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2.
J Am Assoc Lab Anim Sci ; 60(5): 587-591, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34325772

RESUMO

Tinidazole is a second-generation nitroimidazole compound that is used as an antimicrobial to treat anaerobic bacterial and protozoal infections in humans and, less frequently, in veterinary medicine. However, metronidazole, another secondgeneration nitroimidazole, is more commonly used. Nonetheless, tinidazole has proven to be a superior therapy for parasitic infections in humans, particularly in the treatment of giardiasis. Furthermore, in chinchillas, metronidazole has been shown to cause a clinically relevant reduction in food intake after oral administration at published dosages. This study's objective was to evaluate the effect of orally administered tinidazole on food intake in healthy chinchillas. In 2 randomized, placebocontrolled, blinded, crossover studies, tinidazole was evaluated at 2 single high doses (100 mg/kg and 200 mg/kg PO, n = 9) and a repeated dosing schedule at a lower dose (20 mg/kg PO q12h for 10 doses, n = 12). Food intake was measured over 24-h periods before and after drug administration. The single-dose treatment groups both displayed significantly reduced food intake (200 mg/kg: -26 ± 21%; 100 mg/kg: -9 ± 21%, P < 0.01) as compared with the control group during the first 24 h after drug administration. Food intake returned to pretreatment values within 4-5 d. Repeated administration at 20 mg/kg q12h was not associated with any significant changes in food intake. No other adverse effects were noted during this study. Tinidazole administration at single higher doses resulted in an acute self-limiting reduction in food intake. In comparison, repeated administration of lower doses (20 mg/kg PO q12h) had no significant effects on food intake in healthy chinchillas. Therefore, tinidazole may be a more suitable drug for treating Giardia and anaerobic bacterial infections in this species than the more commonly used metronidazole.


Assuntos
Nitroimidazóis , Tinidazol , Animais , Chinchila , Ingestão de Alimentos , Humanos , Metronidazol
3.
BMC Infect Dis ; 21(1): 642, 2021 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-34218802

RESUMO

BACKGROUND: Antibiotic-resistance reduces the efficacy of conventional triple therapy for Helicobacter Pylori infections worldwide, which necessitates using various treatment protocols. We used two protocols, doxycycline-based quadruple regimen and concomitant levofloxacin regimen. The aim was to assess the effectiveness of doxycycline-based quadruple regimen for treating Helicobacter Pylori infections compared with levofloxacin concomitant regimen as empirical first-line therapy based on intention-to-treat (ITT) and per-protocol analyses (PPA) in Syrian population. SETTINGS AND DESIGN: An open-label, randomised, parallel, superiority clinical trial. METHODS: We randomly assigned 78 naïve patients who tested positive for Helicobacter Pylori gastric infection, with a 1:1 ratio to (D-group) which received (bismuth subsalicylate 524 mg four times daily, doxycycline 100 mg, tinidazole 500 mg, and esomeprazole 20 mg, each twice per day for 2 weeks), or (L-group) which received (levofloxacin 500 mg daily, tinidazole 500 mg, amoxicillin 1000 mg, and esomeprazole 20 mg each twice per day for two weeks). We confirmed Helicobacter Pylori eradication by stool antigen test 8 weeks after completing the treatment. RESULTS: Thirty-nine patients were allocated in each group. In the D-group, 38 patients completed the follow-up, 30 patients were cured. While in the L-group, 39 completed the follow-up, 32patients were cured. According to ITT, the eradication rates were 76.92%, and 82.05%, for the D-group and L-group respectively. Odds ratio with 95% confidence interval was 1.371 [0.454-4.146]. According to PPA, the eradication rates were 78.9%, and 82.05% for the D-group and L-group respectively. The odds ratio with 95% confidence interval was 1.219 [0.394-3.774]. We didn't report serious adverse effects. CONCLUSIONS: Levofloxacin concomitant therapy wasn't superior to doxycycline based quadruple therapy. Further researches are required to identify the optimal first-line treatment for Helicobacter-Pylori Infection in the Syrian population. TRIAL REGISTRATION: We registered this study as a standard randomized clinical trial ( Clinicaltrial.gov , identifier- NCT04348786 , date:29-January-2020).


Assuntos
Antibacterianos/uso terapêutico , Doxiciclina/uso terapêutico , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/efeitos dos fármacos , Levofloxacino/uso terapêutico , Adulto , Amoxicilina/uso terapêutico , Bismuto/uso terapêutico , Quimioterapia Combinada , Esomeprazol/uso terapêutico , Fezes/microbiologia , Feminino , Helicobacter pylori/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Compostos Organometálicos/uso terapêutico , Estudos Prospectivos , Salicilatos/uso terapêutico , Síria , Tinidazol/uso terapêutico , Resultado do Tratamento , Adulto Jovem
4.
Mayo Clin Proc ; 96(7): 2005-2007, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34218872
5.
Int J STD AIDS ; 32(1): 89-91, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33121364

RESUMO

Metronidazole desensitization is recommended in patients with trichomoniasis and history of an allergic reaction to metronidazole due to presumed cross reactivity with tinidazole and lack of reliably safe and effective alternative therapies. We report our experiences in a patient with persistent trichomoniasis who failed to complete metronidazole desensitization due to a burning sensation over her whole body and pruritus but was later successfully desensitized to tinidazole without experiencing any adverse effects.


Assuntos
Antitricômonas/farmacologia , Antitricômonas/uso terapêutico , Metronidazol/efeitos adversos , Tinidazol/uso terapêutico , Tricomoníase/tratamento farmacológico , Trichomonas vaginalis/efeitos dos fármacos , Adulto , Resistência a Medicamentos , Feminino , Humanos , Hipersensibilidade , Resultado do Tratamento , Trichomonas vaginalis/isolamento & purificação
6.
Acta Medica (Hradec Kralove) ; 63(4): 183-187, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33355079

RESUMO

BACKGROUND: The aim of this study was comparison the effectiveness of sequential and standard quadruple therapy on eradication of H. pylori infection. METHODS: This clinical trial study was conducted on 160 patients with dyspepsia or gastroduodenal ulcer. Patients were randomly divided into two groups. Group A (standard regimen) received omeprazole, amoxicillin, clarithromycin and bismuth subcitrate for 2 weeks. Group B (sequential regimen) received omeprazole and amoxicillin in 5 days and omeprazole, tinidazole and levofloxacin in 5 days. After the end of treatment regimens, 20 mg omeprazole was administered twice daily for 3 weeks. H. pylori eradication was assessed 2 months after antibiotic treatment via fecal antigen. RESULTS: Frequency of H. pylori eradication in group A and B was observed in 55 (68.8%) and 63 patients (78.8%), respectively. No significant difference was seen between two groups, regarding H. pylori eradication (p = 0.15). The most common side effects in group A, B were bitterness of mouth (63.8%) and nausea (16.2%), respectively (p H. pylori infection, higher rate of H. pylori eradication was seen in group B than group A. Thus, sequential regimen was a more appropriate regimen with fewer complications.


Assuntos
Infecções por Helicobacter/tratamento farmacológico , Adulto , Idoso , Amoxicilina/administração & dosagem , Claritromicina/administração & dosagem , Quimioterapia Combinada , Feminino , Helicobacter pylori , Humanos , Levofloxacino/administração & dosagem , Masculino , Pessoa de Meia-Idade , Omeprazol/administração & dosagem , Compostos Organometálicos/administração & dosagem , Tinidazol/administração & dosagem
8.
Int J Nanomedicine ; 15: 5473-5489, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801701

RESUMO

Introduction: Biofilms protect bacteria from antibiotics and this can produce drug-resistant strains, especially the main pathogen of periodontitis, Porphyromonas gingivalis. Carbon quantum dots with various biomedical properties are considered to have great application potential in antibacterial and anti-biofilm treatment. Methods: Tinidazole carbon quantum dots (TCDs) and metronidazole carbon quantum dots (MCDs) were prepared by a hydrothermal method with the clinical antibacterial drugs tinidazole and metronidazole, respectively. Then, TCDs and MCDs were characterized by transmission electron microscopy, UV-visible spectroscopy, infrared spectroscopy and energy-dispersive spectrometry. The antibacterial effects were also investigated under different conditions. Results: The TCDs and MCDs had uniform sizes. The results of UV-visible and energy-dispersive spectrometry confirmed their important carbon polymerization structures and the activity of the nitro group, which had an evident inhibitory effect on P. gingivalis, but almost no effect on other bacteria, including Escherichia coli, Staphylococcus aureus and Prevotella nigrescens. Importantly, the TCDs could penetrate the biofilms to further effectively inhibit the growth of P. gingivalis under the biofilms. Furthermore, it was found that the antibacterial effect of TCDs lies in its ability to impair toxicity by inhibiting the major virulence factors and related genes involved in the biofilm formation of P. gingivalis, thus affecting the self-assembly of biofilm-related proteins. Conclusion: The findings demonstrate a promising new method for improving the efficiency of periodontitis treatment by penetrating the P. gingivalis biofilm with preparations of nano-level antibacterial drugs.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Porphyromonas gingivalis/efeitos dos fármacos , Pontos Quânticos/química , Animais , Antibacterianos/efeitos adversos , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Carbono/química , Carbono/farmacologia , Escherichia coli/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Humanos , Metronidazol/química , Metronidazol/farmacologia , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Transmissão , Periodontite/microbiologia , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/fisiologia , Coelhos , Espectrofotometria Ultravioleta , Staphylococcus aureus/efeitos dos fármacos , Tinidazol/química , Tinidazol/farmacologia , Fatores de Virulência/antagonistas & inibidores
9.
Mol Med Rep ; 22(2): 1227-1234, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32626984

RESUMO

Helicobacter pylori (Hp) infection is a major cause of gastrointestinal disease. However, the pathogenesis of gastric mucosa injury by Hp has remained elusive. Small non­coding RNA (sRNA) is a type of widespread RNA in prokaryotic organisms and regulates bacterial growth, reproduction and virulence. In the present study, Hp sRNA profiles were generated to reveal the sequences and possible functions of sRNA by bioinformatics analysis. The role of sRNA in tinidazole (TNZ) treatment was also explored. Total sRNAs of HP26695 were sequenced using an Illumina HiSeq2000. Detected Tags were then compared with a known sRNA database to build an sRNA profile. Reverse transcription­quantitative (RT­q)PCR products were sequenced directly and agarose gel electrophoresis was used to identify NAT­67 and 5'ureB­sRNA in HP. Furthermore, HP was treated with TNZ for 6, 12 and 24 h. The bacterial concentration was measured, the expression of NAT­67, 5'ureB­sRNA and ceuE was determined by RT­qPCR and superoxide dismutase (SOD) activity and reactive oxygen species (ROS) production were detected. A total of 163 sRNA tags were predicted in Hp through bioinformatics analysis. Among them, 35 tags were evolutionarily aconserved in different Hp strains. By target prediction, it was indicated that certain candidate sRNAs were associated with bacterial oxidative stress, virulence and chemotaxis. It was also observed that NAT­67 and 5'ureB­sRNA were downregulated in TNZ­treated HP. TNZ treatment inhibited the growth of Hp, which was accompanied by downregulation of ceuE and SOD activity, as well as upregulation of ROS. RNA sequencing and bioinformatics are valuable in predicting the expression profile and function of sRNA in HP. sRNA­targeted genes may be associated with virulence, oxidative stress and chemokines. Downregulation of NAT­67 by TNZ may be involved in Hp oxidative stress regulation, which may comprise one of the mechanisms of the antibacterial effects of TNZ.


Assuntos
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Helicobacter pylori , RNA Bacteriano/genética , Pequeno RNA não Traduzido/genética , Tinidazol/farmacologia , Virulência/genética , Quimiocinas/metabolismo , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/genética , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/genética , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
10.
Parasitology ; 147(13): 1383-1391, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32729451

RESUMO

BACKGROUND: Trichomonas vaginalis is the most common non-viral sexually transmitted infection. 5-Nitroimidazoles [metronidazole (MTZ) and tinidazole (TDZ)] are FDA-approved treatments. To better understand treatment failure, we conducted a systematic review on mechanisms of 5-nitroimidazole resistance. METHODS: PubMed, ScienceDirect and EMBASE databases were searched using keywords Trichomonas vaginalis, trichomoniasis, 5-nitroimidazole, metronidazole, tinidazole and drug resistance. Non-English language articles and articles on other treatments were excluded. RESULTS: The search yielded 606 articles, of which 550 were excluded, leaving 58 articles. Trichomonas vaginalis resistance varies and is higher with MTZ (2.2-9.6%) than TDZ (0-2%). Resistance can be aerobic or anaerobic and is relative rather than absolute. Differential expression of enzymes involved in trichomonad energy production and antioxidant defenses affects 5-nitroimidazole drug activation; reduced expression of pyruvate:ferredoxin oxidoreductase, ferredoxin, nitroreductase, hydrogenase, thioredoxin reductase and flavin reductase are implicated in drug resistance. Trichomonas vaginalis infection with Mycoplasma hominis or T. vaginalis virus has also been associated with resistance. Trichomonas vaginalis has two genotypes, with greater resistance seen in type 2 (vs type 1) populations. DISCUSSION: 5-Nitroimidazole resistance results from differential expression of enzymes involved in energy production or antioxidant defenses, along with genetic mutations in the T. vaginalis genome. Alternative treatments outside of the 5-nitroimidazole class are needed.


Assuntos
Antiprotozoários/farmacologia , Resistência a Medicamentos , Metronidazol/farmacologia , Tinidazol/farmacologia , Trichomonas vaginalis/efeitos dos fármacos
11.
Spectrochim Acta A Mol Biomol Spectrosc ; 239: 118513, 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-32470818

RESUMO

The aim of the presented work is to compare two popular chemometric methods which are partial least squares regression (PLSR) and support vector regression (SVR). The comparison shows their characteristics via application of the suggested methods to analysis of Norfloxacin (NF) and Tinidazole (TZ) with the presence of a potential impurity of Tinidazole; 2-Methyl-5-nitro-1H-imidazole (MNZ). For appropriate analysis, a 3 factor 4 level experimental design was constructed, which results in a training set composed of 16 mixtures which contains different concentrations of the three components; achieving symmetry, rotatability and orthogonality in mixture space. In order to validate the prediction ability of the suggested models, an independent test set consisting of 8 in-space and 8 out-of-space mixtures was used. The presented results show high specificity and accuracy of the mentioned multivariate calibration models for analysis of in-space samples of NF and TZ in presence of (MNZ) using UV spectral data. Statistical comparisons of predictive abilities of proposed models against classical least squares CLS model and against each other was performed; whether for analysis of test set mixtures or dosage form. CLS model showed lower predictive ability compared to other models. Results obtained by SVR model are as accurate as PLSR model, however, optimization and implementation of PLSR is faster and easier, hence PLSR could be of choice for this given case study. The developed chemometric models were validated as directed by ICH strategies. The validated methods were efficiently used for estimation of NF and TZ in pure powders and pharmaceuticals which indicates their suitability for application in quality control examination of both of the drugs.


Assuntos
Norfloxacino , Tinidazol , Calibragem , Análise dos Mínimos Quadrados , Modelos Lineares
12.
J Clin Pharmacol ; 60(9): 1185-1188, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32408379

RESUMO

After the incidental observation of an almost complete resolution of maculopapular eruption in a patient having simultaneously secondary syphilis and trichomonas vaginalis infection, we extended the treatment with tinidazole (500 mg 4 times daily for 7 days) to 10 other early syphilis patients before the start of the conventional penicillin treatment. All patients showed marked improvement of their lesions in a few days. After the introduction of the conventional penicillin regimen, the lesions further improved and VDRL titers declined at least 4-fold within 6 months in all patients. Tinidazole is a 5-nitroimidazole derivative as well as metronidazole but with a longer plasma half-life. It is activated intracellularly by bacterial/parasitic enzymes to a redox cytotoxic intermediate that damages large protein molecules and inhibits repair and transcription of DNA affecting also the cell wall. With this action, tinidazole might also have a synergic action with penicillin and doxycycline, facilitating the entry of such drugs. It is possible that tinidazole has the same bactericidal action on spirochetes other than Borrelia, such as Treponema pallidum, explaining its rapid therapeutic action on the lesions of early syphilis. Whether this action could be confirmed by studies on larger series of patients, tinidazole might be considered in case of allergy to penicillin or other antibiotics usually prescribed in syphilis.


Assuntos
Antibacterianos/administração & dosagem , Antitricômonas/administração & dosagem , Sífilis/tratamento farmacológico , Tinidazol/administração & dosagem , Administração Oral , Adolescente , Adulto , Feminino , Humanos , Injeções Intramusculares , Masculino , Pessoa de Meia-Idade , Penicilina G Benzatina/administração & dosagem , Sífilis/complicações , Vaginite por Trichomonas/tratamento farmacológico , Trichomonas vaginalis/efeitos dos fármacos , Adulto Jovem
13.
Int J STD AIDS ; 31(3): 221-229, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31996095
14.
Am J Obstet Gynecol ; 222(2): 114-122, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31513780

RESUMO

Vaginitis is one of the most common causes of patient visits to gynecologists, primary care providers, and urgent care centers. However, many women leave without a clear diagnosis or experience recurrent symptoms despite treatment. The 3 most common etiologies of vaginitis are trichomonas, bacterial vaginosis, and vulvovaginal candidiasis, which account for an estimated 70% of cases. The remaining 30% may be related to other causes of vaginitis, including atrophic vaginitis, desquamative inflammatory vaginitis, and vaginal erosive disease. The purpose of this review is to describe the noncandidal causes of acute and recurrent vaginitis, with the goal of improving the likelihood of accurate diagnosis as well as efficient and effective therapy. We excluded candidal vaginitis from our review because there was a recently published review on this topic in the Journal. The clinical presentation and evaluation of patients with symptoms of vaginitis can be triaged into 1 of 2 diagnostic pathways: noninflammatory and inflammatory vaginitis. The most common noninflammatory cause is bacterial vaginosis. Features such as irritation, purulent discharge, and the presence of polymorphonuclear neutrophils are more suggestive of an inflammatory process. Trichomoniasis is the most common cause of inflammatory vaginitis. Other well-described forms of inflammatory vaginitis include atrophic vaginitis, desquamative inflammatory vaginitis, and erosive disease. We present a review of the pathogenesis, symptoms, examination findings, diagnostic testing, and treatment for each of these causes of noncandidal vaginitis.


Assuntos
Anti-Infecciosos/uso terapêutico , Vaginite Atrófica/diagnóstico , Candidíase Vulvovaginal/diagnóstico , Vaginite por Trichomonas/diagnóstico , Vaginose Bacteriana/diagnóstico , Administração Intravaginal , Administração Oral , Anti-Inflamatórios/uso terapêutico , Vaginite Atrófica/terapia , Clindamicina/uso terapêutico , Desidroepiandrosterona/uso terapêutico , Diagnóstico Diferencial , Terapia de Reposição de Estrogênios , Estrogênios/uso terapêutico , Feminino , Humanos , Hidrocortisona/uso terapêutico , Inflamação , Líquen Plano/diagnóstico , Líquen Plano/terapia , Metronidazol/análogos & derivados , Metronidazol/uso terapêutico , Penfigoide Mucomembranoso Benigno/diagnóstico , Penfigoide Mucomembranoso Benigno/terapia , Penfigoide Bolhoso/diagnóstico , Penfigoide Bolhoso/terapia , Pênfigo/diagnóstico , Pênfigo/terapia , Tamoxifeno/análogos & derivados , Tamoxifeno/uso terapêutico , Tinidazol/uso terapêutico , Vaginite por Trichomonas/terapia , Vaginite/diagnóstico , Vaginite/terapia , Vaginose Bacteriana/terapia
15.
J Gastroenterol Hepatol ; 35(4): 617-623, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31758571

RESUMO

BACKGROUND AND AIM: There is little published research to examine the best approach to the management of Helicobacter pylori in Myanmar. This study aimed to determine the relative efficacy and tolerability of sequential eradication therapy compared to Myanmar's current recommendation of a concomitant four drug regimen. METHODS: Patients were screened for H. pylori using monoclonal Stool Antigen Testing (SAT). Those testing positive were randomized 1:1 to receive receive Myanmar's first-line regimen of 14 days of concomitant rabeprazole, clarithromycin, amoxycillin and tinidazole (140 pills, cost US$23) or 10 days of sequential rabeprazole, clarithromycin, amoxycillin and tinidazole (60 pills, cost US$10). Adherence and adverse effects were recorded, and the efficacy of the regimens assessed with repeat SAT. RESULTS: Of the 1011 patients screened for H. pylori infection, 313 (31%) tested positive. There was no statistical difference in the cure rates of the two regimens in either intention-to-treat: 128/157 (82%; 95% confidence interval (CI): 75-87%) receiving sequential therapy versus 123/156 (79%; 95% CI: 72-85%) receiving concomitant therapy (P = 0.55) or per-protocol analysis: 125/131 (95%; 95% CI: 90-98) receiving sequential therapy versus 121/130 (93%; 95% CI: 87-96) receiving concomitant therapy (P = 0.42). Side effects of therapy were reported in 54/157 (47%) patients taking sequential therapy compared with 62/156 (53%) taking concomitant therapy, but this difference did not reach statistical significance (P = 0.33). CONCLUSIONS: In this high-burden, resource-poor setting, less expensive sequential therapy was as effective and as well tolerated as the currently recommended concomitant four drug regimen for eradication of H. pylori.


Assuntos
Amoxicilina/administração & dosagem , Claritromicina/administração & dosagem , Gastrite/tratamento farmacológico , Gastrite/microbiologia , Infecções por Helicobacter , Helicobacter pylori , Rabeprazol/administração & dosagem , Tinidazol/administração & dosagem , Amoxicilina/efeitos adversos , Amoxicilina/economia , Claritromicina/efeitos adversos , Claritromicina/economia , Custos de Medicamentos , Quimioterapia Combinada/economia , Mianmar , Rabeprazol/efeitos adversos , Rabeprazol/economia , Tinidazol/efeitos adversos , Tinidazol/economia , Resultado do Tratamento
16.
J Sep Sci ; 43(6): 1195-1202, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31860164

RESUMO

In this study, an eco-friendly fast simple method was developed for simultaneous determination of norfloxacin and tinidazole based on thin-layer chromatography and image-processing analysis. The binary mixture was separated using reversed phase - thin layer chromatography plates and 30% trifluoroacetic acid only as mobile phase. Mobile phase composition was optimized using Taguchi orthogonal array and Derringer's desirability function. The plates were viewed under UV lamp and photographed by iPhone camera followed by image processing with Fiji software using integrated density as the measured response. As decreasing illumination increases the sensitivity of the method, this method was applied on two different ranges for each drug. The first one was 0.6-6.0 and 0.9-9.0 µg/spot for norfloxacin and tinidazole, respectively measured on the original image with normal illumination. The second one was measured after decreasing the illumination of the captured images at 0.06-0.60 and 0.09-0.90 µg/spot for norfloxacin and tinidazole, respectively. The proposed method was successfully applied for the determination of both drugs in tablets dosage form without interference from the commonly encountered excipients. Analytical Eco-Scale was used to evaluate the greenness profile of the proposed method and it was found to be excellent green analytical method.


Assuntos
Aplicativos Móveis , Norfloxacino/análise , Tinidazol/análise , Cromatografia em Camada Delgada , Ácido Trifluoracético/química
17.
Gac Med Mex ; 155(Suppl 1): S22-S27, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31638607

RESUMO

Introduction: In Mexico, seroprevalence of Entamoeba histolytica is 8.4%. The intestinal amebiasis in patients with acute leukemia of novo, after the start of chemotherapy (CT) in the Hematology Service of the CMN 20 de Noviembre is 12%, even if patients show a negative baseline coprological test. Objective: To find out if the administration of tinidazole, in patients with acute leukemia and negative coprological test, at the beginning of the CT, decreases the incidence of amoebic colitis during the induction to remission. Method: Prospective and not comparative study. Patients with de novo diagnosis of acute leukemia who initiate induction and initial coprological CT. Tinidazole was indicated, 2 g/day for 5 days in the first week of CT started. They were monitored until the induction was concluded and hematopoietic recovery started. Results: 38 patients, 15 women and 23 men with a mean age of 44 years (16-72), with acute lymphoblastic leukemia 19, myeloblastic 16 and promyelocytic 3. Cases without and with intestinal amebiasis were 35 and 3, respectively. Patients with amebiasis only received tinidazole for 3 days and it was given 2 days after the CT started. Conclusion: Tinidazole, in patients with acute de novo leukemia who initiate induction CT, is effective in the prevention of intestinal amebiasis, during the induction stage, if administered at 2 g/day, for five days, starting on day 1 of the CT.


Assuntos
Amebicidas/uso terapêutico , Disenteria Amebiana/prevenção & controle , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Promielocítica Aguda/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Tinidazol/uso terapêutico , Adolescente , Adulto , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Disenteria Amebiana/parasitologia , Feminino , Humanos , Quimioterapia de Indução/métodos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Adulto Jovem
18.
Mater Sci Eng C Mater Biol Appl ; 105: 110017, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546382

RESUMO

Hydroxyapatite (HAp) is a highly promising material as a drug carrier. The solubility, osteoinductivity, antibacterial properties and drug loading efficiency of HAp can be further enhanced by Zn doping. In this study, we carried out first-principles and molecular dynamics (MD) simulations to investigate the influence of Zn doping on the crystal structure and adsorption capacity of macromolecular drugs on HAp. Our results showed that the binding energy of doxorubicin (DOX) on HAp is significantly increased in consequence of Zn-doping. Moreover, the interaction between surface Ca ions and carbonyl-O mostly contributed to the adsorption. The binding energy of tinidazole on HAp was much lower than that observed for DOX. The number of active "O" atoms in the drug and binding stability were positively correlated. These simulations provide important insight into the understanding of drug adsorption on HAp or ion-doped HAp.


Assuntos
Doxorrubicina/química , Durapatita/química , Simulação de Dinâmica Molecular , Tinidazol/química , Zinco/química , Adsorção , Doxorrubicina/farmacologia , Conformação Molecular , Eletricidade Estática , Termodinâmica , Tinidazol/farmacologia
19.
Infez Med ; 27(3): 336-339, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31545780

RESUMO

Caused by the protozoan Giardia lamblia, giardiasis is one of the most common parasitic diarrheal infections affecting humans. Although a variety of antigiardial drugs are available to treat infections in humans, failure of conventional treatment with nitroimidazoles for giardiasis has been increasingly reported. We describe the follow-up of a patient with recurrent giardiasis refractory to nitroimidazoles. Despite the different therapies received, the symptomatology and parasitic forms of G. lamblia persisted in the patient. There is no standard treatment regimen for giardiasis refractory to nitroimidazoles. When treatment failure is confirmed, it is necessary to switch to second-line regimens.


Assuntos
Antiprotozoários/uso terapêutico , Giardia lamblia , Giardíase/tratamento farmacológico , Nitroimidazóis/uso terapêutico , Adulto , Albendazol/uso terapêutico , Antiprotozoários/administração & dosagem , Furazolidona/uso terapêutico , Giardia lamblia/efeitos dos fármacos , Humanos , Masculino , Metronidazol/administração & dosagem , Metronidazol/análogos & derivados , Metronidazol/uso terapêutico , Tiazóis/uso terapêutico , Tinidazol/uso terapêutico , Falha de Tratamento
20.
Eur Rev Med Pharmacol Sci ; 23(3 Suppl): 104-109, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31389581

RESUMO

OBJECTIVE: This paper aims to investigate the clinical efficacy of B-ultrasound-guided puncture in the treatment of incision infection after total abdominal hysterectomy (TAH) and to provide references for the clinical treatment. PATIENTS AND METHODS: 116 patients with uterine incision infection after TAH were selected and randomly divided into the observation group and the control group, with 58 cases in each group. The patients in the control group received an intravenous drip of ceftazidime and tinidazole to prevent infection, and the patients in the observation group received B-ultrasound-guided puncture treatment on the basis of the treatment plan of the control group. The clinical therapeutic effects between the two groups were compared. RESULTS: The cure rate of excellence in the observation group was 84.48%, and the cure rate in the control group was 53.45%, while the difference between the two groups was statistically significant (p<0.05). The total effective rate in the observation group was 98.28%, and that in the control group was 87.93%, but there was no statistically significant difference between the two groups. The hospital stay was (9.5±1.6) days in the observation group and (12.3±2.1) days in the control group, and the mean hospital stay in the observation group was significantly shorter than that in the control group; the difference between the two groups was statistically significant (p<0.05). CONCLUSIONS: TAH should be performed on patients when they are in the best physical condition, and strictly according to the operation steps to reduce the duration of surgery. The application of B-ultrasound-guided puncture can effectively improve the excellent recovery rate of the incision infection after TAH and shorten the hospitalization time. It is worth popularizing in clinical practice.


Assuntos
Ceftazidima/administração & dosagem , Histerectomia/efeitos adversos , Punções/métodos , Infecção da Ferida Cirúrgica/terapia , Tinidazol/administração & dosagem , Adulto , Idoso , Estudos de Casos e Controles , Ceftazidima/uso terapêutico , Feminino , Humanos , Infusões Intravenosas , Laparoscopia/efeitos adversos , Tempo de Internação , Pessoa de Meia-Idade , Distribuição Aleatória , Infecção da Ferida Cirúrgica/etiologia , Tinidazol/uso terapêutico , Resultado do Tratamento , Ultrassonografia de Intervenção
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