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1.
Enferm Clin ; 31: S78-S83, 2021 Feb.
Artículo en Español | MEDLINE | ID: mdl-34629854

RESUMEN

Aim: Personal protective equipment (PPE), including respirators, is essential in a pandemic like COVID-19, which has required, on many occasions, the reuse of material due to its shortage. The aim of this review is to summarize available evidence on the reuse and extended use of filtering facepiece respirator. Method: Scoping review. Search through natural language in PUBMED and Centers, Agencies and Organizations for Disease Control. Limited to articles published between 2010-2020 in English and Spanish. Results: 83 articles were located, 14 were selected, plus 5 recommendations. The topics included in this study are classified in 7 sections: expiration, extended use and reuse of masks, handling techniques, sealing, physical-psychological effects and compliance, contamination and decontamination of respirators. Conclusions: The reuse of masks is not recommended by official organizations or manufacturers, and is only accepted in extraordinary cases, such as pandemics. The studies are characterized by having small samples, using different models of respirators adjusting their recommendation to the model.


Asunto(s)
COVID-19 , Equipo Reutilizado , Descontaminación , Humanos , SARS-CoV-2 , Ventiladores Mecánicos
2.
Waste Manag Res ; 39(11): 1365-1374, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34617479

RESUMEN

This article presents field experience in developing local waste management plans called Kebijakan Strategi Daerah (Jakstrada) in the Lake Toba region, one of the popular tourist destinations in Indonesia. It examines the current waste management status and discusses key priorities and actions identified in the Jakstradas to improve the waste management based on resource management and 3R (reduce, reuse and recycle) principles. In partnership with public, private, academic and citizen groups, the project activities supported the development of Jakstradas in all seven regencies and established a resource recovery centre (RRC) as a model to practice 3Rs in the region. The key lessons learned from these project activities are discussed and some practical recommendations are identified based on a literature review, interviews and workshops with local government officials and residents. Through those opportunities, it was found that remote regencies around Lake Toba do not have environmentally sound final disposal sites and do not have the financial or technical capacity to provide waste collection and handling services to residents, which often results in illegal dumping and open burning of waste. It was also revealed that lack of established markets for recycling value chains and citizens participation in waste separation at source had resulted in limited success for recycling and 3R activities in the region.


Asunto(s)
Lagos , Administración de Residuos , Indonesia , Organizaciones , Reciclaje
3.
PLoS One ; 16(10): e0258191, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34614026

RESUMEN

Face coverings are a key component of preventive health measure strategies to mitigate the spread of respiratory illnesses. In this study five groups of masks were investigated that are of particular relevance to the SARS-CoV-2 pandemic: re-usable, fabric two-layer and multi-layer masks, disposable procedure/surgical masks, KN95 and N95 filtering facepiece respirators. Experimental work focussed on the particle penetration through mask materials as a function of particle diameter, and the total inward leakage protection performance of the mask system. Geometric mean fabric protection factors varied from 1.78 to 144.5 for the fabric two-layer and KN95 materials, corresponding to overall filtration efficiencies of 43.8% and 99.3% using a flow rate of 17 L/min, equivalent to a breathing expiration rate for a person in a sedentary or standing position conversing with another individual. Geometric mean total inward leakage protection factors for the 2-layer, multi-layer and procedure masks were <2.3, while 6.2 was achieved for the KN95 masks. The highest values were measured for the N95 group at 165.7. Mask performance is dominated by face seal leakage. Despite the additional filtering layers added to cloth masks, and the higher filtration efficiency of the materials used in disposable procedure and KN95 masks, the total inward leakage protection factor was only marginally improved. N95 FFRs were the only mask group investigated that provided not only high filtration efficiency but high total inward leakage protection, and remain the best option to protect individuals from exposure to aerosol in high risk settings. The Mask Quality Factor and total inward leakage performance are very useful to determine the best options for masking. However, it is highly recommended that testing is undertaken on prospective products, or guidance is sought from impartial authorities, to confirm they meet any implied standards.


Asunto(s)
Filtración/instrumentación , Máscaras/estadística & datos numéricos , Respiradores N95/estadística & datos numéricos , Textiles , Equipo Reutilizado , Exposición por Inhalación/prevención & control
4.
Comput Intell Neurosci ; 2021: 2143235, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34603427

RESUMEN

The research on the reverse resource network of e-waste at home and abroad is still in its infancy, and most of it is only based on traditional forward logistics. Reverse resources are the process of moving goods from their typical final destination for recycling value or proper disposal. With the intensification of market competition and the strengthening of environmental protection legislation by the government, reverse resources are no longer a neglected corner in the supply chain. The DLRNN model of the e-waste reverse resource recovery system constructed in this paper can provide an important theoretical and empirical basis for the rational utilization of waste electronic products and fully tap the potential value of waste electronic products, which is of great significance to the recycling of natural resources. In this paper, a hybrid network framework DLRNN based on deep learning (DL) and cyclic neural network (RNN) is designed for problem classification. Experimental results show that the classification accuracy of this framework is improved by 2.4% on TREC and 2.5% on MSQC without additional word vector conversion tools.


Asunto(s)
Residuos Electrónicos , Administración de Residuos , Reciclaje
5.
PLoS One ; 16(9): e0255148, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34492026

RESUMEN

The widespread use of facemasks throughout the population is recommended by the WHO to reduce transmission of the SARS-CoV-2 virus. As some regions of the world are facing mask shortages, reuse may be necessary. However, used masks are considered as a potential hazard that may spread and transmit disease if they are not decontaminated correctly and systematically before reuse. As a result, the inappropriate decontamination practices that are commonly witnessed in the general public are challenging management of the epidemic at a large scale. To achieve public acceptance and implementation, decontamination procedures need to be low-cost and simple. We propose the use of hot hygroscopic materials to decontaminate non-medical facemasks in household settings. We report on the inactivation of a viral load on a facial mask exposed to hot hygroscopic materials for 15 minutes. As opposed to recent academic studies whereby decontamination is achieved by maintaining heat and humidity above a given value, a more flexible procedure is proposed here using a slow decaying pattern, which is both effective and easier to implement, suggesting straightforward public deployment and hence reliable implementation by the population.


Asunto(s)
Descontaminación/métodos , Equipo Reutilizado/normas , Máscaras/virología , COVID-19/prevención & control , Calor , Humanos , Humedad , SARS-CoV-2
6.
J Hazard Mater ; 416: 125972, 2021 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-34492881

RESUMEN

In hydrometallurgy industry, the accumulation of iron removal residues containing heavy metal elements and toxic elements poses great threats to ecological systems. We propose a novel method to prevent the production of hazardous iron removal residues: firstly, neutralization precipitation is used to purify iron ions in solution; after sedimentation of the obtained suspension, only dense underflow is subjected to hydrothermal reaction, in which ferric hydroxide transforms into hematite crystal. Results showed that ferric hydroxide precipitated into a thin sedimentation layer at temperature greater than 60 °C. For hydrothermal treatment of the sedimentation layer, a high hydrothermal reaction temperature was conducive to complete transformation of ferric hydroxide into hematite. The precipitated ferric hydroxide firstly changed from the crystallite of goethite or lepidocrocite to amorphous particles, and then gradually formed spherical α-Fe2O3 monocrystalline with diameter of around 50 nm, as indicated by TEM and XRD results. At 200 °C, hematite precipitates with iron content of about 65% can be obtained. For iron-containing zinc/nickel/cobalt sulfate solution, controlling hydrothermal reaction temperature and acidity of the underflow solution can effectively avoid the generation of zinc/nickel/cobalt hydroxides or subsulfates in the hematite precipitates, thereby significantly reducing the loss of those valuable metals.


Asunto(s)
Hierro , Metales Pesados , Compuestos Férricos , Reciclaje , Zinc
7.
J Hazard Mater ; 416: 126021, 2021 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-34492888

RESUMEN

The meat industry generates a significant amount of hazardous waste, containing phosphorus, calcium, and other elements which could be the basis for other products. This work presents the results of research on the thermal utilisation of bone meat waste and the use of the ash thus obtained as a substitute for phosphorus raw materials. Material Flow Analysis was used to quantify and assess the option with the highest material productivity. Such a solution can be in line with an updated pyramid of food waste hierarchy already proposed in the literature, distinguishing surplus food and a new category for recycling of materials, in analysed case food waste, in the circular economy. The research is based on the example of real data from a Polish meat producer. The quantity of waste from primary production and meat manufacturing containing waste bone in Poland was estimated to be 232,000 t/y (24.0% of the total quantity of meat waste). Its thermal utilisation potentially allows 71,118 t/y of hydroxyapatite ash, a substitute for phosphorites, to be obtained. The high quality hydroxyapatite ash could be used for the production of food grade phosphoric acid and also for the production of food grade mono- and dicalcium feed phosphates.


Asunto(s)
Incineración , Eliminación de Residuos , Ceniza del Carbón , Carne , Fósforo , Reciclaje , Residuos Sólidos
8.
Chimia (Aarau) ; 75(9): 744-751, 2021 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-34526179

RESUMEN

Herein, we describe the status of bio-derived plastics as well as the existing and emerging technologies that are available for their post-consumer end-of-life valorization. We first present how bio-derived plastics can be produced from renewable materials such as biomass and CO2. In the second section, we present an overview of the technologies available for the end-of-life, including pyrolysis and gasification and how they can be leveraged towards a circular economy. We continue the discussion with the presentation of an emerging technology, polyolefin hydrocracking. Finally, the concepts are discussed in light of life cycle analysis that helps to assess the sustainability of manufacture (and recycling) methods.


Asunto(s)
Plásticos , Reciclaje , Biomasa , Muerte , Humanos
9.
Sci Rep ; 11(1): 17680, 2021 09 03.
Artículo en Inglés | MEDLINE | ID: mdl-34480045

RESUMEN

The Covid-19 pandemic led to threatening shortages in healthcare of medical products such as face masks. Due to this major impact on our healthcare society an initiative was conducted between March and July 2020 for reprocessing of face masks from 19 different hospitals. This exceptional opportunity was used to study the costs impact and the carbon footprint of reprocessed face masks relative to new disposable face masks. The aim of this study is to conduct a Life Cycle Assessment (LCA) to assess and compare the climate change impact of disposed versus reprocessed face masks. In total 18.166 high quality medical FFP2 face masks were reprocessed through steam sterilization between March and July 2020. Greenhouse gas emissions during production, transport, sterilization and end-of-life processes were assessed. The background life cycle inventory data were retrieved from the ecoinvent database. The life cycle impact assessment method ReCiPe was used to translate emissions into climate change impact. The cost analysis is based on actual sterilization as well as associated costs compared to the prices of new disposable face masks. A Monte Carlo sampling was used to propagate the uncertainty of different inputs to the LCA results. The carbon footprint appears to be 58% lower for face masks which were reused for five times compared to new face masks which were used for one time only. The sensitivity analysis indicated that the loading capacity of the autoclave and rejection rate of face masks has a large influence on the carbon footprint. The estimated cost price of a reprocessed mask was €1.40 against €1.55. The Life Cycle Assessment demonstrates that reprocessed FFP2 face masks from a circular economy perspective have a lower climate change impact on the carbon footprint than new face masks. For policymakers it is important to realize that the carbon footprint of medical products such as face masks may be reduced by means of circular economy strategies. This study demonstrated a lower climate change impact and lower costs when reprocessing and reusing disposable face masks for five times. Therefore, this study may serve as an inspiration for investigating reprocessing of other medical products that may become scarce. Finally, this study advocates that circular design engineering principles should be taken into account when designing medical devices. This will lead to more sustainable products that have a lower carbon footprint and may be manufactured at lower costs.


Asunto(s)
COVID-19 , Equipo Reutilizado/economía , Máscaras/economía , Pandemias , SARS-CoV-2 , Esterilización/economía , COVID-19/economía , COVID-19/epidemiología , COVID-19/prevención & control , Humanos
10.
Chemosphere ; 282: 131163, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34470177

RESUMEN

Cemented paste backfill (CPB) technology is beneficial to the recycling of solid wastes and sustainable development in mines. However, the wetting-drying cycle caused by water intrusion and goaf drainage has a great influence on the waterproof and support performance of CPB. In this study, the hydraulic and mechanical properties of CPB samples under wetting-drying cycles were studied by permeability tests, nuclear magnetic resonance (NMR) tests and uniaxial compression tests. The results show that with the increasing number of wetting-drying cycles, the porosity and permeability of CPB samples increase; the porosity and permeability of CPB samples with small particle size distribution and cementing strength are more sensitive to the increasing number of wetting-drying cycles. During the wetting-drying cycles, the uniaxial compressive strength and elastic modulus of CPB samples gradually deteriorate. This effect is more significant for CPB samples with the smaller particle size distribution and cementing strength. According to the periodic destruction of CPB cemented structures caused by wetting-drying cycles, the deterioration process of mechanical properties of CPB samples can be divided into two stages: initial deterioration stage and re-deterioration stage. The microstructure analysis of CPB samples was used to verify the periodic destruction of the cemented structures. Finally, protective measures of CPB were proposed to ensure the stability of CPB. The CPB with large particle size distribution and cementing strength should be adopted. Besides, the filling rate and the monitoring condition of the goaf can be strengthend to reduce and even avoid wetting-drying cycles.


Asunto(s)
Residuos Sólidos , Sulfuros , Materiales de Construcción , Minería , Reciclaje
11.
Sheng Wu Gong Cheng Xue Bao ; 37(8): 2688-2702, 2021 Aug 25.
Artículo en Chino | MEDLINE | ID: mdl-34472289

RESUMEN

Plastics are widely used in daily life. Due to poor management and disposal, about 80% of plastic wastes were buried in landfills and eventually became land and ocean waste, causing serious environmental pollution. Recycling plastics is a desirable approach, but not applicable for most of the plastic waste. Microbial degradation offers an environmentally friendly way to degrade the plastic wastes, and this review summarizes the potential microbes, enzymes, and the underpinning mechanisms for degrading six most commonly used plastics including polyethylene terephthalate, polyethylene, polyvinyl chloride, polypropylene, polystyrene and polyurethane. The challenges and future perspectives on microbial degradation of plastics were proposed.


Asunto(s)
Plásticos , Reciclaje , Biodegradación Ambiental , Poliuretanos
12.
Waste Manag Res ; 39(11): 1427-1436, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34494917

RESUMEN

Management of waste is one of the major challenges faced by many developing countries. This study therefore attempts to develop a circular economy (CE) model to manage wastes and closing the loop and reducing the generation of residual wastes in Indian municipalities. Through extant literature review, the researchers found 30 success factors of CE implementation. Using the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) SIMOS approach, the rating and weight of decision makers (DMs) for each factor were collected. A structured questionnaire has been developed incorporating all these 30 factors, to extract the most important factors. The data was collected from top 10 officials (DMs) from the Chennai municipality, who handle three regions (metropolitan, suburbia and industrial). Based on the TOPSIS SIMOS analysis, nine CE implementing factors (critical success factors (CSFs)) among the 30 variables that were significant based on the cut-off value was identified. A CE model has been proposed based on these nine CSFs for waste management in India.


Asunto(s)
Reciclaje , Administración de Residuos , Ciudades , India , Modelos Económicos
13.
J Environ Manage ; 300: 113710, 2021 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-34509811

RESUMEN

The application of green reductant is signification to recycling of cathode materials from spent lithium ions batteries. Here, ginkgo biloba was developed for enhancing leaching of spent LiNi0.6Co0.2Mn0.2O2 materials with systematically analysis of leaching kinetics and interface reaction. The leaching efficiencies of Ni, Mn, Co, and Li reach respectively 98.65 %, 98.25 %, 98.41 % and 99.99 % under optimal condition of 1.8 mol/L H2SO4 concentration, 9 g/L ginkgo biloba, 80 °C leaching temperature, 40 min time and 15 g/L pulp density. The apparent activation energies for leaching of Ni, Co, Mn and Li determined as 74.63, 79.33, 73.14 and 23.43 kJ/mol, respectively, indicates that the leaching process was controlled by the surface chemical reaction during the leaching process. Meanwhile, the regenerated material with better electrochemical performance was obtained by co-precipitation and calcination from leachate. Finally, the process is environmental friendly and economical feasible for recycling of spent lithium-ion batteries.


Asunto(s)
Litio , Sustancias Reductoras , Suministros de Energía Eléctrica , Cinética , Reciclaje
14.
J Environ Manage ; 300: 113700, 2021 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-34517231

RESUMEN

Electroplating sludge is a hazardous waste produced in large quantities in the electroplating industry during production. It is rich in heavy metal resources and can be recovered as value-added heavy metal products. To recover Zn in electroplating sludge, Fe/Al/Ca impurities were effectively removed as hematite, boehmite, and calcium sulfate, respectively, via a facile hydrothermal method with reduction of nitric acid by addition of glucose. After the sludge was dissolved in nitric acid, the generated solution contained 6.1 g/L of Zn, 2.2 g/L of Fe, 2.5 g/L of Al, and 2.9 g/L of Ca. First, approximately 100% Fe was extracted as hematite nanoparticles containing 94.6 wt% Fe2O3 after the solution was treated at 190 °C for 6 h. Second, when the temperature was elevated to 270 °C, nearly 99% Al was isolated as boehmite particles containing 95.2 wt% Al2O3. Third, more than 98% Ca was removed as anhydrite, which contained 95.9 wt% CaSO4, by adding sulfuric acid. During the steps, the total loss of Zn was less than 3%, and 5.75 g/L of residual Zn was recovered as zincite containing 92.2 wt% ZnO by adjusting the pH to 8. The dissolved Fe, Al, and Ca impurities were successfully removed as purified hematite, boehmite, and anhydrite, respectively, through the stepwise separation method by adjusting reaction temperatures and pH. The high content of Zn in the electroplating sludge was finally purified as zincite.


Asunto(s)
Metales Pesados , Aguas del Alcantarillado , Galvanoplastia , Reciclaje , Zinc
15.
J Environ Manage ; 300: 113714, 2021 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-34547570

RESUMEN

Solid blasting waste generated from coating removal during ship repair and maintenance poses environmental challenges. This paper presents a review of the generation, characterization, and treatment of blasting waste in the ship repair industry. The quantities, properties, and environmental impacts of the generated blasting waste are summarized and analyzed, and the results indicate that blasting waste has a high generation rate and/or high toxicity. As alternatives to landfill, available blasting waste treatment methods include hydrometallurgical leaching, physical/physicochemical separation, thermal treatment, and direct utilization in the production of building materials. The advantages and disadvantages of these treatments are reviewed and compared. The production of building materials from blasting waste is currently attractive owning to its economic benefits and technical simplicity, whereas recycling blasting waste for high-value applications is gradually gaining research interest. The high dependence of the choice and performance of treatment methods on the waste type and characteristics is highlighted. The results emphasize the interest in conducting more research on physiochemical and thermal properties and the environmental effects of blasting waste. This review suggests that future research should focus more on paint waste management because of the restricted application of dry abrasive blasting and the development of nonabrasive blasting methods in the ship repair industry.


Asunto(s)
Navíos , Administración de Residuos , Conservación de los Recursos Naturales , Materiales de Construcción , Pintura , Reciclaje , Residuos Sólidos
16.
J Environ Manage ; 300: 113781, 2021 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-34560466

RESUMEN

This work presents a novel concept for the integration of closed-circuit reverse osmosis (CCRO) technology and solvent-based precipitation as a means of producing an exceptional quality of water by separating the salts especially chlorides and sulphates from highly saline nanofiltration (NF) rejected stream of the steel industry. The NF rejected stream was extremely concentrated with salts like chloride (1560 mg/L), sulphate (4212 mg/L), manganese (28 mg/L), sodium (418 mg/L) and total dissolved solids (TDS), as high as 8100 mg/L, which are well above the permissible limit for surface discharge. The outcome of this work showed that reverse osmosis (RO) with continuous brine recycling achieved excellent desalination performance. Miscible organic solvents such as diisopropylamine (DIIPA), isopropylamine (IPA), and ethylamine (EA) were found to be effective in precipitating chloride and sulphate ions from highly concentrated RO brine. The overall removal efficiency of sulphate and chloride was found to be 99.88% and 91%, respectively. Preliminary treatment cost was estimated and found to be around 7.35 $/m3. The treated water can either be recycled in the system or safely released into the environment. The readers of this research article will be benefitted by gaining a thorough understanding of the treatment of concentrated brine from nanofiltration using an integrated RO-precipitation technique.


Asunto(s)
Purificación del Agua , Filtración , Membranas Artificiales , Ósmosis , Reciclaje , Acero
17.
Sci Rep ; 11(1): 18316, 2021 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-34526550

RESUMEN

Shortages of personal protective equipment for use during the SARS-CoV-2 pandemic continue to be an issue among health-care workers globally. Extended and repeated use of N95 filtering facepiece respirators without adequate decontamination is of particular concern. Although several methods to decontaminate and re-use these masks have been proposed, logistic or practical issues limit adoption of these techniques. In this study, we propose and validate the use of the application of moist heat (70 °C with humidity augmented by an open pan of water) applied by commonly available hospital (blanket) warming cabinets to decontaminate N95 masks. This report shows that a variety of N95 masks can be repeatedly decontaminated of SARS-CoV-2 over 6 h moist heat exposure without compromise of their filtering function as assessed by standard fit and sodium chloride aerosol filtration efficiency testing. This approached can easily adapted to provide point-of-care N95 mask decontamination allowing for increased practical utility of mask recycling in the health care setting.


Asunto(s)
Descontaminación/métodos , Respiradores N95/virología , SARS-CoV-2/fisiología , Equipo Reutilizado , Hospitales , Humanos , Humedad , Sistemas de Atención de Punto , Factores de Tiempo , Inactivación de Virus
18.
Waste Manag Res ; 39(10): 1218-1236, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34525879

RESUMEN

Solid waste management (SWM) is an essential utility service. More than two to three billion people worldwide still lack basic services, whereas some countries are already moving beyond SWM towards waste and resource management (WaRM) and a circular economy. This paper sets out a novel conceptual framework and global theory of waste and development, providing a road map, allowing a country or city to locate their current position and plot their way ahead. We identify nine development bands (9DBs) with significant commonalities in terms of critical challenges and developmental pressure points. DB1-DB4 reflect stepwise improvement towards the new baseline of meeting the SDG 11.6.1 indicators of universal collection and management in controlled facilities (DB5). Countries can then choose to move towards environmentally sound management and the 'reduce, reuse, recycle' (3Rs) (DB6-9), with an ultimate aspiration of 'zero waste'. We test the 9DBs conceptual framework against historical journeys of higher income countries. The main application will be in low- and middle-income countries striving towards SDG 11.6.1, where it fills a key gap in the practitioners' toolkit by enabling initial framing/scoping of the problem and smarter interventions to be designed and sense checked. Key insights include targeted governance/institutional reforms, appropriate and affordable systems/technology and adapting solutions to a diversity of local needs and realities.


Asunto(s)
Eliminación de Residuos , Administración de Residuos , Ciudades , Humanos , Reciclaje , Residuos Sólidos/análisis
19.
Waste Manag Res ; 39(10): 1302-1316, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34581643

RESUMEN

Waste electrical and electronic equipment (WEEE) represent one of the growing waste streams in Europe, whose content of hazardous substances and valuable materials focus on how it is handled. However, there are differences between countries regarding e-waste management system in Europe. This paper analyses and presents data regarding the e-waste management system in Italy, one of the founding countries of the EU and Serbia - EU candidate country. Within this work, the following aspects were considered: legislative framework and EU directive goals in terms of e-waste, institutional setup, collection system, and existing recycling and treatment technologies. In addition, material flow analysis is used to model mass balance within WEEE treatment plants in both countries. Finally, through assessment and comparison of current systems in both countries, problems and shortages of Serbia's e-waste management system are addressed.


Asunto(s)
Residuos Electrónicos , Administración de Residuos , Residuos Electrónicos/análisis , Electrónica , Italia , Reciclaje , Serbia
20.
Mol Biol Rep ; 48(10): 6871-6877, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34510319

RESUMEN

BACKGROUND: RT-qPCR technique is the current world-wide method used for the early detection of SARS-CoV2 RNA in the suspected clinical samples. Viral RNA extraction is the key pre-analytical step for SARS-CoV2 detection which often achieved using commercial RNA-extraction kits. However, due to the COVID-19 pandemic, bulk production and the supply chains for the commercial RNA-extraction kit have been seriously compromised. The shortage of commercial RNA-extraction kit is even more acute in developing country. Furthermore, use of one-off design RNA-columns can generate plastic wastes that have an environmental pollution effect. METHODS AND RESULTS: To address these issues, in this study, we used warm alkaline solution containing Triton X-100 for the complete removal of the residual SARS-CoV2 RNA from the used RNA-binding silica column. Columns regenerated using the alkaline solution have the viral RNA purification capability that is comparable to the fresh silica columns. We also demonstrated that RNA-binding silica columns can be regenerated and reused for a minimum of five-times. CONCLUSIONS: Therefore, the use of the RNA-column regeneration method may benefits several SARS-CoV2 diagnostic laboratories throughout the world by cutting down the requirement of commercial RNA-purification column.


Asunto(s)
Prueba de Ácido Nucleico para COVID-19/instrumentación , Cromatografía/instrumentación , ARN Viral/aislamiento & purificación , Prueba de Ácido Nucleico para COVID-19/métodos , Cromatografía/métodos , Humanos , Octoxinol , ARN Viral/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Reciclaje , Dióxido de Silicio
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