Antizyme regulates the degradation of ornithine decarboxylase in fission yeast Schizosaccharomyces pombe. Study in the spe2 knockout strains.
J Biol Chem
; 276(24): 21235-41, 2001 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-11283013
ABSTRACT
The mechanism of the regulatory degradation of ornithine decarboxylase (ODC) by polyamines was studied in fission yeast, Schizosaccharomyces pombe. To regulate cellular spermidine experimentally, we cloned and disrupted S-adenosylmethionine decarboxylase gene (spe2) in S. pombe. The null mutant of spe2 was devoid of spermidine and spermine, accumulated putrescine, and contained a high level of ODC. Addition of spermidine to the culture medium resulted in rapid decrease in the ODC activity caused by the acceleration of ODC degradation, which was dependent on de novo protein synthesis. A fraction of ODC forming an inactive complex concomitantly increased. The accelerated ODC degradation was prevented either by knockout of antizyme gene or by selective inhibitors of proteasome. Thus, unlike budding yeast, mammalian type antizyme-mediated ODC degradation by proteasome is operating in S. pombe.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ornithine Decarboxylase
/
Polyamines
/
Adenosylmethionine Decarboxylase
/
Schizosaccharomyces
/
Proteins
Language:
En
Journal:
J Biol Chem
Year:
2001
Document type:
Article
Affiliation country: