First report of potato virus S and potato virus Y in tomatoes from Croatia.

Potato virus Y (PVY, genus Potyvirus) is an economically important aphid-transmissible virus with a very wide host range reported in many tomato-growing areas (Rivarez et al. 2021). Potato virus S (PVS, genus Carlavirus) has a limited host range (Lin et al. 2014) and occurs in tomato (Predajna et al. 2017), mostly in mixed infections with other viruses. In 2021, greenhouse tomatoes from Vidovec (46° 17' 3.4'' N, 16° 15' 37.0'' E) in the northwestern and Sedlarica (45° 54' 23.0'' N, 17° 12' 0.5'' E) in the eastern regions of Croatia were surveyed for virus-like diseases. In total, 30 plants were sampled (12 from Vidovec and 18 from Sedlarica) showing symptoms of mild mottling, leaf rugosity and mild bronzing followed by leaf necroses later in the season. Nucleic acids were extracted from leaves by adapted CTAB procedure (Murray and Thompson 1980) and DNase treated. Four representative samples from Vidovec and four from Sedlarica were pooled for high throughput sequencing (HTS). After rRNA depletion (RiboMinus™ Plant Kit for RNA-Seq, Invitrogen) and polyA tailing, two location specific libraries (PCR-cDNA sequencing kit, Oxford Nanopore Technologies) were prepared for nanopore HTS on MinION Mk1C device. From Vidovec samples, 459,285 raw reads (mean length 354 nt) were obtained and 206,718 (mean length 446 nt) from Sedlarica and mapped (Minimap2, v.2.17) against Kraken2 viral genome sequences database (https://benlangmead.github.io/aws-indexes/k2). The number of reads mapped to PVS genome was 1004 from Vidovec (coverage depth 1.56) and those mapped to PVY genome were 781 (coverage depth 0.99) and 57 (coverage depth 1), from Vidovec and Sedlarica, respectively. The PVS complete consensus genome from Vidovec (ON468562, 8485 nt) had 99.09% nucleotide identity (BLASTn) to a potato isolate from the Netherlands (MF418030). The PVY consensus genome sequences from Vidovec (ON505007, 9698 nt) and Sedlarica (ON505008, 9698 nt) had respectively 98.37% and 98.48% identities to a tomato isolate from Slovakia (MW685827). Reverse transcription polymerase chain reaction (RT-PCR) was performed for all 30 samples and amplicons were Sanger sequenced, with primers PVS-7773F/PVS-3'endR for a 720 nt PVS genome portion spanning the 3'-part of the CP and a complete 11K gene (Lin et al. 2014) and PVY-2F/2R primers for a 510 nt portion of PVY CP gene (Aramburu et al. 2006). Only one tomato out of 12 ('Borana') from Vidovec harbored PVS in the mixed infection with PVY. Two additional tomatoes from Vidovec and two from Sedlarica were infected solely by PVY. Amplicon sequences of PVS (ON651427) and PVY (ON707000-4, ON734067-8) had 100% identity with the HTS assembled sequences. The PVS isolate from Croatia grouped with PVSO (ordinary) strain in phylogenetic analysis and the PVY isolates from both sites grouped with the PVY-NTN strain (Cox and Jones 2010). Although PVY is considered to be widespread in tomato (Nikolic et al. 2018; Rivarez et al. 2021), this is its first report from Croatia. PVS, newly reported from Croatia here, is probably not associated with the symptoms recorded because the same symptomatology was observed in the singly and mixed infected 'Borana' tomato plants. The occurrence of PVY in the geographically distant (100 km apart) Vidovec and Sedlarica, suggests that it is widespread in the continental Croatia where tomatoes are commercially grown in plastic greenhouses. Further analyses are needed to elucidate PVY and PVS epidemiology and impact on the local tomato production.

Influence of Electronic Devices on Sleep and Cognitive Performance During Athlete Training Camps.

ABSTRACT: Jones, MJ, Dawson, B, Eastwood, PR, Halson, SL, Miller, J, Murray, K, Dunican, IC, Landers, GJ, and Peeling, P. Influence of electronic devices on sleep and cognitive performance during athlete training camps. J Strength Cond Res 35(6): 1620-1627, 2021-This study investigated the effects of removing athletes' electronic devices in the evening on sleep and performance during training camps. Water polo athletes (n = 26) attending a 7-night training camp (study 1) and triathletes (n = 23) attending a 4-night training camp (study 2) were randomly allocated to a no-device group (no electronic devices could be used after dinner or overnight; ND) or control group (unrestricted electronic device use; CON). Sleep was monitored through wrist actigraphy. The ND group completed a questionnaire measuring anxiety related to being unable to use electronic devices ("nomophobia"). Triathletes also completed a psychomotor vigilance test (PVT) at the start and end of camp. Water polo ND athletes went to bed earlier and spent longer time in bed than CON on the first night, but not on other nights. In triathletes, sleep quantity was not different between groups on any night. No statistically significant differences were observed for changes in nomophobia from the first to the last night of camp. No differences in PVT performance were observed between ND and CON triathletes. In conclusion, removal of evening electronic devices does not improve sleep quantity or cognitive performance in athletes during short-duration (4-7 nights) training camps.

Physical Characteristics of Elite Youth Female Soccer Players Characterized by Maturity Status.

Emmonds, S, Scantlebury, S, Murray, E, Turner, L, Robsinon, C, and Jones, B. Physical characteristics of elite youth female soccer players characterized by maturity status. J Strength Cond Res 34(8): 2321-2328, 2020-The purpose of this study was to investigate the influence of maturity status on the physical characteristics of youth female soccer players. One hundred fifty-seven players from 3 elite soccer academies in England completed assessments of anthropometry, strength (isometric midthigh pull), lower-body power (countermovement jump [CMJ]), aerobic capacity (Yo-Yo intermittent recovery test level 1), change of direction (CoD: 505-left/right), and speed (10 and 30 m). Each player was classified into 1 of 6 maturity groups based on their estimated years from peak height velocity (YPHV). Magnitude-based inferences were used to assess for the practical significance between consecutive groups. Speed, CoD time, CMJ, and aerobic capacity were all possibly most likely better in more mature players. However, there was a likely difference in relative peak force between maturity groups -0.5 YPHV (27.13 ± 4.24 N·Kg) and 0.5 YPHV (24.62 ± 3.70 N·Kg), which was associated with a likely difference in 10-m sprint time (-0.5 YPHV: 2.00 ± 0.12 vs. 0.5 YPHV 2.08 ± 0.16 seconds) and unclear changes in CMJ and CoD time. Findings provide novel comparative data for this cohort relative to maturity status and can be used by strength and conditioning coaches to inform the design of training programs for youth female soccer players. Strength and conditioning coaches should be aware that youth female soccer players may experience a decrease in relative strength around peak height velocity, which may impact upon the speed, CoD time, and CMJ of players.

A 4-Year Profile of Game Demands in Elite Women's Division I College Basketball.

Ransdell, LB, Murray, T, Gao, Y, Jones, P, and Bycura, D. A 4-year profile of game demands in elite women's Division I college basketball. J Strength Cond Res 34(3): 632-638, 2020-Workload for a Division I women's collegiate basketball team (0.817 win percentage) was examined by: (a) season, (b) player position, and (c) game outcome (wins vs. losses). Female athletes (n = 6, mean 19.7 ± 1.5 years, at beginning of study) wore Catapult S5 units during 91.8% of games over a 4-year period. Average PlayerLoad, PlayerLoad per minute (PL·min), high inertial movement analysis (high-IMA), and jumps were quantified using Catapult Openfield software (version 1.14.1+). Data were checked for normality and log- or square-root-transformed when they were non-normal. A series of linear mixed model analyses were conducted to detect differences in PlayerLoad, PL·min, high-IMA, and jumps by season, position, and game outcome. PL·min and jumps data were not normal, so they were transformed, analyses were run; because there were no differences in findings, data are reported in original units to allow for comparisons with other studies. Cohen's d and confidence intervals were provided as additional information about the strength of reported differences. The 3 most consistent findings were that across a 4-year period, jumps increased, PL·min was higher in guards compared with posts, and high-IMA was higher in losses compared with wins. Other workload patterns were inconsistent, and inappropriate for making conclusive statements. Therefore, comparing jumps across multiple seasons, PL·min by player position and high-IMA in losses are important; in addition, all data can be used to profile National Collegiate Athletic Association Division I women's basketball players and set game workload expectations.

POSTPARTUM BONDING DIFFICULTIES AND ADULT ATTACHMENT STYLES: THE MEDIATING ROLE OF POSTPARTUM DEPRESSION AND CHILDBIRTH-RELATED PTSD.

Despite decades of research demonstrating the role of adult attachment styles and early mother-infant bonding in parenting behaviors and maternal mental health, these constructs have seldom been studied together. The present study aimed to investigate the relationship between attachment styles and specific bonding difficulties of mothers. In addition, as postpartum depression and childbirth-related posttraumatic stress symptoms have been associated with both constructs, we explored their possible mediation effect. One hundred fourteen mothers, 4 to 12 weeks' postpartum, completed a demographic questionnaire, the Adult Attachment Style Questionnaire (M. Mikulincer, V. Florian, & A. Tolmacz, 1990), the Postpartum Bonding Questionnaire (L.F. Brockington, C. Fraser, & D. Wilson, 2006), the Modified Perinatal Posttraumatic Stress Disorder Questionnaire (J.L. Callahan, S.E. Borja, & M.T. Hynan, 2006), and the Edinburgh Postnatal Depression Scale (J.L. Cox, G. Chapman, D. Murray, & P. Jones, 1996), using an online survey system. As predicted, insecure attachment styles were associated with bonding difficulties wherein anxious/ambivalent attachment was associated with greater infant-focused anxiety, mediated by postpartum depression but not childbirth-related PTSD symptoms. In contrast, greater avoidant attachment style was associated with greater rejection and anger, mediated by childbirth-related posttraumatic stress disorder (PTSD), but not depression symptoms. The current study confirmed the association of different attachment styles with bonding as well as the mediating roles of childbirth-related PTSD and postpartum depression symptoms. Future psychological interventions may utilize such evidence to target interventions for bonding disorders in accordance with individual differences.

EXAMINING PARENTS' ROMANTIC ATTACHMENT STYLES AND DEPRESSIVE AND ANXIETY SYMPTOMS AS PREDICTORS OF CAREGIVING EXPERIENCES.

Evidence has suggested that parental romantic attachment style and depressive and anxiety symptoms are related to experiences of caregiving (Creswell, Apetroaia, Murray, & Cooper, 2013; Jones, Cassidy, & Shaver, 2014; Lovejoy, Graczyk, O'Hare, & Neuman, 2000), but more research is necessary to clarify the nature of these relations, particularly in the context of attachment-salient events such as reunions. In a cross-sectional study of 150 parents of children ages 1 to 3 years, we assessed participants' attachment styles (self-reported anxiety and avoidance) and depressive and anxiety symptoms. Participants generated a narrative describing their most recent reunion with their child, which we coded for caregiving outcomes of negative emotion and secure base script content. Attachment style and depressive and anxiety symptoms separately predicted each caregiving outcome. Depressive and anxiety symptoms mediated the associations between attachment style and caregiving outcomes. These results suggest that parental attachment insecurity and depressive and anxiety symptoms contribute to negative emotion and reduced secure base script content. Further, depressive and anxiety symptomatology partially accounts for the relation between attachment insecurity and caregiving outcomes, suggesting that parental mental health is a critical point for intervention.

Exploring indigenous health using the clinical reasoning cycle: a student paper.

As a requirement for a third year nursing subject based on Australian Indigenous Health and Community Nursing, an assessment was undertaken based on the clinical reasoning cycle. The clinical reasoning cycle refers to clinical judgements and clinical decision-making which can be conceptualised as a logical process of cue collection, processing information, understanding the situation, planning and implementing interventions, evaluating outcomes and reflecting on the process (Levett-Jones et al., 2009). The clinical reasoning cycle for this assessment relates to interactions with Murray (pseudonym), an Australian Indigenous male being treated for alcohol dependence within a community setting. This paper will explore the nursing care of Murray through the clinical reasoning cycle, focusing on community-based, culturally appropriate nursing care.

Structural and functional characterization of nonstructural protein 2 for its role in hepatitis C virus assembly.

The hepatitis C virus (HCV) is a flavivirus replicating in the cytoplasm of infected cells. The HCV genome is a single-stranded RNA encoding a polyprotein that is cleaved by cellular and viral proteases into 10 different products. While the structural proteins core protein, envelope protein 1 (E1) and E2 build up the virus particle, most nonstructural (NS) proteins are required for RNA replication. One of the least studied proteins is NS2, which is composed of a C-terminal cytosolic protease domain and a highly hydrophobic N-terminal domain. It is assumed that the latter is composed of three trans-membrane segments (TMS) that tightly attach NS2 to intracellular membranes. Taking advantage of a system to study HCV assembly in a hepatoma cell line, in this study we performed a detailed characterization of NS2 with respect to its role for virus particle assembly. In agreement with an earlier report ( Jones, C. T., Murray, C. L., Eastman, D. K., Tassello, J., and Rice, C. M. (2007) J. Virol. 81, 8374-8383 ), we demonstrate that the protease domain, but not its enzymatic activity, is required for infectious virus production. We also show that serine residue 168 in NS2, implicated in the phosphorylation and stability of this protein, is dispensable for virion formation. In addition, we determined the NMR structure of the first TMS of NS2 and show that the N-terminal segment (amino acids 3-11) forms a putative flexible helical element connected to a stable alpha-helix (amino acids 12-21) that includes an absolutely conserved helix side in genotype 1b. By using this structure as well as the amino acid conservation as a guide for a functional study, we determined the contribution of individual amino acid residues in TMS1 for HCV assembly. We identified several residues that are critical for virion formation, most notably a central glycine residue at position 10 of TMS1. Finally, we demonstrate that mutations in NS2 blocking HCV assembly can be rescued by trans-complementation.

First Report of Cephalosporium gramineum, Causal Agent of Cephalosporium Stripe of Wheat, in a Commercial Winter Wheat Field in Virginia.

Cephalosporium stripe (CS) (2) was identified in a commercial field of winter wheat (Triticum aestivum) near Riner, Montgomery County, Virginia in May 2006. Nearly 15% of the field was severely affected. Broad, yellow-brown stripes were observed on the leaf blades of affected plants, and many plants were stunted and had ripened prematurely. Symptomatic plants were associated with low acidic (pH 5.2), wet spots of the field. Leaves and nodes of affected plants were surface disinfested for 1 min in 5% sodium hypochlorite, plated on corn meal agar (CMA), and incubated at 20°C for 5 days. Cephalosporium gramineum was isolated from numerous plants. Cultures of the fungus produced hyaline conidiophores approximately 5 µm long and unicellular conidia 3 to 7 µm long. Aqueous suspensions of mycelia and conidia were prepared from pure cultures. Several spring wheat cultivars were wounded by severing the root mass and were inoculated when the fifth stem node was detectable (35 on Zadoks scale). Noninoculated plants were wounded as controls. Plants were kept in the greenhouse at temperatures of 22 to 27°C. After 14 days, inoculated plants produced symptoms of CS, and the fungus was reisolated from the leaves of these plants. No symptoms were observed on noninoculated control plants. Though CS had been observed in Virginia in research nurseries (1), to our knowledge, this is the first confirmed report of the disease in a commercial wheat field in Virginia. References: (1) J. B. Jones et al. Plant Dis. 64:325, 1980. (2) C. M. Stiles and T. D. Murray. Phytopathology 86:177, 1996.

The use of maggots as a new treatment in the community.

Since maggots were reintroduced into modern wound management practice, published papers have described their mode of action in the treatment of wounds such as pressure sores and leg ulcers (Thomas et al, 1996a; Thomas et al, 1996b), diabetic ulcers (Jones and Thomas, 2000; Johnson, 1999; Murray and Benbow, 1999; Rayman et al, 1998; Evans, 1997; Mumcuoglu et al, 1997; Thomas et al, 1996a), traumatic injuries (Thomas et al, 1996b), burns (Namias et al, 2000) and surgical wounds (Fear et al, 2003; Jones and Champion, 1998; Young, 1997).

Trends in stature in the South Australian Aboriginal Murraylands.

Millennial and secular changes in body height of prehistoric and recent Aboriginal South Australians are investigated. Skeletal remains of 55 male and 40 female individuals who were excavated at Roonka on the River Murray were dated from 9800 to 100 years BP. Stature was reconstructed by using humerus, femur, and tibia ratios to stature derived from Abbie's (1975) data on living Aborigines and the Trotter-Gleser method for blacks. The respective averages were 1,652 mm and 1,665 mm for males and 1,527 mm and 1,549 mm for females. In 1996/1997, statures of 27 adult males and 21 adult females were measured in Aboriginal centers of Gerard and Raukkan (Point McLeay) on the Lower River Murray. These people, as far as it can be ascertained, are the descendants of the people from Roonka. Their statures were adjusted for the stature loss with age, so that the data represent young individuals (< or = 30 years of age). The average male stature was 1,712 mm, and the average female stature was 1,567 mm. Data collected by Wood Jones and Campbell in 1924 for Aboriginal South Australians show that young adult male stature was 1,668 mm (n=6), and female stature was 1,552 mm (n=4). Slopes of regressions of individual statures on radiocarbon dates and on dates of birth are not significantly different from zero. The same is true for regressions of individual long bone lengths on radiocarbon dates. It can be concluded that there was little change in stature of Aboriginal South Australians from prehistoric to recent times. Regressions of individual age-corrected heights on birth dates (1860-1980) of Aboriginal men and women measured in 1924 and in 1996 further indicate no significant increase in height in either sex.

Jones v. Murray.

KIE: The U.S. Court of Appeals, Fourth Circuit, upheld Virginia legislation requiring convicted felons to provide blood samples for a DNA bank designed to make future criminal identifications using DNA analysis. The court noted that lawfully arrested prisoners' Fourth Amendment rights were not violated because blood testing incarcerated felons for future identification is justified where governmental interest prevails over the "minor" intrusion. In addition, the right of privacy from routine searches of a prisoner's body cavity or jail cell is lost because their identity has become a legitimate state interest. The court limited its support, holding that such authority may not be used against mandatory parolees if prisoners refuse to comply.^ieng

Basal-body-associated disks are additional structural elements of the flagellar apparatus isolated from Wolinella succinogenes.

The intact flagella of Wolinella succinogenes, a gram-negative, anaerobic bacterium with a single polar flagellum, were obtained by an improved procedure, introduced recently by Aizawa et al. (S.-J. Aizawa, G. E. Dean, C. J. Jones, R. M. Macnab, and S. Yamaguchi, J. Bacteriol. 161:836-849, 1985) for the flagellum of Salmonella typhimurium. Disks with a diameter of 130 +/- 30 nm, which were attached to the basal body of the isolated intact flagella, could be identified by electron microscopy as additional structural elements of the bacterial flagellar apparatus. In freeze-dried and metal-shadowed samples, two rings of the basal body were detected on one side and a terminal knob was located on the other side of the disks. Suspension of the flagellar apparatus in acidic solution dissociated the flagellar filaments, yielding hook-basal body complexes with and without the associated disks. If whole cells were subjected to low pH, double disks of the same diameter and with a central hole of about 13 nm could be isolated. Similar parallel disks could be seen also in negatively stained whole cells. When uranyl acetate was used for negative staining of the intact flagella, concentric rings were detected on the disks, similar to the concentric membrane rings found by Coulton and Murray (J. W. Coulton and R. G. E. Murray, J. Bacteriol. 136:1037-1049, 1978) on platelike arrays of proteins in outer membrane preparations of Aquaspirillum serpens. Because the disks of W. succinogenes can be isolated together with the flagellar hook-basal body complex, they appear to be basal-body-rather than secondary membrane-associated structures. It is possible that these disks are the bearing or stator of this rotary device.

Population studies.

PIP: A number of studies have been published about the population change in New Zealand between 1936 and 1986. During this time an intellectual and demographic revolution also took place simultaneously. From 1961 and 1986 the population increased from 2.4 million to 3.3 million mainly because of Polynesian immigration, and the elderly and females also increased. The Maoris became urban. Mortality stayed stable, but fertility declined to below replacement level in the 1980's. Murray Wilson (1988) analyzed the relationship of fertility and mortality in terms of a post-transitional (developed) society like Australia or the European norm of a 2-children family. In his view the youth culture, television, and female aspirations greatly influenced childrearing. Daniel Noin (1988) examined the current state of geographical research on mortality from a methodological point of view finding wide differences in mortality in Quebec, Brussels, Paris, and London attributable to culture. Mosley and Chen (1984) argued that social, cultural, and economic factors to mortality are mediated by individual, household, and community variables. Jones and Moon (1987) dealt with medical geography in the context of the consumption of health care and disease ecology. Momsen and Townsend (1987) addressed the role of women in developing countries stating that gender is socially created and it examined the worldwide subordination of women. In the 1980's a host of other authors have also touched on the subject of the demography of gender analyzing discrimination against female children, unmarried American women, and single parent families. Yet Fahey (1988) stated that gender was only regarded relevant by Australian geographers as a demographic variable.^ieng

Experimental study to evaluate nursing staff morale in a high stimulation geriatric psychiatry setting.

This work is part of a larger study carried out at Murray Royal Hospital, Perth, Scotland. The study was concerned with staff morale under two distinct management approaches towards the care of the elderly patient with dementia. In one ward a dynamic, high stimulation approach was adopted including reality orientation, reminiscence therapy and socialization therapy. This was run in a well-structured manner by the ward staff and other agents. The other ward had no such structure imposed and was left to carry out 'traditional' nursing care to a high standard. The tool used to collect the data was a precoded questionnaire developed by Jones & Galliard. The hypothesis on which the study was based was as follows, 'that morale is increased in nursing staff involved in a high stimulation ward environment for the hospitalized elderly patient with dementia'. The findings of the study appear to indicate that the hypothesis is supported. It appears that the imposition of the structured high stimulation management approach was responsible for such an outcome and that nursing staff respond well to such an approach.