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1.
Microb Drug Resist ; 28(9): 921-934, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36067095

RESUMO

This study aimed to determine the prevalence and risk factors associated with intestinal carriage of extended-spectrum ß-lactamases producing Enterobacterales (ESBL-PE) and plasmid-mediated AmpC ß-lactamase producing Enterobacterales (AmpC-PE) in healthy children in Ardabil, Iran. A total of 305 fecal samples were collected. Isolates underwent antimicrobial susceptibility testing, phenotypic and genotypic identification of ß-lactamase production, and epidemiologic molecular typing. In total, 21.5%, 1.5%, and 1.2% of volunteers were extended-spectrum ß-lactamase (ESBL)-, AmpC-, and simultaneous ESBL/AmpC-PE carriers, respectively. Escherichia coli was the predominant ESBL producing bacterium (70.2%) found in ESBL-PE colonized subjects. Beyond ESBL positive isolates, bla CTX-M group genes were the most common type (75.6%) and bla TEM (non-bla TEM-1 and non- bla TEM-2) were in the second place (25.6%). Among bla CTX-M genes, bla CTX-M-1 (55.3%) and bla CTX-M-15 (55.3%) were the most predominant types with equal prevalence. Some isolates were multi-enzyme producers. bla CIT and bla DHA genes were common AmpC type enzyme encoding genes found in AmpC-PE isolates. Most isolates produced both enzymes at the same time. The number of students in the classes was statistically associated with ESBL-PE intestinal carriage (p < 0.05). Moreover, 46 (65.7%), 3 (60%), 4 (100%), and 98 (39.8%) ESBL-, AmpC-, ESBL/AmpC, and non-ESBL/AmpC-PE isolates were multidrug-resistant, respectively. Overall, regardless of ß-lactam antibiotics, 62% and 59.5% of isolates were resistant to co-trimoxazole and tetracycline, respectively. The majority of ESBL producing E. coli isolates (69.2%) belonged to phylogroup A. According to Enterobacterial repetitive intergenic consensus polymerase chain reaction, there was no clonal relatedness between isolates. This study showed a high rate of multi-resistant ESBL-PE intestinal carriage among healthy individuals in Iran.


Assuntos
Infecções por Escherichia coli , Escherichia coli , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Criança , Resistência Microbiana a Medicamentos , Infecções por Escherichia coli/microbiologia , Humanos , Irã (Geográfico)/epidemiologia , Testes de Sensibilidade Microbiana , Epidemiologia Molecular , Prevalência , Fatores de Risco , Tetraciclinas , Combinação Trimetoprima e Sulfametoxazol , beta-Lactamases/genética , beta-Lactamas
2.
J Wound Care ; 31(Sup9): S45-S49, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36113855

RESUMO

OBJECTIVE: Diabetic foot ulcers (DFUs) are characterised by the presence of many microbes, some of which may not be identified by traditional culture techniques. Total contact casting (TCC) remains the gold-standard for offloading, yet little is known about the microbiome of wounds that progress from hard-to-heal to closed within a TCC. METHOD: A patient with a DFU underwent weekly treatment with TCC to closure. Samples for next-generation sequencing (NGS) and bioinformatics analysis of tissue samples were collected during each visit. Detection, identification, characterisation of the microbial community and abundance of microbes in each sample were compared. RESULTS: Abundance of microbes, identified by species and strain, changed with each treatment visit. By the final week of treatment, species diversity of the wound microbiome had decreased significantly, highlighted by an observed decrease in the number of total microorganisms present. Resistance genes for tetracyclines were detected in the first sample, but not in subsequent samples. CONCLUSION: The results of this study suggest dynamic microbiological changes associated with DFUs as they progress to healing within a TCC. As NGS becomes more readily available, further studies will be helpful to gain an improved understanding of the significance of the wound microbiome in patients with DFUs.


Assuntos
Diabetes Mellitus , Pé Diabético , Microbiota , Antibacterianos/uso terapêutico , Diabetes Mellitus/tratamento farmacológico , Pé Diabético/microbiologia , Humanos , Microbiota/genética , Tetraciclinas , Cicatrização
3.
Infect Dis Poverty ; 11(1): 98, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36114584

RESUMO

BACKGROUND: Antimicrobial resistance (AMR) is one of the greatest threats to animal and public health. Here, we conducted a dynamic surveillance of Escherichia coli on Chongming Island in Shanghai during 2009-2021 to identify the characteristics and trends of Chongming's AMR pandemic. METHODS: Rectal (cloaca) swabs from four poultry and nine swine farms (Chongming Island, 2009-2021) were collected for E. coli strains acquisition. The micro-broth dilution method was used to test antimicrobial susceptibility of E. coli isolates against 10 antimicrobial classes including 15 antimicrobials. Utilizing generalized linear mixed models (GLMMs) and co-occurrence analyses, we further explored the multiple-drug-resistance (MDR) combinations and dynamic patterns of E. coli over 10 years in two food animals. RESULTS: Total of 863 MDR isolates were found among 945 collected E. coli isolates, 337 from poultry and 608 from swine. Both isolates exhibited high resistant rates (> 70%) to tetracyclines, phenicols, sulfonamides, penicillins, and aminoglycosides (only in swine). The resistant rates of swine isolates to penicillins, aminoglycosides, tetracyclines, phenicols, and polymyxins were significantly higher than those of poultry isolates, whereas resistance to fluoroquinolones was reversed. Resistance to polymyxins decreased similarly in swine (42.4% in 2009 to 0.0% in 2021) and poultry isolates (from 16.5% to 0.0%). However, resistance to other seven antimicrobial classes (excluding carbapenems and penicillins) declined dramatically in swine isolates, particularly fluoroquinolones (from 80.5% to 14.4%), and tendencies of resistance to the seven classes showed markedly divergent patterns in poultry isolates. Using Poisson GLMMs, the AMR carriage since 2016 was significantly lower than that of 2009 (odds ratio < 1), indicating a decline in the risk of MDR emergence. Furthermore, despite the highly diverse MDR profiles, co-occurrence analysis identified two prominent MDR clusters of penicillins-phenicols-fluoroquinolones in poultry and aminoglycosides-tetracyclines-sulfonamides-phenicols in swine. CONCLUSIONS: Our study uncovered vastly distinct AMR patterns and dynamic tendencies of poultry and swine E. coli isolates from Chongming. Meanwhile, Chongming's AMR status has ameliorated, as indicated by the decline in antimicrobials prevalence (particularly in swine), lower likelihood of MDR emergence and low carbapenem-, cephalosporin-, and polymyxin resistance. Importantly, this surveillance results are the vital basis for future policy development in Chongming and Shanghai.


Assuntos
Anti-Infecciosos , Infecções por Escherichia coli , Aminoglicosídeos , Animais , Antibacterianos/farmacologia , Carbapenêmicos , Cefalosporinas , China/epidemiologia , Farmacorresistência Bacteriana , Escherichia coli , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/veterinária , Fluoroquinolonas , Testes de Sensibilidade Microbiana , Penicilinas , Polimixinas , Aves Domésticas , Sulfonamidas , Suínos , Tetraciclinas
4.
Curr Microbiol ; 79(11): 326, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36125553

RESUMO

This study aimed to investigate the prevalence of Methicillin- and Vancomycin-Resistant Staphylococcus aureus (MRSA, VRSA) and Vancomycin-Resistant Enterococcus (VRE) of hospital food samples in Mashhad, Iran. A total of 357 hospital food samples were collected from 13 hospitals. Enterococcus spp. and Staphylococcus aureus were identified using conventional cultural techniques following genotypic confirmation by PCR. The antibiotic resistance patterns of MRSA, VRSA, and VRE strains were analyzed using the disk diffusion methods. The prevalence of S. aureus and MRSA were 24.37% (87/357) and 22.98% (20.87), respectively. In addition, the vanB gene involved in vancomycin resistance was detected in 1.14% of the S. aureus strains. Enterococci and VRE had a prevalence of 15.4% (55/357) and 21.81% (12/55), respectively. Meat, chicken barbecues, and salad were the most commonly contaminated samples with S. aureus, MRSA, Enterococci, and VRE. PCR detected two vancomycin resistance genes, including vanA (1.81%, 1.55) and vanC2 (20%, 11.55) genes. MRSA strains revealed the highest resistance against penicillin, erythromycin, clindamycin, azithromycin, tetracycline, and gentamicin. The VRSA isolates were resistant to penicillin, ampicillin, oxacillin, cefoxitin, clindamycin, erythromycin, gentamicin, and trimethoprim-sulfamethoxazole. Furthermore, VRE isolates exhibited the highest resistance against quinupristin-dalfopristin, erythromycin, and tetracycline. The results of this study indicated that hospital foods might act as a reservoir of Enterococci spp. and S. aureus strains, which can transfer antibiotic resistance. Moreover, multidrug resistance (MDR) in some MRSA, VRSA, and VRE isolates represents a serious threat to susceptible persons in hospitals.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Enterococos Resistentes à Vancomicina , Ampicilina , Antibacterianos/farmacologia , Azitromicina , Cefoxitina , Clindamicina , Farmacorresistência Bacteriana/genética , Gentamicinas , Hospitais , Meticilina , Testes de Sensibilidade Microbiana , Oxacilina , Prevalência , Staphylococcus aureus , Tetraciclinas , Combinação Trimetoprima e Sulfametoxazol , Vancomicina/farmacologia , Enterococos Resistentes à Vancomicina/genética , Staphylococcus aureus Resistente à Vancomicina
5.
J Clin Invest ; 132(17)2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36047498

RESUMO

Several classes of antibiotics have long been known for protective properties that cannot be explained through their direct antimicrobial effects. However, the molecular bases of these beneficial roles have been elusive. In this issue of the JCI, Mottis et al. report that tetracyclines induced disease tolerance against influenza virus infection, expanding their protection potential beyond resistance and disease tolerance against bacterial infections. The authors dissociated tetracycline's disease-resistance properties from its disease-tolerance properties by identifying potent tetracycline derivatives with minimal antimicrobial activity but increased capacity to induce an adaptive mitochondrial stress response that initiated disease tolerance mechanisms. These findings have potential clinical applications in viral infections.


Assuntos
Anti-Infecciosos , Infecções Bacterianas , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Humanos , Testes de Sensibilidade Microbiana , Tetraciclina/farmacologia , Tetraciclinas/farmacologia , Tetraciclinas/uso terapêutico
6.
Int J Med Microbiol ; 312(6): 151559, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35961233

RESUMO

INTRODUCTION: The emergence of multidrug resistance in Bacteroides fragilis, especially the phylogenetic lineage carrying the carbapenemase gene cfiA, represents an increasing threat to human health. However, knowledge on the diversity of the multidrug-resistant strains and the genetic elements carrying the antibiotic resistance genes (ARGs) remains limited. AIM: The objective of the study was to describe the resistome in cfiA-positive B. fragilis. METHODS: A collection of cfiA-positive B. fragilis from diverse human (8 bacteremias, 15 wound infections) and animal (2 chickens, 2 pigs, 6 dogs, 3 cats) sources in Hong Kong, 2015-2017 was analysed by whole genome sequencing. RESULTS: In the 36 isolates, 13 distinct ARGs (total number 83, median 2, range 0-7 per isolate) other than cfiA were detected. ARGs encoding resistance to aminoglycosides, ß-lactams, macrolides, sulphonamides and tetracyclines were carried by CTn341-like, CTnHyb-like, Tn5220-like, Tn4555-like and Tn613-like transposons and were detected in phylogenetically diverse isolates of different host sources. Only few ARGs encoding resistance to metronidazole and tetracyclines were localized on plasmids. In two chicken isolates, a novel transposon (designated as Tn6994) was found to be involved in the dissemination of multiple ARGs mediating resistance to multiple antibiotics, including metronidazole and linezolid that are critically important for treatment of anaerobic infections. In mating experiments, Tn6994 and the associated phenotypic resistance could be transferred to Bacteroides nordii recipient. CONCLUSION: This study illustrates the importance of transposons in the dissemination of ARGs in the cfiA-positive division of B. fragilis. One Health approach is necessary to track the dissemination of ARGs.


Assuntos
Infecções Bacterianas , Infecções por Bacteroides , Aminoglicosídeos , Animais , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Bacteroides fragilis/genética , Galinhas , Cães , Resistência Microbiana a Medicamentos , Humanos , Linezolida , Macrolídeos , Metronidazol , Testes de Sensibilidade Microbiana , Filogenia , Sulfonamidas , Suínos , Tetraciclinas , Sequenciamento Completo do Genoma , beta-Lactamases/genética , beta-Lactamas
7.
Anal Chim Acta ; 1221: 340130, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35934365

RESUMO

In this study, a novel fluorescence sensor for tetracyclines (TCs) detection was designed using WS2 quantum dots (WS2 QDs). WS2 QDs could be quenched by TCs through the inner filter effect (IFE). The limit of detection of this proprosed method is 39 nM, 52 nM, and 28 nM for tetracycline (TC), doxycycline (DC), and oxytetracycline (OTC), respectively. The as-proposed strategy was successfully applied to detect TC in milk samples and human serum samples. The WS2 QDs were highly biocompatible and showed lower toxicity. Moreover, the WS2 QDs was successfully applied to imaging TC in HeLa cells owing to its excellent optical performance and great biocompatibility.


Assuntos
Pontos Quânticos , Antibacterianos/toxicidade , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Limite de Detecção , Pontos Quânticos/toxicidade , Espectrometria de Fluorescência/métodos , Tetraciclinas
8.
Food Microbiol ; 107: 104090, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35953184

RESUMO

This study analyzed the effect of food-related stresses on the expression of antibiotic resistance of starter and protective strains and resistance gene transfer frequency. After exposure to high-pressure processing, acidic and osmotic stress, the expression of genes encoding resistance to aminoglycosides (aac(6')Ie-aph(2″)Ia and aph(3')-IIIa) and/or tetracyclines (tetM) increased. After cold stress, a decrease in the expression level of all tested genes was observed. The results obtained in the gene expression analysis correlated with the results of the phenotype patterns. After acidic and osmotic stresses, a significant increase in the frequency of each gene transfer was observed. To the best of the authors' knowledge, this is the first study focused on changes in antibiotic resistance associated with a stress response among starter and protective strains. The results suggest that the physicochemical factors prevailing during food production and storage may affect the phenotype of antibiotic resistance and the level of expression of antibiotic resistance genes among microorganisms. As a result, they can contribute to the spread of antibiotic resistance. This points to the need to verify strains used in the food industry for their antibiotic resistance to prevent them from becoming a reservoir for antibiotic resistance genes.


Assuntos
Aminoglicosídeos , Tetraciclinas , Aminoglicosídeos/farmacologia , Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Resistência Microbiana a Medicamentos , Testes de Sensibilidade Microbiana , Pressão Osmótica , Tetraciclinas/farmacologia
9.
Front Cell Infect Microbiol ; 12: 964283, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35982779

RESUMO

The occurrence of antibiotics and potential health risk of 300 cultured fish samples from 19 provinces in China were investigated. The levels of 28 antibiotics (15 fluoroquinolones, 4 tetracyclines, 8 macrolides and rifampin) in 8 fish species were measured through liquid chromatography electrospray tandem mass spectrometry. As a result, 10 antibiotics were detected with an overall detection frequency of 24.3%, and the individual detection frequency of antibiotics ranged from 0.33 to 16.7%. The extremely high concentrations (above 100 µg/kg) of doxycycline and erythromycin were found in the samples. Antibiotics with high detection frequency was noticed in largemouth bass (41.2%), followed by snakehead (34.4%) and bream (31.2%). Specifically, Heilongjiang, Xinjiang, Qinghai and Gansu presented high detection frequency values of more than 60%. Moreover, the highest mean concentration was observed in Shandong, and the concentration covered from 34.8 µg/kg to 410 µg/kg. Despite the high detection frequency and levels of antibiotics were found in samples, ingestion of cultured fish was not significantly related to human health risks in China, according to the calculated estimated daily intakes and hazard quotients. These results provided us the actual levels of antibiotics in cultured fish and human health risk assessment of consuming fishery products.


Assuntos
Antibacterianos , Poluentes Químicos da Água , Animais , Antibacterianos/análise , China , Peixes , Fluoroquinolonas , Humanos , Medição de Risco , Tetraciclinas/análise , Poluentes Químicos da Água/análise
10.
Antimicrob Agents Chemother ; 66(9): e0068922, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-35943269

RESUMO

Nontuberculous mycobacteria (NTM) infections are increasing worldwide. Mycobacterium avium complex (MAC) and the M. abscessus species are the most commonly cultured NTM and treatment options are limited, especially for the M. abscessus species. In this study, the in vitro activity of eravacycline, a new tetracycline derivative, was tested against 110 clinical isolates of NTM. MIC testing was performed as recommended by the Clinical and Laboratory Standards Institute against 60 isolates of rapidly growing mycobacteria (RGM), of which ~70% were tetracycline resistant. These included M. abscessus subsp. abscessus (8 isolates), M. abscessus subsp. massiliense (5), M. chelonae (10), M. immunogenum (3), M. fortuitum group (20) including 12 doxycycline-resistant isolates, and M. mucogenicum group (10) including three doxycycline-resistant isolates. Due to trailing, eravacycline MICs were read at 80% and 100% inhibition. Eravacycline was active against all RGM species, with MIC50 ranges of ≤0.015 to 0.5 and ≤0.015 to 0.12 µg/mL for 100% and 80% inhibition, respectively. For M. abscessus subsp. abscessus, MIC50 values were 0.12 and 0.03 µg/mL with 100% and 80% inhibition, respectively. MICs for tigecycline were generally within 1 to 2 dilutions of the 100%-inhibition eravacycline MIC values. Fifty isolates of slowly growing mycobacteria (SGM) species, including 16 isolates of MAC, were also tested. While there was no trailing observed in most SGM, the eravacycline MICs were higher (MIC range of >8 µg/mL), except for M. kansasii and M. marinum which had MIC50 values of 1 µg/mL. This study supports further evaluation of eravacycline, including clinical trials for the development of RGM treatment regimens, especially for M. abscessus.


Assuntos
Infecções por Mycobacterium não Tuberculosas , Mycobacterium , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Doxiciclina/farmacologia , Doxiciclina/uso terapêutico , Humanos , Testes de Sensibilidade Microbiana , Infecções por Mycobacterium não Tuberculosas/tratamento farmacológico , Infecções por Mycobacterium não Tuberculosas/microbiologia , Complexo Mycobacterium avium , Micobactérias não Tuberculosas , Tetraciclina/uso terapêutico , Tetraciclinas/farmacologia , Tetraciclinas/uso terapêutico , Tigeciclina/farmacologia , Tigeciclina/uso terapêutico
11.
Antimicrob Agents Chemother ; 66(9): e0068722, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-35976006

RESUMO

The 12-month therapy duration for the treatment of Mycobacterium kansasii pulmonary disease calls for more efficacious drugs for better treatment outcomes and to shorten the therapy duration. We performed (i) omadacycline MIC with M. kansasii ATCC 12478 strain and 21 clinical isolates, (ii) dose-response study in the hollow fiber system model of M. kansasii (HFS-Mkn) with six human equivalent omadacycline daily doses to determine the optimal drug exposure for the maximal kill, and (iii) a second HFS-Mkn study to determine the efficacy of omadacycline (300 mg/day) plus moxifloxacin (600 mg/day) plus tedizolid (200 mg/day) combination regimen with standard regimen as comparator. GraphPad Prism was used for data analysis and graphing. MIC of the reference strain was 4 mg/L but ranged from 8 to 32 mg/L among the 21 clinical isolates. In the HFS-Mkn, the exposure required for 50% of the maximal effect (EC50) was an omadacycline area under the concentration-time curve to MIC (AUC0-24/MIC) ratio of 1.95. The optimal exposure was an AUC0-24/MIC of 3.05, which could be achieved with 300 mg/day clinical dose. The omadacycline-moxifloxacin-tedizolid combination sterilized the HFS-Mkn in 14 days with a linear-regression based kill rate of -0.309 ± 0.044 log10 CFU/mL/day compared to the kill rate of -0.084 ± 0.036log10 CFU/mL/day with the standard regimen or 3.7-times faster. Omadacycline has efficacy against M. kansasii and could be used at 300 mg/day in combination with moxifloxacin and tedizolid for the treatment of M. kansasii pulmonary diseases with the potential to shorten the currently recommended 12-month therapy duration.


Assuntos
Pneumopatias , Mycobacterium kansasii , Antibacterianos/uso terapêutico , Humanos , Pneumopatias/microbiologia , Testes de Sensibilidade Microbiana , Moxifloxacina/farmacologia , Moxifloxacina/uso terapêutico , Tetraciclinas
12.
J Oleo Sci ; 71(9): 1363-1374, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35965088

RESUMO

Probiotics frontier in depressing the clinical bacterial pathogens to avoid multidrug resistance phenomenon. The present study aimed to determine the antibacterial efficiency of chitosan encapsulated probiotics isolated from buffalo milk samples against clinical bacterial pathogens. The Agar well method was used for antibacterial activity. Lactococcus lactis (A) and Lactobacillus curvattus (B) were isolated from fresh buffalo milk samples, identified via culturing media, Gram's staining, biochemical tests, and antibiogram analysis. Encapsulation of probiotics was carried out using chitosan and was characterized via a scanning electron microscope. Antibiogram analysis elicit that L. lactis culture (A1) was highly sensitive to chloramphenicol (17.66±0.47 mm), tobramycin (15.33±0.47 mm), and ciprofloxacin (12.33±0.47 mm) and resistant against tetracycline, Penicillin G, Erythromycin, Amoxycillin, Ceftriaxone, Cephalothin, and Cephradine, while L. curvattus culture (B1) was affected by Ceftriaxone (18.67±0.47 mm), Amoxycillin (14.33±0.94 mm), Cephalothin (13.67±0.47 mm), Erythromycin (13.33±0.47 mm), Penicillin G (12.67±0.47 mm), Cephradine (10.33±0.47 mm), and Chloramphenicol (9.67±0.47 mm) and resistant against tetracycline, Tobramycin, and Ciprofloxacin. Antibacterial efficacy of non-encapsulated probiotic cultures was significant and maximum inhibition of bacterial were recorded compared to their cellular components. SEM of encapsulated probiotics revealed that they were successfully covered with a chitosan protective layer and could be effective as bio-preservatives due to being slowly released at the target site. The current study concluded that L. lactis, L. curvattus, and their cellular components have a significant bactericidal effect against infectious pathogens and could be used as a potential therapeutic drug against infectious diseases.


Assuntos
Quitosana , Lactococcus lactis , Probióticos , Amoxicilina , Animais , Antibacterianos/farmacologia , Búfalos , Ceftriaxona , Cefalotina , Cefradina , Quitosana/farmacologia , Cloranfenicol , Ciprofloxacina , Eritromicina , Lactobacillus/fisiologia , Lactococcus lactis/química , Lactococcus lactis/fisiologia , Probióticos/farmacologia , Tetraciclinas , Tobramicina
13.
ACS Biomater Sci Eng ; 8(9): 3957-3968, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-35976991

RESUMO

Tetracyclines are well-known antibiotics and widely used against a variety of bacterial infections. Their monitoring and detection have been an important issue. To this end, a vast number of methods have been developed; fluorescence sensing is one of the most reported. However, most of the reported sensors are made from transition metals with sophisticated multiprocesses; polymers are hardly seen for this purpose, particularly biocompatible ones. Herein, an aqueous solution of poly(ethylene glycol) (PEG), well known for being biocompatible, is shown to emit under excitation of 280 nm, while the solutions of selected tetracyclines, namely, doxycycline (DOX) and tetracycline (TC), are non-emissive under the same conditions. In the binary solutions of PEG-DOX or PEG-TC, PEG emission is sharply quenched with high sensitivity and selectivity. PEG was then used as a sensor for DOX and TC detections in water with high performance compared to reported studies. The same tests were also done by DOX spiking in milk and tap water, demonstrating that DOX was practically fully recovered. The quenching mechanism was ascribed to the interaction between the O atoms of PEG in clusters and specific heteroatom groups on tetracycline molecules through hydrogen bonding, elucidated from FTIR and NMR analyses. Therefore, this work provides a novel, fully green, easy to operate, low cost, and reliable protocol for tetracycline monitoring and detection and opens new potential application for PEG.


Assuntos
Polietilenoglicóis , Tetraciclinas , Antibacterianos , Polietilenoglicóis/química , Tetraciclina/análise , Tetraciclinas/análise , Água/química
14.
New Microbiol ; 45(3): 210-212, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35920876

RESUMO

We evaluated the in vitro activity of eravacycline and cefoperazone/sulbactam against 42 XDR and 58 PDR Acinetobacter baumannii isolates from blood and bronchoalveolar infections. The minimum and maximum MICs for eravacycline were 0.125 and 4 mg/L, respectively. The MIC50 was 2 mg/L and the MIC90 was 3 mg/L. The minimum and maximum MICs for cefoperazone/sulbactam were 24 and >256 mg/L, respectively. The MIC50 and MIC90 were both >256 mg/L. These novel agents were not adequate for the treatment of A. baumannii infections in our hospital and we recommend that mi- crobiology laboratories perform their own evaluations before including them in clinical practice.


Assuntos
Infecções por Acinetobacter , Acinetobacter baumannii , Infecções por Acinetobacter/tratamento farmacológico , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Cefoperazona/farmacologia , Cefoperazona/uso terapêutico , Farmacorresistência Bacteriana Múltipla , Grécia , Humanos , Testes de Sensibilidade Microbiana , Sulbactam/farmacologia , Sulbactam/uso terapêutico , Centros de Atenção Terciária , Tetraciclinas
15.
Huan Jing Ke Xue ; 43(8): 4166-4178, 2022 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-35971714

RESUMO

Antibiotics and antibiotic resistance genes (ARGs) in livestock and poultry manure pose potential ecological risks. In order to understand the distribution characteristic of antibiotics and ARGs in manure and surrounding soils of poultry farms in Ningxia, the poultry manure and relative soil samples were collected from 12 layers of different poultry breeding farms. The compositions of antibiotics and ARGs in the samples were analyzed using UPLC-MS/MS and HT-qPCR. The results showed that:① tetracycline, aminoglycoside, and sulfonamide were the dominant antibiotics in poultry manure. The types and contents of antibiotics in poultry manure were different in different breeding periods. There were more types of antibiotics in the brooding period, the average content was high, and the initial stage showed the opposite trend. ② A small amount of antibiotics was detected in the surrounding soil only 20 m away from the poultry farm, and the poultry farm had little effect on the distribution of antibiotics in the surrounding soil. The content of quinolone in the soils with poultry manure application was significantly higher than that in the control and surrounding soil. ③ We detected 132-168 ARGs in poultry manure, and the number of aminoglycosides and tetracycline was higher. The relative abundance of ARGs in the rearing period was highest, and the initial stage showed the opposite trend. The total relative abundance of ARGs in the brooding period was highest, but the terminal period showed the opposite. There were 110 ARGs in poultry manure during all breeding periods. ④ There were 23-105 ARGs in the soils, and the number of aminoglycoside was highest, followed by multidrug ARGs. The poultry farm had a great effect on the number and relative abundance of ARGs in the surrounding soil. For example, the number and relative abundance of ARGs in the surrounding soil of poultry farms gradually decreased with the increase in the distance from the poultry farms. The number and relative abundance of ARGs in the soil with applied poultry manure were significantly increased; however, these values were lower than those in the soil 20 m away from the poultry farm. ⑤ ß-lactamases, aminoglycosides, and macrolide lincosamide-streptogramin B (MLSB) ARGs were all at risk of horizontal movement in manure, and chloramphenicol ARGs were at risk of horizontal movement in soil. Correlation analysis showed that the relative abundance of aminoglycoside, tetracycline, sulfonamide, ß-lactamase, and MLSB were not significantly correlated with their contents. ⑥ Different types of ARGs had related co-occurrence phenomena, such as the positive correlation between the relative abundance of ARGs in poultry manure, and aminoglycoside and ß-lactamases, MGEs, multidrugs and vancomycins. The relative abundances of ARGs in soil, aminoglycoside and tetracyclines, vancomycins, sulfonamides, and MLSBs; tetracyclines and MLSBs; etc., all showed a significant positive correlation. In short, the co-occurrence among the relative abundance of ARGs in soil was significantly stronger than that in poultry manure. These results could provide the theoretical basis for the site selection of poultry farms, the selection of antibiotic types and dosages for large-scale breeding of laying hens, and the application of poultry manure.


Assuntos
Esterco , Solo , Aminoglicosídeos/farmacologia , Animais , Antibacterianos/farmacologia , Galinhas/genética , Cromatografia Líquida , Resistência Microbiana a Medicamentos/genética , Fazendas , Feminino , Genes Bacterianos , Macrolídeos/farmacologia , Esterco/análise , Aves Domésticas/genética , Microbiologia do Solo , Sulfonamidas/farmacologia , Espectrometria de Massas em Tandem , Tetraciclina/farmacologia , Tetraciclinas/farmacologia , beta-Lactamases/genética
16.
PLoS One ; 17(8): e0272763, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35980971

RESUMO

BACKGROUND: Antimicrobial residues (ABs) in foods contribute to the development of antimicrobial resistance, which is becoming a major public health concern around the world. Understanding food production practices concerning antimicrobial use and consumer awareness on the possibility of ABs in foods is necessary for developing mitigation strategies. Therefore, this study was conducted to assess the production practices and awareness among eggs and Chinese cabbage consumers in Dodoma city. METHODS: A cross-sectional study was conducted using a structured questionnaire and checklist to collect data on awareness and production practices from 420 consumers, 30 chicken egg farmers, and 30 Chinese cabbage farmers in eight city wards. FINDINGS: About 42% of consumers of eggs and Chinese cabbages were not aware of the likelihood of antimicrobial residues in these foods. The awareness was significantly influenced by the consumer's educational level (p = 0.001) and geographical location (p = 0.045), with educated and urban consumers being 7.7 and 1.6 times more informed than their uneducated and peri-urban counterparts. Furthermore, the majority of chicken farmers raised more layers than broilers, and they all used antimicrobial drugs, mainly tetracycline and its variants, to treat and prevent diseases. The findings also revealed that many vegetable producers utilized wastes from animals treated with antimicrobials as manure to amend the soil. CONCLUSION: In a view of the findings, a considerable number of egg and Chinese cabbage consumers in Dodoma city were unaware on the likelihood of antimicrobial residues in these foods from their production practices. All chicken farmers utilized antimicrobial drugs mainly tetracycline and its derivatives, for the treatment and prevention of diseases while vegetable producers used wastes from animals treated with antimicrobials as soil manure. Therefore, initiatives to inform farmers and consumers about the possibility of antimicrobial residues in these foods and their related public health risks upon long-term consumption are strongly advised.


Assuntos
Anti-Infecciosos , Brassica , Animais , Antibacterianos/uso terapêutico , Anti-Infecciosos/uso terapêutico , Galinhas , China , Estudos Transversais , Fazendeiros , Humanos , Esterco , Solo , Tanzânia , Tetraciclinas
17.
Mikrochim Acta ; 189(9): 352, 2022 08 26.
Artigo em Inglês | MEDLINE | ID: mdl-36008501

RESUMO

A ratiometric fluorescence probe is proposed for sensitive and visual detection of tetracyclinee (TC) based on cascade fluorescence signal amplification induced by bovine serum albumin-stabilized copper nanoclusters (BSA-CuNCs) and yttrium ions (Y3+). TC can combine with Y3+ to form the complex (TC-Y3+) to enhance the fluorescence of TC at 515 nm. Then, positively charged TC-Y3+ and negatively charged BSA-CuNCs was bonded together by electrostatic interactions to achieve the fluorescence resonance energy transfer (FRET) process. With the increase of TC concentration, the fluorescence intensity of TC-Y3+ at 515 nm (F515) gradually increased; meanwhile, the fluorescence intensity of BSA-CuNCs at 405 nm (F405) decreased gradually. The ratio of F515 and F405 was used for the quantitative determination of TC. The linear range of the constructed fluorescent probe is 1.0 to 60.0 µM, and the limit of detection is 0.22 µM. The method was successfully applied to the determination of TC in spiked milk with recoveries ranging from 94.3 to 112%. Furthermore, the color of this platform can be observed from dark violet to bright green under the UV lamp. Since the response time of the reaction is less than 10 s, an intelligent sensing platform based on the use of the smartphone as image acquisition equipment was also established to realize rapid on-site and portable detection of TC through the colorimetric recognition application.


Assuntos
Cobre , Corantes Fluorescentes , Antibacterianos , Soroalbumina Bovina , Espectrometria de Fluorescência/métodos , Tetraciclinas
18.
J Antibiot (Tokyo) ; 75(9): 498-508, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35896611

RESUMO

Eravacycline (Erava) is a synthetic fluorocycline with potent antimicrobial activity against a wide range of Gram-positive bacteria. This study aimed to investigate the in vitro antimicrobial activity and resistance mechanism of Erava in clinical E. faecium isolates from China. Erava minimum inhibitory concentrations (MICs) against clinical E. faecium isolates-including those resistant to linezolid (LZD) or harboring the tetracycline (Tet) resistance genes was ≤0.25 mg l-1. Moreover, our data indicated that clinical isolates of E. faecium with Erava MIC 0.25 mg l-1 were predominantly shown to belong to Sequence-type 78 (ST78) and ST80. The prevalence of Erava heteroresistance in clinical E. faecium strain was 2.46% (3/122). The increased Erava MIC values of heteroresistance-derived E. faecium clones could be significantly reduced by efflux pump inhibitors (EPIs). Furthermore, comparative proteomics results showed that efflux pumps lmrA, mdlA, and mdlB contributed significantly to the acquisition of Erava resistance in E. faecium. In addition, a genetic mutation in 16 S rRNA (G190A) were detected in resistant E. faecium isolates induced by Erava. In summary, Erava exhibits potent in vitro antimicrobial activity against E. faecium, but mutation of Tet target sites and elevated expression of efflux pumps under Erava selection results in Erava resistance.


Assuntos
Enterococcus faecium , Infecções por Bactérias Gram-Positivas , Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Enterococcus faecalis , Humanos , Testes de Sensibilidade Microbiana , Tetraciclinas/farmacologia
19.
J Clin Invest ; 132(17)2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35787521

RESUMO

Mitohormesis defines the increase in fitness mediated by adaptive responses to mild mitochondrial stress. Tetracyclines inhibit not only bacterial but also mitochondrial translation, thus imposing a low level of mitochondrial stress on eukaryotic cells. We demonstrate in cell and germ-free mouse models that tetracyclines induce a mild adaptive mitochondrial stress response (MSR), involving both the ATF4-mediated integrative stress response and type I interferon (IFN) signaling. To overcome the interferences of tetracyclines with the host microbiome, we identify tetracycline derivatives that have minimal antimicrobial activity, yet retain full capacity to induce the MSR, such as the lead compound, 9-tert-butyl doxycycline (9-TB). The MSR induced by doxycycline (Dox) and 9-TB improves survival and disease tolerance against lethal influenza virus (IFV) infection when given preventively. 9-TB, unlike Dox, did not affect the gut microbiome and also showed encouraging results against IFV when given in a therapeutic setting. Tolerance to IFV infection is associated with the induction of genes involved in lung epithelial cell and cilia function, and with downregulation of inflammatory and immune gene sets in lungs, liver, and kidneys. Mitohormesis induced by non-antimicrobial tetracyclines and the ensuing IFN response may dampen excessive inflammation and tissue damage during viral infections, opening innovative therapeutic avenues.


Assuntos
Influenza Humana , Infecções por Orthomyxoviridae , Animais , Antibacterianos , Doxiciclina/farmacologia , Humanos , Influenza Humana/tratamento farmacológico , Camundongos , Tetraciclina , Tetraciclinas/farmacologia
20.
Biomed Pharmacother ; 153: 113334, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35797943

RESUMO

The persistent incidence of high levels of multidrug-resistant (MDR) bacteria seriously endangers global public health. In response to MDR-associated infections, new antibacterial drugs and strategies are particularly needed. Screening to evaluate a potential compound to reverse antibiotic resistance is a good strategy to alleviate this crisis. In this paper, using high-throughput screening methods, we identified that oxyclozanide potentiated tetracycline antibiotics act against MDR bacterial pathogens by promoting intracellular accumulation of tetracycline in resistant bacteria. Furthermore, mechanistic studies demonstrated that oxyclozanide could directly kill bacteria by disrupting bacterial membrane and inducing the overproduction of bacterial reactive oxygen species. Oxyclozanide effectively reduced the production of virulence proteins in S. aureus and neutralized the produced α-hemolysin, thereby effectively alleviating the inflammatory response caused by bacteria. Finally, oxyclozanide significantly reversed tetracycline resistance in animal infection assays. In summary, these results demonstrated the capacity of oxyclozanide as a novel antibiotic adjuvant, antibacterial and anti-virulence multifunctional compound to circumvent MDR bacteria and improve the therapeutic effect of persistent infections caused by MDR bacteria worldwide.


Assuntos
Oxiclozanida , Staphylococcus aureus , Animais , Antibacterianos/farmacologia , Bactérias , Farmacorresistência Bacteriana Múltipla , Testes de Sensibilidade Microbiana , Oxiclozanida/farmacologia , Tetraciclinas/farmacologia
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