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1.
ACS Sens ; 8(1): 176-186, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36604942

RESUMO

Wearable sweat sensors, a product of the development of flexible electronics and microfluidic technologies, can continuously and noninvasively monitor abundant biomarkers in human sweat; however, sweat interferences, such as sebum, can reduce sensor reliability and accuracy. Herein, for the first time, the influence of sebum on the potentiometric response of an all-solid-state pH sensor was studied, and the obtained experimental results show that sebum mixed in sweat can decrease the potential response of the sensor and the slope of its calibration curve. A paper-based sandwich-structured pH sensor that can filter the sebum mixed in sweat was proposed based on commonly used oil-control sheets. Moreover, the hydrophilic properties, microstructure, and microfluidic performance of the sensor were investigated. The detection performance of the paper-based sandwich-structured pH sensor was comprehensively evaluated in terms of calibration in the presence of sebum and potentiometric response upon the addition of sebum. Furthermore, the anti-interference ability of the sensor was evaluated using different analytes under various deformation conditions. On-body trials were conducted to verify the performance, and their results showed that the proposed sensor can filter over 90% of the sebum in sweat, significantly enhancing sensor reliability and accuracy. Additionally, microfluidic channels could be simply fabricated using a scissor and paper, obviating the need for complex micromachining processes, such as photolithography and laser engraving. Overall, this work illustrates the influence of sebum on the detection performance of traditional potentiometric wearable sensors and paves the way for their development for real-world applications.


Assuntos
Suor , Dispositivos Eletrônicos Vestíveis , Humanos , Suor/química , Sebo , Reprodutibilidade dos Testes , Concentração de Íons de Hidrogênio
2.
Nat Commun ; 14(1): 69, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36604444

RESUMO

Sweat sensors play a significant role in personalized healthcare by dynamically monitoring biochemical markers to detect individual physiological status. The specific response to the target biomolecules usually depends on natural oxidase, but it is susceptible to external interference. In this work, we report tryptophan- and histidine-treated copper metal-organic frameworks (Cu-MOFs). This amino-functionalized copper-organic framework shows highly selective activity for ascorbate oxidation and can serve as an efficient ascorbate oxidase-mimicking material in sensitive sweat sensors. Experiments and calculation results elucidate that the introduced tryptophan/histidine fundamentally regulates the adsorption behaviors of biomolecules, enabling ascorbate to be selectively captured from complex sweat and further efficiently electrooxidized. This work provides not only a paradigm for specifically sweat sensing but also a significant understanding of natural oxidase-inspired MOF nanoenzymes for sensing technologies and beyond.


Assuntos
Oxirredutases , Suor , Suor/química , Cobre/química , Histidina , Triptofano
3.
Biosensors (Basel) ; 13(1)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36671938

RESUMO

Simultaneous detection of uric acid and glucose using a non-invasive approach can be a promising strategy for related diseases, e.g., diabetes, gout, kidney disease, and cardiovascular disease. In this study, we have proposed a dual-function wearable electrochemical sensor for uric acid and glucose detection in sweat. The sensor with a four-electrode system was prepared by printing the ink on a common rubber glove. CV and chronoamperometry were used to characterize the prepared sensor's electrochemical sensing performance. The sensors exhibited the linear range from 0 to 1.6 mM and 0 to 3.7 mM towards uric acid and glucose electrochemical sensing in phosphate-buffered solution, with the corresponding limit of detection of 3.58 µM and 9.10 µM obtained, respectively. Moreover, the sensors had shown their feasibility of real sample sensing in sweat. The linear detection range for uric acid (0 to 40 µM) and glucose (0 to 1.6 mM) in the sweat can well cover their concentration range in physiological conditions. The prepared dual-function wearable electrochemical sensor features easy preparation, fast detection, high sensitivity, high selectivity, and the practical application potential in uric acid and glucose sensing.


Assuntos
Técnicas Biossensoriais , Dispositivos Eletrônicos Vestíveis , Suor/química , Ácido Úrico/análise , Glucose/análise , Eletrodos , Técnicas Eletroquímicas
4.
Biosensors (Basel) ; 13(1)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36671962

RESUMO

The current physical health care system has gradually evolved into a form of virtual hospitals communicating with sensors, which can not only save time but can also diagnose a patient's physical condition in real time. Textile-based wearable sensors have recently been identified as detection platforms with high potential. They are developed for the real-time noninvasive detection of human physiological information to comprehensively analyze the health status of the human body. Sweat comprises various chemical compositions, which can be used as biomarkers to reflect the relevant information of the human physiology, thus providing references for health conditions. Combined together, textile-based sweat sensors are more flexible and comfortable than other conventional sensors, making them easily integrated into the wearable field. In this short review, the research progress of textile-based flexible sweat sensors was reviewed. Three mechanisms commonly used for textile-based sweat sensors were firstly contrasted with an introduction to their materials and preparation processes. The components of textile-based sweat sensors, which mainly consist of a sweat transportation channel and collector, a signal-selection unit, sensing elements and sensor integration and communication technologies, were reviewed. The applications of textile-based sweat sensors with different mechanisms were also presented. Finally, the existing problems and challenges of sweat sensors were summarized, which may contribute to promote their further development.


Assuntos
Suor , Dispositivos Eletrônicos Vestíveis , Humanos , Suor/química , Têxteis , Biomarcadores/análise
6.
ACS Appl Mater Interfaces ; 15(1): 469-478, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36577013

RESUMO

Wearable sweat sensors have been developed rapidly in recent years due to the great potential in health monitoring. Developing a convenient manufacturing process and a novel structure to realize timeliness and continuous monitoring of sweat is crucial for the practical application of sweat sensors. Herein, inspired by the striped grooves and granular structures of bamboo leaves, we realized an epidermal patch with biomimetic multilevel structural microfluidic channels for timeliness monitoring of sweat via 3D printing and femtosecond laser processing. The striped grooves and ridges are alternately arranged at the bottom of the microfluidic channels, and the surface of the ridges has rough granular structures. The striped grooves improve the capillary effect in the microchannels by dividing the microchannels, and the granular structures enhance the slip effect of sweat by increasing surface hydrophobicity. The experimental results show that compared with the conventional microfluidic channels, the water collecting rate of the biomimetic microchannels increased by about 60%, which is consistent with the theoretical analysis. The superior sweat-collecting efficiency in the epidermal patch with the biomimetic multistructure enables sensitive, continuous, and stable monitoring of sweat physiological signals. Besides, this work provides new design and manufacturing approaches for other microfluidic applications.


Assuntos
Técnicas Biossensoriais , Suor , Suor/química , Microfluídica , Técnicas Biossensoriais/métodos , Biomimética , Epiderme
7.
Biosens Bioelectron ; 223: 114994, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36577175

RESUMO

Herein, we introduce wearable potentiometric biosensors on screen-printed carbon electrodes (SPCEs) for on-body and on-site monitoring of urea in sweat. The biosensor architecture was judiciously designed to detect urea at different pHs and incorporate a pH sensor, thus containing polyaniline ink, urease bioink and a polyvinylchloride membrane. Urea detection could be performed in the wide range from 5 to 200 mM at pH 7.0, encompassing urea levels in human sweat. The biosensor response was fast (incubation time 5 min), with no interference from other substances in sweat. Reliable urea detection could be done in undiluted human sweat with a skin-worn flexible device using the pH correction strategy afforded by the pH sensor. The performance of the epidermal biosensor was not affected by severe bending strains. The feasibility of mass production was demonstrated by fabricating epidermal flexible biosensors using slot-die coating with a roll-to-roll technique.


Assuntos
Técnicas Biossensoriais , Dispositivos Eletrônicos Vestíveis , Humanos , Técnicas Biossensoriais/métodos , Suor/química , Ureia/análise , Potenciometria
8.
Sensors (Basel) ; 22(24)2022 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-36559954

RESUMO

A novel exercise modality combined with electrical muscle stimulation (EMS) has been reported to increase cardiovascular and metabolic responses, such as blood lactate concentration. We aimed to examine the effect of constant load pedaling exercise, combined with EMS, by non-invasively and continuously measuring sweat lactate levels. A total of 22 healthy young men (20.7 ± 0.8 years) performed a constant load pedaling exercise for 20 min at 125% of the pre-measured ventilatory work threshold with (EMS condition) and without (control condition) EMS stimulation. Blood lactate concentration was measured by blood samples obtained from the earlobe every minute. Sweat lactate was monitored in real time using a sensor placed on the forearm. The sweat lactate threshold (sLT) was defined as the point of increase in sweat lactate. sLT occurred significantly earlier in the EMS condition than in the control condition. In the single regression analysis, the difference in sLT between the two conditions, as the independent variable, was a significant predictor of the difference in blood lactate concentrations at the end of the exercise (p < 0.05, r = -0.52). Sweat lactate measurement may be a noninvasive and simple alternative to blood lactate measurement to determine the effectiveness of exercise combined with EMS.


Assuntos
Exercício Físico , Suor , Masculino , Humanos , Exercício Físico/fisiologia , Sudorese , Ácido Láctico , Músculo Esquelético/fisiologia
9.
Rev Prat ; 72(8): 819-823, 2022 Oct.
Artigo em Francês | MEDLINE | ID: mdl-36511977

RESUMO

THERAPEUTIC ADVANCES IN CYSTIC FIBROSIS: FROM GENETICS TO TREATMENT PERSONALIZED. Cystic fibrosis is a severe monogenic disease that affects around 7 300 patients in France. Mutations (> 2 000) in CFTR, the gene encoding for an epithelial ion channel that normally transports chloride and bicarbonate ions, lead to mucus dehydration and impaired bronchial clearance and pancreatic functions. Systematic neonatal screening in France has allowed early diagnosis since 2002. Although highly restrictive, supportive treatments including daily chest physiotherapy, inhaled aerosol therapy, frequent antibiotic courses, nutritional and pancreatic extracts have improved the prognosis. Median age at death is now beyond 30 years of age. Ivacaftor was the first CFTR potentiator found to both reduce sweat chloride concentrations and improve pulmonary function. Then, combinations of a potentiator and various correctors such as lumacaftor + ivacaftor or tezacaftor + ivacaftor have been tested. Finally, the triple association ivacaftor + tezacaftor + elexacaftor was recently shown to normalize sweat chloride concentration, significantly improve pulmonary function testing, reduce the need for antibiotic treatments, and ultimately improve the quality of life in patients with at least oneF508del mutation (83% of patients in France).


AVANCÉES THÉRAPEUTIQUES DANS LA MUCOVISCIDOSE : DE LA GÉNÉTIQUE AU TRAITEMENT PERSONNALISÉ. La mucoviscidose est une maladie monogénique affectant environ 7 300 patients en France. Plus de 2 000 mutations dans le gène CFTR codant pour la protéine CFTR, canal épithélial qui transporte les ions chlorure et bicarbonate, conduisent à la production d'un mucus déshydraté et visqueux qui altère les fonctions respiratoire et pancréatique. Le dépistage néonatal est systématique en France depuis 2002. Bien que très contraignants, les traitements symptomatiques ont permis de porter l'âge médian au décès au-delà de 30 ans. L'ivacaftor, un potentiateur de la fonction CFTR, a été le premier médicament à faire la preuve de son efficacité, avec une diminution nette des symptômes. Puis ont été testées les combinaisons d'un potentiateur et de correcteurs de la protéine CFTR : lumacaftor-ivacaftor ou tezacaftor-ivacaftor. Enfin, la triple association ivacaftor-tezacaftor- elexacaftor a permis de normaliser la teneur en chlorure sudoral (biomarqueur de la fonction protéique CFTR au niveau des glandes sudorales), d'améliorer durablement la fonction respiratoire (VEMS), de diminuer les exacerbations pulmonaires et la consommation d'antibiotiques chez les patients homozygotes ou hétérozygotes composites pour la mutation F508del (83 % des patients en France).


Assuntos
Fibrose Cística , Humanos , Aminofenóis/uso terapêutico , Antibacterianos/uso terapêutico , Agonistas dos Canais de Cloreto/uso terapêutico , Cloretos/metabolismo , Fibrose Cística/tratamento farmacológico , Fibrose Cística/genética , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Combinação de Medicamentos , Mutação , Qualidade de Vida , Suor/metabolismo
10.
ACS Appl Mater Interfaces ; 14(50): 56074-56086, 2022 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-36508579

RESUMO

Most electronic skins (e-skins) show unique performance or possess sensory functions. The raw materials used for their preparation are potentially toxic or harmful, and there may be problems such as poor compatibility between the conductive fillers and polymers. In this paper, a silver-loaded nanocomposite film (PVA/CMS/vanillin/nanoAg) was prepared by the in situ reduction method in a greener route. The mechanical properties of this nanocomposite film had improved with a tensile strength of 30.95 MPa, an elongation at break of 101.9%, and a Young's modulus of 10.62 MPa. In the composite matrix, a cross-linked network was constructed based on the coordination and hydrogen bonds, which was conducive to the stability of the reduced AgNPs and AgNWs. When applied as an e-skin in humidity/sweat sensors and wearable electronics, the nanocomposite film responds to humidity within 60 s and records the electric signals of human joint movements and skin sweating with a response range of 0-140% to strain at 93% RH. This kind of e-skin has excellent antibacterial and antioxidant activities and shows an outstanding ultraviolet-proof performance, which provides a greener promising reference route for the design of wearable e-skins to monitor the health and movements of humans.


Assuntos
Sudorese , Dispositivos Eletrônicos Vestíveis , Humanos , Antioxidantes/farmacologia , Antioxidantes/análise , Suor/química , Umidade , Eletrônica , Antibacterianos/farmacologia , Antibacterianos/análise
11.
ACS Sens ; 7(12): 3973-3981, 2022 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-36512725

RESUMO

ß-Hydroxybutyrate (HB) is one of the main physiological ketone bodies that play key roles in human health and wellness. Besides their important role in diabetes ketoacidosis, ketone bodies are currently receiving tremendous attention for personal nutrition in connection to the growing popularity of oral ketone supplements. Accordingly, there are urgent needs for developing a rapid, simple, and low-cost device for frequent onsite measurements of ß-hydroxybutyrate (HB), one of the main physiological ketone bodies. However, real-time profiling of dynamically changing HB concentrations is challenging and still limited to laboratory settings or to painful and invasive measurements (e.g., a commercial blood ketone meter). Herein, we address the critical need for pain-free frequent HB measurements in decentralized settings and report on a reliable noninvasive, simple, and rapid touch-based sweat HB testing and on its ability to track dynamic HB changes in secreted fingertip sweat, following the intake of commercial ketone supplements. The new touch-based HB detection method relies on an instantaneous collection of the fingertip sweat at rest on a porous poly(vinyl alcohol) (PVA) hydrogel that transports the sweat to a biocatalytic layer, composed of the ß-hydroxybutyrate dehydrogenase (HBD) enzyme and its nicotinamide adenine dinucleotide (NAD+) cofactor, covering the modified screen-printed carbon working electrode. As a result, the sweat HB can be measured rapidly by the mediated oxidation reaction of the nicotinamide adenine dinucleotide (NADH) product. A personalized HB dose-response relationship is demonstrated within a group of healthy human subjects taking commercial ketone supplements, along with a correlation between the sweat and capillary blood HB levels. Furthermore, a dual disposable biosensing device, consisting of neighboring ketone and glucose enzyme electrodes on a single-strip substrate, has been developed toward the simultaneous touch-based detection of dynamically changing sweat HB and glucose levels, following the intake of ketone and glucose drinks.


Assuntos
Glucose , Corpos Cetônicos , Humanos , Corpos Cetônicos/análise , Glucose/análise , Ácido 3-Hidroxibutírico , Tato , NAD , Autoteste , Suor/química , Cetonas
12.
Anal Chem ; 94(51): 18000-18008, 2022 12 27.
Artigo em Inglês | MEDLINE | ID: mdl-36524711

RESUMO

Herein, we developed a flexible, low-cost non-enzymatic sweat sensing chip for in situ acquisition of bioinformation in sweat of individuals under exercise conditions to advance personal health monitoring and medication management for patients with Parkinson's disease. This low-cost, flexible, wearable sweat sensor consists of a printed screen electrode modified with g-C3N4 material and an external MSME element. The doping strategy and surface activation strategy of the g-C3N4-based exhibited efficient glucose oxidase-like activity and electrochemical activity when testing l-dopa and glucose in sweat. The optimized signal was transmitted to a smartphone for processing 12 individuals with simulated dosing, enabling continuous monitoring of l-dopa metabolism in sweat and management of dosing. The generalization ability and robustness of models constructed by methods such as multiple linear regression, artificial neural networks, and convolutional neural networks were compared cross-sectionally. Deep learning models based on artificial neural networks help develop a user-personalized medication administration reminder system, which provides a promising paradigm for reliable medication supervision for Parkinson's patients in the Internet of Things era.


Assuntos
Técnicas Biossensoriais , Doença de Parkinson , Dispositivos Eletrônicos Vestíveis , Humanos , Levodopa/uso terapêutico , Doença de Parkinson/tratamento farmacológico , Suor , Glucose , Técnicas Biossensoriais/métodos
13.
Biosensors (Basel) ; 12(12)2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36551064

RESUMO

Wearable devices are being developed faster and applied more widely. Wearables have been used to monitor movement-related physiological indices, including heartbeat, movement, and other exercise metrics, for health purposes. People are also paying more attention to mental health issues, such as stress management. Wearable devices can be used to monitor emotional status and provide preliminary diagnoses and guided training functions. The nervous system responds to stress, which directly affects eye movements and sweat secretion. Therefore, the changes in brain potential, eye potential, and cortisol content in sweat could be used to interpret emotional changes, fatigue levels, and physiological and psychological stress. To better assess users, stress-sensing devices can be integrated with applications to improve cognitive function, attention, sports performance, learning ability, and stress release. These application-related wearables can be used in medical diagnosis and treatment, such as for attention-deficit hyperactivity disorder (ADHD), traumatic stress syndrome, and insomnia, thus facilitating precision medicine. However, many factors contribute to data errors and incorrect assessments, including the various wearable devices, sensor types, data reception methods, data processing accuracy and algorithms, application reliability and validity, and actual user actions. Therefore, in the future, medical platforms for wearable devices and applications should be developed, and product implementations should be evaluated clinically to confirm product accuracy and perform reliable research.


Assuntos
Desempenho Atlético , Técnicas Biossensoriais , Dispositivos Eletrônicos Vestíveis , Humanos , Reprodutibilidade dos Testes , Suor , Monitorização Fisiológica/métodos
14.
Biosensors (Basel) ; 12(12)2022 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-36551123

RESUMO

In this work, a 3D printed sensor modified with a water-stable complex of Fe(III) basic benzoate is presented for the voltammetric detection of glucose (GLU) in acidic epidermal skin conditions. The GLU sensor was produced by the drop-casting of Fe(III)-cluster ethanolic mixture on the surface of a 3D printed electrode fabricated by a carbon black loaded polylactic acid filament. The oxidation of GLU was electrocatalyzed by Fe(III), which was electrochemically generated in-situ by the Fe(III)-cluster precursor. The GLU determination was carried out by differential pulse voltammetry without the interference from common electroactive metabolites presented in sweat (such as urea, uric acid, and lactic acid), offering a limit of detection of 4.3 µmol L-1. The exceptional electrochemical performance of [Fe3O(PhCO2)6(H2O)3]∙PhCO2 combined with 3D printing technology forms an innovative and low-cost enzyme-free sensor suitable for noninvasive applications, opening the way for integrated 3D printed wearable biodevices.


Assuntos
Compostos Férricos , Glucose , Suor , Eletrodos , Impressão Tridimensional , Técnicas Eletroquímicas
15.
Anal Chem ; 94(48): 16847-16854, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36383471

RESUMO

Several groups have recently explored the idea of developing electrochemical paper-based wearable devices, specifically targeting metabolites in sweat. While these sensors have the potential to provide a breadth of analytical information, there are several key challenges to address before these sensors can be widely adopted for clinical interventions. Toward this goal, we describe the development of a paper-based electrochemical sensor for the detection of Staphylococcus aureus. Enabling the application, this report describes the use of paper-derived carbon electrodes, which were modified with a thin layer of sputtered gold (that minimizes lateral resistivity and significantly improves the electron transfer process) and with chitosan (used as a binder, to offer flexibility). The resulting material was laser-patterned and applied for the development of an electrochemical biosensor controlled (via a wireless connection) by a custom-built, portable potentiostat. As no interference was observed when exposed to other bacteria or common metabolites, this wearable system (paper-derived electrodes + potentiostat) has the potential to detect the presence of S. aureus in the skin, a commonly misdiagnosed and mistreated infection.


Assuntos
Técnicas Biossensoriais , Staphylococcus aureus , Técnicas Eletroquímicas/métodos , Técnicas Biossensoriais/métodos , Eletrodos , Suor
16.
Nutrients ; 14(21)2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36364948

RESUMO

Physiological and biological markers in different body fluids are used to measure the body's physiological or pathological status. In the field of sports and exercise medicine, the use of these markers has recently become more popular for monitoring an athlete's training response and assessing the immediate or long-term effects of exercise. Although the effect of exercise on different physiological markers using various body fluids is well substantiated, no article has undertaken a review across multiple body fluids such as blood, saliva, urine and sweat. This narrative review aims to assess various physiological markers in blood, urine and saliva, at rest and after exercise and examines physiological marker levels obtained across similar studies, with a focus on the population and study methodology used. Literature searches were conducted using PRISMA guidelines for keywords such as exercise, physical activity, serum, sweat, urine, and biomarkers, resulting in an analysis of 15 studies for this review paper. When comparing the effects of exercise on physiological markers across different body fluids (blood, urine, and saliva), the changes detected were generally in the same direction. However, the extent of the change varied, potentially as a result of the type and duration of exercise, the sample population and subject numbers, fitness levels, and/or dietary intake. In addition, none of the studies used solely female participants; instead, including males only or both male and female subjects together. The results of some physiological markers are sex-dependent. Therefore, to better understand how the levels of these biomarkers change in relation to exercise and performance, the sex of the participants should also be taken into consideration.


Assuntos
Líquidos Corporais , Esportes , Humanos , Masculino , Feminino , Exercício Físico/fisiologia , Esportes/fisiologia , Suor/química , Biomarcadores
17.
Sensors (Basel) ; 22(21)2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36365929

RESUMO

Wearable sensors for sweat biomarkers can provide facile analyte capability and monitoring for several diseases. In this work, a green wearable sensor for sweat absorption and chloride sensing is presented. In order to produce a sustainable device, polylactic acid (PLA) was used for both the substrate and the sweat absorption pad fabrication. The sensor material for chloride detection consisted of silver-based reference, working, and counter electrodes obtained from upcycled compact discs. The PLA substrates were prepared by thermal bonding of PLA sheets obtained via a flat die extruder, prototyped in single functional layers via CO2 laser cutting, and bonded via hot-press. The effect of cold plasma treatment on the transparency and bonding strength of PLA sheets was investigated. The PLA membrane, to act as a sweat absorption pad, was directly deposited onto the membrane holder layer by means of an electrolyte-assisted electrospinning technique. The membrane adhesion capacity was investigated by indentation tests in both dry and wet modes. The integrated device made of PLA and silver-based electrodes was used to quantify chloride ions. The calibration tests revealed that the proposed sensor platform could quantify chloride ions in a sensitive and reproducible way. The chloride ions were also quantified in a real sweat sample collected from a healthy volunteer. Therefore, we demonstrated the feasibility of a green and integrated sweat sensor that can be applied directly on human skin to quantify chloride ions.


Assuntos
Técnicas Biossensoriais , Dispositivos Eletrônicos Vestíveis , Humanos , Suor , Cloretos , Prata , Poliésteres , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos
18.
Nat Commun ; 13(1): 6705, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36344563

RESUMO

Sensory neurons generate spike patterns upon receiving external stimuli and encode key information to the spike patterns, enabling energy-efficient external information processing. Herein, we report an epifluidic electronic patch with spiking sweat clearance using a sensor containing a vertical sweat-collecting channel for event-driven, energy-efficient, long-term wireless monitoring of epidermal perspiration dynamics. Our sweat sensor contains nanomesh electrodes on its inner wall of the channel and unique sweat-clearing structures. During perspiration, repeated filling and abrupt emptying of the vertical sweat-collecting channel generate electrical spike patterns with the sweat rate and ionic conductivity proportional to the spike frequency and amplitude over a wide dynamic range and long time (> 8 h). With such 'spiking' sweat clearance and corresponding electronic spike patterns, the epifluidic wireless patch successfully decodes epidermal perspiration dynamics in an event-driven manner at different skin locations during exercise, consuming less than 0.6% of the energy required for continuous data transmission. Our patch could integrate various on-skin sensors and emerging edge computing technologies for energy-efficient, intelligent digital healthcare.


Assuntos
Técnicas Biossensoriais , Suor , Suor/química , Monitorização Fisiológica , Eletrodos , Íons/análise , Eletrônica
19.
Nat Methods ; 19(11): 1323, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36329274

Assuntos
Suor , Sudorese , Humanos , Atletas
20.
PLoS One ; 17(11): e0276844, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36322597

RESUMO

Home cage aggression causes poor welfare in male laboratory mice and reduces data quality. One of the few proven strategies to reduce aggression involves preserving used nesting material at cage change. Volatile organic compounds from the nesting material and several body fluids not only correlate with less home cage aggression, but with more affiliative allo-grooming behavior. To date, these compounds have not been tested for a direct influence on male mouse social behavior. This study aimed to determine if 4 previously identified volatile compounds impact home cage interactions. A factorial design was used with cages equally split between C57BL/6N and SJL male mice (N = 40). Treatments were randomly assigned across cages and administered by spraying one compound solution on each cage's nesting material. Treatments were refreshed after day 3 and during cage change on day 7. Home cage social behavior was observed throughout the study week and immediately after cage change. Several hours after cage change, feces were collected from individual mice to measure corticosterone metabolites as an index of social stress. Wound severity was also assessed after euthanasia. Measures were analyzed with mixed models. Compound treatments did not impact most study measures. For behavior, SJL mice performed more aggression and submission, and C57BL/6N mice performed more allo-grooming. Wound severity was highest in the posterior region of both strains, and the middle back region of C57BL/6N mice. Posterior wounding also increased with more observed aggression. Corticosterone metabolites were higher in C57BL/6N mice and in mice treated with 3,4-dimethyl-1,2-cyclopentanedione with more wounding. These data confirm previous strain patterns in social behavior and further validates wound assessment as a measure of escalated aggression. The lack of observed treatment effects could be due to limitations in the compound administration procedure and/or the previous correlation study, which is further discussed.


Assuntos
Líquidos Corporais , Corticosterona , Animais , Masculino , Camundongos , Agressão , Comportamento Animal , Abrigo para Animais , Camundongos Endogâmicos C57BL , Comportamento de Nidação , Comportamento Social , Suor
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