A mechanism of resistance to HIV-1 entry: inefficient interactions of CXCR4 with CD4 and gp120 in macrophages.
Virology
; 259(1): 1-6, 1999 Jun 20.
Article
in En
| MEDLINE
| ID: mdl-10364484
ABSTRACT
To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
CD4 Antigens
/
HIV Envelope Protein gp120
/
Acquired Immunodeficiency Syndrome
/
HIV-1
/
Receptors, CXCR4
/
Macrophages
Limits:
Humans
Language:
En
Journal:
Virology
Year:
1999
Document type:
Article
Affiliation country: