The COP9/signalosome complex is conserved in fission yeast and has a role in S phase.
Curr Biol
; 9(23): 1427-30, 1999 Dec 02.
Article
in En
| MEDLINE
| ID: mdl-10607571
ABSTRACT
The COP9/signalosome complex is conserved from plant to mammalian cells. In Arabidopsis, it regulates the nuclear abundance of COP1, a transcriptional repressor of photomorphogenic development [1] [2]. All COP (constitutive photomorphogenesis) mutants inappropriately express genes that are normally repressed in the dark. Eight subunits (Sgn1-Sgn8) of the homologous mammalian complex have been purified [3] [4]. Several of these have been previously identified through genetic or protein interaction screens. No coherent model for COP9/signalosome function has yet emerged, but a relationship with cell-cycle progression by transcriptional regulation, protein localisation or protein stability is possible. Interestingly, the COP9/signalosome subunits possess domain homology to subunits of the proteasome regulatory lid complex [5] [6]. Database searches indicate that only Sgn5/JAB1 is present in Saccharomyces cerevisiae, precluding genetic analysis of the complex in cell-cycle regulation. Here we identify a subunit of the signalosome in the fission yeast Schizosaccharomyces pombe through an analysis of the DNA-integrity checkpoint. We provide evidence for the conservation of the COP9/signalosome complex in fission yeast and demonstrate that it functions during S-phase progression.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Schizosaccharomyces
/
Signal Transduction
/
Proteins
/
S Phase
Limits:
Humans
Language:
En
Journal:
Curr Biol
Journal subject:
BIOLOGIA
Year:
1999
Document type:
Article
Affiliation country: