Large-scale functional analysis using peptide or protein arrays.
Nat Biotechnol
; 18(4): 393-7, 2000 Apr.
Article
in En
| MEDLINE
| ID: mdl-10748518
ABSTRACT
The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells. Comprehensive sets of purified peptides and proteins permit high-throughput screening for discrete biochemical properties, whereas formats involving living cells facilitate large-scale genetic screening for novel biological activities. In the past year, three major genome-scale studies using yeast as a model organism have investigated different aspects of protein function, including biochemical activities, gene disruption phenotypes, and protein-protein interactions. Such studies show that protein arrays can be used to examine in parallel the functions of thousands of proteins previously known only by their DNA sequence.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Proteins
/
Peptide Library
Language:
En
Journal:
Nat Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2000
Document type:
Article
Affiliation country: