Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;34(9): 1175-1183, Sept. 2001. ilus, graf
Article
in En
| LILACS
| ID: lil-290404
Responsible library:
BR1.1
ABSTRACT
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Angiotensin II
/
Receptors, Angiotensin
/
CHO Cells
/
Endocytosis
Limits:
Animals
Language:
En
Journal:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
2001
Document type:
Article
Affiliation country:
Country of publication: