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1.
BMJ Case Rep ; 15(1)2022 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-35027389

RESUMO

In clinical practice, the finding of an elevated serum B12 concentration is often the consequence of supplementation with B12 in either oral form or injections. Also, elevated serum B12 may be associated with underlying disorders, like liver diseases or a (haematologic) malignancy. Only a few studies have shown that it may also be the consequence of complex formation of B12-vitamin binding proteins with immunoglobulins, the so-called macro-B12 We describe a young woman who previously was diagnosed with B12 deficiency, and in whom, after cessation of B12 injection treatment, neurologic symptoms re-appeared, and despite this, repeatedly elevated serum B12 concentrations above the upper limit of the assay were found. We demonstrated that this was caused by the presence of macro-B12, which not only resulted in erroneous and longstanding elevated serum B12, but also masked her underlying B12 deficiency.


Assuntos
Deficiência de Vitamina B 12 , Feminino , Humanos , Vitamina B 12/uso terapêutico , Deficiência de Vitamina B 12/diagnóstico , Deficiência de Vitamina B 12/tratamento farmacológico , Vitaminas
2.
Rev Alerg Mex ; 69 Suppl 1: s46-s54, 2022.
Artigo em Espanhol | MEDLINE | ID: mdl-34998310

RESUMO

An increasing number of studies are analyzing the relationship between serum vitamin D levels and the development of sensitization and allergic diseases in genetically predisposed individuals, as well as the impact of vitamin D supplementation. This article reviews the literature on this subject. Clinical trials, meta-analyses and systematic reviews consulted in PubMed, EMBASE, Scopus, Ovid, Wiley Online Library, Springer, Cochrane and manual resources were included, with the keywords: vitamin D, 25 hydroxyvitamin D, cholecalciferol, asthma, rhinitis, allergy, 25-OH-D, 1,25 hydroxyvitamin D, supplementation. The results show a positive linear trend, however, differ. We should keep in mind that in the studies there is heterogeneity of population groups and associated factors, which may modify such studies. It is necessary to increase research to clarify this relationship and to have successful interventions from the patient's approach to the strengthening of pharmacological and immunological treatment of allergic patients with these diseases.


Cada vez son más los trabajos que analizan la relación de los niveles séricos de vitamina D y el desarrollo de sensibilizaciones y enfermedades alérgicas en los individuos con predisposición genética, así como el impacto de su suplementación. El presente artículo efectúa una revisión de la literatura acerca de este tema. Se incluyeron ensayos clínicos, metaanálisis y revisiones sistemáticas consultadas en PubMed, EMBASE, Scopus, Ovid, Wiley Online Library, Springer, Cochrane y recursos manuales, con las palabras clave: vitamina D, 25 hidroxivitamina D, colecalciferol, asma, rinitis, alergia, 25-OH-D, 1,25 hidroxivitamina D, suplementación. Los resultados muestran una tendencia lineal positiva; sin embargo, algunos difieren. Debemos tener en mente que en los estudios existe heterogeneidad de los grupos poblacionales y los factores asociados, lo que puede modificarlos. Es necesario incrementar las investigaciones para clarificar esta relación y tener intervenciones exitosas desde el abordaje del paciente hasta el fortalecimiento del tratamiento farmacológico e inmunológico de los pacientes alérgicos con estas enfermedades.


Assuntos
Asma , Hipersensibilidade , Deficiência de Vitamina D , Asma/tratamento farmacológico , Colecalciferol , Humanos , Hipersensibilidade/epidemiologia , Vitamina D , Deficiência de Vitamina D/tratamento farmacológico , Deficiência de Vitamina D/epidemiologia , Vitaminas/uso terapêutico
3.
BMC Infect Dis ; 22(1): 52, 2022 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-35026987

RESUMO

BACKGROUND: Invasive pneumococcal disease is a major cause of infant morbidity and death worldwide. Vitamin D promotes anti-pneumococcal immune responses in vitro, but whether improvements in infant vitamin D status modify risks of nasal pneumococcal acquisition in early life is not known. METHODS: This is a secondary analysis of data collected in a trial cohort in Dhaka, Bangladesh. Acute respiratory infection (ARI) surveillance was conducted from 0 to 6 months of age among 1060 infants of women randomized to one of four pre/post-partum vitamin D dose combinations or placebo. Nasal swab samples were collected based on standardized ARI criteria, and pneumococcal DNA quantified by qPCR. Hazards ratios of pneumococcal acquisition and carriage dynamics were estimated using interval-censored survival and multi-state modelling. RESULTS: Pneumococcal carriage was detected at least once in 90% of infants by 6 months of age; overall, 69% of swabs were positive (2616/3792). There were no differences between any vitamin D group and placebo in the hazards of pneumococcal acquisition, carriage dynamics, or carriage density (p > 0.05 for all comparisons). CONCLUSION: Despite in vitro data suggesting that vitamin D promoted immune responses against pneumococcus, improvements in postnatal vitamin D status did not reduce the rate, alter age of onset, or change dynamics of nasal pneumococcal colonization in early infancy. Trial registration Registered in ClinicalTrials.gov with the registration number of NCT02388516 and first posted on March 17, 2015.


Assuntos
Infecções Pneumocócicas , Streptococcus pneumoniae , Bangladesh/epidemiologia , Portador Sadio/epidemiologia , Suplementos Nutricionais , Feminino , Humanos , Lactente , Nasofaringe , Infecções Pneumocócicas/epidemiologia , Infecções Pneumocócicas/prevenção & controle , Vacinas Pneumocócicas , Vitamina D , Vitaminas
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 268: 120706, 2022 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-34915228

RESUMO

Cobalt as a transition metal ion is a biologically essential trace element, and plays an important role in various biological systems. The silicon nanoparticles (SiNPs) / gold nanoparticles (AuNPs) composite as a simple and efficient fluorescent probe was developed to detect Co2+ and vitamin B12 (VB12) based on the selective aggregation and inner filter effect (IFE). The green-emitting SiNPs were synthesized by one-pot hydrothermal method, and the AuNPs were synthesized and modified with thioglycolic acid and cetyltrimethylammonium bromide. The fluorescent probe was fabricated by simple mixing the SiNPs and AuNPs together. In the presence of Co2+/VB12, AuNPs are selectively aggregated, which results in the enhancement of the local surface plasmon resonance absorption centered at 520 nm and the green fluorescence of SiNPs is significantly quenched via IFE. The fluorescence quenching efficiency of the probe is linearly proportional to the concentration of Co2+ in the range of 0.1-80 µM with a low detection limit of 60 nM, which is far lower than the guideline value of Co2+ in drinking water (1.7 µM). For vitamin B12 (VB12), its linear relationship is in the range of 0.1-100 µM, and the limit of detection is 69 nM. Furthermore, the proposed method shows good selectivity for the detection of Co2+ and VB12, and does not need sophisticated pretreatment, only through simple filter. It has been applied in actual environmental water samples and drug tablets with satisfactory results.


Assuntos
Nanopartículas Metálicas , Pontos Quânticos , Fluorescência , Corantes Fluorescentes , Ouro , Limite de Detecção , Silício , Vitamina B 12 , Vitaminas
5.
Food Chem ; 368: 130745, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34404004

RESUMO

The subject of the present research is the evaluation of health-promoting properties caused by the presence of some vitamins as well as the antioxidative potential of the honeybee drone brood homogenate (DBH). The study used 139 homogenate samples obtained from various apiaries and collected over 3 years, three times during each beekeeping season. Samples differed in terms of varroa infestation, stage of brood development, location of the apiary, and the degree of environmental contamination. The content of ascorbic acid, α-tocopherol, all-trans-retinol, and coenzyme Q10 in the tested samples was determined through the application of HPLC/DAD/UV and LC/QQQ/MS methods. The antioxidant potential of samples was assessed using the Folin-Ciocalteu and DPPH methods.


Assuntos
Antioxidantes , Varroidae , Animais , Criação de Abelhas , Abelhas , Fenóis , Vitaminas
6.
Nanoscale ; 14(2): 506-514, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34913938

RESUMO

Lipid nanoemulsions are promising nanomaterials for drug delivery applications in food, pharmaceutical and cosmetic industries. Despite the noteworthy commercial interest, little is known about their supramolecular organization, especially about how such multicomponent formulations interact with cell membranes. In the present work, coarse-grained molecular dynamics simulations have been employed to study the self-assembly of a 15-component lipid nanoemulsion droplet containing vitamins A and E for skin delivery. Our results display aspects of the unique "onion-like" agglomeration between the chemical constituents in the different layers of the lipid nanodroplet. Vitamin E molecules are more concentrated in the center of the droplet together with other hydrophobic constituents such as the triglycerides with long tails. On the other hand, vitamin A occupies an intermediate layer between the core and the co-emulsifier surface of the nanodroplet, together with lecithin phospholipids. Coarse-grained molecular dynamics simulations were also performed to provide insight into the first steps involved in absorption and penetration of the nanodroplet through skin membrane models, representing an intracellular (hair follicle infundibulum) and intercellular pathway (stratum corneum) through the skin. Our data provide a first view on the complex organization of commercial nanoemulsion and its interaction with skin membranes. We expect our results to open the way towards the rational design of such nanomaterials.


Assuntos
Absorção Cutânea , Vitaminas , Sistemas de Liberação de Medicamentos , Emulsões , Pele/metabolismo
7.
Environ Res ; 203: 111929, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34428453

RESUMO

BACKGROUND: Solar ultraviolet radiation (UV) is a critical environmental factor for dermal conversion of vitamin D, which is suggested to support reproductive health. However, current epidemiological studies have reported conflicting results on the associations between vitamin D levels and ovarian reserve. Further, few studies have considered UV exposure and reproductive aging, which is closely related to declined ovarian reserve. OBJECTIVES: We sought to examine the associations of long-term UV exposure and age at natural menopause in a large, nationwide, prospective cohort. METHODS: Participants in the Nurses' Health Study II (NHS II) who were premenopausal at age 40 were included and followed through 2015. Erythemal UV radiation from a high-resolution geospatial model was linked to the participants' residential histories. Early-life UV was estimated using the reported state of residence at birth, age 15, and age 30. We used time-varying Cox proportional hazards models to estimate the hazard ratio (HR) and 95% confidence intervals (CIs) for natural menopause, adjusting for potential confounders and predictors of menopause. RESULTS: A total of 63,801 women reported natural menopause across the 1,051,185 person-years of follow-up among 105,631 eligible participants. We found very modest associations with delayed menopause for long-term UV exposure (adjusted HR comparing highest to lowest quartile of cumulative average UV: 0.96, 95% CI: 0.94, 0.99). There was a suggestive inverse association between UV at age 30 with menopause (adjusted HR comparing highest to lowest quartile: 0.97, 95% CI: 0.95, 1.00) but not with UV at birth and age 15. CONCLUSIONS: Solar UV exposure in adulthood was modestly associated with later onset of menopause. Although consistent with previous findings on vitamin D intake and menopause in the same population, these weak associations found in this study may not be of clinical relevance.


Assuntos
Menopausa , Raios Ultravioleta , Adolescente , Adulto , Estudos de Coortes , Feminino , Humanos , Recém-Nascido , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Risco , Raios Ultravioleta/efeitos adversos , Vitaminas
8.
Food Chem ; 370: 131021, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34536784

RESUMO

Some bioactive compounds found in pulses (phytates, saponins, tannins) display antinutritional properties and interfere with fat-soluble vitamin bioavailability (i.e., bioaccessibility and intestinal uptake). As canned chickpeas are consumed widely, our aim was to optimize the chickpea canning process and assess whether this optimization influences fat-soluble vitamin bioavailability. Different conditions during soaking and blanching were studied, as was a step involving prior germination. Proteins, lipids, fibers, vitamin E, lutein, 5-methyl-tetrahydro-folate, magnesium, iron, phytates, saponins and tannins were quantified. Bioaccessibility and intestinal uptake of vitamin D and K were assessed using in vitro digestion and Caco-2 cells, respectively. Significant reductions of phytate, saponin and tannin contents (-16 to -44%), but also of folate content (up to -97%) were observed under optimized canning conditions compared with the control. However, bioaccessibility and cellular uptake of vitamin D and K remained unaffected after in vitro digestion of test meals containing control or optimized canned chickpeas.


Assuntos
Ácido Fítico , Vitaminas , Disponibilidade Biológica , Células CACO-2 , Digestão , Humanos , Taninos
9.
Food Chem ; 369: 130900, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34496317

RESUMO

Thermal process is the most important way of treating foods. Heat energy inputted into the natural food system induces the depolymerization of multi-scale structures of matrix, and causes the intramolecular and intermolecular interactions of different nutrients. It attacks and breaks the original polymeric molecule structures and the functional properties of macronutrients such as carbohydrates, proteins and lipids. Micronutrients such as vitamins and other novel functional ingredients are also thermally converted. The heat-induced conversions of nutrients are slightly or totally with discrepancy in simple-, simulated- and real-food systems, respectively. Thus, this review aims to extensively summarize the heat-induced structural characteristics, thermal conversion pathways and pyrolysis mechanism of nutrients both in simple and complex food matrices. The structural change of each nutrient and its thermal reaction kinetics depend on the molecule structure and polymeric characteristic of the unit substances in the system.


Assuntos
Temperatura Alta , Nutrientes , Alimentos , Micronutrientes , Estrutura Molecular , Vitaminas
10.
Food Chem ; 372: 131325, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34649031

RESUMO

Vitamin d-fortified bread has been proposed as a strategy to increase the average daily intake and serum status of this nutrient. This review aimed to bring together the different types of scientific articles on vitamin d-fortified bread. The databases used for the research were PUBMED, WEB of SCIENCE and SCOPUS; all original indexed studies written in English, published between January 2000 and March 2021, were considered. Three important points were identified: i) theoretical models of fortification; ii) stability, bioaccessibility, and bioavailability studies of vitamin d-fortified breads; and iii) clinical effects of vitamin d-fortified breads. This review showed that vitamin d-fortified bread is a promising vehicle for fortification strategy effects, leading to increased serum concentrations of 25(OH)D and decreased parathyroid hormone. However, further studies are needed to elucidate the effects and effectiveness of this fortification strategy in the prevention/treatment of vitamin D deficiency.


Assuntos
Pão , Vitamina D , Colecalciferol , Alimentos Fortificados , Vitaminas
11.
Food Chem ; 371: 131050, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34537615

RESUMO

Vitamin B3, derived primarily from plant sources, is an essential nutrient for humans. Torreya grandis is rich in vitamin B3, however, the mechanism underlying the biosynthesis and regulation of vitamin B3 in T. grandis remains unclear. A systematic transcriptomic investigation was thus conducted to identify the gene expression pattern of vitamin B3 biosynthesis in 10 T. grandis cultivars. The findings suggest that biosynthesis occurs mainly via the aspartate pathway. Expression and correlation analyses indicate that aspartate oxidase (AOX) and quinolinate synthase (QS) may play important roles in vitamin B3 accumulation. Furthermore, co-expression network and ethephon treatments indicate that the ethylene response factor (ERF) may be involved in the regulation of vitamin B3 biosynthesis in T. grandis nuts. Our findings not only help to elucidate the biosynthesis of vitamin B3, but also provide valuable resource material for future genomic research and molecular-assisted breeding to develop genotypes with higher vitamin B3 levels.


Assuntos
Niacinamida , Nozes , Taxaceae , Etilenos , Expressão Gênica , Regulação da Expressão Gênica de Plantas , Humanos , Nozes/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Vitaminas
12.
Int J Cancer ; 150(2): 303-307, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34449871

RESUMO

Epidemiological evidence is consistent with a protective effect of vitamin D against colorectal cancer (CRC), but the observed strong associations are open to confounders and potential reverse causation. Previous Mendelian randomisation (MR) studies were limited by poor genetic instruments and inadequate statistical power. Moreover, whether genetically higher CRC risk can influence vitamin D level, namely the reverse causation, still remains unknown. Herein, we report the first bidirectional MR study. We employed 110 newly identified genetic variants as proxies for vitamin D to obtain unconfounded effect estimates on CRC risk in 26 397 CRC cases and 41 481 controls of European ancestry. To test for reserve causation, we estimated effects of 115 CRC-risk variants on vitamin D level among 417 580 participants from the UK Biobank. The causal association was estimated using the random-effect inverse-variance weighted (IVW) method. We found no significant causal effect of vitamin D on CRC risk [IVW estimate odds ratio: 0.97, 95% confidence interval (CI) = 0.88-1.07, P = .565]. Similarly, no significant reverse causal association was identified between genetically increased CRC risk and vitamin D levels (IVW estimate ß: -0.002, 95% CI = -0.008 to 0.004, P = .543). Stratified analysis by tumour sites did not identify significant causal associations in either direction between vitamin D and colon or rectal cancer. Despite the improved statistical power of this study, we found no evidence of causal association of either direction between circulating vitamin D and CRC risk. Significant associations reported by observational studies may be primarily driven by unidentified confounders.


Assuntos
Neoplasias Colorretais/epidemiologia , Estudo de Associação Genômica Ampla , Análise da Randomização Mendeliana/estatística & dados numéricos , Polimorfismo de Nucleotídeo Único , Vitamina D/sangue , Vitaminas/sangue , Estudos de Casos e Controles , Causalidade , Neoplasias Colorretais/sangue , Neoplasias Colorretais/genética , Seguimentos , Humanos , Prognóstico , Fatores de Risco , Reino Unido/epidemiologia
13.
Methods Mol Biol ; 2343: 345-360, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34473336

RESUMO

Aging results in loss of muscle mass and strength, which are linked to development of metabolic disorders such as insulin resistance, obesity, and type 2 diabetes mellitus. A number of studies have now shown that these effects can be ameliorated by dietary supplementation with natural products such as vitamins, omega-3 fatty acids, and protein and by physical activities such as aerobic and resistance exercise. Here, we present a protocol for setting up a trial to test the effects of vitamin D and omega-3 fatty acid supplementation and resistance exercise on various anthropometric and molecular measurements in middle-aged females.


Assuntos
Treinamento de Força , Diabetes Mellitus Tipo 2 , Suplementos Nutricionais , Exercício Físico , Feminino , Humanos , Pessoa de Meia-Idade , Vitaminas/farmacologia
14.
Nutrients ; 13(12)2021 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-34960016

RESUMO

BACKGROUND: Inadequate vitamin D levels may increase the risk of caries during childhood. The purpose of this study was to investigate the association between 25-hydroxyvitamin D (25(OH)D) status and severe early childhood caries (S-ECC) in preschool children. METHODS: Data were obtained from children <72 months of age in two case-control studies in Winnipeg, Manitoba and Richmond, Virginia. Serum analysis assessed 25(OH)D, calcium and parathyroid concentrations. Data on demographics, dental history and oral hygiene were obtained via questionnaires. Bivariate and multiple logistic regression analyses were performed to assess the relationships between demographic and biological variables and S-ECC. A p-value of ≤0.05 was significant. RESULTS: Data were available for 200 children with S-ECC and 144 caries-free controls. Children with S-ECC had significantly lower 25(OH)D levels than those who were caries-free (p < 0.001), and children with deficient 25(OH)D levels were 10 times more likely to have S-ECC (p < 0.001). Multiple logistic regression revealed that having higher 25(OH)D and calcium concentrations (p = 0.019 and p < 0.0001, respectively), as well as being breastfed in infancy (p < 0.001), were significantly and independently associated with lower odds of S-ECC, while dental insurance (p = 0.006) was associated with higher odds of S-ECC. CONCLUSIONS: This study provides additional evidence of an association between nutritional status, specifically vitamin D and calcium levels, and S-ECC.


Assuntos
Cárie Dentária/epidemiologia , Cárie Dentária/prevenção & controle , Suplementos Nutricionais , Vitamina D/análogos & derivados , Vitamina D/farmacologia , Vitaminas/farmacologia , Canadá/epidemiologia , Pré-Escolar , Humanos , Estados Unidos/epidemiologia , Vitamina D/sangue
15.
BMJ Case Rep ; 14(12)2021 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-34969788

RESUMO

Vitamin D-resistant rickets shows the resistance to vitamin D (Vit-D) therapy, which traditionally works well in cases with deficiency rickets. The signs start appearing as early as in the first month of life and are characterised by the defective mineralisation at the ends of cartilage and bones despite having normal Vit-D levels in the serum. This case report highlights the dental and maxillofacial manifestations in a 3-year-old girl diagnosed with pseudo-Vit-D deficiency rickets. The report also highlights the variations in the dental manifestations of the condition reported in the literature.


Assuntos
Raquitismo Hipofosfatêmico Familiar , Raquitismo , Deficiência de Vitamina D , Osso e Ossos , Criança , Pré-Escolar , Raquitismo Hipofosfatêmico Familiar/complicações , Feminino , Humanos , Raquitismo/diagnóstico , Raquitismo/etiologia , Vitamina D , Vitaminas
16.
Nutrients ; 13(12)2021 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-34959934

RESUMO

BACKGROUND: A balanced diet is an important lifestyle component and has been associated with a reduced risk of chronic diseases. OBJECTIVES: To assess dietary intake of adult residents in Luxembourg taking part in two population-based cross-sectional studies (ORISCAV-LUX, 2007-2008 and ORISCAV-LUX 2, 2016-2017). METHODS: Dietary intake of the study participants (1242 in 2007/08 and 1326 in 2016/17), 25-69 years old, were evaluated using food-frequency questionnaires (134 items in 2007/2008 and 174 items in 2016/2017) according to the French ANSES-CIQUAL food composition database. Both food-group- and nutrient-based analyses were conducted. RESULTS: Dietary patterns in ORISCAV-LUX 2, 2016-2017, were characterized by an increase in the estimated marginal means (EMM) of the intake of energy, total fat, saturated fatty acids, alcohol, and decreased EMM of total carbohydrates, magnesium, and calcium compared to 2007/08. We also observed an increased EMM of the intake of protein-rich food items and ready-to-eat foods/fast foods, together with a decreased intake of grains, dairy products, and vegetables (all p-values <0.05, linear mixed models). The intake of most micronutrients was stable or slightly increased in ORISCAV-LUX 2 vs. ORISCAV-LUX, except for the drop in magnesium and calcium, and generally met recommendations, in particular, EFSA population reference intakes (PRI), except for vitamin D. CONCLUSIONS: Though most micronutrient recommendations were met, nutrient consumption in terms of high energy, total fat, and sodium, as well as low carbohydrates, were not aligned with recommendations for balanced eating.


Assuntos
Estudos Transversais , Ingestão de Alimentos , Comportamento Alimentar , Vida Independente , Vigilância da População/métodos , Inquéritos e Questionários , Adulto , Idoso , Dieta Saudável , Ingestão de Energia , Feminino , Humanos , Luxemburgo , Masculino , Micronutrientes , Pessoa de Meia-Idade , Minerais , Vitaminas
17.
Nutrients ; 13(12)2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34959778

RESUMO

Several recent studies have demonstrated that the direct precursor of vitamin D3, the calcifediol [25(OH)D3], through the binding to the nuclear vitamin D receptor (VDR), is able to regulate the expression of many genes involved in several cellular processes. Considering that itself may function as a VDR ligand, although with a lower affinity, respect than the active form of vitamin D, we have assumed that 25(OH)D3 by binding the VDR could have a vitamin's D3 activity such as activating non-genomic pathways, and in particular we selected mesenchymal stem cells derived from human adipose tissue (hADMSCs) for the in vitro assessment of the intracellular Ca2+ mobilization in response to 25(OH)D3. Our result reveals the ability of 25(OH)D3 to activate rapid, non-genomic pathways, such as an increase of intracellular Ca2+ levels, similar to what observed with the biologically active form of vitamin D3. hADMSCs loaded with Fluo-4 AM exhibited a rapid and sustained increase in intracellular Ca2+ concentration as a result of exposure to 10-5 M of 25(OH)D3. In this work, we show for the first time the in vitro ability of 25(OH)D3 to induce a rapid increase of intracellular Ca2+ levels in hADMSCs. These findings represent an important step to better understand the non-genomic effects of vitamin D3 and its role in endocrine system.


Assuntos
Calcifediol/farmacocinética , Sinalização do Cálcio/efeitos dos fármacos , Cálcio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Vitaminas/farmacocinética , Humanos , Técnicas In Vitro , Ligantes , Células-Tronco Mesenquimais , Receptores de Calcitriol/metabolismo
18.
J UOEH ; 43(4): 421-426, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34897171

RESUMO

White spongiform nevus is an autosomal dominant inherited disorder first reported by Cannon in 1935. It is a rare disease in which the oral mucosa thickens into an edematous and spongy state and is often accompanied by difficult to recognize subjective symptoms. We report a case of multiple non-hereditary white cavernous nevi in the oral mucosa. The subject was a 22-year-old man with a chief complaint of white lesions in his oral cavity. Examination revealed thick edematous and sponge-like white lesions on the bilateral buccal mucosa, upper and lower lip mucosa, and bilateral lingual margins. There was no history of similar lesions in his family or among his relatives. We diagnosed the case as non-hereditary white sponge nevus, based on clinical and histopathological findings. Although difficult to treat, the lesions disappeared with tetracycline ointment application and oral intake of multiple vitamin supplements. No recurrence of the lesion was observed thereafter.


Assuntos
Mucosa Bucal , Nevo , Adulto , Humanos , Masculino , Pomadas , Tetraciclinas , Vitaminas , Adulto Jovem
19.
Nutrients ; 13(12)2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34960106

RESUMO

Fat-soluble vitamin deficiency remains a challenge in cystic fibrosis (CF), chronic pancreatitis, and biliary atresia. Liposomes and cyclodextrins can enhance their bioavailability, thus this multi-center randomized placebo-controlled trial compared three-month supplementation of fat-soluble vitamins in the form of liposomes or cyclodextrins to medium-chain triglycerides (MCT) in pancreatic-insufficient CF patients. The daily doses were as follows: 2000 IU of retinyl palmitate, 4000 IU of vitamin D3, 200 IU of RRR-α-tocopherol, and 200 µg of vitamin K2 as menaquinone-7, with vitamin E given in soybean oil instead of liposomes. All participants received 4 mg of ß-carotene and 1.07 mg of vitamin K1 to ensure compliance with the guidelines. The primary outcome was the change from the baseline of all-trans-retinol and 25-hydroxyvitamin D3 concentrations and the percentage of undercarboxylated osteocalcin. Out of 75 randomized patients (n = 28 liposomes, n = 22 cyclodextrins, and n = 25 MCT), 67 completed the trial (89%; n = 26 liposomes, n = 18 cyclodextrins, and n = 23 MCT) and had a median age of 22 years (IQR 19-28), body mass index of 20.6 kg/m2 [18.4-22.0], and forced expiratory volume in 1 s of 65% (44-84%). The liposomal formulation of vitamin A was associated with the improved evolution of serum all-trans-retinol compared to the control (median +1.7 ng/mL (IQR -44.3-86.1) vs. -38.8 ng/mL (-71.2-6.8), p = 0.028). Cyclodextrins enhanced the bioavailability of vitamin D3 (+9.0 ng/mL (1.0-17.0) vs. +3.0 ng/mL (-4.0-7.0), p = 0.012) and vitamin E (+4.34 µg/mL (0.33-6.52) vs. -0.34 µg/mL (-1.71-2.15), p = 0.010). Liposomes may augment the bioavailability of vitamin A and cyclodextrins may strengthen the supplementation of vitamins D3 and E relative to MCT in pancreatic-insufficient CF but further studies are required to assess liposomal vitamin E (German Clinical Trial Register number DRKS00014295, funded from EU and Norsa Pharma).


Assuntos
Ciclodextrinas/química , Fibrose Cística/dietoterapia , Lipossomos/química , Triglicerídeos/química , Vitaminas/administração & dosagem , Adolescente , Adulto , Calcifediol/sangue , Colecalciferol/administração & dosagem , Colecalciferol/sangue , Suplementos Nutricionais , Insuficiência Pancreática Exócrina/dietoterapia , Feminino , Humanos , Masculino , Resultado do Tratamento , Vitamina A/administração & dosagem , Vitamina A/sangue , Vitamina D/administração & dosagem , Vitamina D/sangue , Vitamina E/administração & dosagem , Vitamina E/sangue , Vitamina K 2/administração & dosagem , Vitamina K 2/análogos & derivados , Vitaminas/sangue , Vitaminas/química , Adulto Jovem , beta Caroteno/administração & dosagem
20.
Nutrients ; 13(12)2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34960111

RESUMO

Dietary intake, specifically consumption of anti-inflammatory micronutrients, can play a role in both cancer initiation as well as the treatment-related outcomes experienced by patients receiving systemic cancer therapy. Increasing research is being conducted to determine whether micronutrient supplementation can aid in altering the tumor microenvironment (TME), reducing inflammatory side effects and immune-related adverse events (irAEs). However, further research pertaining to the adequacy of dietary micronutrient intake is indicated in the oncology cohort. Currently, no tool measuring dietary intakes of various micronutrients exists in the oncology population. In this study, a 21-item food frequency questionnaire (FFQ) measuring intakes of 14 different micronutrients was validated using diet history as the reference method in 112 oncology patients. Bland Altman plot and Passing Bablok regression analysis were conducted to determine agreement between the two methods. The results showed adequate agreement between FFQ and diet history for 12 nutrients including copper, iron, vitamins A, E, and D, alpha linolenic acid (ALA), long-chain omega 3 fatty acids (LC n3-FA), arginine, glutamic acid, isoleucine, leucine, and valine. This 21-item FFQ, which takes an average of 10 min to complete, can be utilized as a quick screening tool to determine adequacy for 12 different micronutrients in place of a diet history.


Assuntos
Inquéritos sobre Dietas/normas , Dieta/métodos , Micronutrientes/administração & dosagem , Neoplasias/terapia , Idoso , Aminoácidos/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Cobre/administração & dosagem , Registros de Dieta , Ingestão de Alimentos , Ingestão de Energia , Ácidos Graxos Ômega-3/administração & dosagem , Feminino , Humanos , Imunoterapia/métodos , Ferro/administração & dosagem , Masculino , Pessoa de Meia-Idade , Microambiente Tumoral , Vitaminas/administração & dosagem
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