Screening of serum marker proteins in osteosarcoma and preliminary bioinformatic analysis on POLR3F / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 836-841, 2011.
Article
in Zh
| WPRIM
| ID: wpr-320126
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen potential serum marker proteins of osteosarcoma, and to make a preliminary bioinformatics analysis of RNA polymerase III polypeptide F (POLR3F).</p><p><b>METHODS</b>Gene chip and SELDI-TOF MS was used to screen genes differentially expressed in osteosarcoma. The associations of potential biomarkers from SELDI data and microarray analysis were further inferred by link-test to identify biomarkers that could likely be used for diagnosis. MATLAB was used to search transcription factors binding site in the promoter region and miRNAs binding site in 3'-UTR of POLR3F, respectively.</p><p><b>RESULTS</b>653 differentially expressed genes were found in osteosarcoma cells, while six differentially expressed protein peaks with significant statistical significances were detected by SELDI-TOF MS in patient's serum. 13 potential biomarkers for early diagnosis of osteosarcoma were screened by link-test. A conserved STAT3 binding site and a miRNA target site were found in proximal promoter regions and 3'-UTR region of POLR3F, respectively.</p><p><b>CONCLUSIONS</b>Link-test is a effective method to identify osteosarcoma biomarkers from both microarray and SELDI-TOF MS database. The results confirmed that POLR3F may be a promising biomarker for early diagnosis and a therapy target of osteosarcoma.</p>
Full text:
1
Database:
WPRIM
Main subject:
Peptides
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Protein Binding
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Binding Sites
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Blood
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Bone Neoplasms
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RNA Polymerase III
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RNA, Messenger
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Blood Proteins
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Biomarkers, Tumor
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Osteosarcoma
Type of study:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Limits:
Adolescent
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Adult
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Child
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Female
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Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2011
Document type:
Article