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1.
Parasitol Int ; 73: 101972, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31400466

RESUMO

We examined lingual tissues of Japanese wild boars (Sus scrofa leucomystax) captured in the Amakusa Islands off the coast of Kumamoto Prefecture. One hundred and forty wild boars were caught in 11 different locations in Kamishima (n = 36) and Shimoshima (n = 104) in the Amakusa Islands, Japan between January 2016 and April 2018. Lingual tissues were subjected to histological examinations, where helminths and their eggs were observed in the epithelium of 51 samples (36.4%). No significant differences in prevalence were observed according to maturity, sex or capture location. Lingual tissues positive for helminth infection were randomly selected and intact male and female worms were collected for morphological measurements. Based on the host species, site of infection, and morphological details, we identified the parasite as Eucoleus garfiai (Gállego et Mas-Coma, 1975) Moravec, 1982 (syn. Capillaria garfiai). This is the first report from outside Europe of E. garfiai infection in wild boars. Phylogenetic analysis of the parasite using the 18S ribosomal RNA gene sequence confirmed that the parasite grouped with other Eucoleus species, providing additional nucleotide sequence for this genus. Since wild boar populations are widely distributed in Japan, continuing surveys on the epidemiology of the parasite and identifying possible intermediate host candidates are crucial for elucidating the transmission route of the parasite.


Assuntos
Infecções por Enoplida/veterinária , Doenças dos Suínos/epidemiologia , Trichuroidea/fisiologia , Animais , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Feminino , Japão/epidemiologia , Masculino , Filogenia , Prevalência , RNA de Helmintos/análise , RNA Ribossômico 18S/análise , Sus scrofa , Suínos , Doenças dos Suínos/parasitologia , Língua/parasitologia , Trichuroidea/anatomia & histologia , Trichuroidea/classificação
2.
J Vis Exp ; (130)2017 12 08.
Artigo em Inglês | MEDLINE | ID: mdl-29286459

RESUMO

Health monitoring systems are developed and used in zebrafish research facilities because pathogens of Danio rerio such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa have the potential to impair animal welfare and research. The fish are typically analyzed post mortem to detect microbes. The use of sentinels is a suggested way to improve the sensitivity of the surveillance and to reduce the number of animals to sample. The setting of a pre-filtration sentinel tank out of a recirculating system is described. The technique is developed to prevent water pollution and to represent the fish population by a careful selection of age, gender, and strains. In order to use the minimum number of animals, techniques to screen the environment are also detailed. Polymerase Chain Reaction (PCR) on surface sump swabs is used to significantly improve the detection of some prevalent and pathogenic mycobacterial species such as Mycobacterium fortuitum, Mycobacterium haemophilum, and Mycobacterium chelonae. Another environmental method consists of processing the sludge at the bottom of a holding tank or sump to look for P. tomentosa eggs. This is a cheap and fast technique that can be applied in quarantine where a breeding device is submerged into the holding tank of imported animals. Finally, PCR is applied to the sludge sample and A. hydrophila is detected at the sump's bottom and surface. Generally, these environmental screening techniques applied to these specific pathogens have led to an increased sensitivity compared to the testing of pre-filtration sentinels.


Assuntos
Aeromonas hydrophila/isolamento & purificação , Mycobacterium/isolamento & purificação , Trichuroidea/isolamento & purificação , Peixe-Zebra/microbiologia , Animais , Doenças dos Peixes/parasitologia
3.
J Fish Dis ; 40(2): 169-182, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27334246

RESUMO

Pseudocapillaria tomentosa is a common pathogen of zebrafish (Danio rerio) in research facilities. We developed a method to collect and concentrate the nematode eggs using a modified sugar centrifugation method and documented their normal development. Embryonating stages with blastomere formation followed by elongation of the embryo prior to larva formation cumulated in developed larvae inside the eggs and hatching after 5-10 day. We then evaluated the efficacy of heat and chlorine to kill them based on a larva development assay. Eggs were exposed to 40, 50, 60 °C for 30 min and 1 h. Chlorine treatment was performed at 100, 250, 500, 1000, 3000 and 6000 ppm for 10 min. Samples exposed to 40 °C for 30 min or 1 h showed incidences of larvated eggs similar to controls. In contrast, no larvation occurred with eggs exposed to either 50 or 60 °C for 30 min or 1 h. Remarkably, in repeated assays, samples exposed to low doses of chlorine (100, 250, 500 and 1000 ppm for 10 min) showed significantly higher incidence of larvation than controls. Eggs treated with 3000 ppm for 10 min did not develop larvae, and no eggs were found after 6000 ppm treatment.


Assuntos
Antinematódeos/farmacologia , Aquicultura/métodos , Infecções por Enoplida/veterinária , Doenças dos Peixes/prevenção & controle , Trichuroidea/efeitos dos fármacos , Peixe-Zebra , Animais , Cloro/farmacologia , Relação Dose-Resposta a Droga , Infecções por Enoplida/parasitologia , Infecções por Enoplida/prevenção & controle , Doenças dos Peixes/parasitologia , Temperatura Alta , Óvulo/efeitos dos fármacos , Trichuroidea/embriologia
4.
Rev Bras Parasitol Vet ; 25(3): 374-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27580395

RESUMO

Parasitic diseases reflect the health and balance of ecosystems, affecting not only individuals but also entire populations or communities. The aim of this study was to report on the diversity of parasitic helminths detected in the feces of a wild feline in southern Brazil. Parasites were obtained from fecal samples, and four techniques were used for parasitological examination: direct examination, centrifugal flotation with zinc sulfate (Faust technique), simple sedimentation (Hoffman technique) and Baermann-Moraes. The parasites were identified through micrometry and morphology, as follows: Ancylostoma sp., Toxocara sp., Trichuridae, Aelurostrongylus abstrusus, Alaria sp., and Spirometra sp. We recorded the genus Ancylostoma parasitizing L. colocolo for the first time.


Assuntos
Fezes/parasitologia , Felidae/parasitologia , Helmintos/isolamento & purificação , Ancylostoma/isolamento & purificação , Animais , Brasil , Spirometra/isolamento & purificação , Estrongilídios/isolamento & purificação , Toxocara/isolamento & purificação , Trichuroidea/isolamento & purificação
5.
Rev. bras. parasitol. vet ; 25(3): 374-377, July-Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-795070

RESUMO

Abstract Parasitic diseases reflect the health and balance of ecosystems, affecting not only individuals but also entire populations or communities. The aim of this study was to report on the diversity of parasitic helminths detected in the feces of a wild feline in southern Brazil. Parasites were obtained from fecal samples, and four techniques were used for parasitological examination: direct examination, centrifugal flotation with zinc sulfate (Faust technique), simple sedimentation (Hoffman technique) and Baermann-Moraes. The parasites were identified through micrometry and morphology, as follows: Ancylostoma sp., Toxocara sp., Trichuridae, Aelurostrongylus abstrusus, Alaria sp., and Spirometra sp. We recorded the genus Ancylostoma parasitizing L. colocolo for the first time.


Resumo Doenças parasitárias refletem a saúde e o equilíbrio dos ecossistemas, influenciando não só um indivíduo e sim uma população ou comunidade. Este trabalho teve por objetivo relatar a diversidade de helmintos encontradas nas fezes de um felino silvestre na região Sul do Brasil. Os parasitos foram obtidos a partir de amostras fecais, sendo utilizadas quatro técnicas para os exames parasitológicos: exame direto, centrífugo-flutuação com sulfato de zinco (Técnica de Faust), sedimentação simples (Técnica de Hoffman) e Baermann-Moraes. Os parasitos foram identificados através de micrometria e morfologia, sendo esses: Ancylostoma sp., Toxocara sp., Trichuridae, Aelurostrongylus abstrusus, Alaria sp. e Spirometra sp. Estudos da fauna parasitária de animais silvestres são relevantes, tanto para o equilíbrio e saúde desses animais, como para o controle e prevenção de doenças transmitidas ao homem. Ancylostoma spp. foi identificado pela primeira vez em L. colocolo.


Assuntos
Animais , Felidae/parasitologia , Fezes/parasitologia , Helmintos/isolamento & purificação , Spirometra/isolamento & purificação , Toxocara/isolamento & purificação , Trichuroidea/isolamento & purificação , Brasil , Estrongilídios/isolamento & purificação , Ancylostoma/isolamento & purificação
6.
Vet Q ; 36(3): 145-9, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27161590

RESUMO

BACKGROUND: Capillariasis, an important parasitic disease of birds is caused at least by seven different genera of trichurid nematodes with clinical outcome ranging from mild enteritis to high mortality. OBJECTIVE: This study was aimed to investigate the causative agent involved in high mortality associated with severe enteric illness among turkey flocks in an organized commercial poultry farm at Bareilly, India. MATERIALS AND METHODS: Turkey carcasses (n = 119) and fecal samples from the affected deep litter pen constituted as the study materials. The disease was investigated by systematic necropsy, direct microscopy and histopathology. Representative samples were screened for other enteric pathogens. RESULTS: Microscopic examination of mucosal scraping revealed capillarid worms and their eggs in all the samples. The morphological features of adult worms were typically consistent to Baruscapillaria obsignata. Histopathology exhibited thickened muscular and mucosal layers, mononuclear and heterophilic infiltration in the lamina propria, blunting and clubbing of villi, epithelial denudation and sections of capillarid worms. Administration of levamisole at 80 ppm in drinking water reduced the mortality, clinical illness and worm load after three days of therapy. CONCLUSIONS: The capillarid worms in different avian hosts can cause different clinical manifestations and outcomes. From India, this is the first report describing intestinal pathology caused by B. obsignata in turkeys. We conclude that the B. obsignata infection is capable of causing life threatening enteropathy in turkeys and, hence, routine screening, scheduled deworming and good litter management are crucial to control the infection and its associated loss.


Assuntos
Infecções por Enoplida/veterinária , Enterite/veterinária , Doenças das Aves Domésticas/patologia , Trichuroidea/isolamento & purificação , Perus , Animais , Infecções por Enoplida/parasitologia , Infecções por Enoplida/patologia , Enterite/parasitologia , Enterite/patologia , Feminino , Índia , Masculino , Doenças das Aves Domésticas/parasitologia , Trichuroidea/anatomia & histologia
7.
Zebrafish ; 13 Suppl 1: S132-7, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27182750

RESUMO

Considering the numbers of zebrafish held in the laboratories, it is relevant to develop some tools to monitor the health of the animals, as well as their biotope. Environmental samples can be used to detect aquatic pathogens. Comprehensive health monitoring would thus seek pathogens in three dimensions of the animals and microbes' habitat: the fish, the sludge, and the water. This three-dimensional approach is called the 3D screen and it introduces some complementary tools to routine sentinel screening. For example, sludge and sump swabs analyses allow an efficient detection of pathogens at a low cost and with a fast turnover. These assays are particularly useful in cases of Pseudocapillaria tomentosa infestation or Mycobacterium haemophilum outbreak. Indeed, such a broader choice of diagnostic tests gives flexibility for the veterinarian to investigate Mycobacterium spp. presence in the water systems and fish colonies. Some other robust additional analysis, like the mortality rate monitoring, quickens the decision-making process. The 3D screen describes how this new toolbox can be used efficiently to monitor laboratory fish health.


Assuntos
Criação de Animais Domésticos/métodos , Aquicultura/métodos , Infecções por Enoplida/veterinária , Doenças dos Peixes/prevenção & controle , Infecções por Mycobacterium/veterinária , Vigilância de Evento Sentinela/veterinária , Peixe-Zebra , Bem-Estar do Animal , Animais , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Infecções por Enoplida/prevenção & controle , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/microbiologia , Doenças dos Peixes/parasitologia , Infecções por Mycobacterium/epidemiologia , Infecções por Mycobacterium/microbiologia , Infecções por Mycobacterium/prevenção & controle , Mycobacterium haemophilum/isolamento & purificação , Trichuroidea/isolamento & purificação
8.
Rev Bras Parasitol Vet ; 23(3): 399-402, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25271463

RESUMO

Pterothominx pulchra (Freitas, 1934) are little known gastric nematodes of Nyctinomops laticaudatus (Chiroptera: Molossidae). Information about the occurrence and host range of these parasites in Neotropical region is still scanty, and the only two morphological descriptions available in the literature are divergent about the presence or absence of a spiny spicular sheath in males, which may lead to incorrect taxonomical positioning, since this feature represents the main difference between the genera Pterothominx and Aonchotheca. Based on the absence of this morphological feature in specimens of this nematode obtained from N. laticaudatus and Nyctinomops macrotis bats captured in two municipalities in the state of São Paulo, Brazil, the present study reclassifies the aforementioned species in the genus Aonchotheca and allocates it to the subgenus Aonchotheca. Additional morphometric data and new host and locality records are also provided.


Assuntos
Quirópteros/parasitologia , Trichuroidea/anatomia & histologia , Trichuroidea/classificação , Animais , Feminino , Masculino , Trichuroidea/fisiologia
9.
Zebrafish ; 11(5): 490-7, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25237985

RESUMO

Tolerance of adult zebrafish and efficacy of emamectin benzoate and ivermectin in eliminating Pseudocapillaria tomentosa infection were evaluated. In the tolerance study, behavioral changes, fecundity, histopathology, and mortality were evaluated for in-feed administration of emamectin (0.05, 0.10, and 0.25 mg/kg) and ivermectin (0.05 and 0.10 mg/kg). All doses of emamectin were well tolerated. Ivermectin 0.05 mg/kg administration resulted in mild behavioral changes and a transient decrease in fecundity. Ivermectin 0.10 mg/kg administration resulted in severe behavioral changes and some mortality. In the efficacy study, emamectin (0.05 and 0.25 mg/kg) and ivermectin (0.05 mg/kg) were evaluated for their efficacy in eliminating P. tomentosa infection. Emamectin reduced parasite burden in infected zebrafish, and ivermectin eliminated intestinal nematode infections. Despite a small margin of safety, ivermectin 0.05 mg/kg was effective at eliminating P. tomentosa infection in adult zebrafish. Higher doses or a longer course of treatment may be needed for complete elimination of P. tomentosa infection using emamectin. In this study, we propose two possible treatments for intestinal nematode infections in zebrafish.


Assuntos
Antinematódeos/farmacologia , Infecções por Enoplida/veterinária , Doenças dos Peixes/tratamento farmacológico , Ivermectina/análogos & derivados , Ivermectina/farmacologia , Trichuroidea/efeitos dos fármacos , Peixe-Zebra , Animais , Antinematódeos/efeitos adversos , Antinematódeos/uso terapêutico , Infecções por Enoplida/tratamento farmacológico , Infecções por Enoplida/parasitologia , Feminino , Doenças dos Peixes/parasitologia , Ivermectina/efeitos adversos , Ivermectina/uso terapêutico , Masculino
10.
Rev. bras. parasitol. vet ; 23(3): 399-402, Jul-Sep/2014. graf
Artigo em Inglês | LILACS | ID: lil-722725

RESUMO

Pterothominx pulchra (Freitas, 1934) are little known gastric nematodes of Nyctinomops laticaudatus (Chiroptera: Molossidae). Information about the occurrence and host range of these parasites in Neotropical region is still scanty, and the only two morphological descriptions available in the literature are divergent about the presence or absence of a spiny spicular sheath in males, which may lead to incorrect taxonomical positioning, since this feature represents the main difference between the genera Pterothominx and Aonchotheca. Based on the absence of this morphological feature in specimens of this nematode obtained from N. laticaudatus and Nyctinomops macrotis bats captured in two municipalities in the state of São Paulo, Brazil, the present study reclassifies the aforementioned species in the genus Aonchotheca and allocates it to the subgenus Aonchotheca. Additional morphometric data and new host and locality records are also provided.


Pterothominx pulchra (Freitas, 1934) são nematódeos gástricos pouco conhecidos de Nyctinomops laticaudatus (Chiroptera: Molossidae). Informações referentes à ocorrência e variedade de hospedeiros desses parasitos ainda são escassas na região Neotropical. As únicas duas descrições morfológicas disponíveis na literatura são divergentes entre si quanto à presença de bainha espicular espinhosa nos machos, o que resultou no posicionamento taxonômico equivocado desta espécie, uma vez que, é a principal característica para diagnóstico dos gêneros Pterothominx e Aonchotheca. Baseado na ausência dessa característica morfológica em espécimes desse nematódeo, obtidos em morcegos N. laticaudatus e Nyctinomops macrotis capturados em dois municípios do Estado de São Paulo, Brasil, o presente estudo classifica a referida espécie no gênero Aonchotheca e a aloca no subgênero Aonchotheca, além de fornecer dados morfométricos adicionais e novos registros de hospedeiro e localidades.


Assuntos
Animais , Feminino , Masculino , Quirópteros/parasitologia , Trichuroidea/anatomia & histologia , Trichuroidea/classificação , Trichuroidea/fisiologia
11.
Zebrafish ; 10(3): 447-50, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23808385

RESUMO

Abstract Our zebrafish colony experienced a period of increased mortality rate of 6.5 times more deaths per month in a colony of over 13,000 zebrafish (Danio rerio), which developed over 3 months. We observed that before death, affected fish appeared emaciated, often with an abdominal bulge. We performed dissection on 18 fish that had this appearance and found in 15 that their gut was infected with a nematode that closely resembled Pseudocapillaria tomentosa. We devised a treatment protocol for this nematode infection, which involved addition of fenbendazole, a drug used to treat nematode infections in cattle and sheep, to the fish feed. Fenbendazole produced no severe side effects in the fish and several treatments have effectively eradicated the parasite from our colony. The mortality rate of our fish has decreased to a value of 0.7%/month (p<0.001, equal to that before the infection). We propose this protocol as an inexpensive alternative to having to rederive an entire colony from bleached eggs, and as a prophylactic measure used in quarantine facilities on a regular basis.


Assuntos
Antinematódeos/uso terapêutico , Infecções por Enoplida/veterinária , Fenbendazol/uso terapêutico , Enteropatias Parasitárias/veterinária , Peixe-Zebra/parasitologia , Criação de Animais Domésticos , Animais , Infecções por Enoplida/tratamento farmacológico , Feminino , Enteropatias Parasitárias/tratamento farmacológico , Masculino , Trichuroidea/isolamento & purificação
12.
J Parasitol ; 99(4): 624-33, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23413958

RESUMO

Twenty-eight Capillariinae species have been recorded in rodents; 1 of these species was reported from a caviomorph rodent, Hydrochoeris hydrochaeris (capybara), and placed in the genus Echinocoleus by Moravec (1982). However, both original description and subsequent contributions of Echinocoleus hydrochoeri are poor and incomplete. In this paper, this species is redescribed, and a new geographical distribution is reported. The redescription is based on morphologic and morphometrical features; intestine ends in a cloaca beside ejaculatory duct, caudal bursa composed of 2 large ventrolateral lobes with a fleshy internal part and a membranous external part (they are not united dorsally with a membrane), 1 pair of caudal papillae, terminal part of cylindrical cirrus ornamented with thin and thick spines (and particular pattern distribution), sclerotized spicule in male, and vulvar appendage in female, and 3 bacillary bands (1 ventral and 2 lateral). Generic and specific analyses were performed to establish new standards for future studies on the systematic position of Capillariinae species. This study presents new morphological information and a new record of a capillariid species from Argentina.


Assuntos
Infecções por Enoplida/veterinária , Doenças dos Roedores/parasitologia , Trichuroidea/classificação , Animais , Argentina , Infecções por Enoplida/parasitologia , Feminino , Intestino Delgado/parasitologia , Masculino , Microscopia Eletrônica de Varredura/veterinária , Roedores , Estômago/parasitologia , Trichuroidea/anatomia & histologia , Trichuroidea/ultraestrutura
13.
Zootaxa ; 3599: 577-87, 2013 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-24614031

RESUMO

Nematodes, comprising 2 species, a new genus from the family Syphaciidae and a new species from the family Trichuridae were collected from the lower digestive tracts of 4 species of Pogonomys; P. championi, Flannery (12 individuals), P. loriae, Thomas (14 individuals), P. macrourus, (Milne Edwards) (19 individuals) and P. sylvestris, Thomas (27 individuals) from Papua, Indonesia and Papua New Guinea. Pogonomicola rugala n. gen., n. sp. differs from all other genera in the Sypaciidae in having cervical alae with numerous folds and a single weakly defined mamelon. Trichuris germani n. sp. differs from all congeners, including the cosmopolitan T. muris, the only other trichurid reported from the region, by the lengths of the spicules and vagina, the ratio of anterior to posterior body length and the number of convolutions of the testis. The genus Pogonomys, with four species from four nematode families had a relatively rich helminth fauna in the lower digestive tract compared to other ansomyins studied. The Oxyuridae, with three genera comprising 5 species was the dominant group found in the lower digestive tract of the Anisomyini, indicating the possibility that the isolation of the old endemic rodents in New Guinea has been associated with a period of coevolution between anisomyin hosts and their syphaciine parasites.


Assuntos
Murinae/parasitologia , Oxyuroidea/classificação , Oxyuroidea/fisiologia , Trichuroidea/classificação , Trichuroidea/fisiologia , Animais , Ceco/parasitologia , Colo/parasitologia , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Infecções por Enoplida/veterinária , Feminino , Indonésia , Masculino , Oxiuríase/epidemiologia , Oxiuríase/parasitologia , Oxiuríase/veterinária , Oxyuroidea/anatomia & histologia , Papua Nova Guiné , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/parasitologia , Trichuroidea/anatomia & histologia
14.
Parasite ; 19(4): 433-5, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23193529

RESUMO

Dogs can be infected by several nematodes of the Trichuridae family. Trichuridae eggs are all similar, barrel shaped with polar plugs, and misdiagnosis among different species can occur. The most common species is Trichuris vulpis, while the respiratory parasites Eucoleus boehmi (syn. Capillaria boehmi) and Eucoleus aerophilus (syn. Capillaria aerophila) are rarely observed in pets. E. boehmi is reported for the first time in this study in north-western Italy with other Trichuridae. Dog faecal samples (270) were examined by flotation. E. boehmi (2.2%), E. aerophilus (4.4%) and T. vulpis (12.2%) were found; identification was done with measurements and through observation of morphological characters already known. The specific identification of E. boehmi was confirmed using scanning electron microscopy: its egg shell shows a dense network with a fine mesh, surrounding small pits, on the contrary E. aerophilus eggs present a thick mesh with wide depressions, while T. vulpis eggs surface is smooth.


Assuntos
Doenças do Cão/parasitologia , Infecções por Enoplida/veterinária , Trichuroidea/isolamento & purificação , Animais , Doenças do Cão/epidemiologia , Cães , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Fezes/parasitologia , Itália/epidemiologia , Microscopia Eletrônica de Varredura/veterinária , Óvulo/ultraestrutura , Trichuroidea/ultraestrutura
15.
Blood ; 116(19): 3944-54, 2010 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-20713961

RESUMO

Eosinophils are granulocytic leukocytes implicated in numerous aspects of immunity and disease. The precise functions of eosinophils, however, remain enigmatic. Alternative models to study eosinophil biology may thus yield novel insights into their function. Eosinophilic cells have been observed in zebrafish but have not been thoroughly characterized. We used a gata2:eGFP transgenic animal to enable prospective isolation and characterization of zebrafish eosinophils, and demonstrate that all gata2(hi) cells in adult hematopoietic tissues are eosinophils. Although eosinophils are rare in most organs, they are readily isolated from whole kidney marrow and abundant within the peritoneal cavity. Molecular analyses demonstrate that zebrafish eosinophils express genes important for the activities of mammalian eosinophils. In addition, gata2(hi) cells degranulate in response to helminth extract. Chronic exposure to helminth- related allergens resulted in profound eosinophilia, demonstrating that eosinophil responses to allergens have been conserved over evolution. Importantly, infection of adult zebrafish with Pseudocapillaria tomentosa, a natural nematode pathogen of teleosts, caused marked increases in eosinophil number within the intestine. Together, these observations support a conserved role for eosinophils in the response to helminth antigens or infection and provide a new model to better understand how parasitic worms activate, co-opt, or evade the vertebrate immune response.


Assuntos
Eosinófilos/fisiologia , Peixe-Zebra/sangue , Animais , Animais Geneticamente Modificados , Antígenos de Helmintos , Sequência de Bases , Degranulação Celular/imunologia , Primers do DNA/genética , Infecções por Enoplida/sangue , Infecções por Enoplida/imunologia , Infecções por Enoplida/parasitologia , Eosinofilia/etiologia , Eosinofilia/imunologia , Eosinofilia/parasitologia , Eosinófilos/citologia , Eosinófilos/imunologia , Eosinófilos/parasitologia , Fator de Transcrição GATA2/genética , Fator de Transcrição GATA2/metabolismo , Interações Hospedeiro-Parasita , Neutrófilos/fisiologia , Trichuroidea/imunologia , Trichuroidea/patogenicidade , Peixe-Zebra/genética , Peixe-Zebra/imunologia , Peixe-Zebra/parasitologia , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo
16.
Pol J Vet Sci ; 11(2): 143-5, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18683544

RESUMO

The present study investigated the efficacy of topical selamectin for elimination of naturally acquired Trichosomoides crassicauda infection in rats. Twelve T. crassicauda-positive rats were assigned to the treatment group and six rats were assigned to the control group. Selamectin (6 mg/kg) was applied topically to the skin in a single spot at the base of the neck in front of the scapulae in the treatment group. To assess the efficacy of the treatment, animal faeces were investigated with the use of the flotation technique on days 0, 4, 14 and 24 after selamectin application. The rats of the treatment and control groups were necropsied on the day 24. In the treatment group, 7 of 12 infected rats were cured completely. Topical selamectin was found to be effective in eliminating T. crassicauda infection in rats.


Assuntos
Antiparasitários/uso terapêutico , Infecções por Enoplida/veterinária , Ivermectina/análogos & derivados , Doenças dos Roedores/tratamento farmacológico , Administração Tópica , Animais , Infecções por Enoplida/tratamento farmacológico , Fezes/parasitologia , Ivermectina/uso terapêutico , Distribuição Aleatória , Ratos , Fatores de Tempo , Resultado do Tratamento , Trichuroidea
17.
J Parasitol ; 93(2): 377-82, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17539422

RESUMO

Huffmanela moraveci n. sp. (Nematode: Trichosomoididae) is described from the skin of fins and gills of the silversides Odontesthes smitti Lahille, 1949, and Odontesthes nigricans Richardson, 1848 (Pisces: Atheriniformes), from southwestern Atlantic coast, on the basis of the morphology of adults and eggs found in the host tissues. Huffmanela moraveci n. sp. can be distinguished from Huffmanela carcharini, H. banningi, H. japonica, H. mexicana, H. paronai, H. schouteni, H. shikokuensis, H. branchialis, H. filamentosa, H. ossicola, and H. lata by the size and shape of the eggs. The adult stage of H. moraveci n. sp. can be differentiated from H. hufmani and H. canadensis by the position of the nerve ring and by the total esophagus length/body length ratio. Huffmanela moraveci n. sp. also differs from H. huffmani by the presence of bacillary bands and by the body length. This is the first report of a species of Huffmanela in South America, the first report of a species of Huffmanela with Odontesthes spp. as host, and the third known adult form in the genus.


Assuntos
Infecções por Enoplida/veterinária , Doenças dos Peixes/parasitologia , Smegmamorpha/parasitologia , Trichuroidea/classificação , Animais , Argentina , Ectoparasitoses/parasitologia , Ectoparasitoses/veterinária , Infecções por Enoplida/parasitologia , Feminino , Brânquias/parasitologia , Masculino , Microscopia Eletrônica de Varredura/veterinária , Trichuroidea/anatomia & histologia , Trichuroidea/ultraestrutura
18.
Parassitologia ; 47(2): 241-5, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16252480

RESUMO

In order to assess the parasitological status in imported pheasants, 51 birds (Phasianus colchicus) coming from Poland and Rumania and used in Italy in repopulation interventions for hunting purposes were examined. From each animal the trachea, oesophagus, crop and intestine were collected and examined for the presence of nematodes. The examination of the oesophagi and crops of 5 birds revealed the presence of parasites pertaining to the family Capillariidae: Eucoleus contortus was found in all of the 5 animals, E. annulatus was present along with the previous parasite in one animal coming from Poland. The examination of the tracheae revealed the presence of Syngamus trachea in 5 animals (9.80%). Adult or larval stages of Heterakis gallinarum (37.25% of birds) and Capillariidae (35.29%) were found in the intestinal tracts. Aonchotheca caudinflata was detected only in one bird coming from Poland; the capillarids found in all of the remaining pheasants exhibited morphological characteristics referable to Capillaria phasianina, a species never reported in Italy. The release of game from foreign countries, therefore, may always constitute a risk for the autochthonous one due to the spread of new parasitic infections.


Assuntos
Doenças das Aves/parasitologia , Galliformes/parasitologia , Infecções por Nematoides/veterinária , Animais , Ascaridídios/crescimento & desenvolvimento , Ascaridídios/isolamento & purificação , Infecções por Ascaridida/epidemiologia , Infecções por Ascaridida/parasitologia , Infecções por Ascaridida/veterinária , Doenças das Aves/epidemiologia , Papo das Aves/parasitologia , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Infecções por Enoplida/veterinária , Esôfago/parasitologia , Feminino , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Enteropatias Parasitárias/veterinária , Intestinos/parasitologia , Larva , Masculino , Metastrongyloidea/isolamento & purificação , Infecções por Nematoides/epidemiologia , Infecções por Nematoides/parasitologia , Polônia/epidemiologia , Romênia/epidemiologia , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologia , Infecções por Strongylida/veterinária , Traqueia/parasitologia , Trichuroidea/isolamento & purificação
19.
Mem. Inst. Oswaldo Cruz ; 99(7): 721-726, Nov. 2004. ilus, tab
Artigo em Inglês | LILACS | ID: lil-391601

RESUMO

The present investigation is related to the frequency of infection and to the gross and microscopic lesions associated to the presence of trichurid worms in 50 ring-necked pheasants (Phasianus colchicus) from backyard flocks in the state of Rio de Janeiro, Brazil. In the investigated birds, the overall infection rate was of 74 percent, with the presence of Eucoleus perforans with 72 percent of prevalence and 21.2 of mean intensity, in the esophageal and crop mucosa and rarely in the junction of the proventriculus and esophagus, E. annulatus with 2 percent and 3 in the crop mucosa, Capillaria phasianina, with 12 percent and 4.3 in the cecum and small intestine and Baruscapillaria obsignata, for the first time referred in this host, with 2 percent and 1 in the small intestine. Clinical signs were absent. The gross lesions observed in the crop and esophagus of 14 (38.9 percent) pheasants parasitized with E. perforans were thickening, small nodules, congestion, and petechial haemorrhages in the mucosa. These birds presented a mean infection of 37.5 and a range of infection of 10-82. The microscopic lesions revealed chronic esophagitis with diffuse inflammatory process in the lamina propria characterized mostly by a mononuclear cell infiltrate and also with the presence of granulocytes. In the case of the parasitism of pheasants with C. phasianina, the gross lesions were absent; microscopic lesions were characterized by chronic typhlitis with mononuclear infiltrate. Gross and microscopic lesions were absent in the pheasants parasitized with E. annulatus and B. obsignata.


Assuntos
Animais , Masculino , Feminino , Doenças das Aves , Infecções por Enoplida , Trichuroidea , Brasil , Infecções por Enoplida , Prevalência
20.
Mem Inst Oswaldo Cruz ; 99(7): 721-6, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15654428

RESUMO

The present investigation is related to the frequency of infection and to the gross and microscopic lesions associated to the presence of trichurid worms in 50 ring-necked pheasants (Phasianus colchicus) from backyard flocks in the state of Rio de Janeiro, Brazil. In the investigated birds, the overall infection rate was of 74%, with the presence of Eucoleus perforans with 72% of prevalence and 21.2 of mean intensity, in the esophageal and crop mucosa and rarely in the junction of the proventriculus and esophagus, E. annulatus with 2% and 3 in the crop mucosa, Capillaria phasianina, with 12% and 4.3 in the cecum and small intestine and Baruscapillaria obsignata, for the first time referred in this host, with 2% and 1 in the small intestine. Clinical signs were absent. The gross lesions observed in the crop and esophagus of 14 (38.9%) pheasants parasitized with E. perforans were thickening, small nodules, congestion, and petechial haemorrhages in the mucosa. These birds presented a mean infection of 37.5 and a range of infection of 10-82. The microscopic lesions revealed chronic esophagitis with diffuse inflammatory process in the lamina propria characterized mostly by a mononuclear cell infiltrate and also with the presence of granulocytes. In the case of the parasitism of pheasants with C. phasianina, the gross lesions were absent; microscopic lesions were characterized by chronic typhlitis with mononuclear infiltrate. Gross and microscopic lesions were absent in the pheasants parasitized with E. annulatus and B. obsignata.


Assuntos
Doenças das Aves/epidemiologia , Infecções por Enoplida/veterinária , Galliformes/parasitologia , Trichuroidea/isolamento & purificação , Animais , Doenças das Aves/patologia , Brasil/epidemiologia , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/patologia , Feminino , Galliformes/classificação , Masculino , Prevalência , Trichuroidea/classificação
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