A combined qualitative and quantitative method development and validation of vancomycin hydrochloride injection formulation by HPLC and UV involving quality by design.

A selective, specific, precise, linear, accurate and robust analytical method was developed and validated for the assay of vancomycin HCl in vancomycin hydrochloride injection. Comparative UV spectrophotometric and reverse-phase HPLC were used to develop the quantitative determination. Acetonitrile and pH 2.2 phosphate buffer in the ratio 20:80 v/v were used as the mobile phase, and a flow rate of 1.0 ml/min with a 20 min run time. The detection was carried out at 235 nm with a Nucleosil C18 (250 × 4.6 mm) 10 µm column, and the ambient column temperature was maintained. The method uses a 20 µl injection volume and diluent as a blank solution in this connection. The method was validated as per the current regulatory guidelines. The linearity of this method was found to be linear in the range of 50-150% of the working concentration, and the correlation coefficient was >0.999. The method's accuracy was within the acceptable range, which was 98.1-101.5%. The method's precision was within an acceptable range of about 0.32% RSD. The analytical solution was stable for up to 48 h at room temperature. The method's robustness was proved by utilizing quality design tools. Stress studies demonstrated the method's stability-indicating nature.

Optimization of LC-MS2 Data Acquisition Parameters for Molecular Networking Applied to Marine Natural Products.

Since the introduction of the online open-source GNPS, molecular networking has quickly become a widely applied tool in the field of natural products chemistry, with applications from dereplication, genome mining, metabolomics, and visualization of chemical space. Studies have shown that data dependent acquisition (DDA) parameters affect molecular network topology but are limited in the number of parameters studied. With an aim to optimize LC-MS2 parameters for integrating GNPS-based molecular networking into our marine natural products workflow, a design of experiment (DOE) was used to screen the significance of the effect that eleven parameters have on both Classical Molecular Networking workflow (CLMN) and the new Feature-Based Molecular Networking workflow (FBMN). Our results indicate that four parameters (concentration, run duration, collision energy and number of precursors per cycle) are the most significant data acquisition parameters affecting the network topology. While concentration and the LC duration were found to be the two most important factors to optimize for CLMN, the number of precursors per cycle and collision energy were also very important factors to optimize for FBMN.

DOE JGI Metagenome Workflow.

The DOE Joint Genome Institute (JGI) Metagenome Workflow performs metagenome data processing, including assembly; structural, functional, and taxonomic annotation; and binning of metagenomic data sets that are subsequently included into the Integrated Microbial Genomes and Microbiomes (IMG/M) (I.-M. A. Chen, K. Chu, K. Palaniappan, A. Ratner, et al., Nucleic Acids Res, 49:D751-D763, 2021, https://doi.org/10.1093/nar/gkaa939) comparative analysis system and provided for download via the JGI data portal (https://genome.jgi.doe.gov/portal/). This workflow scales to run on thousands of metagenome samples per year, which can vary by the complexity of microbial communities and sequencing depth. Here, we describe the different tools, databases, and parameters used at different steps of the workflow to help with the interpretation of metagenome data available in IMG and to enable researchers to apply this workflow to their own data. We use 20 publicly available sediment metagenomes to illustrate the computing requirements for the different steps and highlight the typical results of data processing. The workflow modules for read filtering and metagenome assembly are available as a workflow description language (WDL) file (https://code.jgi.doe.gov/BFoster/jgi_meta_wdl). The workflow modules for annotation and binning are provided as a service to the user community at https://img.jgi.doe.gov/submit and require filling out the project and associated metadata descriptions in the Genomes OnLine Database (GOLD) (S. Mukherjee, D. Stamatis, J. Bertsch, G. Ovchinnikova, et al., Nucleic Acids Res, 49:D723-D733, 2021, https://doi.org/10.1093/nar/gkaa983).IMPORTANCE The DOE JGI Metagenome Workflow is designed for processing metagenomic data sets starting from Illumina fastq files. It performs data preprocessing, error correction, assembly, structural and functional annotation, and binning. The results of processing are provided in several standard formats, such as fasta and gff, and can be used for subsequent integration into the Integrated Microbial Genomes and Microbiomes (IMG/M) system where they can be compared to a comprehensive set of publicly available metagenomes. As of 30 July 2020, 7,155 JGI metagenomes have been processed by the DOE JGI Metagenome Workflow. Here, we present a metagenome workflow developed at the JGI that generates rich data in standard formats and has been optimized for downstream analyses ranging from assessment of the functional and taxonomic composition of microbial communities to genome-resolved metagenomics and the identification and characterization of novel taxa. This workflow is currently being used to analyze thousands of metagenomic data sets in a consistent and standardized manner.

Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits.

Sixty-four nulliparous female rabbits were distributed among eight groups (eight animals/group). Group one was the unsupplemented control group; the other seven groups were supplemented with zinc bacitracin (ZnB) at 100 mg, or bee pollen (BP) and/or propolis (Pro) at 150 and 300 mg in a capsulated form, three times a week, day after day, continuously all over the experimental period. The experiment was run for eight parties; at each parity, 28 kids of each doe group (a total of 224 rabbits) were divided into two subgroups weaned, respectively, at 24 and 30 days of age. Thus, for each parity, there were 16 groups (eight does treatments × two weaning age, 14 rabbits per group). The growing rabbits fed the standard diets without supplements. The growth performance, the carcass traits, the liver and the spleen histology of rabbits were checked up to 90 days of age to find possible carryover effects of the supplements. The supplements had no significant effect on most of the growth performance at 90 days of age, but BP150 and BP+Pro300 increased the growth rate in comparison with ZnB group. The liver weight in the control, BP300 and Pro300 groups was higher than the ZnB one. The spleen weight was higher in the groups ZnB, BP150, Pro300 and BP+Pro300, followed by the control, BP300 and BP+Pro150 and thus Pro150. The heart % in the BP150 and Pro300 groups was higher than ZnB and BP+Pro150 groups. A lymphoid hyperplasia of splenic white pulp was observed in the BP+Pro groups, while propolis alone showed a mild activation of lymphobiosis. The Pro and BP groups showed the same picture of the control group exhibiting a hydropic degeneration of mostly hepatic cells, while the ZnB group exhibited adverse effect on the bile ducts featuring portal periductal inflammatory cells infiltration with epithelial hyperplasia reflecting chronic cholangitis.

Capacidad ventilatoria forzada en trabajadores de una empresa metalúrgica en la Región Central del Perú 2012 / Forced ventilatory capacity in workers of a metallurgical company in the central region of Peru 2012

El estudio se realizó en la ciudad de La Oroya, capital de la Provincia de Yauli, en el Departamento de Junín a 3730 m.s.n.m., en las instalaciones del Centro Médico de Chulec perteneciente a la Empresa Doe Run Perú S.R.L. en trabajadores de la fundición del Complejo Metalúrgico, quienes participaron en forma voluntaria. Objetivo: Determinar la capacidad ventilatoria de los trabajadores de una empresa metalúrgica que laboran en zona de altura en la región central del Perú; según los indicadores espirométricos siguientes: Capacidad Vital Forzada (CVF), Volumen Espiratorio Forzado en el primer segundo (VEF1), relación entre Volumen Espiratorio Forzado en el primer segundo sobre la Capacidad Vital Forzada (VEF1/CVF), y el Flujo Espiratorio Pico de las siglas en inglés (PEF). La investigación servirá de aproximación a los valores de referencia, para trabajadores en zona de altura en el Perú. Metodología: Se realizó un estudio observacional, descriptivo y transversal en 385 trabajadores nativos o con un tiempo de residencia igual o mayor a 5 años, cuyas edades fueron entre 20 a 60 años, y a quienes se les practicó la prueba de espirometría forzada para determinar la capacidad funcional respiratoria, en base a los criterios de la Sociedad Americana de Tórax (ATS). Resultados: Los valores fueron los siguientes: Capacidad Vital Forzada: 4.83 l/s, el Volumen Espiratorio Forzado al primer segundo fue de: 4.02 l/s, la relación FEV1/FVC fue de 83.45 por ciento, y el Flujo Espiratorio Pico fue de 9.46 l/s. Conclusión: El 94.6 por ciento de los trabajadores evaluados, la Capacidad Vital Forzada estaba dentro de rangos normales. Un 5.2 por ciento de la población estudiada es portadora de procesos obstructivos leves, y el 0.2 por ciento es portadora de probable proceso restrictivo...

This study was conducted in the city of La Oroya, capital of the Province of Yauli, Department of Junín located at 3730 meters above sea level. The study took place in the installations of the Medical Center Chulee which belongs to the company Doe Run Peru SRL. The workers of the Metallurgical Complex voluntarily participated. Objective: To determine the ventilatory capacity of workers in a metallurgical company performing their work at high altitudes in the central region of Peru. According to the following spirometric indicators: Forced Vital Capacity (FVC), forced expiratory volume in one second (FEV1), forced expiratory volume ratio in the first second of the FVC (FEV1) / FVC, and peak expiratory flow the acronym (PEF). The research will approach the reference values for workers in the highlands in Peru. Methodology: An observational, descriptive and cross-sectional study involved 385 native workers with a residence time equal to or more than five years. The ages were between 20-60 years and the participants underwent spirometry testing to determine the respiratory function, based on the criteria of the American Thoracic Society (ATS). Results: The values were the following: Forced Vital Capacity, FVC: 4.83 l/s, the forced expiratory volume at the first second was 4.02 l/s, The FEV1/FVC ratio was 83.45 per cent, and the peak expiratory flow was 9.46 l/s. Conclusion: 94.6 per cent of workers evaluated showed their FVC within normal ranges. 5.2 per cent of the studied population are carriers of mild obstructive processes, and 0.2 per cent are carriers of a likely restrictive process...