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Cultured rat microglia express functional beta-chemokine receptors.
Boddeke, E W; Meigel, I; Frentzel, S; Gourmala, N G; Harrison, J K; Buttini, M; Spleiss, O; Gebicke-Härter, P.
Affiliation
  • Boddeke EW; Department of Medical Physiology, University of Groningen, The Netherlands. h.w.g.m.boddeke@med.rug.nl
J Neuroimmunol ; 98(2): 176-84, 1999 Aug 03.
Article in En | MEDLINE | ID: mdl-10430051
ABSTRACT
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia.
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Collection: 01-internacional Database: MEDLINE Main subject: Microglia / Receptors, Chemokine Limits: Animals Language: En Journal: J Neuroimmunol Year: 1999 Document type: Article Affiliation country:
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Microglia / Receptors, Chemokine Limits: Animals Language: En Journal: J Neuroimmunol Year: 1999 Document type: Article Affiliation country: