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1.
Toxins (Basel) ; 14(7)2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35878187

RESUMO

Type I ribosome-inactivating proteins (RIPs) are plant toxins that inhibit protein synthesis by exerting rRNA N-glycosylase activity (EC 3.2.2.22). Due to the lack of a cell-binding domain, type I RIPs are not target cell-specific. However once linked to antibodies, so called immunotoxins, they are promising candidates for targeted anti-cancer therapy. In this study, sapovaccarin-S1 and -S2, two newly identified type I RIP isoforms differing in only one amino acid, were isolated from the seeds of Saponaria vaccaria L. Sapovaccarin-S1 and -S2 were purified using ammonium sulfate precipitation and subsequent cation exchange chromatography. The determined molecular masses of 28,763 Da and 28,793 Da are in the mass range typical for type I RIPs and the identified amino acid sequences are homologous to known type I RIPs such as dianthin 30 and saporin-S6 (79% sequence identity each). Sapovaccarin-S1 and -S2 showed adenine-releasing activity and induced cell death in Huh-7 cells. In comparison to other type I RIPs, sapovaccarin-S1 and -S2 exhibited a higher thermostability as shown by nano-differential scanning calorimetry. These results suggest that sapovaccarin-S1 and -S2 would be optimal candidates for targeted anti-cancer therapy.


Assuntos
Saponaria , Vaccaria , N-Glicosil Hidrolases/química , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/farmacologia , Isoformas de Proteínas , Proteínas Inativadoras de Ribossomos/metabolismo , Proteínas Inativadoras de Ribossomos Tipo 1/química , Ribossomos/metabolismo , Saponaria/química , Saponaria/metabolismo , Sementes/química
2.
Zhongguo Zhong Yao Za Zhi ; 46(13): 3388-3393, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34396759

RESUMO

To study the mechanism of polysaccharides from seeds of Vaccaria segetalis( PSV) in the treatment of bacterial cystitis through the NLRP3 inflammasome pathway. The rat model of urinary tract infection was used and treated with PSV,and the urine and bladders were collected. The level of interleukin-10( IL-10) in rat urine was detected by enzyme linked immunosorbent assay( ELISA). Western blot and immunofluorescence staining were used to detect the expressions of sonic hedgehog( SHH) and NLRP3 inflammasome [NOD-like receptor thermoprotein domain 3( NLRP3),apoptosis associated speck like protein( ASC) and pro-caspase-1]. The expression of Toll-like receptor pathway was detected by RT-PCR. The death of 5637 cells induced by uropathogenic Escherichia coli( UPEC) and lactate dehydrogenase( LDH) release were evaluated using live/dead staining. The results showed that in the rat bladder,the expressions of SHH,NLRP3 inflammasomes and Toll-like receptors were significantly up-regulated,and NLRP3 inflammasomes were significantly activated by UPEC infection. The administration with PSV could significantly increase the concentration of IL-10 in urine,inhibit the expressions of SHH,NLRP3 inflammasomes and Toll-like receptors in bladder,and inhibit the activation of NLRP3 inflammasomes. A large number of 5637 cells were dead after UPEC infection and caused LDH production. PSV could significantly inhibit the death of 5637 cells and the release of LDH. In conclusion,PSV could inhibit the expression and activation of NLRP3 inflammasomes by inhibiting the Toll-like receptor pathway,thereby mitigating the bladder injury.


Assuntos
Infecções Urinárias , Vaccaria , Animais , Proteínas Hedgehog , Inflamassomos/genética , Interleucina-1beta , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Polissacarídeos/farmacologia , Ratos , Sementes , Bexiga Urinária , Infecções Urinárias/tratamento farmacológico
3.
Methods Mol Biol ; 2289: 263-270, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34270076

RESUMO

The production of doubled haploid (DH) plants from microspores is an important technique used in plant breeding and basic research. DH technology is a rapid method for developing homozygous lines, which can be used to accelerate crop improvement programs. Haploidy technology can also be used in mutagenesis, transformation, and basic research such as genomic, biochemical, and physiological studies. There is no general protocol that will result in the production of DH in all species, as differences occur among species and among genotypes within a species in terms of embryogenic response. Here we describe methodology for developing doubled haploids in cow cockle (Saponaria vaccaria L.).


Assuntos
Melhoramento Vegetal/métodos , Saponaria/genética , Vaccaria/genética , Flores/genética , Genótipo , Haploidia , Mutagênese/genética
4.
Pharm Biol ; 59(1): 547-556, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33962551

RESUMO

CONTEXT: Achyranthes bidentata Blume (Amaranthaceae) (ABR) and semen vaccariae (SV) are used commonly in the clinical treatment of erectile dysfunction in males with diabetes mellitus (DMED) to strengthen the kidney and promote blood circulation, and often achieve good curative effects. OBJECTIVE: Explore mechanistic details of ABR + SV treatment against DMED. MATERIALS AND METHODS: Prediction of key targets by network pharmacology. A rat model of DM was established by streptozotocin injection (55 mg/kg). Apomorphine (100 µg/kg) was injected into rats to screen the DMED model. Group C (n = 6) and group M (n = 6) were gavaged with deionized water; group T (n = 6) was given Achyranthis bidentatae radix-semen vaccariae granule suspension (2.5 g/kg). It lasted 8 weeks. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB) were used to measure the expression of tissue-related proteins and mRNA. RESULTS: The predicted key targets are albumin (ALB), caspase-3 (CASP3), vascular endothelial growth factor A (VEGFA), angiotensin-converting enzyme (ACE), and endothelial nitric oxide synthase (eNOS). Compared with the M group (0.52 ± 0.04; 0.50 ± 0.03; 0.49 ± 0.02; 0.23 ± 0.03), CASP3, VEGFA, and ACE protein expression reduced in the T group (0.39 ± 0.06; 0.34 ± 0.03; 0.39 ± 0.03), and eNOS protein expression increased (0.34 ± 0.03). CONCLUSION: ABR + SV can improve erectile function in DMED rats. This study provides a potential mechanism for the treatment of DMED with ABR + SV and can benefit from more patients.


Assuntos
Achyranthes , Diabetes Mellitus Experimental/tratamento farmacológico , Disfunção Erétil/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Vaccaria , Animais , Diabetes Mellitus Experimental/patologia , Modelos Animais de Doenças , Disfunção Erétil/patologia , Masculino , Extratos Vegetais/isolamento & purificação , Ratos , Ratos Sprague-Dawley , Resultado do Tratamento
5.
Arch Pharm (Weinheim) ; 354(4): e2000446, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33522644

RESUMO

Cyclopolypeptides are among the most predominant biomolecules in nature, especially those derived from plant seeds. This category of compounds has gained extraordinary attention due to remarkable variety of structures and valuable biofunctions. These congeners display enormous variation in terms of both structure and function and are the most significant biomolecules due to their widespread bioproperties. The estrogenic activity, immunosuppressive activity, cytotoxicity, vasorelaxant activity, and other properties possessed by cyclic peptides from seeds of plants make these congeners attractive leads for the drug discovery process. The current study covers the important structural features, structure-activity relationship, synthesis methods, and bioproperties of plant seeds-originated bioactive peptides from Vaccaria segetalis, Linum usitatissimum, and Goniothalamus leiocarpus, which may prove vital for the development of novel therapeutics based on a peptide skeleton.


Assuntos
Produtos Biológicos/química , Peptídeos/química , Compostos Fitoquímicos/química , Plantas Medicinais/química , Sementes/química , Produtos Biológicos/metabolismo , Produtos Biológicos/farmacologia , Linho/química , Goniothalamus/química , Humanos , Conformação Molecular , Peptídeos/metabolismo , Peptídeos/farmacologia , Compostos Fitoquímicos/metabolismo , Compostos Fitoquímicos/farmacologia , Vaccaria/química
6.
J Ethnopharmacol ; 267: 113505, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33141055

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: According to the Chinese Pharmacopoeia, the seeds of Vaccaria segetalis, a traditional medicinal herb, can be used for treating urinary diseases. The polysaccharides extract from V. segetalis seeds (VSP) has been shown to prevent urinary tract infections (UTIs). AIM OF THE STUDY: Investigate the effects of VSP on treating kidney infection induced by uropathogenic Escherichia coli (UPEC) and the underlying mechanisms. MATERIALS AND METHODS: Both in vivo and in vitro infection models were established with the UPEC strain CFT073. After oral administration of VSP, the levels of bacterial load, cathelicidin (CRAMP), Toll-like receptors (TLRs) in the kidney were evaluated. The expression of cathelicidin (LL-37) in human renal cell carcinoma cell line (A498) was tested after the treatment of VSP. RESULTS: In the kidneys of infection models, high-titer bacteria was detected. In the kidney of rat model, the expression of CRAMP was down-regulated, no significant change was observed in the levels of TLRs. After oral administration of VSP, the bacterial load was significantly decreased in rat and mouse models, and the levels of CRAMP and TLRs were significantly up-regulated in rat model. In vitro, the expression of LL-37 was significantly inhibited by CFT073. VSP up-regulated the expression of LL-37 in A498 cells. CONCLUSIONS: The up-regulation of cathelicidin expression may contribute to the therapeutic effects of VSP on kidney infection.


Assuntos
Peptídeos Catiônicos Antimicrobianos/metabolismo , Infecções por Escherichia coli/tratamento farmacológico , Rim/efeitos dos fármacos , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Sementes , Infecções Urinárias/tratamento farmacológico , Escherichia coli Uropatogênica/efeitos dos fármacos , Vaccaria , Proteínas Adaptadoras de Transporte Vesicular/genética , Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Animais , Peptídeos Catiônicos Antimicrobianos/genética , Carga Bacteriana , Linhagem Celular Tumoral , Modelos Animais de Doenças , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Feminino , Humanos , Rim/metabolismo , Rim/microbiologia , Camundongos Endogâmicos C3H , Fator 88 de Diferenciação Mieloide/genética , Fator 88 de Diferenciação Mieloide/metabolismo , Extratos Vegetais/isolamento & purificação , Polissacarídeos/isolamento & purificação , Ratos Sprague-Dawley , Sementes/química , Transdução de Sinais , Receptores Toll-Like/genética , Receptores Toll-Like/metabolismo , Infecções Urinárias/metabolismo , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/patogenicidade , Vaccaria/química
7.
Trials ; 21(1): 224, 2020 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-32093785

RESUMO

BACKGROUND: Acute abdomen is a common disease in the emergency department (ED) and usually results in huge medical expenditure. To relieve abdominal pain effectively and reduce bed occupancy rate in emergency rooms, electroacupuncture is a practical method in the treatment of abdominal pain. METHODS/DESIGN: Five hundred patients will be randomly and evenly divided into experimental and control groups. Both groups should have their basic information taken and their bilateral acupuncture points ( Hegu (LI 4), Neiguan (PC6), Zusanli (ST 36), Shangjuxu (ST37), Xiajuxu (ST39), Taichong (LR3), and Taibai (SP3)) will be intervened by electroacupuncture or vaccaria Seeds, in this clinical study. Electroacupuncture has been introduced to this experiment as an auxiliary technique. The experimental group will receive real electroacupuncture, but the control group will receive a placebo electroacupuncture in which transcutaneous electrical nerve stimulation will not be turned on. After the intervention, we will evaluate the difference in abdominal pain, the length of stay at the emergent observation ward, and the proportion of revisits with abdominal pain. DISCUSSION: In Taiwan, medical expenditure is increasing annually because of the higher bed occupancy caused by acute abdominal pain in the hospital. We expect that the combined treatment of electroacupuncture and modern medical treatment will not only reduce bed occupancy and the length of ED stay but also effectively decrease the rate of readmission and revisits by 72 h. By means of electroacupuncture, the spiraling cost of health care can eventually be reduced. TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT03199495. Registered on 27 June 2017.


Assuntos
Abdome Agudo/terapia , Eletroacupuntura , Serviços Médicos de Emergência , Ensaios Clínicos Controlados Aleatórios como Assunto , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Avaliação de Resultados em Cuidados de Saúde , Estudos Prospectivos , Vaccaria
8.
J Ethnopharmacol ; 260: 112578, 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31962152

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The seeds of Vaccaria segetalis (Neck.) Garcke is used for the treatment of urinary diseases in Traditional Chinese Medicine according to the Chinese Pharmacopoeia. Crude polysaccharides and the aqueous extract from the seeds of V. segetalis (SVCP) were proved to be effective on treating benign prostatic hyperplasia. AIM OF THE STUDY: The aim of this study was to test the effects of SVCP on urinary tract infection (UTI) induced by uropathogenic Escherichia coli (UPEC) strain CFT073 in the rat model and to investigate the underlying mechanisms. MATERIALS AND METHODS: A rat UTI model was established with the infection of UPEC strain CFT073. After oral administration of SVCP, the urinalysis and histological examination were evaluated. The levels of pro-inflammatory cytokines, procalcitonin (PCT) and polymeric Ig receptor (PIGR) were used to test the effects of SVCP on host immunity. The mRNA level of PapG in CFT073 was used to test the influence of SVCP on virulence factor. The effects of SVCP on the inhibition of bacterial adhesion were evaluated with mice UTI model. RESULTS: In the rat UTI model, the levels of bacterial load, white blood cells (WBC) and red blood cells (RBC) in urine and the pathological injury in the bladder were significantly up-regulated, the expression of PIGR in kidney was down-regulated, no significant change was observed on the pro-inflammatory cytokines in urine. After oral administration of SVCP for 3 days, the levels of bacterial load, WBC and RBC in urine were significantly decreased, the pathological injury in the bladder were remarkably inhibited. The expression of IL-6, IL-8 in urine and PIGR in kidney were significantly up-regulated by SVCP (200 mg/kg). SVCP showed no effect on the concentration of PCT in serum. SVCP failed to down-regulate the mRNA level of PapG in CFT073. In the mice UTI model, pre-treatment of SVCP failed to inhibit the intracellular bacterial load in the bladder. CONCLUSIONS: The therapeutic effects of SVCP on treating UTIs might result from the up-regulation of innate immunity in the kidney. SVCP can be used as an alternative therapeutic agent for UTIs.


Assuntos
Antibacterianos/farmacologia , Infecções por Escherichia coli/prevenção & controle , Imunidade Inata/efeitos dos fármacos , Fatores Imunológicos/farmacologia , Rim/efeitos dos fármacos , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Sementes , Infecções Urinárias/prevenção & controle , Escherichia coli Uropatogênica/efeitos dos fármacos , Vaccaria , Animais , Antibacterianos/isolamento & purificação , Aderência Bacteriana/efeitos dos fármacos , Carga Bacteriana , Citocinas/metabolismo , Modelos Animais de Doenças , Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Feminino , Interações Hospedeiro-Patógeno , Fatores Imunológicos/isolamento & purificação , Mediadores da Inflamação/metabolismo , Rim/imunologia , Rim/metabolismo , Rim/microbiologia , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Extratos Vegetais/isolamento & purificação , Polissacarídeos/isolamento & purificação , Ratos Sprague-Dawley , Sementes/química , Transdução de Sinais , Bexiga Urinária/efeitos dos fármacos , Bexiga Urinária/microbiologia , Infecções Urinárias/imunologia , Infecções Urinárias/metabolismo , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/imunologia , Escherichia coli Uropatogênica/patogenicidade , Vaccaria/química , Virulência/efeitos dos fármacos
9.
Acta Parasitol ; 64(3): 520-527, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31087260

RESUMO

INTRODUCTION: Trichinellosis is a severe zoonosis involving the activation of inflammatory cells, accompanied by the prominent expressions of proinflammatory cytokines in the host. Semen vaccariae, the seeds of Vaccaria segetalis (Neck.) Garcke. ex Asch. (Caryophyllaceae), is a famous traditional herb that is rich in vaccaria n-butanol extract (VNE). Vaccarin is one major active component of VNE, and it is reported in the treatment of stranguria disease. Hypaphorine is another main active component of VNE and has good anti-inflammatory effect, whereas the potential bioactivity of VNE in trichinellosis treatment is still unknown. MATERIALS AND METHODS: This study was designed to evaluate the potential anthelmintic and anti-inflammatory activity of VNE toward T. spiralis infection. ICR mice were used to assess the effect of VNE on repression larvae and adult worms in vivo. Immunohistochemistry analysis was performed to evaluate the expression levels of IL-1ß, IL-6, TNF-α, and COX-2. RESULTS: Our results showed that VNE could effectively depress the expressions of proinflammatory cytokines, including IL-1ß, IL-6, TNF-α, and COX-2. The adult worms were decreased by 79.53%, while the muscle larvae were diminished by 77.70% as compared to the control. CONCLUSION: These results demonstrated that VNE may be a promising therapeutic agent against the inflammation and diseases caused by T. spiralis infection.


Assuntos
Anti-Inflamatórios/administração & dosagem , Extratos Vegetais/administração & dosagem , Triquinelose/tratamento farmacológico , Vaccaria/química , Animais , Anti-Inflamatórios/análise , Ciclo-Oxigenase 2/genética , Ciclo-Oxigenase 2/imunologia , Humanos , Interleucina-6/genética , Interleucina-6/imunologia , Masculino , Camundongos , Camundongos Endogâmicos ICR , Extratos Vegetais/análise , Trichinella/efeitos dos fármacos , Trichinella/fisiologia , Triquinelose/genética , Triquinelose/imunologia , Triquinelose/parasitologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia
10.
Complement Ther Clin Pract ; 35: 316-322, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31003676

RESUMO

BACKGROUND: and purpose: Adolescent obesity has been increasing globally, and intervention is important. This study examined the effects of auricular acupressure on reducing obesity in adolescents. MATERIALS AND METHODS: Participants were 58 obese adolescents, divided into an experimental (n = 32) and a control group (n = 26). The study design was a randomized controlled trial. Auricular acupressure using vaccaria seeds was administered to the experimental group, while placebo auricular acupressure using vaccaria seeds was administered to the control group. Sessions continued for 8 weeks. Outcome measures included body weight, abdominal circumference, hip circumference, waist-hip ratio, body-mass index, body-fat mass, body-fat percentage, triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, serum glucose, and leptin. RESULTS: Adolescents in the experimental group showed significant improvement in total cholesterol levels and low-density lipoprotein cholesterol levels after 8 weeks compared with those in the control group (p < 0.001). CONCLUSION: Auricular acupressure using vaccaria seeds was effective in decreasing total cholesterol levels and low-density lipoprotein cholesterol levels in adolescents with obesity.


Assuntos
Acupuntura Auricular/métodos , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Obesidade/terapia , Adolescente , Índice de Massa Corporal , Peso Corporal , Criança , Feminino , Humanos , Masculino , Sementes , Vaccaria , Adulto Jovem
11.
Biomed Pharmacother ; 97: 1155-1163, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29136954

RESUMO

Osteoclasts are sole bone-resorbing cells which exert a profound effect on skeletal metabolism. The search for medicines that affect the differentiation and function of osteoclasts is crucial in developing therapies for osteoclast-based diseases. Vaccaria hypaphorine, the main active compound of the traditionally used Chinese herb Vaccaria segetalis, has anti-inflammatory activity. The present study demonstrated for the first time that vaccaria hypaphorine could significantly inhibit the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastic differentiation in vitro and alleviate lipopolysaccharide (LPS)-induced bone loss in vivo. Further study showed that vaccaria hypaphorine decreased osteoclastogenesis in a dose-dependent manner. Furthermore, vaccaria hypaphorine was confirmed to inhibit osteoclasts differentiation at early stage but not at later stage. Pit formation assay and F-actin ring staining showed that vaccaria hypaphorine inhibited the bone-resorbing activity of osteoclasts. Mechanistically, vaccaria hypaphorine impaired RANKL-induced osteoclastogenesis through reduction of extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinase (JNK) and NF-κB p65 phosphorylation. Taken together, our results provided evidences that vaccaria hypaphorine might be considered as potential therapeutic agent for treating osteoclast-based bone loss.


Assuntos
Osteoclastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Extratos Vegetais/farmacologia , Vaccaria/química , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Reabsorção Óssea/tratamento farmacológico , Diferenciação Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Lipopolissacarídeos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Extratos Vegetais/administração & dosagem , Ligante RANK/administração & dosagem , Ligante RANK/metabolismo , Células RAW 264.7
12.
Cell Physiol Biochem ; 42(3): 1227-1239, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28683454

RESUMO

Background /Aims: Accumulating evidence indicates that endothelial inflammation is one of the critical determinants in pathogenesis of atherosclerotic cardiovascular disease. Our previous studies had demonstrated that Vaccariae prevented high glucose or oxidative stress-triggered endothelial dysfunction in vitro. Very little is known about the potential effects of hypaphorine from Vaccariae seed on inflammatory response in endothelial cells. METHODS: In the present study, we evaluated the anti-inflammatory effects of Vaccariae hypaphorine (VH) on lipopolysaccharide (LPS)-challenged endothelial EA.hy926 cells. The inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), monocyte chemoattractant protein 1 (MCP-1) and vascular cellular adhesion molecule-1 (VCAM-1) were measured by real-time PCR (RT-PCR). The expressions of adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), toll-like receptor 4 (TLR4), peroxisome proliferator-activated receptor γ (PPARγ) were detected by Western blotting or immunofluorescence. RESULTS: We showed that LPS stimulated the expressions of TNF-α, IL-1ß, MCP-1, VCAM-1 and TLR4, but attenuated the phosphorylation of AMPK and ACC as well as PPARγ protein levels, which were reversed by VH pretreatment. Moreover, we observed that LPS-upregulated TLR4 protein expressions were inhibited by PPARγ agonist pioglitazone, and the downregulated PPARγ expressions in response to LPS were partially restored by knockdown of TLR4. The negative regulation loop between TLR4 and PPARγ response to LPS was modulated by AMPK agonist AICAR (5-Aminoimidazole-4-carboxamide riboside or acadesine) or A769662. CONCLUSIONS: Taken together, our results suggested that VH ameliorated LPS-induced inflammatory cytokines production in endothelial cells via inhibition of TLR4 and activation of PPARγ, dependent on AMPK signalling pathway.


Assuntos
Proteínas Quinases Ativadas por AMP/imunologia , Anti-Inflamatórios/farmacologia , Células Endoteliais/efeitos dos fármacos , Indóis/farmacologia , PPAR gama/imunologia , Transdução de Sinais/efeitos dos fármacos , Receptor 4 Toll-Like/imunologia , Anti-Inflamatórios/química , Linhagem Celular , Células Endoteliais/imunologia , Humanos , Indóis/química , Lipopolissacarídeos/imunologia , Vaccaria/química
13.
BMC Complement Altern Med ; 17(1): 120, 2017 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-28219355

RESUMO

BACKGROUND: Activation of macrophage is involved in many inflammation diseases. Lipopolysaccharide (LPS) is a powerful inflammatory signal contributing to monocytes/macrophages activation associated with increased proinflammatory cytokines expressions. We recently identified that vaccarin was expected to protect endothelial cells from injury. Hypaphorine was abundantly found in vaccaria semen. However, the potential roles and underlying mechanisms of vaccaria hypaphorine on macrophage inflammation have been poorly defined. METHODS: This study was designed to determine the effects of vaccaria hypaphorine on LPS-mediated inflammation in RAW 264.7 cells. RESULTS: In this study, we demonstrated that vaccaria hypaphorine dramatically ameliorated LPS-induced nitric oxide (NO) release and productions of proinflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), IL-6, IL-10, monocyte chemoattractant protein 1 (MCP-1) and prostaglandin E2 (PGE2) in RAW 264.7 cells. LPS-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were down-regulated by vaccaria hypaphorine. Furthermore, vaccaria hypaphorine retarded LPS-induced phosphorylation of ERK, nuclear factor kappa beta (NFκB), NFκB inhibitor IκBα, and IKKß. Immunofluorescence staining revealed that vaccaria hypaphorine eliminated the nuclear translocation of NFκB in LPS-treated RAW 264.7 cells. CONCLUSION: It was seen that vaccaria hypaphorine counteracted inflammation via inhibition of ERK or/and NFκB signaling pathways. Collectively, we concluded that vaccaria hypaphorine can be served as an anti-inflammatory candidate.


Assuntos
Indóis/farmacologia , Mediadores da Inflamação/metabolismo , Inflamação/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , NF-kappa B/metabolismo , Extratos Vegetais/farmacologia , Vaccaria/química , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Transporte Biológico , Ciclo-Oxigenase 2/metabolismo , Citocinas/metabolismo , Humanos , Quinase I-kappa B/metabolismo , Indóis/uso terapêutico , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Lipopolissacarídeos , Células MCF-7 , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Inibidor de NF-kappaB alfa/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Fosforilação , Fitoterapia , Extratos Vegetais/uso terapêutico , Células RAW 264.7
14.
J Korean Acad Nurs ; 46(2): 249-59, 2016 Apr.
Artigo em Coreano | MEDLINE | ID: mdl-27182021

RESUMO

PURPOSE: The purpose of this study was to examine the effects of auricular acupressure on reducing obesity in adult women with abdominal obesity. METHODS: The study design was a non-equivalent control group pretest-posttest design. Participants were 58 women, aged 20 years or older, assigned to the experimental group (n=30) or control group (n=28). Auricular acupressure using vaccaria seeds was administered to the experimental group. The sessions continued for 8 weeks. Outcome measures included body weight, abdominal circumference, and body mass index, body fat mass, body fat percentage, triglyceride, total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, and serum glucose. RESULTS: Women in the experimental group showed significant decreases in body weight (t=6.19, p<.001), abdominal circumference (t=4.01, p<.001), and body mass index (t=2.58, p=.006) after 8 weeks compared with those in the control group. CONCLUSION: Results show that auricular acupressure using vaccaria seeds was effective in decreasing body weight, abdominal circumference, body mass index, and triglyceride levels in adult women with abdominal obesity.


Assuntos
Acupuntura Auricular , Obesidade Abdominal/terapia , Glicemia/análise , Distribuição da Gordura Corporal , Índice de Massa Corporal , Peso Corporal , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Feminino , Humanos , Sementes/química , Triglicerídeos/sangue , Vaccaria/química , Circunferência da Cintura
15.
Pak J Pharm Sci ; 28(3 Suppl): 1043-8, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26051722

RESUMO

Semen vaccariae, the seeds of Vaccaria segetalis (Neck.) Garcke, is usually used as an important medication for female mammary gland diseases; it has also been used to promote lactation for centuries in China. The purpose of this work was to evaluate the analgesic and anti-inflammatory effects of hydroalcoholic extract from semen vaccariae (HESV) with oral doses of 50, 100 and 200mg/kg•bw in mice and rats. We observed that the HESV could effectively inhibit acetic acid-induced abdominal contraction and could elevate the latency time to thermal stimuli in the hot-plate test in mice. In the xylene-induced ear-swelling test in mice, HESV could suppress the ear swelling. Additionally, HESV could significantly decrease the peritoneal capillary permeability and leukocyte infiltration in mice induced by the intraperitoneal injection of acetic acid. HESV also significantly reduced paw thickness 2-4 hours after the injection of carrageenan in the carrageenan-induced rat paw edema test. This study was the first to demonstrate that the oral administration of HESV might play an important role in the process of analgesia and anti-inflammation, supporting its use for female mammary gland diseases in traditional medicine.


Assuntos
Álcoois/química , Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Edema/prevenção & controle , Dor/prevenção & controle , Extratos Vegetais/farmacologia , Solventes/química , Vaccaria , Ácido Acético , Administração Oral , Analgésicos/administração & dosagem , Analgésicos/química , Analgésicos/isolamento & purificação , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Permeabilidade Capilar/efeitos dos fármacos , Carragenina , Quimiotaxia de Leucócito/efeitos dos fármacos , Modelos Animais de Doenças , Edema/induzido quimicamente , Edema/imunologia , Edema/metabolismo , Temperatura Alta , Camundongos Endogâmicos BALB C , Dor/etiologia , Dor/fisiopatologia , Limiar da Dor/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Sementes , Fatores de Tempo , Vaccaria/química , Xilenos
16.
J Asian Nat Prod Res ; 17(7): 717-23, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25559121

RESUMO

Two new capsaicin analogs, N-(3-methoxy-4-hydroxyphenethyl)-tetracosanamide (1) and N-(3,4-dihydroxyphenethyl)-tetracosanamide (2), along with one new flavonoidal glycoside pinnatifin E (3) were isolated from the ethanolic extract of the seeds of Vaccaria segetalis. Their structures were elucidated on the basis of spectroscopic methods including 1D, 2D NMR, MS, and other spectroscopic techniques, as well as by comparison with the relevant literatures. All compounds were evaluated for their coagulation Factor Xa inhibition activities.


Assuntos
Capsaicina/análogos & derivados , Capsaicina/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Vaccaria/química , Capsaicina/química , Capsaicina/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Fator Xa/efeitos dos fármacos , Flavonoides/química , Flavonoides/farmacologia , Glicosídeos/análise , Glicosídeos/química , Glicosídeos/farmacologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Sementes/química
17.
Rapid Commun Mass Spectrom ; 28(11): 1243-57, 2014 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-24760565

RESUMO

RATIONALE: Seeds of Vaccaria segetalis (Wang-Bu-Liu-Xing in Chinese) are mainly used in traditional Chinese medicine for the treatment of amenorrhea, breast infections, and edema. The study was designed to identify the components and metabolites of Wang-Bu-Liu-Xing. METHODS: A novel methodology combining an adsorbent-separation strategy with analysis by liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/QTOF-MS/MS) was established to identify the components of Wang-Bu-Liu-Xing. The adsorbent-separation technique was applied on macroporous resin (adsorbents). Different concentrations of ethanol (30%, 60%, and 95%), which covered high-to-low polarity ranges, were chosen as the elution solvent, respectively. The QTOF mass spectrometer was operated in negative ion mode with an electrospray ionization source. RESULTS: A total of 52 components were successfully identified in the Wang-Bu-Liu-Xing decoction based on the fragmentation pathways and QTOF high-accuracy mass spectral analysis. To the best of our knowledge, several new saponins were reported for the first time. A total of 20 compounds, which included 10 prototypes and 10 metabolites, were also identified in rat plasma and urine after oral administration of Wang-Bu-Liu-Xing decoction. CONCLUSIONS: An integrated adsorbent-separation strategy is powerful and reliable for global detection and identification of complex components in herbal prescriptions. The components identified in rat biofluids may also provide helpful chemical information for further pharmacology and active mechanism study on this herb.


Assuntos
Cromatografia Líquida/métodos , Medicamentos de Ervas Chinesas/química , Espectrometria de Massas/métodos , Sementes/química , Vaccaria/química , Animais , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/metabolismo , Masculino , Espectrometria de Massas/instrumentação , Plasma/química , Ratos , Ratos Sprague-Dawley , Urina/química
18.
J Pharm Biomed Anal ; 85: 218-30, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23973757

RESUMO

Vaccaria seeds (Wang-Bu-Liu-Xing), a well-known traditional Chinese medicine (TCM), has been used as an emperor herb of many ancient formulas to treat benign prostatic hyperplasia (BPH) in clinic. However, its metabolism and tissue distribution, especially in the target tissue, had not been investigated so far. Based on the hypothesis that the components which exert effect against BPH of Vaccaria seeds would be measureable in target tissue (prostate), in vivo metabolism and tissue distribution of Vaccaria seeds in rats were profiled using a specific and sensitive high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS). As a result, 19 major constituents in the Vaccaria seeds decoction and 19 constituents in rat plasma, feces and tissues after oral administration of Vaccaria seeds decoction were identified. Accurate mass measurement for molecular ions and characteristic fragment ions could represent reliable identification criteria for these compounds. Two prototypes were detected in prostate. An in vitro metabolism analysis of them was studied after incubation with rat intestinal flora and rat liver microsome (RLM) in this paper, which is helpful for further investigation of the potential effect of these two components. The result of this study provided meaningful information for further pharmacology research on Vaccaria seeds.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicina Tradicional Chinesa , Fitoterapia , Extratos Vegetais/metabolismo , Hiperplasia Prostática/tratamento farmacológico , Espectrometria de Massas por Ionização por Electrospray/métodos , Vaccaria , Animais , Modelos Animais de Doenças , Masculino , Microssomos Hepáticos/metabolismo , Hiperplasia Prostática/metabolismo , Ratos , Ratos Sprague-Dawley , Sementes , Distribuição Tecidual
19.
J Ethnopharmacol ; 145(2): 667-9, 2013 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-23202245

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Semen vaccariae, the seeds of Vaccaria Segatalis, is a famous traditional herb for the treatment of stranguria disease, e.g. benign prostatic hyperplasia (BPH). The present study investigated the effect of crude polysaccharide from Semen vaccariae (SVCP) on BPH in mice in order to further understand the efficacy substance of this medicinal plant. MATERIALS AND METHODS: Sixty healthy adult male Kunming mice were randomly divided into five groups with 12 mice for each group: sham-operated group, BPH model group; BPH model group treated with Pule'an at a dose of 1.20 g/kg; BPH model group treated with SVCP at dose levels of 1.67 g/kg and 0.42 g/kg. The drugs were administered orally once a day consecutively for 12 days. The BPH in mice was created by subcutaneous injection of testosterone propionate for 5 mg/(kgd) to the uncastrated mice once a day consecutively for 12 days. The inhibitory effects on BPH of SVCP were evaluated by prostatic index, testicular index and histopathologic examination. RESULTS: Comparing with BPH model group, BPH mice fed with SVCP exhibited significant differences in both the prostatic index and testicular index. On the aspect of histopathology, the SVCP treated BPH mice exhibited similar histological aspects observed in the mice of sham group while the BPH mice exhibited typical features of prostate glandular hyperplasia. CONCLUSIONS: SVCP exhibited a significant inhibitory activity on BPH by reducing the prostatic index, testicular index, and ameliorating the pathomorphology. The results indicated that the polysaccharide should be the main efficacy substance of Semen vaccariae and may contribute, in a large part, to Semen vaccariae's traditional medicinal use for the treatment of stranguria disease.


Assuntos
Fitoterapia , Extratos Vegetais/uso terapêutico , Polissacarídeos/uso terapêutico , Hiperplasia Prostática/tratamento farmacológico , Vaccaria , Animais , Masculino , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Próstata/efeitos dos fármacos , Próstata/patologia , Hiperplasia Prostática/induzido quimicamente , Hiperplasia Prostática/patologia , Sementes , Testículo/efeitos dos fármacos , Testículo/patologia , Propionato de Testosterona
20.
Nat Prod Commun ; 7(9): 1185-6, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23074903

RESUMO

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20-1.16 microg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2"-O-arabinoside. The preferable stir-frying temperature is around 120 degrees C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Vaccaria/química
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