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1.
J Mater Chem B ; 11(32): 7739-7749, 2023 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-37470708

RESUMO

Prolonged and excessive accumulation of ß2-microglobulin (ß2m) in the blood can lead to various kidney-related and other diseases. Currently, the most effective method of removing ß2m from the blood is hemoperfusion. Although some traditional hemoperfusion adsorbents such as cellulose and polystyrene microspheres have been used for the removal of ß2m, their selectivity still needs improvement. Immunosorbents have been developed to address this issue, but high cost and limited application are concerns. TiO2 nanotube arrays (TNTAs) have shown great potential in adsorption-related biomedical applications. In this study, we designed and developed a novel TNTA-based hemoperfusion adsorbent for the removal of ß2m, which has demonstrated good biocompatibility, selectivity, and reusability. We investigated the ß2m adsorption capacities of TNTAs with different pore sizes. The results indicate that TNTAs with a pore size matching the size of ß2m exhibit higher adsorption capacity while also having lower adsorption capacity for albumin, showing the importance of pore size on the selectivity of adsorbents. Additionally, green regeneration of TNTAs is achieved via the photocatalytic activity originating from TiO2. Even after five cycles, the adsorption capacity of TNTAs remained above 70%. Our work demonstrates that inorganic materials with ordered pores are capable to be candidates for hemoperfusion, possessing advantages over traditional organic materials such as high stability, security, and low cost.


Assuntos
Nanotubos , Hemoperfusão/instrumentação , Hemoperfusão/métodos , Nanotubos/química , beta-Globulinas/química , Humanos , Materiais Biocompatíveis/química
2.
Eur Rev Med Pharmacol Sci ; 24(20): 10708-10711, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33155230

RESUMO

OBJECTIVE: The purpose of this study is to bring attention to a case of acute encephalitis not concurrent with acute cerebellitis. CASE PRESENTATION: Five days after onset of common cold symptoms, a 17-months-old girl suffered convulsions, vomiting and respiratory arrest. On exam, she had pharyngeal inflammation, brisk deep tendon reflexes, respiratory acidosis, leukocytosis, negative rapid antigen tests, and segmental pneumonia. Brain CT and MRI/MRA were negative, and EEG was consistent with acute encephalitis. Following hypothermic therapy, methylprednisolone pulse therapy and cefotaxime, she recovered. Four days after discharge, she sustained limb and truncal ataxia associated with normal EEG, followed by bilateral intention tremor. Blood and CSF chemistry and cell counts were normal. Brain MRI revealed high intensity signals in the dentate nuclei and enhancement in the cerebellar white matter, suggestive of acute cerebellitis/cerebellopathy. SPECT imaging showed reduced blood flow in the cerebellum, right thalamus and brain stem. Following short-term administration of g-globulin and prednisolone, she regained her ability to sit and, eventually, to walk. Four months after initial presentation, her brain MRI was normal. No relapse has occurred in 5 years. CONCLUSIONS: The uncommon sequential development of acute encephalitis followed by acute cerebellitis suggests an immune-mediated cerebellar ataxia.


Assuntos
beta-Globulinas/uso terapêutico , Doenças Cerebelares/tratamento farmacológico , Encefalite/tratamento farmacológico , Prednisolona/uso terapêutico , Doença Aguda , beta-Globulinas/administração & dosagem , Doenças Cerebelares/patologia , Encefalite/patologia , Feminino , Humanos , Lactente , Prednisolona/administração & dosagem
3.
Sci Rep ; 10(1): 18904, 2020 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-33144631

RESUMO

Canine babesiosis may cause several hematological and biochemical changes, but only limited studies are available regarding the possible differences of changes in animals infected by different Babesia parasites. The study focused on the evaluation of the differences in serum protein electrophoretic pattern between dogs naturally infected with B. gibsoni (17 dogs) and B. canis (40 dogs). The mean values of total proteins, ß1-, ß2- and γ-globulins were in dogs infected with B. gibsoni significantly higher (P < 0.05 and P < 0.001) than in dogs infected with B. canis. The relative concentrations of albumin, α1-, α2-globulins and the A/G ratios were in the B. gibsoni infected dogs significantly lower (P < 0.001), no significant differences were found in the relative concentrations of ß1- and ß2-globulins. Significant differences were found in most of the evaluated parameters when comparing the results in relation to the form of B. canis infection to B. gibsoni infection. Hematological indices showed significant differences between dogs infected with B. gibsoni and the complicated form of B. canis infection. In conclusion, the obtained results suggest differences in the changes of serum protein electrophoretic pattern between dogs infected with both Babesia species and thus, in the response to the infection caused by various Babesia parasites.


Assuntos
Babesia/classificação , Babesiose/sangue , Eletroforese das Proteínas Sanguíneas/veterinária , Proteínas Sanguíneas/análise , Doenças do Cão/parasitologia , alfa-Globulinas/análise , Animais , beta-Globulinas/análise , Doenças do Cão/sangue , Cães , Feminino , Masculino , Albumina Sérica/análise , gama-Globulinas/análise
4.
Eur J Mass Spectrom (Chichester) ; 26(5): 361-368, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32915641

RESUMO

The monomeric and oligomeric structures of the "FYLLYY" ß2 microglobulin (ß2m) active sequence, formed in (DMSO/CH3CN) solution, were investigated using electrospray ionization (ESI) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Dissociation of dimer and trimer ions was investigated by tandem mass spectrometry using collision induced dissociation (CID). The covalent bond fragmentation patterns were observed in the 21+ and 32+ MS/MS spectra (21+ = [dimer+H]1+ and 32+ = [trimer + 2H]2+). A π-π stacking geometry for the FYLLYY 21+ complex and partial parallel ß-sheet geometry for the 32+ complex are proposed to be stable structures. The observed covalent bond fragment ions in the MS/MS spectra of the 32+ complex are considered to have originated from the partial parallel ß-sheet moiety. The FYLLYY → AALLGY (or FYLLAA) substituted sequence was also investigated by CID-MS/MS. Our MS/MS analysis suggests that the π-π stacking interaction structures are important in dimer binding rather than the structures of a complete parallel or anti-parallel ß-sheet 21+ complex.


Assuntos
Amiloide/química , Peptídeos/química , beta-Globulinas/química , Dimerização , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
5.
Clin Ther ; 42(5): 783-799, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32340917

RESUMO

PURPOSE: Because the results of studies investigating the relation between human papilloma virus (HPV) infection and the effects of psychological stress are inconsistent, this study was conducted to expand on previous research by analyzing patient stress levels, serum immune parameters, and cortisol levels in patients with clinical HPV manifestations. It also looked for differences in clinical manifestations of HPV depending on patient level of experienced stress. METHODS: This cross-sectional study included 213 subjects (94 women and 119 men aged ≥18 years; average age, 41 years) with clinical manifestations of HPV infection (165 subjects with extragenital manifestations and 48 with genital manifestations) who were treated at the Department of Dermatovenerology, Karlovac General Hospital, from January 1, 2012, to December 31, 2015. Psychological, neurohormonal and immune parameters (serum values of leukocytes, alpha2-globulins, beta-globulins, albumins, and proteins), and serum cortisol levels were analyzed. Questionnaires were used to determine patients' perception of stress: the Recent Life Changes Questionnaire, the Perceived Stress Scale, and the Brief Cope Test. One group of subjects had confirmed stressful experiences, defined by the Recent Life Changes Questionnaire as a period of 1 year with at least 500 life change units; the control group included patients with no significant stressful experiences. FINDINGS: Patients with confirmed significant stress experience had a statistically significant higher degree of perception of stress. There were no statistically significant differences in terms of the impact of stress on clinical HPV manifestations (genital and extragenital), sex, lesional duration, or recurrence. In patients with significant stress experience, significantly higher values of leukocytes (6.68 × 109/L), alpha2-globulins (6.85 g/L), and beta-globulins (7.33 g/L) were observed. Adaptive coping and a lower perception of stress significantly reduced the chances of having extragenital manifestations by 2.63 times. A higher perception of stress significantly increased the likelihood of genital manifestations. IMPLICATIONS: Although this study found that stress increased the values of leukocytes, alpha2-globulins, and beta-globulins, no evidence was found that it affected clinical manifestations of HPV infection. The redundancy of the immune system could account for this finding. This study is among the first to investigate the correlation between psychological, neurohormonal, and immune indicators of stress.


Assuntos
Hidrocortisona/sangue , Infecções por Papillomavirus/sangue , Infecções por Papillomavirus/imunologia , Estresse Psicológico/sangue , Estresse Psicológico/imunologia , Adaptação Psicológica , Adulto , Idoso , alfa-Globulinas/análise , beta-Globulinas/análise , Estudos Transversais , Feminino , Humanos , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Adulto Jovem
7.
Toxicon ; 134: 18-25, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28528178

RESUMO

Tityus serrulatus (Ts) venom is composed of a mixture of toxins presenting diverse biological functions. However, although this venom has been studied over the past three decades, omics analysis revealed that most of its toxins are not identified or their biological activities are unknown. Ts19 Frag-II is included in this group, which function is still uncertain. This study aimed to expand the biological activities of Ts19 Frag-II through in vivo investigation. Our results demonstrates that mice challenged with Ts19 Frag-II presented biochemical alterations, increasing serum levels of urea, ALT and ß-globulin, besides decreasing γ-globulins. Moreover, this toxin was also able to induce immunological alterations, increasing NO, IL-6, TNF-α and IL-17, being considered a proinflammatory toxin. The increase of IL-17 was unprecedented regarding Ts toxins and could be a result of the overall produced-effect of cells of innate immunity cells (neutrophils, monocytes, natural killers and lymphoid tissue inducers - LTis) as well as of adaptive immunity (Th17 cells). This study expanded the biological activities of Ts19 Frag-II, suggesting that this toxin could be contributing to the Ts envenoming through alterations of biochemical parameters as well as triggering the inflammatory response.


Assuntos
Interleucina-17/sangue , Peptídeos/imunologia , Peptídeos/toxicidade , Venenos de Escorpião/imunologia , Venenos de Escorpião/toxicidade , Alanina Transaminase/sangue , Animais , beta-Globulinas/análise , Imunidade Inata , Interleucina-6/sangue , Masculino , Camundongos Endogâmicos BALB C , Óxido Nítrico/sangue , Escorpiões/química , Fator de Necrose Tumoral alfa/sangue , Ureia/sangue , gama-Globulinas/análise
8.
Nuklearmedizin ; 56(6): 239-242, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29533419

RESUMO

AIM: The liver is an important reference organ for positron emission tomography/ computed tomography (PET-CT) examination using 18F-fluorodeoxyglucose (18F-FDG). However, 18F-FDG uptake by the liver is affected by many factors. We therefore investigated the effect of hepatic function on 18F-FDG uptake in the liver. METHODS: A retrospective analysis of data on the hepatic function and the mean liver standardized up-take value (SUV) of 18F-FDG uptake in the liver during PET-CT examination of 500 (381 males, 119 females, aged 27-71) physical examinees. RESULTS: The mean liver SUV was 1.88 ± 0.20. The correlation coefficient and partial correlation coefficient for age, the levels of conjugated bilirubin, globulin, AST and the mean liver SUV were statistically significant (r' = 0.119, -0.197, -0.089 and 0.151, all p < 0.05). Multiple linear regression analysis showed that age and the levels of conjugated bilirubin, globulin and aspartate amino-transferase (AST) were independent factors that influenced changes in the mean liver SUV (ß = 0.008, -0.025, -0.151 and 0.005, all p < 0.05). The globulin level had the biggest predictive ability (ß' = -0.151, p < 0.05). CONCLUSIONS: The uptake of 18F-FDG in the liver was influenced by some liver function indexes. The levels of conjugated bilirubin, globulin and AST were independent factors for predicting changes in the uptake of 18F-FDG in the liver. Liver function test results should be combined with an evaluation of the metabolic activity of the liver.


Assuntos
Fluordesoxiglucose F18 , Fígado/diagnóstico por imagem , Fígado/metabolismo , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Compostos Radiofarmacêuticos , Adulto , Idoso , Aspartato Aminotransferases/metabolismo , beta-Globulinas/metabolismo , Bilirrubina/metabolismo , Transtornos da Coagulação Sanguínea/congênito , Transtornos da Coagulação Sanguínea/metabolismo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
9.
Food Chem ; 221: 1874-1882, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-27979176

RESUMO

Many biochemical events occur inside grains during post-harvest processes. Several methods have been developed to relate the chemical composition of the coffee grain to the beverage quality, including identification of possible molecular markers for flavor characterizing. This study was aimed at evaluating the changes in the proteomic profile of pulped and natural C. arabica grains dried in a yard or dryer at 60°C. It was observed that fruits dried in a dryer at 60°C showed an altered proteomic profile, with a reduction in the most abundant proteins compared to those yard-dried grains. Among the identified proteins, those involved in the metabolism of sugars and stress response were highlighted. Results have shown that post-harvest processes that impact coffee quality are related to changes in protein abundance, indicating that proteomic analysis may be effective in the identification of biochemical changes in coffee grains subjected to different post-harvest processes.


Assuntos
Coffea/química , Café/química , Dessecação , Manipulação de Alimentos , Proteômica , beta-Globulinas/análise , Gliceraldeído-3-Fosfato Desidrogenases/análise , Proteínas de Choque Térmico/análise , Proteínas de Plantas/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem , UTP-Glucose-1-Fosfato Uridililtransferase/análise , alfa-Galactosidase/análise
10.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-23106

RESUMO

In Korea, recent epidemiologic studies show that the incidence of β-thalassemia is increasing as the influx of South-East Asian population increases and molecular technologies develop. However, many patients are still misdiagnosed as iron deficiency anemia (IDA). All patients with microcytic anemia need to perform evaluation including reticulocyte index, Mentzer index, and iron studies. Considering the increasing incidence of β-thalassemia, hemoglobin beta globulin (HBB) gene sequencing should be performed if suspicious. In our cases, patients whose parents were both Koreans were confirmed to have β-thalassemia with a substitution in c1, ATG>GTG, and deletion of the HBB gene. In Korea, initiation condon ATG>AGG (20.9%) is most common mutation, followed by codon 17 (A>T) (17.6%), codon 121 (G>T) (12.1%), and so on. We report two cases of β-thalassemia diagnosed by genetic testing for microcytic anemia.


Assuntos
Humanos , Anemia , Anemia Ferropriva , Povo Asiático , beta-Globulinas , Talassemia beta , Códon , Estudos Epidemiológicos , Testes Genéticos , Incidência , Ferro , Coreia (Geográfico) , Pais , Reticulócitos
11.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-788608

RESUMO

In Korea, recent epidemiologic studies show that the incidence of β-thalassemia is increasing as the influx of South-East Asian population increases and molecular technologies develop. However, many patients are still misdiagnosed as iron deficiency anemia (IDA). All patients with microcytic anemia need to perform evaluation including reticulocyte index, Mentzer index, and iron studies. Considering the increasing incidence of β-thalassemia, hemoglobin beta globulin (HBB) gene sequencing should be performed if suspicious. In our cases, patients whose parents were both Koreans were confirmed to have β-thalassemia with a substitution in c1, ATG>GTG, and deletion of the HBB gene. In Korea, initiation condon ATG>AGG (20.9%) is most common mutation, followed by codon 17 (A>T) (17.6%), codon 121 (G>T) (12.1%), and so on. We report two cases of β-thalassemia diagnosed by genetic testing for microcytic anemia.


Assuntos
Humanos , Anemia , Anemia Ferropriva , Povo Asiático , beta-Globulinas , Talassemia beta , Códon , Estudos Epidemiológicos , Testes Genéticos , Incidência , Ferro , Coreia (Geográfico) , Pais , Reticulócitos
12.
Crit Rev Oncol Hematol ; 107: 182-189, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27823646

RESUMO

Non-Hodgkin lymphoma (NHL) is one of the most common malignancies and a major cause of morbidity and mortality. Radioimmunotherapy (RIT) is a novel modality for treating NHL which offers the combined use of monoclonal antibodies for specific targeting of malignant cells and radiation for killing these cells. Despite the promising results favoring RIT in several clinical studies in different target populations and NHL types, Food and Drug Administration (FDA) approval for RIT agents is restricted to a limited number of indications and agents, maybe because of several ambiguities that still exist in the field. One of these ambiguities are the lack of evidence-based prognostic factors that determine what patient population would benefit most from RIT, which is essential to know in order to optimize the efficacy and safety of treatment with RIT. As well as selecting the best patient population for RIT, it is important to assess the response to RIT in order to provide further treatment strategies or avoid unnecessary therapies and diagnostic procedures. In this review we have explored the details of how to predict the efficiency of RIT based on various prognostic factors that have been investigated in the evidence, and also discussed the proposed methods and timing schedules for assessing the response to RIT. We have also pointed out the ambiguities in the aforementioned topics, which call for more investigation.


Assuntos
Linfoma não Hodgkin/terapia , Radioimunoterapia , Fatores Etários , beta-Globulinas/análise , Humanos , L-Lactato Desidrogenase/sangue , Linfoma não Hodgkin/imunologia , Linfoma não Hodgkin/radioterapia
13.
Clin Chim Acta ; 460: 220-6, 2016 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-27417394

RESUMO

BACKGROUND: Given the excellent performance of modern mass spectrometers, their clinical application for the analysis of macromolecules is a growing field of interest. This principle is explored by hemoglobin analysis, which is a representative example by its molecular weight and clinical relevance in e.g. screening programs for thalassemia and hemoglobin variants. Considering its abundance and cellular containment, pre-analysis is significantly reduced allowing for essential rapid acquisitions. METHODS: By parallel analysis of routine diagnostics for hemoglobin variants and thalassemia, we acquired samples of adults who were consented for hemoglobinopathy screening in our clinical laboratory. The pre-analytical process comprised of red cell lysis only; without further digestion and purification steps, the samples were directly injected in an electrospray ionization quadrupole time-of-flight setup and the intact proteins were analyzed by flow injection analysis. After optimization of process parameters, the deconvoluted mass spectra revealed the presence of α- and ß-globulins. The reference ranges for the average mass of both globulins and their intensity ratio (α/ß-ratio) were deduced from a disease-free subgroup and patients with a hemoglobinopathy were compared. RESULTS: The α/ß-ratio is a poor marker for thalassemia patients, yet deviant α/ß-ratios are found for patients with a hemoglobin variant. Mass deviations down to 1Da can be resolved; even if the patient suffers from a heterozygotic disorder, the average mass is found outside the established reference interval. CONCLUSIONS: Although subjects with mild thalassemia were not detected, all patients with a hemoglobin variant were resolved by top-down mass spectrometry using the average globulin mass and the α/ß-ratio as screening parameters.


Assuntos
Hemoglobinopatias/diagnóstico , Hemoglobinas Anormais/análise , Espectrometria de Massas/métodos , Adulto , alfa-Globulinas/análise , beta-Globulinas/análise , Humanos , Programas de Rastreamento , Valores de Referência , Espectrometria de Massas por Ionização por Electrospray/métodos , Fatores de Tempo
14.
Childs Nerv Syst ; 32(8): 1431-40, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27325303

RESUMO

AIM: The aim of the study was to evaluate renal function and to assess the usefulness of the following nephrotoxicity markers: cystatin C (CYS C), beta-2 microglobulin (B2MG) and neutrophil gelatinase-associated lipocalin (NGAL) in 38 (18 girls, 20 boys) children previously treated for central nervous system malignancy. MATERIAL: Median age at evaluation was 13.7 years (range 2.1-22 years). The mean follow-up time after the completion of chemotherapy was 3.2 years (range 0.16-6.5 years). RESULTS: Subclinical chronic kidney disease (estimated glomerular filtration rate: eGFR 90-60 ml/min/1.73 m(2)) was found in 22 patients (58 %), while renal insufficiency (eGFR 30-60 ml/min/1.73 m(2)) was found in six children (16 %). It has been demonstrated statistically significant negative correlation between the eGFR and cystatin C concentration (p < 0.0001) and eGFR and beta-2 microglobulin concentration (p < 0.02). Conversely, there was no correlation between eGFR and NGAL. Thirteen children (34 %) developed drug-induced tubulopathy: decreased tubular reabsorption of phosphate (TRP) and renal tubular threshold for phosphate (Tmp/GFR). CONCLUSION: Children treated for CNS tumours often develop drug-induced chronic renal disease, involving the glomeruli and/or renal tubules. Cystatin C and beta-2 microglobulin seemed to be good markers for chronic kidney damage in these patients, which is probably not true for NGAL.


Assuntos
Antineoplásicos/efeitos adversos , Nefropatias/induzido quimicamente , Nefropatias/diagnóstico , Adolescente , beta-Globulinas/metabolismo , Neoplasias do Sistema Nervoso Central/tratamento farmacológico , Criança , Pré-Escolar , Cistatina C/metabolismo , Feminino , Seguimentos , Taxa de Filtração Glomerular , Humanos , Nefropatias/metabolismo , Lipocalina-2/metabolismo , Masculino , Estatísticas não Paramétricas , Adulto Jovem
15.
Artigo em Chinês | MEDLINE | ID: mdl-26596012

RESUMO

OBJECTIVE: To observe the characteristics of serum protein in patients with benign paroxysmal positional vertigo (BPPV) during the symptomatic period. METHOD: Fifty-five patients with BPPV and 58 control subjects were enrolled in the study. All the patients underwent the Dixe-Hallpike and Roll maneuver to confirm the type of BPPV. The average time of onset was (1.0 ± 0.8)days in the group of BPPV. The clinical features and the laboratory tests of serum protein electrophoresis, blood counts, and liver and kidney function were performed in both groups. RESULT: The levels of serum albumin (Alb), α1 globulin, α2 globulin and ß globulin of BPPV group did not differ statistically in the two groups (P > 0.05); The γ-globulin levels were significantly higher in patients with BPPV than in controls (P < 0.05). CONCLUSION: The γ-globulin levels are increased when BPPV is attacking.


Assuntos
Vertigem Posicional Paroxística Benigna/sangue , Vertigem Posicional Paroxística Benigna/diagnóstico , gama-Globulinas/análise , alfa-Globulinas/análise , beta-Globulinas/análise , Estudos de Casos e Controles , Humanos , Albumina Sérica/análise
18.
Toxicon ; 95: 67-71, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25576236

RESUMO

Chemical analyses of the hemagglutinating fraction from Scorpaena plumieri venom revealed that it contains five components (Sp-CL 1-5) with similar chromatographic elution profiles (35-38% of acetonitrile), molecular masses (16,800-17,000 Da) and N-terminal sequences, suggesting that they are isoforms of the same protein. The amino acid sequence of Sp-CL4 was determined and shown to have homology with fish C-type lectins. These data demonstrate for the first time the presence of C-type isolectins in a scorpionfish venom.


Assuntos
Venenos de Peixe/química , Lectinas/isolamento & purificação , Perciformes , Sequência de Aminoácidos , Animais , beta-Globulinas/química , beta-Globulinas/isolamento & purificação , Venenos de Peixe/isolamento & purificação , Lectinas/química , Lectinas Tipo C/química , Lectinas Tipo C/isolamento & purificação , Dados de Sequência Molecular , Peso Molecular , Alinhamento de Sequência
19.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-747876

RESUMO

OBJECTIVE@#To observe the characteristics of serum protein in patients with benign paroxysmal positional vertigo (BPPV) during the symptomatic period.@*METHOD@#Fifty-five patients with BPPV and 58 control subjects were enrolled in the study. All the patients underwent the Dixe-Hallpike and Roll maneuver to confirm the type of BPPV. The average time of onset was (1.0 ± 0.8)days in the group of BPPV. The clinical features and the laboratory tests of serum protein electrophoresis, blood counts, and liver and kidney function were performed in both groups.@*RESULT@#The levels of serum albumin (Alb), α1 globulin, α2 globulin and β globulin of BPPV group did not differ statistically in the two groups (P > 0.05); The γ-globulin levels were significantly higher in patients with BPPV than in controls (P < 0.05).@*CONCLUSION@#The γ-globulin levels are increased when BPPV is attacking.


Assuntos
Humanos , alfa-Globulinas , Vertigem Posicional Paroxística Benigna , Sangue , Diagnóstico , beta-Globulinas , Estudos de Casos e Controles , Albumina Sérica , gama-Globulinas
20.
Indian J Pathol Microbiol ; 57(4): 626-8, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25308024

RESUMO

In India, hemoglobinopathies constitute a major genetic disorder and hemoglobin variants such as Hb S, Hb D Punjab, and Hb E are the most common ones. Other variants include Hb Q India, Hb Lepore, Hb J Meerut, Hb D Iran, etc. These variants show heterozygous state along with beta thalassemia. However, compound heterozygosities among these variants are very rare. Ethylenediaminetetraacetic acid whole blood sample received for routine thalassemia screening was subjected to alkaline electrophoresis using automated capillary zone electrophoresis. Suspecting the presence of rare variants, further analysis was carried out using Bio-Rad D10 and Tosoh G8 high-performance liquid chromatography (HPLC) systems. Capillary zone electrophoretograms showed the presence of peaks in zone Hb A, Hb D, a fused peak in Hb A2, and a small peak in Z1 zone. Bio-Rad and Tosoh chromatograms also indicated the presence of four peaks which are identified as Hb A, Hb D Punjab, Hb Q India, and hybrid of Hb D Punjab/Hb Q India. A peak in Hb D zone of capillary was due to co-migration of Hb D Punjab and Hb Q India variants. Small peak in Z1 zone indicated the presence of alpha chain variant Hb Q India. The findings were further confirmed by HPLC results and molecular genetic studies. The present study reports for the 1st time a rare hemoglobinopathy of double heterozygosity for Hb D Punjab, Hb Q India on Capillarys 2 Flex Piercing analyzer and is forth reported case for this rare hemoglobinopathy.


Assuntos
Hemoglobinopatias/diagnóstico , Hemoglobinopatias/genética , Hemoglobinas Anormais/genética , Substituição de Aminoácidos , beta-Globulinas/genética , Eletroforese Capilar , Humanos , Índia , Reação em Cadeia da Polimerase , alfa-Globinas/genética
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