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1.
Molecules ; 26(13)2021 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-34279426

RESUMO

In the present study, an in vitro digestion method has been used to assay the influence of the physiological conditions in the mouth, stomach, and intestine on the stability and activity in different cell models of the main phenolic compounds from Viburnum opulus fresh juice (FJ), phenolic-rich juice (PJ), and the bioavailable fractions (DFJ and DPJ). The data obtained indicate that the V. opulus samples achieved after in vitro digestion had an influence on cellular glucose and lipid metabolism. The bioavailable fraction of both digested juices stimulated glucose uptake and decreased lipid accumulation by L6 myoblasts and HepG2 hepatocytes. Both DFJ and DPJ reduced the secretion of inflammatory cytokines by 3T3-L1 adipocytes: interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Simultaneously, DFJ and DPJ enhanced oxidative stress in MIN6 cells and decreased glucose-stimulated insulin secretion (GSIS). UPLC-MS analysis revealed qualitative and quantitative changes in hydroxycinnamic acids. In particular, the content of chlorogenic acid decreased drastically; its content in the bioavailable fraction was almost 7 times and 30 times lower than in the FJ and PJ, respectively. Our results suggested that although the phenolic compounds of V. opulus juices undergo transformation during digestion, they are still potent antioxidant agents with biological activity.


Assuntos
Antioxidantes/farmacologia , Digestão , Sucos de Frutas e Vegetais/análise , Mioblastos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Viburnum/química , Células Hep G2 , Humanos , Mioblastos/citologia , Mioblastos/metabolismo
2.
Chem Biodivers ; 18(9): e2100404, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34255425

RESUMO

Viburnum species are a group of small trees or shrubs that are of great ornamental and medicinal values. Some of them have been used for a long time both as conventional and ethnic medicine. Viburnum fruits, eaten in fresh and processed forms, have been revealed to contain various health-promoting nutrients. With the increasing research on Viburnum plants, they are considered to be an abundant resource of bioactive natural products possessing diverse pharmacological properties and unique chemical structures, that is powerfully proved by the existence of structurally novel vibsane-type diterpenoids which only occur in Viburnum species, newly discovered lignan constituents with unusual side chains and other noteworthy natural components. This review describes 185 new and 228 known secondary metabolites from Viburnum genus between 2008 and 2020, including their chemical structures, sources and bioactivities, and highlights the corresponding structure-activity relationships.


Assuntos
Antineoplásicos Fitogênicos/química , Antioxidantes/química , Diterpenos/química , Inibidores de Glicosídeo Hidrolases/química , Viburnum/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Benzotiazóis/antagonistas & inibidores , Sobrevivência Celular/efeitos dos fármacos , Diterpenos/metabolismo , Diterpenos/farmacologia , Frutas/química , Frutas/metabolismo , Inibidores de Glicosídeo Hidrolases/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Humanos , Conformação Molecular , Ácidos Sulfônicos/antagonistas & inibidores , Viburnum/metabolismo , alfa-Glucosidases/metabolismo
3.
Food Chem ; 360: 129918, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34051454

RESUMO

The effects of high pressure processing (HPP; 200-600 MPa for 5 or 15 min) and pulsed electric field (PEF; 3 kV/cm, 5-15 kJ/kg) treatment on physicochemical properties (conductivity, pH and total soluble solids content), bioactive compounds (vitamin C, total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) and chlorogenic acid contents), antioxidant capacities (DPPH and CUPRAC assays) and polyphenol oxidase (PPO) activity of cranberrybush purée were evaluated immediately after processing. The results were compared to an untreated purée. According to the results, conductivity increased significantly after PEF (15 kJ/kg) treatment. PEF and HPP treatments resulted in a better retention of bioactive compounds (increase in TPC in the range of ~4-11% and ~10-14% and TFC in the range of ~1-5% and ~6-8% after HPP and PEF, respectively) and antioxidant activity (as measured with CUPRAC method) compared to untreated sample. HPP reduced residual enzyme activity of PPO comparatively better than PEF.


Assuntos
Antioxidantes/química , Ácido Ascórbico/química , Polifenóis/química , Viburnum/química
4.
Molecules ; 26(6)2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33801012

RESUMO

Staphylococcus aureus is still one of the leading causes of both hospital- and community-acquired infections. Due to the very high percentage of drug-resistant strains, the participation of drug-tolerant biofilms in pathological changes, and thus the limited number of effective antibiotics, there is an urgent need to search for alternative methods of prevention or treatment for S. aureus infections. In the present study, biochemically characterized (HPLC/UPLC-QTOF-MS) acetonic, ethanolic, and water extracts from fruits and bark of Viburnum opulus L. were tested in vitro as diet additives that potentially prevent staphylococcal infections. The impacts of V. opulus extracts on sortase A (SrtA) activity (Fluorimetric Assay), staphylococcal protein A (SpA) expression (FITC-labelled specific antibodies), the lipid composition of bacterial cell membranes (LC-MS/MS, GC/MS), and biofilm formation (LIVE/DEAD BacLight) were assessed. The cytotoxicity of V. opulus extracts to the human fibroblast line HFF-1 was also tested (MTT reduction). V. opulus extracts strongly inhibited SrtA activity and SpA expression, caused modifications of S. aureus cell membrane, limited biofilm formation by staphylococci, and were non-cytotoxic. Therefore, they have pro-health potential. Nevertheless, their usefulness as diet supplements that are beneficial for the prevention of staphylococcal infections should be confirmed in animal models in the future.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Fibroblastos , Staphylococcus aureus Resistente à Meticilina/fisiologia , Extratos Vegetais/farmacologia , Viburnum/química , Aminoaciltransferases/biossíntese , Antibacterianos/química , Proteínas de Bactérias/biossíntese , Linhagem Celular , Cisteína Endopeptidases/biossíntese , Fibroblastos/metabolismo , Fibroblastos/microbiologia , Fibroblastos/patologia , Frutas/química , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Humanos , Casca de Planta/química , Extratos Vegetais/química
5.
Phytochemistry ; 187: 112782, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33915418

RESUMO

Six undescribed compounds (1-6) were isolated from the leaves of Viburnum erosum along with four known compounds 7-10. The structures were determined by NMR and MS spectroscopic analyses, and their absolute configurations were established by chemical and spectroscopic methods. Compounds 1-6 were α-glucosidic hydroquinone derivatives with different linear monoterpenoid structures. Compounds 1-10 were also evaluated for their tyrosinase inhibitory activities, and 10 showed potent inhibition of tyrosinase enzyme with IC50 value of 37.9 µM compared to 47.6 µM of the positive control (ß-arbutin).


Assuntos
Viburnum , Arbutina/farmacologia , Glucosídeos , Hidroquinonas/farmacologia , Monofenol Mono-Oxigenase
6.
Molecules ; 26(4)2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33671428

RESUMO

The potential biological activities of Viburnum stellato-tomentosum (VS), a plant mainly found in Costa Rica, have yet to be reported. Supplementation of VS extract for 17 weeks significantly decreased body weight gain, fat weight, fasting glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), and triglyceride levels in high-fat diet (HFD)-fed C57BL/6J mice. The molecular mechanisms underlying the anti-obesity and glucose-lowering effects of VS extract were investigated. VS extract suppressed adipocyte hypertrophy by regulating lipogenesis-related CCAAT/enhancer-binding protein α (C/EBPα) and insulin sensitivity-related peroxisome proliferator-activated receptor γ (Pparg) expression in adipose tissue (AT) and hepatic steatosis by inhibiting C/EBPα and lipid transport-related fatty acid binding protein 4 (FABP4) expression. VS extract enhanced muscular fatty acid ß-oxidation-related AMP-activated protein kinase (AMPK) and PPARα expression with increasing Pparg levels. Furthermore, VS extract contained a much higher content of amentoflavone (AMF) (29.4 mg/g extract) compared to that in other Viburnum species. AMF administration decreased Cebpa and Fabp4 levels in the AT and liver, as well as improved insulin signaling-related insulin receptor substrate 1 (Irs1) and glucose transporter 1 (Glut1) levels in the muscle of HFD-fed mice. This study elucidated the in vivo molecular mechanisms of AMF for the first time. Therefore, VS extract effectively diminished obesity and hyperglycemia by suppressing C/EBPα-mediated lipogenesis in the AT and liver, enhancing PPARα-mediated fatty acid ß-oxidation in muscle, and PPARγ-mediated insulin sensitivity in AT and muscle.


Assuntos
Fármacos Antiobesidade/uso terapêutico , Dieta Hiperlipídica , Hiperglicemia/tratamento farmacológico , Metabolismo dos Lipídeos , Obesidade/tratamento farmacológico , Obesidade/metabolismo , Extratos Vegetais/uso terapêutico , Viburnum/química , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Adipócitos/patologia , Adipogenia/efeitos dos fármacos , Tecido Adiposo Branco/patologia , Animais , Fármacos Antiobesidade/farmacologia , Biflavonoides/farmacologia , Peso Corporal/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Fígado Gorduroso/sangue , Fígado Gorduroso/complicações , Fígado Gorduroso/tratamento farmacológico , Comportamento Alimentar , Glucose/metabolismo , Teste de Tolerância a Glucose , Homeostase/efeitos dos fármacos , Hiperglicemia/sangue , Hiperglicemia/complicações , Hiperglicemia/metabolismo , Hipertrofia , Insulina/metabolismo , Resistência à Insulina , Metabolismo dos Lipídeos/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Obesidade/sangue , Obesidade/complicações , Tamanho do Órgão/efeitos dos fármacos , Oxirredução , Extratos Vegetais/farmacologia , Transdução de Sinais/efeitos dos fármacos
7.
Food Chem Toxicol ; 150: 112053, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33577941

RESUMO

AIM: To investigate the effects of prenatal exposure to AgNPs obtained by green synthesis with Viburnum opulus L. extract on the testis in male offspring rats. MATERIAL AND METHODS: Two different doses of AgNPs (0.8 and 1.5 mg/kg b.w.) and vehicle (PBS) were administered to Wistar female rats on days 3-14 of gestation. At 6 weeks after birth, the ultrastructural changes in correlation with the amount of silver as well as the parameters of oxidative stress, inflammation and cell death mechanisms in the testis of male offspring were evaluated. RESULTS: AgNPs administered during pregnancy crossed the placental and testicular barriers and induced oxidative stress, DNA damage and autophagy as mechanism of cell toxicity. The markers of inflammation and apoptosis decreased after AgNPs exposure while the NFkB activation increased. TEM examination revealed important ultrastructural changes of Sertoli cells, numerous vacuoles and cytoplasmic changes suggestive of the cell's evolution towards necrosis. CONCLUSION: Phytoreduced silver nanoparticles with polyphenols from Viburnum opulus L. fruit extract, administered during the embryological development of the male gonad, have testicular toxic effects in offspring even at 6 weeks after birth.


Assuntos
Morte Celular/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Extratos Vegetais/química , Efeitos Tardios da Exposição Pré-Natal , Prata/toxicidade , Viburnum/química , Animais , Citocinas/genética , Citocinas/metabolismo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamação , Masculino , Nanopartículas Metálicas/química , Estresse Oxidativo/efeitos dos fármacos , Gravidez , Ratos , Prata/química , Testículo/citologia
8.
Food Chem ; 348: 129047, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-33515951

RESUMO

Lipophilic extracts of Viburnum opulus (VOP) and Hippophae rhamnoides (SBP) berry pomace recovered by supercritical CO2 (SFE-CO2) were analysed by UPLC-Q-TOF-MS and GC × GC-TOF for their triacylglycerol, tocopherol, phytosterol and fatty acid composition, while oxidative stability was evaluated by Oxipres and Rancimat methods. SFE-CO2 recovered 16.99% and 26.24% of lipids from SBP and VOP, respectively. Linoleic, linolenic, oleic, palmitic and palmitoleic acids were major in SBP oil, while VOP oil was composed of almost equal amounts of linoleic and oleic acids. Therefore, remarkably higher diversity of triacylglycerols was identified in SBP. The content of ß-sitosterol and α-tocopherol was 359.5-514.5 and 65.38-118.6 mg/100 g, respectively. Hydrocarbons were other quantitatively important lipophilic components, including health beneficial squalene. All extracts improved oxidative stability of mayonnaise. The extracts of berry processing by-products by green extraction method contain valuable bioactive constituents and could be of high interest for applications in functional foods and nutraceuticals.


Assuntos
Dióxido de Carbono/química , Suplementos Nutricionais/análise , Frutas/química , Hippophae/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Viburnum/química , Alimentos , Interações Hidrofóbicas e Hidrofílicas
9.
Bioorg Chem ; 106: 104498, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33272710

RESUMO

Seven new diterpenoids (1-7), including five 7-membered ring vibsane-type diterpenoids, vibsanolide A-E (1-5) and a pair of epimers of 14,15,16,17-tetranorvibsane-type diterpenoids possessing bicyclo[4.2.1]nonane moiety, vibsanolide F-G (6-7), together with twelve known analogues (8-19) were isolated from the crude extracts of the leaves of Viburnum odoratissimum using Small Molecule Accurate Recognition Technology (SMART). These structures including absolute configurations were elucidated by means of comprehensive analyses of spectroscopic data, as well as comparison of the experimental and calculated electronic circular dichroism (ECD) spectra. These compounds were evaluated for their cytotoxic activities against A549 and HepG2 cells by MTT assay. The results showed that compound 2 exhibited potent cytotoxic activity against A549 cells with IC50 value of 1.11 µM. Further staining experiments indicated that 2 could promote apoptosis induction, enhance reactive oxygen species (ROS) level and attenuate mitochondrial membrane potential (MMP) in A549 cells. Taken together, these findings provided new insights into understanding the cytotoxic activity of vibsane-type diterpenoids and it is meaningful to further investigate the application potential of V. odoratissimum.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Diterpenos/farmacologia , Extratos Vegetais/farmacologia , Viburnum/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Diterpenos/química , Diterpenos/isolamento & purificação , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Espécies Reativas de Oxigênio/metabolismo , Relação Estrutura-Atividade
10.
Nat Prod Res ; 35(4): 637-640, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30856004

RESUMO

Vibsane-type diterpenes, the characteristic compounds of Viburnum odoratissimum, exhibited significant cytotoxicity in many cancer cells. To search for the potential target of vibsane-type diterpenes on lung cancer, we combined methods of network pharmacology prediction and experimental verification. 80 active ingredients, 23 potential targets and 39 related pathways were analyzed through constructing the compound-target network and target-pathway network, and the potential target (EGFR) and key pathway (PI3K/Akt) were identified. Vibsanol C, an isolated vibsane-type diterpene with excellent cytotoxicity against lung cancer cells was chosen for further confirmation. Molecular docking study and drug affinity responsive target stability (DARTS) approach further indicated that EGFR is a direct target of Vibsanol C. Moreover, mechanistic studies revealed Vibsanol C might affect PI3K/Akt pathway by Western blot analysis. In conclusion, this study successfully predicted and confirmed the potential target of Vibsane-type diterpenes on lung cancer.


Assuntos
Antineoplásicos/farmacologia , Diterpenos/farmacologia , Viburnum/química , Linhagem Celular Tumoral , Diterpenos/isolamento & purificação , Humanos , Simulação de Acoplamento Molecular , Fosfatidilinositol 3-Quinases/metabolismo , Transdução de Sinais/efeitos dos fármacos
11.
J Ethnopharmacol ; 269: 113742, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33359186

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Viburnum taitoense Hayata has been used as folk medicine by the minority people in Southwestern China for a long history, especially in Guangxi Zhuang Autonomous Region. The minority in Guangxi including Zhuang, Miao and Yao people use the ethanol extract of V. taitoense Hayata to treat the fracture, kill the pain of rheumatism because of its definite therapeutic effects. AIM OF THE STUDY: So far, the scientific investigation of V. taitoense Hayata is done very little. Here, we first prepared the ethyl acetate extract of V. taitoense (EEVt), secondly measured the contents of phenols, flavonoids, and terpenoids in EEVt, and thirdly, the anti-inflammatory and analgesic activities of EEVt were investigated by invitro model of RAW 264.7 cells and invivo models of inflammation and pain in rats and mice. MATERIALS AND METHODS: The contents of phenols, flavonoids, and terpenoids in EEVt were determined by UV spectrophotometry, respectively. The anti-inflammatory effect of EEVt (5, 25, 50, 100, and 200 µg/mL) in vitro was tested by determining its inhibitory effect on the nitric oxide production of RAW264.7 cells activated by lipopolysaccharide (LPS). The anti-inflammatory and analgesic effects of EEVt in vivo were investigated in the following experimental rats and mice models: carrageenan-induced paw edema, corton-oil-induced ear edema, acetic acid writhing test, and formalin pain test. RESULTS: The contents of total phenolic, total flavonoids, and total triterpenoids in V. taitoense were measured to be 3.46 ± 0.04%, 2.38 ± 0.04%, and 14.96 ± 0.17%, respectively. In vitro test showed that EEVt at different tested dosages (5, 25, 50, 100, and 200 µg/mL) had no significant toxicity to RAW264.7 macrophages. At dosages of 37.5 and 75 µg/mL of EEVt significant inhibitory (p < 0.001) on the productions of nitric oxide (NO). High dosage (200 µg/mL) of EEVt displayed highly significant inhibitory (p < 0.001) on the productions of proinflammatory cytokines IL-6, IL-1ß, and TNF-α from the LPS-induced RAW264.7 macrophages. EEVt showed obvious anti-inflammatory activity at different time points after carrageenan injection (p < 0.05) in vivo test, and its anti-inflammatory activity reached the strongest 4 h. Similarly, through the ear swelling test, EEVt (200 mg/kg) showed significant (p < 0.05) anti-inflammatory activity. Besides, formalin and acetic acid writhing experiments also showed that EEVt has significant (p < 0.05) analgesic activity. CONCLUSION: EEVt was confirmed to be definite anti-inflammatory and analgesic effects, and the phytochemicals of EEVt was disclosed to be rich in triterpenoids, which was worthy to be further investigated.


Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Inflamação/tratamento farmacológico , Dor/tratamento farmacológico , Extratos Vegetais/farmacologia , Viburnum/química , Acetatos/química , Analgésicos/uso terapêutico , Animais , Anti-Inflamatórios/uso terapêutico , Comportamento Animal/efeitos dos fármacos , Carragenina/toxicidade , China , Citocinas/antagonistas & inibidores , Modelos Animais de Doenças , Edema/induzido quimicamente , Edema/tratamento farmacológico , Flavonoides/análise , Flavonoides/química , Formaldeído/toxicidade , Inflamação/induzido quimicamente , Lipopolissacarídeos/toxicidade , Medicina Tradicional/métodos , Camundongos , Óxido Nítrico/antagonistas & inibidores , Dor/induzido quimicamente , Fenóis/análise , Fenóis/química , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Células RAW 264.7 , Ratos Sprague-Dawley , Terpenos/análise , Terpenos/química
12.
Nutrients ; 12(11)2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33167421

RESUMO

Viburnum opulus (VO) is a valuable decorative, medicinal, and food plant. This deciduous shrub is found in natural habitats in Europe, Russia, and some regions in North Africa and North Asia. The VO is traditionally used to treat aliments such as cough, colds, tuberculosis, rheumatic aches, ulcers, stomach, and kidney problems, among others. Many of the health-promoting properties of VO are associated with antioxidant activity, which has been demonstrated in both in vitro and in vivo studies. The results of in vitro studies show the antimicrobial potential of VO, especially against Gram-positive bacteria. In cell-based studies, VO demonstrated anti-inflammatory, anti-obesity, anti-diabetic, osteogenic, cardio-protective, and cytoprotective properties. The applicability of VO in the treatment of urinary tract diseases, endometriosis, and some cancers has been confirmed in in vivo studies. The health benefits of VO result from the presence of bioactive components such as phenolic compounds, vitamin C, carotenoids, iridoids, and essential oils. The aim of this review is to present an overview of the botanical characteristics, chemical compositions, including bioactive compounds, and pro-health properties of VO different morphological parts.


Assuntos
Compostos Fitoquímicos/farmacologia , Viburnum/química , Antioxidantes/análise , Fibras na Dieta , Minerais/análise , Nutrientes/análise , Viburnum/anatomia & histologia
13.
Am J Bot ; 107(11): 1527-1541, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33079383

RESUMO

PREMISE: Plant genome size ranges widely, providing many opportunities to examine how genome size variation affects plant form and function. We analyzed trends in chromosome number, genome size, and leaf traits for the woody angiosperm clade Viburnum to examine the evolutionary associations, functional implications, and possible drivers of genome size. METHODS: Chromosome counts and genome size estimates were mapped onto a Viburnum phylogeny to infer the location and frequency of polyploidization events and trends in genome size evolution. Genome size was analyzed with leaf anatomical and physiological data to evaluate the influence of genome size on plant function. RESULTS: We discovered nine independent polyploidization events, two reductions in base chromosome number, and substantial variation in genome size with a slight trend toward genome size reduction in polyploids. We did not find strong relationships between genome size and the functional and morphological traits that have been highlighted at broader phylogenetic scales. CONCLUSIONS: Polyploidization events were sometimes associated with rapid radiations, demonstrating that polyploid lineages can be highly successful. Relationships between genome size and plant physiological function observed at broad phylogenetic scales may be largely irrelevant to the evolutionary dynamics of genome size at smaller scales. The view that plants readily tolerate changes in ploidy and genome size, and often do so, appears to apply to Viburnum.


Assuntos
Radiação , Viburnum , Evolução Molecular , Tamanho do Genoma , Genoma de Planta , Humanos , Filogenia , Poliploidia
14.
Curr Biol ; 30(19): R1078-R1080, 2020 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-33022238

RESUMO

The use of visible colours as honest signals to attract seed-dispersers is a well-known property of fruits. While most of these colours are due to pigments, it has now been discovered that the evergreen Viburnum tinus shrubs display their edible and nutritious fruit with a blue structural colour based on lipid inclusions in the epidermal cells.


Assuntos
Viburnum , Cor , Frutas , Lipídeos , Pigmentação
15.
J Agric Food Chem ; 68(40): 11151-11160, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32902977

RESUMO

Eight previously undescribed lignan glycosides, viburmacrosides A-H (1-8), and seven known analogues (9-15) were isolated from Viburnum macrocephalum f. keteleeri fruits through bioactivity-guided fractionation. Their structures and absolute configurations were elucidated by extensive spectroscopic analyses and chemical evidence. Using the well-recognized carbohydrate-hydrolyzing enzymes α-amylase and α-glucosidase, as well as the promising protein tyrosine phosphatase 1B (PTP1B), as inhibitory targets, all isolated compounds were tested for their antidiabetic potential in vitro. Compound 4 displayed potent inhibitory activities with IC50 values of 9.9 ± 0.6 and 8.9 ± 0.5 µM against α-glucosidase and PTP1B, respectively. The enzymatic kinetics results suggested that compound 4 competitively inhibited α-glucosidase while it suppressed α-amylase and PTP1B in the mixed-type manner. These findings supported that V. macrocephalum f. keteleeri fruits may be a new functional food resource with antidiabetic potential.


Assuntos
Inibidores Enzimáticos/química , Lignanas/química , Extratos Vegetais/química , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , Viburnum/química , alfa-Amilases/antagonistas & inibidores , Frutas/química , Inibidores de Glicosídeo Hidrolases/química , Humanos , Hipoglicemiantes/química , Cinética , Estrutura Molecular , Proteína Tirosina Fosfatase não Receptora Tipo 1/química , alfa-Amilases/química , alfa-Glucosidases/química
16.
Curr Biol ; 30(19): 3804-3810.e2, 2020 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-32763166

RESUMO

Viburnum tinus is an evergreen shrub that is native to the Mediterranean region but cultivated widely in Europe and around the world. It produces ripe metallic blue fruits throughout winter [1]. Despite its limited fleshy pulp [2], its high lipid content [3] makes it a valuable resource to the small birds [4] that act as its seed-dispersers [5]. Here, we find that the metallic blue appearance of the fruits is produced by globular lipid inclusions arranged in a disordered multilayer structure. This structure is embedded in the cell walls of the epicarp and underlaid with a dark layer of anthocyanin pigments. The presence of such large, organized lipid aggregates in plant cell walls represents a new mechanism for structural coloration and may serve as an honest signal of nutritional content.


Assuntos
Antocianinas/metabolismo , Metabolismo dos Lipídeos/fisiologia , Viburnum/metabolismo , Antocianinas/fisiologia , Cor , Frutas/química , Frutas/metabolismo , Lipídeos/química , Pigmentação/fisiologia , Dispersão de Sementes/fisiologia , Relação Estrutura-Atividade , Viburnum/genética
17.
Nutrients ; 12(7)2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32640537

RESUMO

Viburnum opulus L. fruit is a rich source of phenolic compounds that may be involved in the prevention of metabolic diseases. The purpose of this study was to determine the effects of Viburnum opulus fresh juice (FJ) and juice purified by solid-phase extraction (PJ) on the adipogenesis process with murine 3T3-L1 preadipocyte cell line and pancreatic lipase activity in triolein emulsion, as well as their phenolic profiles by UPLC/Q-TOF-MS. Decrease of lipids and triacylglycerol accumulation in differentiated 3T3-L1 cells were in concordance with downregulation of the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPß/α), and sterol regulatory element-binding protein 1c (SREBP-1c). Furthermore, regulation of PPARγ-mediated ß-lactamase expression by V. opulus components in reporter gene assay, as well as their binding affinity to ligand-binding domain of PPARγ, were tested. In addition, the levels of enzymes involved in lipid metabolism, like fatty acid synthase (FAS) or acetyl-CoA carboxylase (ACC), were decreased, along with inflammatory cytokines, like tumor necrosis factorα (TNFα), interleukin-6 (Il-6) and leptin. Moreover, FJ and PJ were able to inhibit pancreatic lipase, which potentially could reduce the fat absorption from the intestinal lumen and the storage of body fat in the adipose tissues. Thirty-two phenolic compounds with chlorogenic acid as the dominant compound were identified in PJ which revealed significant biological activity. These data contribute to elucidate V. opulus juice phenolic compounds' molecular mechanism in adipogenesis regulation in 3T3-L1 cells and dietary fat lipolysis.


Assuntos
Adipogenia/efeitos dos fármacos , Lipase/antagonistas & inibidores , Fenóis/farmacologia , Compostos Fitoquímicos/farmacologia , Viburnum/química , Células 3T3-L1 , Animais , Sobrevivência Celular/efeitos dos fármacos , Sucos de Frutas e Vegetais/análise , Camundongos , Estresse Oxidativo/efeitos dos fármacos , PPAR gama/metabolismo
18.
Int J Mol Sci ; 21(14)2020 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-32664580

RESUMO

Bone mass loss occurs with a decrease in osteoblast proliferation and differentiation, or the enhancement of bone resorption, which further leads to the impairment of bone mineral density and increase in bone fracture. Recent studies suggest that some phenolic compounds found in food play positive role in bone metabolism. High content of phenolic compounds with potential beneficial effects on bone metabolism have been identified in the Viburnum opulus fruit. The aim of the study was to determine the influence of V. opulus fresh juice (FJ) and juice purified by solid phase extraction (PJ) on osteogenesis processes with osteosarcoma Saos-2 cell lines. V. opulus purified juice revealed stronger potential as an inducer of Saos-2 osteogenic differentiation. Saos-2 cells matrix mineralization was evaluated with alkaline phosphatase (ALP) activity measurement and alizarin red S staining. Gene expression analysis showed the elevation of the mRNA levels of Runt-related transcription factor 2 (RUNX2), ALP, collagen type 1 and osteonectin, whereas the nuclear factor-κB ligand and osteoprotegerin ratio (RANKL/OPG) decreased. Furthermore, V. opulus was able to diminish the secretion of pro-inflammatory cytokines Il6 and TNFα, however had no effect on vascular endothelial growth factor (VEGF). It decreased intracellular oxidative stress and induced DNA repair, but had no effect on the growth inhibition of lactic acid beneficial microorganisms.


Assuntos
Conservadores da Densidade Óssea/farmacologia , Neoplasias Ósseas/patologia , Sucos de Frutas e Vegetais/análise , Osteogênese/efeitos dos fármacos , Osteossarcoma/patologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Viburnum/química , Bactérias/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Reparo do DNA/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Microbioma Gastrointestinal/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Interleucina-6/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/enzimologia , Proteínas de Neoplasias/biossíntese , Proteínas de Neoplasias/genética , Osteogênese/genética , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
19.
Mycologia ; 112(3): 616-632, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32374657

RESUMO

The phylogeny and taxonomy of powdery mildew on Viburnum species is evaluated and discussed. Morphological and phylogenetic analyses revealed two new species and demonstrated that Erysiphe hedwigii and E. viburni should be reduced to synonymy and are referred to herein as E. viburni. The two new species, E. viburniphila and E. pseudoviburni, previously hidden under E. viburni (including E. hedwigii), is described on the basis of European, North American, and East Asian powdery mildew collections on Viburnum edule, V. tinus, V. odoratissimum var. awabuki, and V. sieboldii. The sexual morph of E. viburniphila is similar to that of E. viburni; however, morphological differences exist in their asexual morphs. Analyses of sequences from the internal transcribed spacer (ITS) and 28S genomic regions of Erysiphe species obtained on Viburnum species (and other closely allied Eryisphe species) throughout the world reveled that E. viburniphila and E. pseudoviburni are in two different monophyletic groups that are separate from all other Erysiphe species. Erysiphe hedwigii and E. viburni on Viburnum species have often been recognized as separate species based on morphological differences in the size of their chasmothecia and the number of chasmothecial appendages. Taxonomic conclusions based on these morphological distinctions within these species are unreliable (these characters are rather variable and often have overlapping ranges). The present phylogenetic analyses suggest that E. hedwigii has to be reduced to synonymy with E. viburni. To fix the application of the species names E. hedwigii and E. viburni, epitypes have been designated for these taxa with ex-epitype sequences. Additionally, the Asian species E. miranda is phylogenetically confirmed as a species of its own, described in detail and discussed.


Assuntos
DNA Fúngico/genética , Erysiphe/classificação , Erysiphe/citologia , Erysiphe/genética , Filogenia , Viburnum/parasitologia , Doenças das Plantas
20.
Bol. latinoam. Caribe plantas med. aromát ; 19(3): 321-333, mayo 2020. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1116432

RESUMO

In this study the in vitro investigation of the inhibitory effect of ethanol extract of Viburnum opulus L. bark sample on Streptococcus mutans planctonic cells and biofilm has been intended. A Scanning electron microscopy analysis has been performed in order to investigate the inhibitory effect of the extract on Streptococcus mutans biofilms. Furthermore, the Exopolysaccharide and dextran production of this bacteria have been identified in the presence of the extract. It has been found out that the bark extract with the concentration of 2,5 mg/mL is able to inhibit more than 50% of the cells in the different times development phases. According to this, the exopolymeric matrix on the biofilm surface disperses and the Exopolysaccharide and dextran production get lowered in the presence of bark extract compared to the control group. It is considered that this extract can be used as an alternative approach for the new chemotherapeutic strategies against tooth decay.


En este estudio se investigó el efecto inhibitorio in vitro del extracto de etanólico de una muestra de corteza de Viburnum opulus L. en biopelículas de células planctónicas de Streptococcus mutans. Se realizó un análisis de microscopía electrónica de barrido para investigar el efecto inhibitorio del extracto sobre las biopelículas de Streptococcus mutans. Además, se identificó la producción de exopolisacárido y dextrano de esta bacteria en presencia del extracto. Se descubrió que el extracto de corteza con una concentración de 2,5 mg/ml inhibió más del 50% de las células en las diferentes fases de desarrollo. Consecuentemente, la matriz exopolimérica en la superficie de la biopelícula se dispersa y la producción de exopolisacárido y dextrano se reduce en presencia de extracto de corteza en comparación con el grupo de control. Se sugiere que este extracto puede ser usado como un enfoque alternativo para las nuevas estrategias quimioterapéuticas contra la carie dental.


Assuntos
Streptococcus mutans/efeitos dos fármacos , Extratos Vegetais/farmacologia , Viburnum opulus/farmacologia , Viburnum/química , Polissacarídeos Bacterianos/análise , Streptococcus mutans/metabolismo , Técnicas In Vitro , Microscopia Eletrônica de Varredura , Dextranos/análise , Biofilmes/efeitos dos fármacos , Etanol , Incrustação Biológica
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