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1.
Anal Bioanal Chem ; 411(7): 1301-1309, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30793214

RESUMEN

NMR and LC-MS are two powerful techniques for metabolomics studies. In NMR spectra and LC-MS data collected on a series of metabolite mixtures, signals of the same individual metabolite are quantitatively correlated, based on the fact that NMR and LC-MS signals are derived from the same metabolite covary. Deconvoluting NMR spectra and LC-MS data of the mixtures through this kind of statistical correlation, NMR and LC-MS spectra of individual metabolites can be obtained as if the specific metabolite is virtually isolated from the mixture. Integrating NMR and LC-MS spectra, more abundant and orthogonal information on the same compound can significantly facilitate the identification of individual metabolites in the mixture. This strategy was demonstrated by deconvoluting 1D 13C, DEPT, HSQC, TOCSY, and LC-MS spectra acquired on 10 mixtures consisting of 6 typical metabolites with varying concentration. Based on statistical correlation analysis, NMR and LC-MS signals of individual metabolites in the mixtures can be extracted as if their spectra are acquired on the purified metabolite, which notably facilitates structure identification. Statistically correlating NMR spectra and LC-MS data (CoNaM) may represent a novel approach to identification of individual compounds in a mixture. The success of this strategy on the synthetic metabolite mixtures encourages application of the proposed strategy of CoNaM to biological samples (such as serum and cell extracts) in metabolomics studies to facilitate identification of potential biomarkers.


Asunto(s)
Cromatografía Liquida/métodos , Espectroscopía de Resonancia Magnética/métodos , Metabolómica/métodos , Espectrometría de Masas en Tándem/métodos , Metaboloma , Flujo de Trabajo
5.
Brasília; Ministério da Saúde; 1986. 11 p.
Monografía en Portugués | LILACS, Coleciona SUS, Repositorio RHS, CNS-BR | ID: lil-786903

RESUMEN

O evento, que contou com mais de 1.000 participantes, foi a culminação de todo um processo de discussão iniciado na maioria dos estados brasileiros, através da realização de Pré-Conferências que receberam significativa presença de diversos segmentos da população e da categoria odontológica. Do total dos participantes, 286 eram delegados oficiais com direito a voz e voto, representando a Odontologia propriamente dita (Conselhos Regionais, ABOs e Sindicatos), Secretarias Estaduais e Municipais de Saúde, INAMPS, ABENO, Fundação SESP, Centrais de Trabalhadores (CGT e CUT), Associações de Moradores (CONAM) e Estudantes. Na história da Odontologia Brasileira, não há registro de momento semelhante, sob o ponto de vista democrático, onde a problemática de saúde bucal da população tenha sido exposta e discutida de forma tão pluralista. Desta vez, não se tratava simplesmente de um encontro exclusivo de Cirurgiões-Dentistas.


Asunto(s)
Sistemas de Salud , Salud Bucal , Fuerza Laboral en Salud , Política de Salud , Recursos en Salud , Brasil
6.
Eur Biophys J ; 13(1): 1-9, 1985.
Artículo en Inglés | MEDLINE | ID: mdl-2416556

RESUMEN

An alkylated monomer of Concanavalin A was prepared photochemically according to the method of Tanaka et al. (1981). Its affinities for methyl-alpha-D-gluco, methyl-alpha-D-manno and p-nitrophenyl-alpha-D-manno pyranoside were calculated. The enthalpies of these binding reactions were measured calorimetrically and the thermodynamic parameters were calculated. The values obtained suggest that the structure of the monomer differs from that of the dimeric and tetrameric molecules. Calorimetric studies also showed that the monomeric derivative reacts with IgM but not IgG. The enthalpy per binding site in the monomer-IgM reaction is equal to that of the monomer-mannose derivative reaction; mannose is the terminal residue of the saccharide chains of the IgM molecule. The stoichiometry of the reaction is ten ConA-m per IgM molecule.


Asunto(s)
Concanavalina A , Aminoácidos/análisis , Calorimetría , Carbohidratos , Fenómenos Químicos , Química , Humanos , Inmunoglobulina G , Inmunoglobulina M , Cinética , Termodinámica , Macroglobulinemia de Waldenström/inmunología , gammaglobulinas
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