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1.
Methods Mol Biol ; 2578: 237-247, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36152292

RESUMO

Phage display is a molecular biology cloning technique that allows the expression of genes of interest along with the phage surface protein. The technique described for the following method used a genomic library for the expression of peptides composed of 12 amino acids, with the objective of selecting peptides which presented specific affinity to the molecules of interest. As a target, purified extracellular vesicles from cell cultures of cells 5637 and RT4 were chosen, which in turn have enormous application and can help to understand the functioning of bladder cancer, allowing the development of new vaccines, drugs, therapies, and diagnoses.


Assuntos
Bacteriófagos , Vesículas Extracelulares , Vacinas , Aminoácidos/metabolismo , Bacteriófagos/genética , Bacteriófagos/metabolismo , Vesículas Extracelulares/genética , Proteínas de Membrana/metabolismo , Biblioteca de Peptídeos , Peptídeos/química , Tecnologia , Vacinas/metabolismo
2.
Methods Mol Biol ; 2577: 103-122, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36173569

RESUMO

The Spliced TetO REpeAt, MS2 repeat, and INtein sandwiched reporter Gene tag (STREAMING-tag) system enables imaging of nuclear localization as well as the transcription activity of a specific endogenous gene at sub-100-nm resolution in living cells. The use of this system combined with imaging of epigenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between Nanog gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.


Assuntos
Fatores de Transcrição , Transcrição Genética , Animais , Camundongos , Células-Tronco Embrionárias Murinas , Proteínas Nucleares , Tecnologia , Fatores de Transcrição/genética
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121779, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36041262

RESUMO

Online monitoring of antibiotics in the environment attracts more and more attention. Surface-enhanced Raman scattering (SERS) is a promising technique for the detection of trace amounts of antibiotics in the environment, which is fast, non-invasive and sensitive. To investigate the enrichment of trace amounts of antibiotics in water, polyethylene microplastics (PE MPs) were prepared as sorbents to simply concentrate enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan in water, followed by the SERS measurement of antibiotics extract washed from MPs on an AgNPs@Si SERS substrate. Limit of detection of Rhodamine 6G is 2.1 × 10-12 M achieved from the AgNPs@Si SERS, indicating a high enhancement. The detection results show that SERS peaks of the antibiotics could be observed from the spectra of the extracts eluted from MPs, indicating MPs could adsorb and desorb antibiotics from water. Besides, for enrofloxacin and triclosan, the intensity of SERS measured from the MPs extracts are higher than that of directly from the spiked water, demonstrating the proposed method could lower the detectable concentration of hydrophobic antibiotics in water. Moreover, the proposed MPs sorbents combined with SERS method was applied to detect the antibiotics in real river water, with minimal detection of 10-10 M, 10-8 M, and 10-8 M achieved for enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan, respectively. The proposed method provides a promising simple, rapid and low reagent consuming means for monitoring antibiotics in water.


Assuntos
Antibacterianos , Triclosan , Antibacterianos/análise , Ciprofloxacina , Enrofloxacina , Microplásticos , Plásticos , Polietilenos , Prata/química , Análise Espectral Raman/métodos , Tecnologia , Água
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121785, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36058172

RESUMO

Eating repeatedly used hotpot oil will cause serious harm to human health. In order to realize rapid non-destructive testing of hotpot oil quality, a modeling experiment method of fluorescence hyperspectral technology combined with machine learning algorithm was proposed. Five preprocessing algorithms were used to preprocess the original spectral data, which realized data denoising and reduces the influence of baseline drift and tilt. The feature bands extracted from the spectral data showed that the best feature bands for the two-classification model and the six-classification model were concentrated between 469 and 962 nm and 534-809 nm, respectively. Using the PCA algorithm to visualize the spectral data, the results showed the distribution of the six types of samples intuitively, and indicated that the data could be classified. Based on the modeling analysis of the feature bands, the results showed that the best two-classification models and the best six-classification models were MF-RF-RF and MF-XGBoost-LGB models, respectively, and the classification accuracy reached 100 %. Compared with the traditional model, the error was greatly reduced, and the calculation time was also saved. This study confirmed that fluorescence hyperspectral technology combined with machine learning algorithm could effectively realize the detection of reused hotpot oil.


Assuntos
Algoritmos , Máquina de Vetores de Suporte , Fluorescência , Humanos , Aprendizado de Máquina , Tecnologia
5.
Food Chem ; 398: 133849, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35985240

RESUMO

A promising drying technology, namely combined freeze-drying and far-infrared drying (FD-FIRD) was employed to dehydrate seed-used pumpkin (SUP) and its effects on physical characteristics (color, microstructure, texture, shrinkage), bioactive components (phenolics and carotenoids) of SUP were evaluated. The low-field nuclear magnetic resonance (LF-NMR) was used to analyze moisture state and distribution during FD process, which is beneficial for choosing appropriate moisture transfer point. Results showed that most free water (87.29 %) was removed when drying time reached 25 h, thus retaining original tissue structure of sample with almost no deformation during later FIRD process. Therefore, FD for 25 h followed by FIRD for 2 h (FD25-FIRD2) sample exhibited similar appearance with FD sample. The free phenolics content of FD-FIRD samples was 14.97 %-26.60 % higher than that of FD depending on FIRD duration. Especially, p-coumaric acid content of FD25-FIRD2 sample increased of 32.23 %. Besides, FD-FIRD performed higher retention for carotenoids showing 3.00-3.39 folds than FIRD.


Assuntos
Cucurbita , Antioxidantes/química , Carotenoides/análise , Fenóis/análise , Sementes/química , Tecnologia
6.
Food Chem ; 398: 133853, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35988412

RESUMO

Plant-based phenolic extracts have gained significant attention in the food industry due to their antimicrobial and health-promoting effects. However, their usage is limited because of poor water solubility and instability during processing. Therefore, encapsulation of phenolics with a suitable carrier system is essential for overcoming these problems and increasing their application in food products. In this study, encapsulated phenolic extracts were used for the first time in vacuum impregnation (VI). For this purpose, different phenolic extracts (cinnamon, turmeric, pomegranate peel) were obtained from the plant source. PPE was selected because it has the highest total phenolic content, antioxidant capacity, and antimicrobial activity against Botrytis cinerea. Then, PPE was encapsulated with different emulsifiers (T80, GMO, IN, WPI, and LEC). After the characterization and stability studies were performed, PPE encapsulated with T80 was used to produce a functional strawberry snack by VI technology. The results showed that the diffusion rate of EPPE was significantly increased compared to the control and PPE-VI group. EPPE-enriched strawberries were the preferred snack with high-quality characteristics.


Assuntos
Fragaria , Antioxidantes , Fenóis/análise , Extratos Vegetais , Tecnologia , Vácuo
7.
Food Chem ; 399: 133887, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36001929

RESUMO

Although the demand for meat analogues is increasing, aspects of the analogues' textural characteristics continue to be problematic. To solve these problems, a new technique for applying vacuum packaging and pressurized heat (i.e., vacuum-autoclaving) to low-moisture TSP has been proposed. An analysis of the morphological characteristics of the analogues showed that the vacuum-autoclaving treatment affected the packing structure of the materials. The density was increased by about 0.25 g/mL by vacuum-autoclaving, and the movement of water was restricted by the formation of a packed structure. The disulfide bonds increased by 3 µM/g or more and the ß-sheets and α-helixes increased by 7 % or more; this contributed to structural changes due to protein aggregation. The texturization index and hardness tended to decrease by more than 30 %, indicating a decrease in overall structural strength. The newly proposed technology has potential for use in the commercialization of meat analogues.


Assuntos
Embalagem de Alimentos , Proteínas de Soja , Embalagem de Alimentos/métodos , Temperatura Alta , Carne/análise , Tecnologia , Vácuo
8.
Chemosphere ; 310: 136729, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36252900

RESUMO

Vacuum drying is an effective approach for sludge treatment and valorization. However, the vacuum drying of sludge has not been industrialized at present. The objective of this study was to elucidate the vacuum drying characteristics of static sludge and crack initiation mechanism. Our results indicate that crusting on the sludge surface under a high vacuum inhibited drying by reducing major cracks at sludge thicknesses of 13.6 and 10.2 mm. The inhibition effect weakened with decreasing sludge thickness. At 6.8 mm, the mean drying rate (VM) was the lowest at 0.08 MPa, while VM decreased with increasing vacuum degree at thicknesses of 13.6 and 10.2 mm. The decrease in drying rate could be attributed to rapid evaporation on the sludge surface under a high vacuum, leading to crusting, which inhibited crack initiation. VM was raised by 67.9-162.2% from 10.2 to 6.8 mm because the suction force of vacuum on water was much higher than the resistance to water diffusion of small isolation piles at 6.8 mm. Additionally, this study provided essential information to improve existing sludge treatment methods.


Assuntos
Dessecação , Esgotos , Vácuo , Dessecação/métodos , Água , Tecnologia
9.
Methods Mol Biol ; 2575: 181-193, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36301476

RESUMO

Currently, the only feasible option for patients with progressive and/or end-stage organ degeneration is to undergo transplantation. Due to the growing unmatched demand of available organ donors and, as a consequence, the continuous growth of patients' waiting lists, the development of new tissue engineering technologies is a relevant need. In this chapter, we will focus on the liver as a model organ to discuss contemporary tissue engineering strategies. Induced pluripotent cells are an attractive alternative to serve as a cell source for tissue engineering applications due to their pluripotency, the potentiality to generate autologous transplantation, and for their high proliferation rate. Among the main liver tissue engineering technologies, 3D bioprinting, hepatic organoids, and decellularization/recellularization of biological matrixes have grown much attention as alternatives to derive functional liver grafts. Thus, this chapter will discuss how recent publications have demonstrated the use of induced pluripotent cells in the development of the aforementioned technologies. Bioprinting is an additive manufacturing biofabrication process where cells are dispersed within a matrix formulation (i.e., bioink) and extruded in a modified 3D-printer. Polymers within bioink can be cross-linked to increase stiffness. Hepatic spheroids showed greater viability and liver function, due to preserved epithelial phenotype over time. Organoid is multi-lineage tissue constructs derived from a stem cell that recapitulates the early stages of organogenesis. The influence of cellular composition of non-parenchymal cells using induced pluripotent-derived cells or primary adult cells for hepatic organoid formation was recently tested. Decellularization is a process where harvested tissues or organs are washed with a detergent-based solution, to lyse and remove all cellular components. The final product is an extracellular scaffold with preserved tissue vasculature and ultra-structure, which can be used for subsequent recellularization with recipient cells. This chapter sheds light on recent works on the use of induced pluripotent-derived cells for liver tissue engineering approaches and on how such technologies could potentially generate therapeutic alternatives for patients on waiting lists for liver transplantation.


Assuntos
Proliferação de Células , Hepatócitos , Células-Tronco , Engenharia Tecidual , Bioimpressão , Proliferação de Células/fisiologia , Hepatócitos/fisiologia , Transplante de Órgãos , Impressão Tridimensional , Células-Tronco/fisiologia , Tecnologia , Engenharia Tecidual/métodos
10.
Methods Mol Biol ; 2585: 105-117, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36331769

RESUMO

The mosquito-borne West Nile virus (WNV) poses a great threat to public health as no vaccine or specific antiviral treatment is available. Exploring virus-host interactions, specifically host factors that are required for virus infection, is important for better understanding the biology, pathogenesis, and transmission of WNV. Such essential host factors may also represent antiviral targets. The development of CRISPR technology has provided a powerful and convenient tool to perturbate host gene expression, allowing for unbiased, genome-wide screens of host factors for virus infection. Here we describe the necessary steps for performing a CRISPR knockout screen, which can also be applied to other viruses, to identify host factors critical for WNV infection.


Assuntos
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Vírus do Nilo Ocidental , Animais , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética , Interações entre Hospedeiro e Microrganismos , Vírus do Nilo Ocidental/genética , Antivirais , Tecnologia
11.
Methods Mol Biol ; 2562: 461-469, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36272094

RESUMO

The dramatic increase in accessibility to sequencing technologies has opened new avenues into studying different processes, cells, and animal models. In the amphibian models used for regeneration research, these new datasets have uncovered a variety of information about what genes define the regenerating limb as well as how genes and cells change over the course of regeneration. The accumulation of data from these studies undoubtedly increases our understanding of regeneration. Throughout these studies, it is important to consider how data can be made most useful not only for the primary study but also for reuse within the scientific community. This chapter will focus on best practices for data collection and handling as well as principles to promote access and reuse of big datasets. However, the deposition and thorough description of data of all sizes generated for a publication (e.g., images, fcs files, etc.) can also be done following this generic workflow. The aim is to lower hurdles for reuse, access, and re-evaluation of data which will in turn increase the utility of these datasets and accelerate scientific progress.


Assuntos
Tecnologia , Fluxo de Trabalho , Análise de Sequência , Coleta de Dados
12.
J Environ Manage ; 325(Pt A): 116471, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36244290

RESUMO

Identifying key drivers of manufacturing CO2 emissions is critical to carbon reduction practices. For manufacturing, CO2 emissions are mainly determined by production capacity and production scale. However, traditional production-theoretical decomposition analysis (PDA) fails to consider production-scale-related drivers. To better support policy development and implementation, this paper improves PDA based on industrial linkage theory. The improved model can identify seven production-capacity-related drivers and five production-scale-related drivers, allowing a comprehensive understanding of CO2 emission drivers. Then this model is implemented to investigate CO2 emission changes in 18 manufacturing sectors in Hubei Province, China, from 2012 to 2017. Results show that manufacturing CO2 reduction efforts in Hubei Province have yielded some achievements, with reduced potential energy intensity and improved CO2 emission technical efficiency in most sectors. Changes in external market demand and final demand structure have contributed to CO2 reduction in most sectors. Results also reveal some problems in manufacturing in Hubei Province, such as the inability to improve CO2 emission technical efficiency and CO2 emission technology strength, the slow improvement of energy utilization technical efficiency and energy utilization technology strength, and the reduction of value-added rate.


Assuntos
Dióxido de Carbono , Indústrias , Dióxido de Carbono/análise , Comércio , Carbono/análise , Tecnologia , China , Desenvolvimento Econômico
13.
J Environ Manage ; 325(Pt A): 116502, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36274310

RESUMO

Although the past twenty years have witnessed China's remarkable economic development, the cost in terms of greenhouse gas emissions and a deteriorating environment has been enormous. Numerous studies have revealed the influence of household factors on household carbon dioxide emissions (HCEs) and called for a reduction of HCEs to mitigate climate change, but few have focused on assessing the most significant household driving factors of HCEs. Using statistical data between 2005 and 2019 in Jiangsu, China, this study developed an extended stochastic impact by regression on population, affluence, and technology (STIRPAT) model to assess the most significant driving factors of HCEs. The results show that the most significant driving factors are household size, total population, unemployment, and urbanisation rate. The study found that HCEs are positively impacted by household size while negatively impacted by the unemployment rate. Based on the study's findings, the following suggestions are proposed to lower HCEs: (i) establish an optimal consumption concept to guide residents towards consuming reasonably; (ii) cultivate a low-carbon concept among residents and promote low-carbon emissions living; and (iii) pay close attention to population structure factors and formulate effective measures accordingly. The study provides insightful information on the key driving factors of HCEs, which can facilitate achieving carbon emissions neutrality.


Assuntos
Dióxido de Carbono , Gases de Efeito Estufa , Dióxido de Carbono/análise , Desenvolvimento Econômico , China , Gases de Efeito Estufa/análise , Tecnologia
14.
Curr Probl Cardiol ; 48(1): 101415, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36155199

RESUMO

Coronary artery disease (CAD) is a cardiovascular disease of the blood vessels that makes vessels, narrow and hardened and difficult to supply blood to the heart. The epidemiology of CAD disease is a common clinical syndrome of a global health priority and the burden is increasing at an alarming rate worldwide. The prevalence of CAD not only increases mortality, morbidity and worsens the patient quality of life but also puts a huge burden on the overall healthcare system. The novel risk factors include: cholesterol level, cigarette smoking, diabetics, obesity, and hypertension, respectively are the causative agents of CAD. Furthermore, the etiology of CAD is also a very complex process and several interrelated etiological factors are involved in the pathogenesis of CAD. The signs and symptoms of CAD appear like angina, heart failure, and dyspnea, myocardial infarction, and arrhythmia, respectively. The management and diagnosis of CAD include different types of medications that are used nowadays for the treatment of this disease. The highlights of the present review focused on stent technology and its useful applications. Finally, we also addressed the benefits of the stent, and its potential complications, effectiveness, indication, and contraindication that play a significant role in the recovery of CAD disease.


Assuntos
Doença da Artéria Coronariana , Infarto do Miocárdio , Humanos , Doença da Artéria Coronariana/diagnóstico , Doença da Artéria Coronariana/epidemiologia , Doença da Artéria Coronariana/etiologia , Qualidade de Vida , Stents , Fatores de Risco , Tecnologia
15.
Sci Total Environ ; 856(Pt 2): 159122, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36183772

RESUMO

Heterogeneous photocatalysis using titanium dioxide-based materials is considered a promising and innovative solution to the water pollution problem. However, due to the limitations concerning the use of the developed materials and the applied photodegradation conditions, the research on photoremediation using TiO2 often stays behind the lab door. The challenge is to convert the basic research into a successful innovation, leading to the implementation of this process into wastewater treatment. For this purpose, the most active materials and optimal photodegradation conditions must be chosen. This article collects and compares the studies on photocatalytic degradation of an emerging pollutant - sulfamethoxazole, an antibacterial drug - and attempts to find the best approaches to be successfully applied on an industrial scale. Various types of TiO2-based photocatalysts are compared, including different nanoforms, doped or polymer-based composites, composites with graphene, activated carbon, dyes or natural compounds, as well as possible supporting materials for TiO2. The paper covers the impact of the irradiation source (natural sunlight, LED, mercury or xenon lamps) and water matrix on the photodegradation process, considering the ecological and economic sustainability of the process. Emphasis is put on the stability, ease of separation and reuse of the photocatalyst, power and safety of the irradiation source, identification of photodegradation intermediates and toxicity assays. The main approaches are critically discussed, main challenges and perspectives for an effective photocatalytic water treatment technology are pointed out.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Sulfametoxazol , Catálise , Titânio , Tecnologia
16.
Sci Total Environ ; 857(Pt 2): 159494, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36257411

RESUMO

Anaerobic digestion has been employed as a technology capable of adding value to waste coupled with environmental impact mitigation. However, many issues need to be elucidated to ensure the systems viability based on this technology. In this sense, the present study evaluated technically, environmentally, and economically, four configurations of swine waste treatment systems focused on the promotion of decarbonization and circularity of the swine chain. For this, a reference plant, based on a compact treatment process named SISTRATES® (Portuguese acronym for swine effluent treatment system) was adopted to serve as a model for comparison and validation. The results showed the importance of prioritization of the energy recuperation routes through anaerobic digestion, providing increased economic benefits and minimizing environmental damage. Thus, the SISTRATES® configuration was the one that presented the best designs in a circular context, maximizing the recovery of energy and nutrients, along with the reduction of greenhouse gas emissions, ensuring the sustainability of the pig production chain.


Assuntos
Gases de Efeito Estufa , Esterco , Suínos , Animais , Esterco/análise , Conservação dos Recursos Naturais , Tecnologia , Estágios do Ciclo de Vida , Anaerobiose
17.
J Exp Child Psychol ; 226: 105573, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36332435

RESUMO

Adolescents use social identities and reasoning to make peer inclusion and attribution decisions. School climate plays a role in these decisions. Thus, this study analyzed how school racial climate and STEM (science, technology, engineering, and math) classroom climate were associated with the choices of adolescents (N = 294; Mage = 15.72 years; 52.3 % female; 36.7 % White/European American, 32.9 % Black/African American, 11.2 % Latino/Hispanic [the most common racial/ethnic groups in the schools where data collection took place]) in two tasks: peer inclusion and attribution of ability. On the peer inclusion task, participants were more likely to choose a non-White peer for a STEM activity if they had lower perceptions of stereotyping at school, and they were more likely to choose a female peer if they were female. Participants were more likely to use reasoning based on personal characteristics when choosing a peer, but female participants who chose a female peer were more likely to use reasoning based on gender. On the attribution task, participants were more likely to choose a non-White peer if they perceived greater STEM connectedness, and they were more likely to choose a White or male peer if they had more positive relationships with their STEM teachers. Therefore, students' perceptions of school racial climate relate to adolescents' peer inclusion decisions, and their perceptions of STEM classroom climate relate to adolescents' ability attributions. Schools may need to focus on creating welcoming school and classroom environments as a way to promote equity in STEM.


Assuntos
Engenharia , Tecnologia , Feminino , Masculino , Adolescente , Humanos , Grupos Raciais , Instituições Acadêmicas , Matemática
18.
Pers Soc Psychol Bull ; 49(1): 138-151, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34906005

RESUMO

In the era of technology, smartphone use occupies an important position in our lives. The present research focused on the psychological consequence of frequent smartphone use and possible way to remedy it. We proposed that frequent smartphone use could damage people's sense of control and in turn trigger nostalgia. Moreover, nostalgia could directly compensate for the low sense of control induced by frequent smartphone use. Five studies (N = 918) were conducted. Study 1 found through a field study that frequent smartphone use increases nostalgia. Studies 2 and 3 found through 14-day tracking and a laboratory experiment that frequent smartphone use decreased people's sense of control and then triggered nostalgia. Furthermore, nostalgia could enhance the low sense of control, and it worked by increasing self-esteem (Studies 4 and 5). The findings show the negative impact of frequent smartphone use, and nostalgia is an effective way to remedy it without preventing people from using smartphones.


Assuntos
Emoções , Smartphone , Humanos , Autoimagem , Tecnologia
19.
J Affect Disord ; 320: 1-6, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36162664

RESUMO

BACKGROUND: Measurement-based care (MBC) is an evidence-based approach that can improve management of depression, but its use in clinical settings remains low. The use of digital technology, or technology-enhanced MBC (eMBC), may address some of the barriers to implementation of MBC by enabling patients to easily complete outcome assessments. This study aims to identify the perceived facilitators and barriers to MBC and eMBC from both patient and provider perspectives in Canada. METHODS: The study recruited 108 clinicians and 131 patients with a self-reported diagnosis of depression to participate in an online survey about the acceptability of both MBC and eMBC. RESULTS: Most clinicians (90/108, 83.3 %) agreed that MBC is important for clinical judgement, and felt trained to interpret their scores; however, only 43/108 (39.8 %) use MBC routinely. Clinicians (95/108, 88.0 %) felt they would be more likely to use MBC if it was automated and available to use electronically. Most patients (117/131, 89.3 %) felt MBC would be helpful for their treatment and were willing to use a mobile app to track their symptoms. LIMITATIONS: Recruitment was done online and, hence, this study may not capture the perspective of patients who are not already familiar with using online programs to complete questionnaires. CONCLUSIONS: Clinicians and patients in Canada would be willing to utilize MBC and eMBC in their psychiatric care, but barriers to implementation include perceptions about efficiency, ease of use, and accessibility. These results highlight the importance of engaging both clinicians and patients to effectively implement MBC and eMBC for depression.


Assuntos
Depressão , Aplicativos Móveis , Humanos , Depressão/diagnóstico , Depressão/terapia , Depressão/psicologia , Canadá , Inquéritos e Questionários , Tecnologia
20.
Food Chem ; 403: 134367, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36358067

RESUMO

A pulsed electric field (PEF) induces cell electroporation for solid foods, thereby allowing PEF to be used as a pretreatment method for extraction, drying, peeling, freeze-thawing, and cooking by increasing mass transfer. Currently, popular research mainly focuses on the process and results of the application of PEF to solid food processing. Therefore, this review summarizes the impact of PEF on the quality of solid food, the evaluation techniques of PEF-treated tissues and the characteristics of PEF treatment chambers. Furthermore, other pretreatments, including freezing and peeling, typically used in the meat and vegetable sectors, are also discussed alongside PEF to evaluate the impact on its effects. Finally, this article examined the main obstacles and prospects of PEF in solid food processing. This evaluation is anticipated to expand future PEF research paths in the solid food industry.


Assuntos
Eletricidade , Manipulação de Alimentos , Manipulação de Alimentos/métodos , Indústria de Processamento de Alimentos , Carne/análise , Tecnologia
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