Role of bursin in the development of B lymphocytes in chicken embryonic Bursa of Fabricius.
Dev Comp Immunol
; 25(5-6): 485-93, 2001.
Article
in En
| MEDLINE
| ID: mdl-11356228
ABSTRACT
Localization and role of bursin during Bursa of Fabricius (BF) ontogeny were examined by immunohistochemical staining and by in ovo injection with anti-bursin antibody. Mouse monoclonal anti-bursin antibody HU2 was generated by immunization with synthetic bursin. It recognized reticular cells (REC), follicular associated epithelium (FAE), FAE-supporting cells, and the basal layer of interfollicular epithelium (IFE) in the mature BF. Bu-1(+) cells were first detectable in the mesenchyme area at 13 days of embryogenesis (E13) before bud formation, then lined up along the bud, and homed into the bud at around E15. IgM(+) cells were detected in the bud after E13. Bursin was first observed at the under edge of the bud. Injection of HU2 into embryonal vein at E13 suppressed the appearance of IgM(+) cells in the Bursa at E17. These results indicate that bursin exists beneath the bud and may act on the appearance of IgM(+) cells during BF ontogeny.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligopeptides
/
Bursa of Fabricius
/
B-Lymphocytes
Limits:
Animals
Language:
En
Journal:
Dev Comp Immunol
Year:
2001
Document type:
Article
Affiliation country: