Determination of carboxylic acids, carbohydrates, glycerol, ethanol, and 5-HMF in beer by high-performance liquid chromatography and UV-refractive index double detection.
J Chromatogr Sci
; 39(6): 235-8, 2001 Jun.
Article
in En
| MEDLINE
| ID: mdl-11396687
ABSTRACT
A high-performance liquid chromatographic method is proposed for the simultaneous separation of main carboxylic acids, carbohydrates, ethanol, glycerol, and 5-HMF in beer by direct injection. A column packed with a sulfonated divinyl benzene-styrene copolymer and an isocratic elution with 0.0045N sulfuric acid and acetonitrile (6%, v/v) are employed. UV and refractive index detectors connected in series are also used to reduce the matrix interference of phenolic compounds. In conditions described, nine compounds are quantitated in a single chromatographic run without any pretreatment except for sample dilution and filtration before injection. Precision, accuracy, linearity of response, limit of detection, and limit of quantitation are also evaluated for each compound. Satisfactory results are obtained to justify the application of this method to all phases of beer production for process and quality control.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beer
/
Carbohydrates
/
Carboxylic Acids
/
Chromatography, High Pressure Liquid
/
Ethanol
/
Furaldehyde
/
Glycerol
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Chromatogr Sci
Year:
2001
Document type:
Article
Affiliation country: