Rapid detection of sepsis using cesda: the caenorhabditis elegans sepsis detection assay
Rev. Soc. Bras. Med. Trop
; 52: e20180300, 2019. tab, graf
Article
in En
| LILACS
| ID: biblio-1041561
Responsible library:
BR1.1
ABSTRACT
Abstract INTRODUCTION The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS CESDA could be further explored for sepsis diagnosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Biomarkers
/
Chemotaxis
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Caenorhabditis elegans
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Sepsis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
MEDICINA TROPICAL
Year:
2019
Document type:
Article
Affiliation country:
Country of publication:
BR
/
BRASIL
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BRASILE
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BRAZIL
/
BRESIL