Cultured rat microglia express functional beta-chemokine receptors.
J Neuroimmunol
; 98(2): 176-84, 1999 Aug 03.
Article
in En
| MEDLINE
| ID: mdl-10430051
ABSTRACT
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microglia
/
Receptors, Chemokine
Limits:
Animals
Language:
En
Journal:
J Neuroimmunol
Year:
1999
Document type:
Article
Affiliation country: