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1.
Food Chem ; 370: 131055, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34536782

RESUMO

Sulfonylureas (SUs) are a series of anti-diabetic drugs widely used for type 2 diabetes mellitus for clinic treat. However, it is often illegally adulterated in multi-herbal tea to improve the claimed anti-diabetic activity in recent years. In this study, a novel hapten was rationally designed, and a broad-specific monoclonal antibody (anti-SUs mAb) recognizing nine SUs was developed. This mAb was used to develop a colloidal gold lateral flow immunochromatographic assay (CG-LFIA). The anti-SUs mAb demonstrated half inhibitory concentration (IC50) ranged from 0.15 ng/mL to 3.25 ng/ mL for nine SUs by ELISA. The cut-off value of developed CG-LFIA for nine SUs was from 3 to 100 ng/ mL for the spiked samples. LC-MS/MS confirmed the reliability of the new CG-LFIA. The results indicated that the proposed CG-LFIA could be an ideal method in on-site screening surveillance assay for SUs illegally adulterated in multi-herbal tea products.


Assuntos
Diabetes Mellitus Tipo 2 , Chás de Ervas , Cromatografia de Afinidade , Cromatografia Líquida , Coloide de Ouro , Humanos , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem
2.
Analyst ; 146(22): 6874-6882, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34633393

RESUMO

As the use of non-steroidal anti-inflammatory drugs (NSAIDS) increases, their side effects have also attracted attention. Ibuprofen is one of the most widely-used NSAIDs. In this study, we screened the highly-sensitive and specific antibody 6E10, with an IC50 of 1.92 ng mL-1, and a linear range of 0.53-6.97 ng mL-1. In this study, we developed a rapid lateral flow immunochromatographic assay (ICA) strip method to detect ibuprofen in water or herbal tea. The cut-off limit of the strip is 10 ng mL-1 in water, and concentrations as low as 1 ng mL-1 can be detected in herbal tea samples, with the results obtained by the naked eye within 6 min. All the data were confirmed by high performance liquid chromatography-quadrupole time of flight-mass spectrometry (HPLC-QTOF-MS). This lateral-flow ICA strip is thus a rapid tool for on-site detection and screening of ibuprofen in water and herbal tea.


Assuntos
Ibuprofeno , Chás de Ervas , Cromatografia de Afinidade , Limite de Detecção , Água
3.
Nutrients ; 13(7)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201882

RESUMO

Tea is one of the most popular and widely consumed beverages worldwide, and possesses numerous potential health benefits. Herbal teas are well-known to contain an abundance of polyphenol antioxidants and other ingredients, thereby implicating protection and treatment against various ailments, and maintaining overall health in humans, although their mechanisms of action have not yet been fully identified. Autophagy is a conserved mechanism present in organisms that maintains basal cellular homeostasis and is essential in mediating the pathogenesis of several diseases, including cancer, type II diabetes, obesity, and Alzheimer's disease. The increasing prevalence of these diseases, which could be attributed to the imbalance in the level of autophagy, presents a considerable challenge in the healthcare industry. Natural medicine stands as an effective, safe, and economical alternative in balancing autophagy and maintaining homeostasis. Tea is a part of the diet for many people, and it could mediate autophagy as well. Here, we aim to provide an updated overview of popular herbal teas' health-promoting and disease healing properties and in-depth information on their relation to autophagy and its related signaling molecules. The present review sheds more light on the significance of herbal teas in regulating autophagy, thereby improving overall health.


Assuntos
Autofagia , Células/metabolismo , Saúde , Homeostase , Chás de Ervas , Animais , Humanos
4.
ACS Appl Mater Interfaces ; 13(28): 33449-33463, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34240595

RESUMO

Selective detection of active ingredients in complex samples has always been a crucial challenge because there are many disturbing compounds, especially structural analogues that interfere with the detection. In this work, a fluorescent covalent organic framework (named COF-TD), which can be used for the selective fluorescence detection and enrichment of myricetin from complex samples, was reported for the first time. The highly crystalline COF-TD with bright blue fluorescence was formed through a solution polymerization method by the condensation reaction between 4,4',4″-(1,3,5-triazine-2,4,6-triyl)trianiline and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde. Due to spatial size selectivity, multisites hydrogen bonding, and π-π interaction, myricetin can quench the fluorescence of COF-TD with an inner filter effect (IFE) and static quenching mechanisms as well as can be enriched on COF-TD. Myricetin can observably eliminate the interference of other compounds and selectively quench the fluorescence of COF-TD with a limit of detection (LOD) of 0.30 µg·mL-1. The high adsorption ability of COF-TD (Q = 124.6 mg·g-1) to myricetin was also obtained. Finally, a sensing platform based on COF-TD for myricetin was successfully developed and applied for the detection of myricetin from vine teas. In addition, COF-TD also showed good water sensing ability and could be used effectively to detect water content in organic solvent (1-18% water in acetone, 0.5-5% water in acetonitrile, 1-4.5% water in ethyl acetate, v/v). To the best of our knowledge, this is the first report where COF-TD was used to detect water in a relatively wide concentration range. In all, this work provided dual-functional fluorescent COFs with the properties of an adsorbent, opening up new methodologies for the simple, selective, and enrichment detection method for myricetin.


Assuntos
Flavonoides/análise , Corantes Fluorescentes/química , Estruturas Metalorgânicas/química , Água/análise , Adsorção , Ampelopsis/química , Flavonoides/química , Corantes Fluorescentes/síntese química , Limite de Detecção , Estruturas Metalorgânicas/síntese química , Espectrometria de Fluorescência/métodos , Chás de Ervas/análise
5.
Sci Rep ; 11(1): 14115, 2021 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-34239000

RESUMO

Excessive ingestion of fluorides might adversely affect the health of humans. Hence, this study aimed to investigate the concentrations of infusible fluoride in five different types of tea and herbal products; additionally, the probabilistic health risks associated with the ingestion of fluoride in drinking tea and herbal products were estimated. The highest and lowest concentrations of infusible fluoride were detected in black and white tea, respectively. On average, the highest amount of infusible fluoride was extracted following a short brewing time of 5 min in the case of black tea (2.54 mg/L), herbal tea (0.40 mg/L), and white tea (0.21 mg/L). The level of infusible fluoride during brewing was inversely associated with the leaf size of the tea and herbal products. Furthermore, the type of water used influenced the release of infusible fluoride; purified water yielded lower amounts of infused fluoride. The findings of the probabilistic health risk assessment indicated that the consumption of black tea can increase the fluoride intake leading to chronic exposure. Thus, the health risk posed by fluoride intake from drinking tea needs to be evaluated in more details in the future. Appropriate measures for health risk mitigation need to be implemented to minimize the total body burden of fluorides in humans.


Assuntos
Fluoretos/análise , Probabilidade , Medição de Risco , Chá/química , Chás de Ervas/análise , Adolescente , Adulto , Criança , Humanos , Folhas de Planta/anatomia & histologia , Fatores de Tempo , Incerteza , Água
6.
Jpn J Clin Oncol ; 51(9): 1471-1474, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34215870

RESUMO

The standard first-line treatment for patients with human epidermal growth factor 2-positive metastatic breast cancer is a combination therapy of trastuzumab, pertuzumab and docetaxel, and the standard second-line treatment is trastuzumab emtansine. However, it may be difficult for the elderly to maintain sufficient intensity of treatment due to severe adverse events of trastuzumab, pertuzumab and docetaxel. The aim of this trial is to confirm the non-inferiority of trastuzumab emtansine over trastuzumab, pertuzumab and docetaxel in terms of overall survival in elderly (65-year-old or more) patients with human epidermal growth factor 2-positive metastatic breast cancer. If improved overall survival and fewer toxicities are observed, trastuzumab emtansine may be a feasible new standard first-line treatment for elderly patients with human epidermal growth factor 2-positive metastatic breast cancer. A planned total 330 patients will be enrolled from 45 institutions over 6.5 years. This trial has been registered in the UMIN Clinical Trials Registry as UMIN000030783 [http://www.umin.ac.jp/ctr/index.htm].


Assuntos
Neoplasias da Mama , Chás de Ervas , Ado-Trastuzumab Emtansina , Idoso , Anticorpos Monoclonais Humanizados , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Neoplasias da Mama/tratamento farmacológico , Docetaxel/uso terapêutico , Feminino , Humanos , Japão , Oncologia , Receptor ErbB-2 , Trastuzumab/efeitos adversos
7.
Fitoterapia ; 152: 104913, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33932529

RESUMO

Vine tea has been used as a traditionally functional herbal tea in China for centuries, which exhibits paramount potential for chronic metabolic diseases. Herein, the inhibitory potential of vine tea toward human catechol-O-methyltransferase (hCOMT) was investigated. A practical bioactivity-guided fractionation combined with chemical profiling strategy was developed to identify the naturally occurring hCOMT inhibitors. Five flavonoids in vine tea displayed moderate to strong inhibition on hCOMT with IC50 values ranging from 0.96 µM to 42.47 µM, in which myricetin was the critically potent constituent against hCOMT. Inhibition kinetics assays and molecular docking simulations showed that myricetin could bind to the active site of COMT and inhibited COMT-catalyzed 3-BTD methylation in a mixed manner. Collectively, our findings not only suggested that the strong hCOMT inhibition of vine tea has guiding significance in the drug exposure of catechol drugs, but also identified a promising lead compound for developing more efficacious hCOMT inhibitors.


Assuntos
Inibidores de Catecol O-Metiltransferase/farmacologia , Flavonoides/farmacologia , Chás de Ervas , Inibidores de Catecol O-Metiltransferase/isolamento & purificação , Flavonoides/isolamento & purificação , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
8.
FEMS Microbiol Lett ; 368(9)2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-33970244

RESUMO

Stachys pilifera Benth is an endemic species of Stachys family found in Iran with a wide application as an herbal tea. The objective of this research was to evaluate the antimicrobial, antioxidant and cytotoxic activity of the essential oil from the aerial parts of S. pilifera. Essential oil (EO) composition analysis showed that cis-Chrysanthenyl acetate (24.9%), viridiflorol (18.3%), trans-Caryophyllene (9.8%), caryophyllene oxide (4.6%), α-terpineol (3.3%) and linalool (3.1%) were the most abundant components. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EO showed a higher antimicrobial activity against Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) than Gram-negative organisms (Escherichia coli, Shigella sonnei, Pseudomonas aeruginosa and Salmonella enterica subsp. Enterica). The antioxidant activity of EO was studied using DPPH, FRAP and ß-carotene/linoleic acid assays. IC50 for the DPPH, FRAP and ß-carotene/linoleic acid tests were 23.2, 28.7 and 16.1 µg/mL, respectively, that it was higher than the results for BHT (P ≤ 0.05). The cytotoxic activity of the EO was evaluated using HT29 and HUVEC cells and it was observed that by increasing in EO concentration from 0.026 to 19.4 ug/mL, the viability of the cells for HT29 and HUVEC reduced to 6.8 and 7.1%, respectively. The results from this study suggest the possibility to use the essential oils from S. pilifera Benth as a natural preservative in processed or packaged food due to its high antibacterial, antioxidant and cytotoxic activities.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Óleos Voláteis/análise , Óleos Voláteis/farmacologia , Folhas de Planta/química , Stachys/química , Antibacterianos/química , Antioxidantes/química , Bactérias/classificação , Sobrevivência Celular/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Células HT29 , Humanos , Testes de Sensibilidade Microbiana , Chás de Ervas
9.
Food Chem ; 354: 129497, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33752112

RESUMO

Aflatoxin B1 is the potential chemical contaminant of most concern during the production and storage of fermented tea. In this work, a simple, fast, sensitive, accurate, and inexpensive method has been developed and validated for the simultaneous detection of four aflatoxins in fermented tea based on a modified sample pretreatment method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Aflatoxins were extracted using acetonitrile and purified using mixed fillers (carboxyl multiwalled carbon nanotubes, hydrophilic-lipophilic balance, silica gel). Under optimum LC-MS conditions, the limits of quantification (LOQs) were 0.02-0.5 µg·kg-1. Recoveries from aflatoxins-fortified tea samples (1-12 µg·kg-1) were in the range of 78.94-105.23% with relative standard deviations (RSDs) less than 18.20%. The proposed method was applied successfully to determine aflatoxin levels in fermented tea samples.


Assuntos
Aflatoxinas/análise , Cromatografia Líquida de Alta Pressão/métodos , Chás de Ervas/análise , Aflatoxinas/isolamento & purificação , Interações Hidrofóbicas e Hidrofílicas , Limite de Detecção , Nanotubos de Carbono/química , Reprodutibilidade dos Testes , Sílica Gel/química , Espectrometria de Massas em Tandem
10.
J Food Sci ; 86(4): 1296-1305, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33733483

RESUMO

At present, screening of active ingredients from natural products for pharmacological and clinical research is mostly time-consuming and costly. In this study, a molecular network (MN) guided high performance liquid chromatography-ultraviolet-fluorescence detector (HPLC-UV-FLD) method was carried out to profile the global antioxidant activity compounds, including the trace amount ingredients in Camellia nitidissima Chi (CNC). Firstly, HPLC-UV-FLD postcolumn derivatization system was utilized to screen the antioxidants. Then the MN of CNC was established via mass spectrometry (MS) data for getting the connection between ingredient structures. As a result, HPLC-UV-FLD indicated three antioxidant ingredients: gallic acid (126.3 mg/g), catechin (564.8 mg/g), and salicylic acid (24.3 mg/g). Combined with the MN, the actives' precise location and connection relationship were clarified based on the structural similarities. A new antioxidant ingredient, okicamelliaside, was suggested and evaluated at free radical scavenging and enzymatic protection. The novel method of activity and structural correlation analysis based on MN could provide a useful guide for screening trace active ingredients in natural products. PRACTICAL APPLICATION: Three main ingredients were screened out from Camellia nitidissima Chi by HPLC-UV-FLD postcolumn derivatization system. Integrated molecular network and HPLC-UV-FLD analysis, a new type of antioxidant okicamelliaside was selected. The novel method of activity and structural correlation analysis based on molecular network could provide a useful guide for screening trace active ingredients in natural products.


Assuntos
Antioxidantes/análise , Camellia/química , Cromatografia Líquida de Alta Pressão/métodos , Chás de Ervas/análise , Catequina/análise , Fluorescência , Ácido Gálico/análise , Espectrometria de Massas , Extratos Vegetais/química , Ácido Salicílico/análise
12.
J Food Sci ; 86(3): 740-748, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33580510

RESUMO

Kombucha is a black tea-based, non-alcoholic beverage fermented by yeast and bacteria are known for its refreshing scent and taste and presents biological characteristics, namely antioxidant, antimicrobial and anti-inflammatory activity. The present study compared traditional kombucha prepared with black tea and green tea to kombuchas produced with several alternative substrates, including white tea, chrysanthemum, honeysuckle, and mint infusions. Throughout the fermentation process, liquid and gas chromatography analyzed sugars, ethanol, organic acids, and volatile compounds. Sugar consumption was substrate-dependent, with mint kombucha having the highest amount of residual sugar and honeysuckle having the lowest. Forty-six volatile organic compounds were detected, including alcohols, esters, acids, aldehydes, ketones, and other compounds. Twenty-two compounds were produced during the fermentation and identified in all kombuchas; some of these compounds represented fruity and floral aromas. Another 24 compounds were substrate specific. Notably, the herb-based kombuchas (chrysanthemum, honeysuckle, and mint) contained several compounds absent in the tea-based kombuchas and are associated with minty, cooling, and refreshing aromas. Mint and green tea kombucha attained the highest and lowest overall sensorial acceptance ratings, respectively. This study demonstrated herbal substrates' suitability to prepare kombucha gastronomically with volatile compound and flavor profiles distinct from tea-based kombuchas. PRACTICAL APPLICATION: The kombucha beverage is a low-caloric functional drink that is increasingly popular around the world. While it is traditionally produced with black or green tea, this paper explores its production based on other herbal and floral infusions. The kombucha analogs presented in this paper can provide consumers with healthy alternatives for sugary soft drinks while also offering a broader range of flavors.


Assuntos
Chá de Kombucha/análise , Chás de Ervas/análise , Anti-Infecciosos/análise , Anti-Inflamatórios/análise , Antioxidantes/análise , Bebidas/análise , Camellia sinensis/química , Etanol/análise , Fermentação , Sensação , Açúcares/análise , Paladar , Chá/química , Compostos Orgânicos Voláteis/análise
13.
Molecules ; 26(3)2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33499197

RESUMO

Teas and various herbal teas are well-known beverages and are commonly consumed around the world. In this study, we focused on kuromoji tea. Kuromoji is a deciduous shrub of the Lauraceae family, and the plucked leaves and branches have been drunk as a tea in production areas for a long time. However, no studies have investigated the subjective and physiological effects of kuromoji tea. In this study, the effects of kuromoji tea were examined on the basis of the measurements of heart rate variability and cerebral blood flow, core body temperature and subjective assessments. Moreover, the results of this study showed that a pleasant subjective feeling could be obtained by sniffing the aroma of kuromoji teas, especially tea leaves. It was also found that the aroma of kuromoji teas has the potential to stimulate saliva secretion and increase subjective and physiological excitements in the oral cavity. 1,8-Cineole, linalool, terpinen-4-ol, carvone and geraniol were determined in both kuromoji leaves and branches. In this study, the beneficial effects of kuromoji teas when drunk conventionally were investigated.


Assuntos
Lindera/química , Odorantes/análise , Chás de Ervas/análise , Temperatura Corporal , Estudos Cross-Over , Cromatografia Gasosa-Espectrometria de Massas , Frequência Cardíaca , Humanos , Inalação , Japão , Masculino , Percepção Olfatória/fisiologia , Oxiemoglobinas/metabolismo , Microextração em Fase Sólida , Escala Visual Analógica , Adulto Jovem
14.
Food Chem ; 346: 128929, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33450644

RESUMO

Linden trees are a source of food products called lime flowers (Tiliae flos), traditionally used in the form of infusion for the treatment of feverish colds and coughs. Lime flowers should include flowers of Tilia cordata Mill, T.x europaea L., and T. platyphyllos Scop. or a mixture of these. The aim of current research was to establish a fast, sensitive HPTLC (high-performance thin-layer chromatography) method that would allow the differentiation of material obtained from five species of lime occurring in Europe. The fingerprints for distinguishing these species were established, as well as a key for identification based on a visual evaluation of chromatograms. The results obtained were also subjected to chemometric analyses. It was shown that each species contains characteristic compounds i.e. linarin that can be used for their identification. The method developed can, in theory, be introduced for the quality control or authentication of linden flowers on the European market.


Assuntos
Cromatografia em Camada Delgada , Flores/química , Análise de Alimentos/métodos , Chás de Ervas/análise , Tilia/química , Controle de Qualidade
15.
Clin Toxicol (Phila) ; 59(8): 746-755, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33435745

RESUMO

OBJECTIVE: To assess characteristics of exposures to contaminated poppy and identify trends in exposure and poppy-related deaths. METHODS: Cross-sectional analysis of adverse events associated with exposure to poppy products (primarily poppy seeds) from the American Association of Poison Control Centers' National Poison Data System (NPDS), 2000-2018, supplemented with analysis of overdoses and deaths related to poppy from the U.S. Food and Drug Administration (FDA) Center for Food Safety and Applied Nutrition's Adverse Event Reporting System (CAERS) (2004-2018), and the FDA Adverse Event Reporting System (FAERS) (1968-2018). RESULTS: There were 591 NPDS exposure cases involving poppy between 2000 and 2018 including 392 in persons aged 13+. Rates of intentional exposures in NPDS increased among the age 13+ group over the study period. Most intentional exposures occurred in males in their teens and twenties. NPDS included 18 overdoses and three deaths likely attributable to poppy, most involving poppy seed tea. CAERS and FAERS included five additional deaths likely attributable to opioids in poppy. CONCLUSIONS: Including previously reported cases, there are now at least 19 U.S. deaths associated with poppy seeds in the literature. We recommend that practitioners working in opioid treatment and recovery be alert to use of poppy to treat pain and symptoms of withdrawal.


Assuntos
Papaver , Chás de Ervas/envenenamento , Adolescente , Adulto , Criança , Exposição Dietética/estatística & dados numéricos , Overdose de Drogas/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Centros de Controle de Intoxicações/estatística & dados numéricos , Sementes , Estados Unidos/epidemiologia , United States Food and Drug Administration/estatística & dados numéricos , Adulto Jovem
16.
Food Chem ; 342: 128175, 2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33097332

RESUMO

Although aged fragrance is the most outstanding quality characteristic of dark tea, its formation still is not much clear. Thus, the volatiles of Qingzhuan tea (QZT) during the whole post-fermentation process were investigated at an industrial scale. The results showed that most of volatiles increased during pile-fermentation of QZT and weakened during aging storage, but some new volatiles were produced through aging storage. Hexanal, (E)-2-hexenal, (E)-2-decenal, 2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde, heptanal, (E)-2-octenal, (R)-5,6,7,7a-tetrahydro-4,4,7a-trimethyl-2(4H)-benzofuranone, ionone, 2-heptanone, 3-ethyl-4-methyl-1H-pyrrole-2,5-dione, (R,S)-5-ethyl-6-methyl-3-hepten-2-one, cis-5-ethenyltetrahydro-5-trimethyl-2-furanmethanol, and linalool generated by pile-fermentation should be the basic volatiles of aged fragrance in QZT, and 4-(2,4,4-trimethyl-cyclohexa-1,5-dienyl)-but-3-en-2-one, 6-methyl-5-heptene-2-one, safranal, guaiene, trans-2-(2-propynyloxy)-cyclohexanol, nonanal, and 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butanone formed during aging storage should be the transformed volatiles of aged fragrance in QZT, which together constitute the characteristic components of aged fragrance. Notably, 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butanone, 6-methyl-5-heptene-2-one, and safranal were selected as the key volatiles of QZT. These results contribute to understand better the formation of agedfragrance in dark tea.


Assuntos
Chás de Ervas/análise , Compostos Orgânicos Voláteis/análise , Análise por Conglomerados , Análise Discriminante , Fermentação , Armazenamento de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Análise dos Mínimos Quadrados , Microextração em Fase Sólida , Fatores de Tempo , Compostos Orgânicos Voláteis/isolamento & purificação
17.
Strahlenther Onkol ; 197(1): 63-73, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32970162

RESUMO

PURPOSE: To determine the effect of Cystus® tea (Naturprodukte Dr. Pandalis GmbH & Co. KG) as mouthwash compared to sage tea on oral mucositis in patients undergoing radio(chemo)therapy for head and neck cancer. METHODS: In this randomized, prospective phase III study, 60 head and neck cancer patients with primary or postoperative radio(chemo)therapy were included between 04/2012 and 06/2014. They received either sage or Cystus® tea for daily mouthwash under therapy. Mucositis was scored twice a week following the Radiation Therapy Oncology Group and the European Organization for Research and Treatment Cancer (RTOG/EORTC) scoring system. Dental parameters were also recorded. Statistical evaluation of the primary endpoint was performed using t­test and log rank test. RESULTS: Data from 57 patients could be evaluated. Patient characteristics showed no significant difference between the two groups (n = 27 sage; n = 30 Cystus®). A total of 55 patients received the prescribed dose (60-66 Gy postoperative; 70-76.8 Gy primary). Mucositis grade 3 was observed in 23 patients (n = 11 sage; n = 12 Cystus®) and occurred between day 16 and 50 after start of therapy. There was no significant difference between the two groups in latency (p = 0.75) and frequency (p = 0.85) of the occurrence of mucositis grade 3. The self-assessment of the oral mucosa and the tolerability of the tea also showed no significant differences. Occurrence of dental pathologies appeared to increase over time after radiotherapy. CONCLUSION: Cystus® and sage tea have a similar effect on the occurrence of radiation-induced mucositis regarding latency and incidence. Cystus® tea mouthwash solution is tolerated well and can be applied in addition to intensive oral care and hygiene along with the application of fluorides.


Assuntos
Cistaceae/química , Neoplasias de Cabeça e Pescoço/radioterapia , Antissépticos Bucais/uso terapêutico , Fitoterapia , Polifenóis/uso terapêutico , Lesões por Radiação/prevenção & controle , Estomatite/prevenção & controle , Chás de Ervas , Idoso , Idoso de 80 Anos ou mais , Quimiorradioterapia/efeitos adversos , Índice CPO , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Estudos Prospectivos , Lesões por Radiação/tratamento farmacológico , Lesões por Radiação/etiologia , Índice de Gravidade de Doença , Estomatite/tratamento farmacológico , Estomatite/etiologia , Fatores de Tempo
18.
Biol Trace Elem Res ; 199(2): 769-778, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32361885

RESUMO

The feasibility of using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) to overcome spectral overlaps in the determination of non-metallic elements was investigated. The contents of Si, P, S, Cl, Br, and I in herbal tea were determined by using ICP-MS/MS after microwave digestion. In the MS/MS mode, O2 and H2 were consecutively used as reaction gases. Low background equivalent concentration (BEC) and limit of detection (LOD) of analytes were obtained when using O2 mass shift, H2 mass shift, and H2 on-mass methods. The LODs for Si, P, S, Cl, Br, and I were 0.41, 0.048, 0.34, 0.76, 0.055, and 0.007 µg L-1, respectively. Standard reference materials NIST SRM 1515 (apple leaves) and NIST SRM 1547 (peach leaves) were used to evaluate the accuracy of the analytical method. The developed method was used to analyze 20 herbal teas. The ranges of values for Si, P, S, Cl, Br, and I in herbal tea were 236-4100, 1830-4360, 1290-3850, 335-4620, 0.86-8.21, and 0.091-0.65 µg g-1, respectively. The results showed that several non-metallic elements essential for the human body might be obtained by drinking herbal tea.


Assuntos
Espectrometria de Massas em Tandem , Chás de Ervas , Humanos , Limite de Detecção , Micro-Ondas , Análise Espectral
19.
J Insect Physiol ; 128: 104177, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33279470

RESUMO

Animal-pollinated plants face a common problem, how their defensive anti-herbivore compounds may impair or alter pollinator behavior. Evolution has tailored multiple solutions, which largely involve pollinator tolerance or manipulation, to the benefit of the plant, not the removal of these compounds from pollen or nectar. The tea plant, Camilla sinensis, is famous for the caffeine and tea polyphenols (TP) that it produces in its leaves. However, these compounds are also found in its nectar, which honey bees readily collect. We examined the effects of these compounds on bee foraging choices, learning, memory, and olfactory sensitivity. Foragers preferred a sucrose feeder with 100 µg or 10 µg TP/ml over a control feeder. Caffeine, but not TP, weakly increased honey bee learning. Both caffeine and TP significantly increased memory retention, even when tested 7 d after the last learning trial. In addition, TP generally elevated EAG responsiveness to alarm pheromone odors. These results demonstrate that other secondary plant compounds, not only caffeine, can attract pollinators and influence their learning and memory.


Assuntos
Abelhas/fisiologia , Polifenóis/farmacologia , Olfato , Animais , Comportamento Alimentar/efeitos dos fármacos , Memória/efeitos dos fármacos , Odorantes , Extratos Vegetais/farmacologia , Néctar de Plantas/farmacologia , Olfato/efeitos dos fármacos , Olfato/fisiologia , Chás de Ervas
20.
Food Chem ; 344: 128738, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33280962

RESUMO

This study aimed to compare the effect of fermentation and drying on the organoleptic characteristic, total phenolic content, antioxidant and anti-inflammatory activities of Peperomia pellucida (L.) Kunth tea with commercial Camellia sinensis tea. The phenolic content, antioxidant and anti-inflammatory activities in P. pellucida were significantly (p < 0.05) lower than C. sinensis, irrespective of the fermentation and drying methods. Although fermentation decreased the total phenolics, flavonoids and antioxidant activity in both P. pellucida and C. sinensis teas, the anti-inflammatory potential of P. pellucida was significantly (p < 0.05) improved. Principle component analysis revealed that fermentation and drying methods contributed to respective 42.3% and 27.2% of activity variation in P. pellucida. The browning index was positively correlated with fermentation index (r = 0.670, p < 0.05) of leaves samples. Overall, unfermented and fermented P. pellucida leaves were best dried with microwaving and freeze drying, respectively for optimal antioxidant and anti-inflammatory activities with favorable consumer's acceptance.


Assuntos
Anti-Inflamatórios/análise , Antioxidantes/análise , Dessecação/métodos , Fermentação , Peperomia/química , Chás de Ervas/análise , Flavonoides/análise , Fenóis/análise , Folhas de Planta/química
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