Characterization of a microsomal retinol dehydrogenase gene from amphioxus: retinoid metabolism before vertebrates.
Chem Biol Interact
; 130-132(1-3): 359-70, 2001 Jan 30.
Article
in En
| MEDLINE
| ID: mdl-11306058
ABSTRACT
Amphioxus, a member of the subphylum Cephalochordata, is thought to be the closest living relative to vertebrates. Although these animals have a vertebrate-like response to retinoic acid, the pathway of retinoid metabolism remains unknown. Two different enzyme systems - the short chain dehydrogenase/reductases and the cytosolic medium-chain alcohol dehydrogenases (ADHs) - have been postulated in vertebrates. Nevertheless, recent data show that the vertebrate-ADH1 and ADH4 retinol-active forms originated after the divergence of cephalochordates and vertebrates. Moreover, no data has been gathered in support of medium-chain retinol active forms in amphioxus. Then, if the cytosolic ADH system is absent and these animals use retinol, the microsomal retinol dehydrogenases could be involved in retinol oxidation. We have identified the genomic region and cDNA of an amphioxus Rdh gene as a preliminary step for functional characterization. Besides, phylogenetic analysis supports the ancestral position of amphioxus Rdh in relation to the vertebrate forms.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinoids
/
Chordata, Nonvertebrate
/
Alcohol Oxidoreductases
Limits:
Animals
Language:
En
Journal:
Chem Biol Interact
Year:
2001
Document type:
Article
Affiliation country: