[Analysis of the structure-functional features of a highly-repeating centromere sequence from the Fringilla coelebs L. (Aves: Passeriformes) genome]. / Analiz strukturno-funktsional'nykh osobennoste vysokopovtoriaiushcheisia tsentromernoi posledovatel;nosti iz genoma Fringilla coelebs L. (Aves: Passeriformes).

ABSTRACT

A highly repetitive centromeric Fringilla coelebs PstI (FCP) element was cloned and sequenced. The FCP tandem repeats with unit 505 or 506 nt accounted for about 0.9% of the entire genome and had 57% GC. Direct genomic sequencing with FCP-specific primers and ThermoFidelase 2A revealed the consensus sequence and the five most common single-nucleotide polymorphisms (SNPs) for the FCP unit. FCP may be transcribed and may play a role in spatial arrangement of the genome.