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2.
Methods Mol Biol ; 2246: 301-316, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33576998

RESUMO

This overview addresses fluorescence in situ hybridization (FISH) in a diagnostic microbiology setting with its associated problems and pitfalls and how to control them, but also the advantages and opportunities the method offers. This article focuses mainly on diagnostic FISH assays on tissue sections and on techniques and experiences in our laboratory. FISH in a routine diagnostic setting in microbiology requires strict quality control measures to ensure consistent high-quality and reliable assay results. Here, for the first time, we define quality control requirements for microbiological diagnostic FISH applications and discuss their impact and possible future developments of the FISH technique for infection diagnostics. We focus on diagnosis of biofilm-associated infections including infective endocarditis, oral biofilms, and device-associated infections as well as infections due to fastidious or yet uncultured microorganisms like Treponema spp., Tropheryma whipplei, Bartonella, Coxiella burnetii, or Brachyspira.


Assuntos
Bactérias/genética , Hibridização in Situ Fluorescente/métodos , Biofilmes/crescimento & desenvolvimento , Endocardite Bacteriana/microbiologia , Fluorescência , Microbiologia , Controle de Qualidade
5.
Microbiology (Reading) ; 167(3)2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33529149

RESUMO

The sixth Young Microbiologists Symposium on 'Microbe Signalling, Organisation and Pathogenesis' was scheduled to be held at the University of Southampton, UK, in late August 2020. However, due to the health and safety guidelines and travel restrictions as a response to the COVID-19 pandemic, the symposium was transitioned to a virtual format, a change embraced enthusiastically as the meeting attracted over 200 microbiologists from 40 countries. The event allowed junior scientists to present their work to a broad audience and was supported by the European Molecular Biology Organization, the Federation of European Microbiological Societies, the Society of Applied Microbiology, the Biochemical Society, the Microbiology Society and the National Biofilms Innovation Centre. Sessions covered recent advances in all areas of microbiology including: Secretion and transport across membranes, Gene regulation and signalling, Host-microbe interactions, and Microbial communities and biofilm formation. This report focuses on several of the highlights and exciting developments communicated during the talks and poster presentations.


Assuntos
Interações Hospedeiro-Patógeno , Interações Microbianas , Microbiologia/tendências , Biofilmes , Congressos como Assunto , Humanos , Reino Unido , Comunicação por Videoconferência
6.
J Leukoc Biol ; 109(3): 513-517, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33630385

RESUMO

Metchnikoff's essay, Intestinal Bacteriotherapy, was written when the study of microbiology was still in its infancy and few intestinal diseases had been ascribed to a specific bacterial infection. Metchnikoff offered perceptive ideas that have become standard in today's science. This Historical Perspectives commentary examines how Metchnikoff's article influenced our field. An accompanying editorial by Siamon Gordon explores this topic further and describes the relevance of Metchnikoff's work to the current Covid-19 infection. We also include a translation of this fundamental article by Metchnikoff, as presented by Claudine Neyen.


Assuntos
Microbioma Gastrointestinal/fisiologia , Microbiologia/história , Animais , História do Século XIX , História do Século XX , Humanos
7.
J Med Microbiol ; 70(3)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33404382

RESUMO

2020 was the year when microbiology burst onto the world stage, not just as the science of small living things, but as the prism through which we understood global events. Clinical logic suffered under pressure arising from an urgent need to confirm or exclude severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. This is a generation's Hobbesian moment in which the public concern for safety and security from infection outweighs the pursuit of personal freedom. The strangeness of a world in which a minute particle wields superhuman power has generated its list of unlikely heroes and mendacious villains. As the year comes to an end, there are glimmers of light amid the gloom: the prospect of an effective vaccine, and life after the pandemic.


Assuntos
/epidemiologia , /prevenção & controle , /diagnóstico , Humanos , Microbiologia , Filosofia , Saúde Pública
9.
Diagn Microbiol Infect Dis ; 100(1): 115309, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33486387

RESUMO

Diagnostic microbiology services form a critical component of the response to infectious disease outbreaks. Like previous respiratory virus pandemics, the COVID-19 pandemic has placed significant strains on the standing capacity of laboratories around the world. In this case study, we describe the surge response required by our laboratory to meet the fluctuating demand for SARS-CoV-2 in our regional pathology service in Western Sydney, Australia between March and May 2020. While the overall number of SARS-CoV-2 PCR positive cases was relatively low compared to other Australian local health districts, testing numbers were highly unpredictable and changed on a weekly basis as local outbreaks were detected. As with other laboratories, numerous other challenges were also faced during this period, including the requirement to introduce a new and unaccredited diagnostic PCR assay for SARS-CoV-2, local and global shortages of reagents for sampling and sample processing, and a significant institutional SARS-CoV-2 outbreak in our laboratory catchment area. A successful service delivery during this period could only be maintained by a dynamic whole-of-laboratory and organizational response including (1) operational changes to the hours of service and the expansion of diagnostic testing at our laboratory site and other sites within our organization (2) careful management of specialist staff and re-training and recruitment of additional staff (3) changes to laboratory workflows to improve SARS-CoV-2 PCR test turnaround time and to accommodate limits to precious laboratory reagents; (4) clear communication within our laboratory and the NSW Health Pathology organization; and (5) collaborative co-ordination and support by NSW Health Pathology.


Assuntos
Laboratórios/organização & administração , Microbiologia , Austrália , Serviços de Laboratório Clínico/organização & administração , Serviços de Laboratório Clínico/estatística & dados numéricos , Humanos , Laboratórios/provisão & distribução , Pessoal de Laboratório Médico/educação , Reação em Cadeia da Polimerase , Fatores de Tempo
10.
Nat Microbiol ; 6(1): 1-2, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33349680
12.
Rev. cuba. inform. méd ; 12(2): e379, tab, graf
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1144461

RESUMO

Introducción: como parte del proceso de formación de enfermeros, médicos y tecnólogos de la salud son habilitados temas relacionados con la microbiología. Sin embargo, a partir del conjunto de medidas de seguridad y la disponibilidad de recursos físicos, no es posible el estudio de diversos microorganismos. Objetivo: desarrollar un Sistema de Laboratorios Remoto para la práctica de Microbiología y Parasitología Médica. Materiales y métodos: el sistema de Laboratorios Remoto posee un microscopio electrónico controlado mediante una interface de comunicación con un ordenador conectado a la red. Resultados: se obtuvo como resultado un Sistema de Laboratorios Remoto que puede ser accedido mediante Internet o la red institucional. Facilita el estudio y la interpretación de diferentes muestras biológicas. Brinda un conjunto de reportes y estadísticas que permiten realizar análisis históricos de comportamiento. Conclusiones: a partir del desarrollo de las prácticas de laboratorios a distancia, es posible el estudio de diferentes microorganismos sin riesgos biológicos para el estudiante(AU)


Introduction: as part of the training process for nurses, physicians and health technologists, topics related to microbiology are enabled. However, based on the set of security measures and the availability of physical resources, the study of various microorganisms is not possible. Objective: to develop a Remote Laboratory System for the practice of the subject Medical Microbiology and Parasitology. Methods: the Remote Laboratory System has an electronic microscope controlled by a communication interface with a computer connected to the network. Results: a Remote Laboratory System that can be accessed through the Internet or the institutional network. The system facilitates the study and interpretation of different biological samples and also provides a set of reports and statistics that allow for historical behavior analysis. Conclusions: from the development of remote laboratory practices, it is possible to study different microorganisms without biological risks for the student(AU)


Assuntos
Software , Sistemas de Informação em Laboratório Clínico , Telemedicina , Microbiologia
14.
mSphere ; 5(5)2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-32999084

RESUMO

The inaugural Black In Microbiology Week (#BlackInMicro) is 28 September 2020 through 4 October 2020. Its mission is to "showcase the presence and accomplishments of Black microbiologists from around the globe, connect Black microbiologists with one another and foster a sense of community among them, and provide a forum for the discussion of racial disparities in microbiology and its subfields." Participation in this event will happen primarily over Twitter through the hashtag #BlackInMicro and over Zoom through registration on the website https://blackinmicrobiology.org/ An additional mission of Black In Microbiology Week is to amplify black scientists. Today, mSphere does this by presenting two mSphere of Influence commentaries from Black In Microbiology co-lead organizers Ariangela J. Kozik ("mSphere of Influence: frameshift-a vision for human microbiome research" [mSphere 5:e00944-20, 2020, https://doi.org/10.1128/mSphere.00944-20]) and Kishana Taylor ("mSphere of Influence: that's racist-COVID-19, biological determinism, and the limits of hypotheses" [mSphere 5:e00945-20, 2020, https://doi.org/10.1128/mSphere.00945-20]).


Assuntos
Grupo com Ancestrais do Continente Africano/genética , Determinismo Genético , Racismo/prevenção & controle , Betacoronavirus , Infecções por Coronavirus/epidemiologia , Humanos , Microbiologia , Microbiota , Pandemias , Pneumonia Viral/epidemiologia
16.
Int J Syst Evol Microbiol ; 70(10): 5594-5595, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32956033

RESUMO

The Senate of The University of Queensland, on the recommendation of the Executive Board of the International Committee on Systematics of Prokaryotes, is pleased to present the van Niel International Prize for Studies in Bacterial Systematics for the triennium 2017-2020 to Dr Tanja Woyke in recognition of her contributions made to the field of bacterial systematics. The award, established in 1986 by Professor V. B. D. Skerman of The University of Queensland, honours the contribution of scholarship in the field of microbiology by Professor Cornelis Bernardus van Niel.


Assuntos
Distinções e Prêmios , Bactérias/classificação , Microbiologia/história , História do Século XXI , Queensland , Terminologia como Assunto
17.
Sanid. mil ; 76(3): 197-204, jul.-sept. 2020. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-198586

RESUMO

Telemedicine consists of the exchange of health information between healthcare professionals, and between healthcare professionals and patients, through the use of information and communications technologies (ICT). It is already considered an emerging technology in what is commonly called the «Productivity Plateau». It is also probably an exponential growth technology, in which the «trigger» for such growth will be a mixture of new technologies such as portable sensors/ wearables that detect multiple patient data (Blood Pressure, Heart Rate, Respiratory Rate, Glycaemia, Temperature, etc.), better communications (5G), augmented and mixed reality (augmented and virtual), artificial intelligence systems to improve diagnosis, etc. In Spain, Military Telemedicine is a pioneer in the field. The main mission is to provide remote health support to health professionals or military personnel deployed in Operations and remote or difficult-to-access locations. In 2021 the Spanish Telemedicine Unit at Central Defense Hospital «Gómez Ulla» will celebrate its 25Th anniversary. This article discusses the aforementioned aspects of telemedicine as an emerging technology and describes the current mission, organization and capabilities of Spanish military telemedicine, as well as its future


La Telemedicina consiste en el intercambio de información sanitaria entre profesionales sanitarios, o entre profesional sanitario y paciente, mediante el uso de las tecnologías de la información y comunicaciones (TIC). Se considera ya una tecnología emergente en el denominado «Plateau de productividad». Probablemente se trate de una tecnología de crecimiento exponencial, en la cual el «gatillo» para dicho crecimiento será una mezcla de diferentes tecnologías, como nuevos sensores portátiles que detecten múltiples datos de los pacientes (Tensión Arterial, Frecuencia cardiaca, Frecuencia respiratoria, Glucemia, Temperatura, etc), mejores comunicaciones (5G), realidad aumentada y mixta (aumentada y virtual), sistemas de inteligencia artificial para ayuda al diagnóstico, etc. En España, la Telemedicina Militar es pionera en este campo. Su misión fundamental es el apoyo y asesoramiento sanitario a distancia a personal sanitario y también no sanitario, tanto en Zona de Operaciones (ZO) como en situación de aislamiento y/o localización remota. En 2021 el Servicio de Telemedicina del Hospital Central de la Defensa «Gomez Ulla» celebrará su 25 aniversario. En el artículo se tratan los mencionados aspectos acerca de la Telemedicina como tecnología emergente y se describe la misión, organización y capacidades actuales de la Telemedicina Militar Española, así como sus perspectivas de futuro


Assuntos
Humanos , História do Século XXI , Telemedicina/história , Telemedicina/tendências , Medicina Militar/história , Medicina Militar/tendências , Telemedicina/métodos , Evacuação/normas , Microbiologia/tendências
18.
Nat Commun ; 11(1): 4097, 2020 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-32796861

RESUMO

Staphylococcus aureus is generally thought to divide in three alternating orthogonal planes over three consecutive division cycles. Although this mode of division was proposed over four decades ago, the molecular mechanism that ensures this geometry of division has remained elusive. Here we show, for three different strains, that S. aureus cells do not regularly divide in three alternating perpendicular planes as previously thought. Imaging of the divisome shows that a plane of division is always perpendicular to the previous one, avoiding bisection of the nucleoid, which segregates along an axis parallel to the closing septum. However, one out of the multiple planes perpendicular to the septum which divide the cell in two identical halves can be used in daughter cells, irrespective of its orientation in relation to the penultimate division plane. Therefore, division in three orthogonal planes is not the rule in S. aureus.


Assuntos
Proteínas de Bactérias/metabolismo , Staphylococcus aureus/citologia , Staphylococcus aureus/metabolismo , Proteínas de Bactérias/genética , Microbiologia , Imagem com Lapso de Tempo
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