A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation.
J Infect Dis
; 181(4): 1467-70, 2000 Apr.
Article
in En
| MEDLINE
| ID: mdl-10753727
ABSTRACT
In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria deli bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from deli ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed viral contamination of a food item likely to have been contaminated by a food handler.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Disease Outbreaks
/
Norwalk virus
/
Caliciviridae Infections
/
Foodborne Diseases
/
Gastroenteritis
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Infant
/
Male
Country/Region as subject:
America do norte
Language:
En
Journal:
J Infect Dis
Year:
2000
Document type:
Article
Affiliation country: