/estatística & dados numéricos , Humanos , Masculino , Neoplasias/fisiopatologia , Estudos Retrospectivos , Preservação do Sêmen/métodos , Bancos de Esperma/organização & administração , Bancos de Esperma/estatística & dados numéricos , Resultado do Tratamento
7.
Hum Reprod ; 36(5): 1205-1212, 2021 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-33611556

RESUMO

STUDY QUESTION: Is there a relation between the characteristics of potential sperm donors and the acceptance rate of these potential donors? SUMMARY ANSWER: A relatively higher acceptance rate was observed for potential sperm donors who were aged ≤ 35 years, were married, had children, and who had received higher education, and acceptance rates were also higher during spring and winter than summer and autumn. WHAT IS KNOWN ALREADY: Recruiting donors to a sperm bank program is difficult and slow owing to the high rates of rejection and dropout. STUDY DESIGN, SIZE, DURATION: A total of 24040 potential sperm donors were screened by the Henan Human Sperm Bank from 2006 to 2019. PARTICIPANTS/MATERIALS, SETTING, METHODS: Potential sperm donors were recruited using the following baseline requirement: height of 168 cm or taller; age 22-45 years; currently attending or had graduated from high school or above. Men who met the criteria for age, height, and education level were invited for semen quality screening. The acceptable criteria for semen samples included liquefaction time < 60min, volume ≥ 2mL, sperm concentration ≥ 60 × 106/mL, progressive motility ≥ 60%, post-thaw motility ≥ 40%, pre-freezing total motile sperm per vial > 30 × 106/mL, post-thaw total motile sperm per vial > 12 × 106/mL, and freeze-thaw survival rate ≥ 60%. Any potential sperm donors meeting the minimum criteria for acceptable semen quality on two consecutive semen samples were scheduled for clinical assessment, physical examination, and laboratory tests. The reasons for sperm donor rejection were analyzed. The characteristics of accepted and rejected donors were compared using the chi-square test, and multivariate logistic regression analyses were conducted to identify factors associated with the acceptance rate of potential sperm donors and the positive rate of sexually transmitted diseases (STDs). MAIN RESULTS AND THE ROLE OF CHANCE: Only 23.38% (5620/24040) of potential sperm donors were accepted. The top four reasons for rejection were suboptimal semen quality (90.27%), STDs (6.26%), dropped out (2.65%), and chromosomal abnormalities (0.35%). The most common reason for the rejection of donors with an STD was a positive test for mycoplasmas (49.05%), followed by hepatitis B virus (27.56%), Chlamydia trachomatis (4.68%), and Escherichia coli (3.03%). n this study, the acceptance rate for men aged ≤ 35 years was significantly higher than that for men aged >35 years (P < 0.05). The acceptance rates were also significantly higher for men with a higher education than for men with lower education, married men than unmarried men, and men with children than men without children (P < 0.05). Moreover, acceptance rates were significantly higher during spring and winter than during summer (P <0.05) but were not significantly higher during autumn than during summer (P >0.05). LIMITATIONS, REASONS FOR CAUTION: This study was not performed to analyze the effect of lifestyle habits, such as alcohol consumption and cigarette smoking, on the acceptance rate of potential sperm donors. WIDER IMPLICATIONS OF THE FINDINGS: Only a small proportion of potential sperm donors were accepted in this anonymous sperm donor program. New strategies for sperm donor recruitment may be required to improve the acceptance rate. In the future, we may have to target potential sperm donors who are aged ≤ 35 years and who received higher education in order to improve the acceptance rate. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the Joint Construction Project of Henan Medical Science and Technology Research Plan under grant number LHGJ20190389. The authors report no conflict of interest. TRIAL REGISTRATION NUMBER: N/A.


Assuntos
Análise do Sêmen , Bancos de Esperma , Adulto , Criança , China , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides , Adulto Jovem
8.
J Assist Reprod Genet ; 38(6): 1561-1569, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33564937

RESUMO

PURPOSE: Over half of males experience fertility impairment after childhood cancer therapy, which often causes psychosocial distress. Yet, fertility preservation (FP) remains underutilized. The goals of this study were to determine the feasibility and impact of implementing a family-centered FP values clarification tool on sperm banking attempts among adolescent males newly diagnosed with cancer, and identify key determinants of banking attempts. METHODS: A prospective pilot study was conducted among families of males (12-25 years old), prior to cancer therapy. Thirty-nine of 41 families agreed to participate (95%); 98 participants (32 adolescents, 37 mothers, 29 fathers) completed the Family-centered Adolescent Sperm banking values clarification Tool (FAST). Analyses assessed the impact of the FAST on banking attempts and examined associations between demographic/medical characteristics, FAST subscales (perceived threat, benefits, barriers), and banking attempts. RESULTS: Twenty-three (59%) adolescents attempted to bank, compared to 8 adolescents (33%) during baseline assessment (p=.04). Significant associations were identified between banking attempts and adolescents' report of perceived threat (rpb=.45, p=.01) and benefits (rpb=.57, p=.01). Only mothers' proxy reports of adolescent perceived threat (rpb=.42, p=.01) and benefits (rpb=.47, p=.003) were associated with banking attempts, while fathers' self-reported perceived benefits (rpb=.43, p=.03), self-reported barriers (rpb=.49, p=.01), and proxy reports of adolescent perceived threat (rpb=.38, p=.04) and benefits (rpb=.59, p=.02) were associated with banking attempts. CONCLUSION: Adolescent sperm banking attempt rates significantly increased after implementation of a family-centered FP values clarification tool prior to cancer treatment. Findings underscore the importance of targeting both adolescents and their parents, particularly fathers, in FP efforts.


Assuntos
Preservação da Fertilidade , Fertilidade/genética , Preservação do Sêmen , Bancos de Esperma , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Mães , Neoplasias/epidemiologia , Neoplasias/patologia , Estudos Prospectivos , Espermatozoides/crescimento & desenvolvimento , Adulto Jovem
9.
Reprod Biomed Online ; 42(3): 589-594, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33384268

RESUMO

RESEARCH QUESTION: What are the risks associated with cryopreserved semen collected during and after the coronavirus disease 2019 (COVID-19) pandemic wave in Wuhan, China? DESIGN: Retrospective cohort study involving young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank (China) during the pandemic wave (1 January 2020 to 30 January 2020) and after the wave and return to work (7 April 2020 to 30 May 30 2020). One hundred paired semen and blood specimens from 100 donors were included. One-step single-tube nested quantitative real-time polymerase chain reaction (OSN-qRT-PCR) was used to detect SARS-CoV-2. Moreover, to control the unacceptable risk of false-negative results, a second round of screening was performed with pooled RNA from negative semen samples using crystal digital PCR (cd-PCR). RESULTS: For individual blood and semen samples, the target genes, namely the nucleocapsid protein (N) and open reading frame (ORF-1ab) genes, tested negative in all of the 100 paired samples. Further, as per cd-PCR results, there were >20,000 droplets per well in the RNA for each combined sample and no positive droplets were present for either of the aforementioned target genes. A total of 100 paired semen and blood samples from these two groups tested negative for SARS-CoV-2. CONCLUSIONS: Cryopreserved semen at the Hunan Province Human Sperm Bank during and after the COVID-19 pandemic wave was free of SARS-CoV-2 and was judged safe for external use in the future.


Assuntos
COVID-19 , Pandemias , China/epidemiologia , Humanos , Masculino , Reação em Cadeia da Polimerase em Tempo Real , Estudos Retrospectivos , SARS-CoV-2 , Sêmen , Bancos de Esperma , Espermatozoides , Adulto Jovem
10.
Andrology ; 9(1): 204-211, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32814364

RESUMO

BACKGROUND: Previous evidence highlighted that only a minority of men who banked their semen before cancer therapies subsequently used their frozen samples. This may question the economical validity of sperm cryopreservation programmes. However, in most contributions, the duration of follow-up was insufficient to draw robust information on the real rate of use. OBJECTIVES: To shed more light on the potential benefits of cryopreservation programmes. MATERIALS AND METHODS: Men who cryopreserved their semen in a public hospital for a diagnosis of cancer between 1986 and 2009 were retrospectively reviewed. The rate of use as well as the possible determinants was investigated. RESULTS: The median time of follow-up was 12 [IQR: 7-16] years. One hundred forty-four patients out of 1,524 (9.4%, 95%CI: 8.1%-11.0%) used their frozen samples of whom 64% were azoospermic. The rate of men achieving parenthood with frozen semen was 46%. Predictive factors of use were older age at the time of storage, lower sperm count at the time of storage and a diagnosis of testicular cancer. The impact of this latter factor was also supported by the lower frequency of azoospermia after cancer treatment in these patients. DISCUSSION: Cost-beneficial studies are warranted to assess and possibly improve the economical validity of sperm banking. CONCLUSION: The usage rate of frozen sperm in cancer patient is low, even extending the duration of follow-up.


Assuntos
Criopreservação , Preservação da Fertilidade/estatística & dados numéricos , Bancos de Esperma/estatística & dados numéricos , Espermatozoides , Adolescente , Adulto , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias , Estudos Retrospectivos , Análise do Sêmen , Adulto Jovem
11.
J Endocrinol Invest ; 44(5): 1091-1096, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33040303

RESUMO

PURPOSE: Sperm cryopreservation is fundamental in the management of patients undergoing gonadotoxic treatments. Concerns have risen in relation to SARS-CoV-2 and its potential for testicular involvement, since SARS-CoV-2-positive cryopreserved samples may have unknown effects on fertilization and embryo safety. This study therefore aimed to analyze the safety of sperm cryopreservation for cancer patients after the onset of the pandemic in Italy, through assessment of the risk of SARS-CoV-2 exposure and viral RNA testing of semen samples. METHODS: We recruited 10 cancer patients (mean age 30.5 ± 9.6 years) referred to our Sperm Bank during the Italian lockdown (from March 11th to May 4th 2020) who had not undergone a nasopharyngeal swab for SARS-CoV-2 testing. Patients were administered a questionnaire on their exposure to COVID-19, and semen samples were taken. Before cryopreservation, SARS-CoV-2 RNA was extracted from a 150 µl aliquot of seminal fluid in toto using QIAamp viral RNA kit (Qiagen) and amplified by a real time RT PCR system (RealStar SARS-CoV2 RT PCR, Altona Diagnostics) targeting the E and S genes. RESULTS: The questionnaire and medical interview revealed that all patients were asymptomatic and had had no previous contact with COVID-19 infected patients. All semen samples were negative for SARS-CoV-2 RNA. CONCLUSION: This preliminary assessment suggests that a thorough evaluation (especially in the setting of a multidisciplinary team) and molecular confirmation of the absence of SARS-CoV-2 in seminal fluid from asymptomatic cancer patients may assist in ensuring the safety of sperm cryopreservation.


Assuntos
COVID-19 , Criopreservação/estatística & dados numéricos , Pandemias , Preservação do Sêmen/estatística & dados numéricos , Adolescente , Adulto , COVID-19/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/complicações , Segurança do Paciente , RNA Viral/análise , Reação em Cadeia da Polimerase em Tempo Real , Cidade de Roma/epidemiologia , Bancos de Esperma , Adulto Jovem
12.
Zhonghua Nan Ke Xue ; 26(7): 616-619, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-33377717

RESUMO

OBJECTIVE: To analyze the efficiency of sperm donation by qualified donors and provide some experience for improving the success rate of sperm donation in human sperm banks. METHODS: This study included 440 qualified sperm donors in Chongqing Human Sperm Bank from April 2015 to June 2019. We analyzed the general information about the donors, the causes of failed sperm donation and the results of semen bacterial culture. RESULTS: Among the 440 qualified donors, 11 (2.50%) did not donate sperm, 28 (6.36%) were excluded because of frequent failures to donate, 397 (90.2%) completed all the procedures of sperm donation, and 4 (0.91%) failed to undergo HIV test six months after the last donation. The 397 donors that fulfilled the procedures donated sperm for 2 965 person-times, of which 2 159 (72.8%) were qualified and 806 (27.2%) unqualified for substandard semen quality (n = 684 ï¼»23.1%ï¼½), semen volume <2 ml (n = 33 ï¼»1.11%ï¼½), abnormal seminal liquefaction (n = 14 ï¼»0.47%ï¼½), or positive semen bacterial culture (n = 75 ï¼»2.53%ï¼½). CONCLUSIONS: Substandard semen quality is the main factor affecting the efficiency of sperm donation. The staff of the human sperm bank should pay adequate attention to the first reception of and communication with the donors, dispel their worries, enhance health care guidance, prevent pollution and improve the success rate of sperm donation.


Assuntos
Bancos de Esperma/estatística & dados numéricos , Obtenção de Tecidos e Órgãos , Humanos , Masculino , Análise do Sêmen , Espermatozoides , Doadores de Tecidos
13.
Environ Res ; 186: 109528, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32668535

RESUMO

Ambient air pollution has adverse effects on human health and reproduction. A number of studies have suggested a significant association between ambient air pollution and human fecundity, with most studies focusing on cross-sectional data from the general male population with single semen samples. We conducted a retrospective study in China using longitudinal analysis of repeated semen samples to investigate the association between environmental exposure parameters (e.g., PM2.5, PM10, SO2, NO2, CO, ground temperature, and relative humidity) and sperm quality parameters (e.g., semen volume, sperm concentration, forward motility concentration, and percentage of progressive rate) during different exposure windows (current day, 90-day preceding). Data from 686 males and 4841 semen samples collected between 2013 and 2018 at Sichuan Provincial Sperm Bank were included in a mixed-effects model analysis. The study population was young, healthy, and well-educated. The results indicate that 90-day average concentrations of PM2.5, PM10, and CO were negatively associated with sperm concentration and forward motility concentration, whereas 90-day average concentration of O3 was positively associated with forward motility concentration. Between-subject variability played the dominant role in overall model variance. Our results suggest that chronic exposure to particulate matter and CO may interfere with spermatogenesis.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , China , Estudos Transversais , Fertilidade , Humanos , Masculino , Material Particulado/análise , Estudos Retrospectivos , Bancos de Esperma , Espermatozoides
14.
Pediatr Blood Cancer ; 67(10): e28217, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32453503

RESUMO

BACKGROUND: Adolescent and young adult (AYA) males newly diagnosed with cancer are often faced with making quick decisions about whether to cryopreserve ("bank") sperm prior to treatment initiation. Given that parental influence is crucial among young patients, the present study examines the prevalence of and factors associated with parent recommendation to bank sperm. PROCEDURE: Parents of 13- to 21-year-old males newly diagnosed with cancer and at risk for infertility secondary to impending gonadotoxic treatment completed questionnaires typically within one week of treatment initiation. Medical and sociodemographic data, communication factors, and psychological factors were considered in a logistic regression model of parent report of parental recommendation to bank sperm (yes/no). RESULTS: Surveys from 138 parents (70.3% female) of 117 AYA males (mean age = 16.1 years, SD = 2.0) were analyzed. Over half of parents recommended banking to their sons (N = 82; 59.4%). Parents who received a provider recommendation to bank sperm (odds ratio [OR] = 18.44, 95% confidence interval [CI], 4.20-81.01, P < 0.001) or who believed in the benefits of banking (OR = 1.22, 95% CI, 1.02-1.47, P = 0.03) were significantly more likely to recommend sperm banking. CONCLUSIONS: Given parents' role in influencing sperm banking outcomes, provider recommendation and promotion of banking benefits may influence parents and empower initiation of these sensitive discussions with their sons. Utilization of this approach should yield beneficial outcomes regardless of the banking decision.


Assuntos
Preservação da Fertilidade/psicologia , Infertilidade Masculina/prevenção & controle , Neoplasias/terapia , Pais/psicologia , Aceitação pelo Paciente de Cuidados de Saúde , Preservação do Sêmen/psicologia , Bancos de Esperma/estatística & dados numéricos , Adolescente , Adulto , Comunicação , Estudos Transversais , Feminino , Seguimentos , Humanos , Infertilidade Masculina/psicologia , Masculino , Pessoa de Meia-Idade , Neoplasias/patologia , Neoplasias/psicologia , Relações Pais-Filho , Espermatozoides/química , Inquéritos e Questionários , Adulto Jovem
16.
J Reprod Infant Psychol ; 38(5): 474-484, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-31852263

RESUMO

Objective: This study aimed to explore which topics intended parents who opt for donor sperm treatment find relevant to discuss in psychosocial counselling. Background: The choice for donor sperm treatment has psychosocial implications for intended parents and therefore psychosocial counselling is advised as an integral part of DST. To date, little is known about which topics intended parents find relevant to discuss in psychosocial counselling. Methods: We conducted 25 semi-structured in-depth interviews between 2015 and 2017 with heterosexual men and women, lesbian women and single women who opted for donor sperm treatment and had a counselling session as part of their intake. They were recruited through three Dutch fertility centres, three network organisations and by snowball sampling. Results: Intended parents found it relevant to discuss the following seven topics in psychosocial counselling: the decision to opt for donor sperm treatment, choosing a sperm donor, coping with questions from family and friends, non-genetic parenthood, single motherhood, openness and disclosure, and future contact between the child and half-siblings. Conclusion: We recommend that counsellors take a more active role in bringing up the topics found in our study and that a clear distinction is made between counselling with the aim to screen intended parents and counselling with the aim to offer guidance.


Assuntos
Aconselhamento/métodos , Inseminação Artificial Heteróloga/psicologia , Pais/psicologia , Bancos de Esperma , Adulto , Tomada de Decisões , Revelação , Feminino , Homossexualidade Feminina/psicologia , Humanos , Entrevistas como Assunto , Masculino , Pessoa de Meia-Idade , Países Baixos , Relações Pais-Filho
17.
Support Care Cancer ; 28(8): 3915-3919, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31858247

RESUMO

PURPOSE: In developing countries, a higher percentage of patients develop cancer at a younger age. Cancer survival rates have significantly improved, highlighting the importance of survivorship programs that address late complications related to cancer itself or its treatment. The purpose of this study is to estimate the prevalence of fertility counseling and sperm banking and related factors among at-risk males newly diagnosed with cancer and planning to receive a potentially curative anticancer therapy. METHODS: Medical records and hospital database of young male patients with newly diagnosed cancers and planned to start chemotherapy were reviewed for fertility counseling and sperm cryopreservation. Additionally, a self-administered questionnaire was utilized. RESULTS: A total of 186 patients, mean age 32.9 (range: 18-53) years, were included. Non-Hodgkin's lymphoma 59 (31.7%), leukemia 48 (25.8%), and Hodgkin's lymphoma 26 (14.0%) were the most common tumors encountered. A total of 129 (75.0%) respondents received fertility counseling prior to their treatment, and this rate was higher among patients with early-stage disease (82.4% vs. 58.1%, p = 0.038). However, sperm banking was performed by 33.1% of the whole study group but was significantly higher among single patients (53.4% vs. 17.7%, p < 0.001), those who had no children (51.8% vs. 14.3%, p < 0.001), and among highly educated patients (47.6% vs. 17.1%, p = 0.001). Patients failed to do sperm banking because they were not informed about the risk of infertility (26.2%) or service availability (25.4%). Fear of treatment delay was a reason in 20.0%. CONCLUSIONS: Fertility counseling and sperm banking among cancer patients are not optimal. Many patients failed to do sperm banking because of avoidable reasons. Better communication and patients' education will probably improve the utilization of this vital service.


Assuntos
Aconselhamento/métodos , Preservação da Fertilidade/estatística & dados numéricos , Neoplasias/tratamento farmacológico , Bancos de Esperma/estatística & dados numéricos , Adolescente , Adulto , Comunicação , Criopreservação/métodos , Criopreservação/estatística & dados numéricos , Países em Desenvolvimento , Preservação da Fertilidade/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/psicologia , Preservação do Sêmen/métodos , Preservação do Sêmen/estatística & dados numéricos , Bancos de Esperma/métodos , Inquéritos e Questionários , Adulto Jovem
18.
Rev Int Androl ; 18(1): 27-34, 2020.
Artigo em Espanhol | MEDLINE | ID: mdl-30477959

RESUMO

Some treatments for any cancer therapy and hematological diseases may have gonadotoxic side effects that can result in infertility, and thus sperm cryopreservation is routinely offered to patients as the strategy to preserve their fertility. However, there are many cases where sperm banking cannot be applied, as is the case of pre-pubertal cancer patients and others unable to produce mature gametes at the moment of diagnosis. Regarding this, recent breakthroughs have gained public attention to the fertility preservation options that Regenerative Medicine can offer to these patients. In this review, we tried to compile and discuss the latest updates about all these strategies from a critical point of view.


Assuntos
Criopreservação , Preservação da Fertilidade/métodos , Bancos de Esperma , Células-Tronco , Testículo , Fatores Etários , Animais , Criança , Pré-Escolar , Humanos , Lactente , Infertilidade Masculina/etiologia , Masculino , Camundongos , Neoplasias/terapia , Medicina Regenerativa , Espermatogênese , Espermatozoides , Transplante de Células-Tronco/métodos
19.
Int J Clin Oncol ; 25(3): 495-500, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31452020

RESUMO

BACKGROUND: Testicular cancer is a rare type of cancer in males. Since the disease is seen in young men and long-term survival is ensured following a high treatment success rate, fertility in testicular cancer patients is much more important. Prior to commencement of cancer treatment, patients are given counselling with regard to infertility and sexual function, and sperm banking is commonly carried out. The aim of this study was to assess the fertility status prior to and following treatment of monitored testicular cancer patients whose treatment had been completed. METHODS: 110 patients diagnosed with and treated for testicular cancer at the Medical Oncology Clinic at Akdeniz University during the years 2000-2016 were evaluated for the study. The patients' disease and treatment information was obtained from their records. The patients' characteristics and fertility statuses were determined by means of interviews with the patients. RESULTS: The median age of the patients was 36 (20-73) and 39.1% of them (n = 43) were aged between 30 and 39. The average length of follow-up was 6.20 ± 3.36 (2-17) years. It was determined that 42.7% of the patients had banked sperm following diagnosis and that 74.5% of them had received counselling. Following treatment, 33 patients (30%) fathered children. The average time taken to father children after treatment was 3 years. CONCLUSION: In testicular cancer patients, fatherhood is achieved spontaneously or with the cryopreservation process. Counselling plays an important role at the time of diagnosis. It is essential that health professionals in oncology clinics give counselling about fertility in testicular cancer.


Assuntos
Preservação da Fertilidade , Preservação do Sêmen , Neoplasias Testiculares/terapia , Adulto , Idoso , Criopreservação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Gravidez , Projetos de Pesquisa , Fatores Socioeconômicos , Bancos de Esperma , Espermatozoides , Neoplasias Testiculares/patologia , Turquia
20.
Hum Reprod ; 34(11): 2208-2218, 2019 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-31711146

RESUMO

STUDY QUESTION: How do the demographic characteristics, motivations, experiences and expectations of unregulated sperm donors (men donating sperm online through a connection website) compare to sperm donors in the regulated sector (men donating through a registered UK sperm bank)? SUMMARY ANSWER: Online donors were more likely to be older, married and have children of their own than sperm bank donors, were more varied in their preferences and expectations of sperm donation, and had more concerns about being a sperm donor. WHAT IS KNOWN ALREADY: While studies have examined motivations and experiences of both regulated sperm bank, and unregulated online sperm donors, no study has directly compared these two groups of donors. STUDY DESIGN, SIZE, DURATION: An email was sent to the 576 men who were registered sperm donors at the London Sperm Bank, the UK's largest sperm bank regulated by the Human Fertilisation and Embryology Authority (HFEA), who had commenced donation between January 2010 and December 2016, and had consented to be contacted for research. The online survey, which contained multiple choice and open-ended questions, was completed by 168 men over a 7-week period. The responses were compared to those of sperm donors registered on Pride Angel, a large UK-based connection website for donors and recipients of sperm: our research team had already collected these data. In total, 5299 sperm donors were on Pride Angel at time of data capture and 400 men had completed a similar survey. The responses of 70 actual online sperm donors (i.e. those whose sperm had been used to conceive at least one child) were used for comparison with the sperm bank donors. PARTICIPANTS/MATERIALS, SETTING, METHODS: The survey obtained data on the sperm donors' demographic characteristics, motivations, experiences and expectations of sperm donation. Data from sperm bank donors were compared to online donors to examine differences between the two groups. The study compared online and clinic donors who had all been accepted as sperm donors: online donors who had been 'vetted' by recipients and sperm bank donors who had passed the rigorous screening criteria set by the clinic. MAIN RESULTS AND THE ROLE OF CHANCE: A response rate of 29% was obtained from the sperm bank donors. Online donors were significantly older than sperm bank donors (mean ± SD: 38.7 ± 8.4 versus 32.9 ± 6.8 years, respectively) and were more likely to have their own children (p < 0.001 for both characteristics). Both groups rated the motivation 'I want to help others' as very important. Online donors rated 'I don't want to have children myself', 'to have children/procreate' and 'to enable others to enjoy parenting as I have myself' as more important than sperm bank donors, whereas sperm bank donors rated financial payment as more important than online donors, as well as confirmation of own fertility. Most (93.9%) online donors had donated their sperm elsewhere, through other connection sites, fertility clinics, sperm banks or friends and family, compared to only 2.4% of sperm bank donors (p < 0.001). There was a significant difference in how donors viewed their relationship to the child, with online donors much less likely than sperm bank donors to see their relationship as a 'genetic relationship only'. Online donors had more concerns about being a donor (p < 0.001), for example, being concerned about 'legal uncertainty and child financial support' and 'future contact and uncertainty about relationship with donor-conceived child'. LIMITATIONS, REASONS FOR CAUTION: Findings may not be representative of all sperm donors as only one online connection site and one HFEA registered sperm bank were used for recruitment. WIDER IMPLICATIONS OF THE FINDINGS: Despite concern regarding shortages of sperm donors in licensed clinics and unease regarding the growing popularity of unregulated connection websites, this is the first study to directly compare online and sperm bank donors. It highlights the importance of considering ways to incorporate unregulated online sperm donors into the regulated sector. With many online donors well aware of the legal risks they undertake when donating in the unregulated online market, this would both increase the number of sperm donors available at clinics but also provide legal protection and support for donors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by the Wellcome Trust Grants 104 385/Z/14/Z and 097857/Z/11/Z. The authors have no conflicts of interest.


Assuntos
Internet , Motivação , Bancos de Esperma , Doadores de Tecidos/psicologia , Obtenção de Tecidos e Órgãos , Altruísmo , Humanos , Londres , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários
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