Endogenous beta-galactosidase activity in amphioxus: a useful histochemical marker for the digestive system.
Dev Genes Evol
; 211(3): 154-6, 2001 Mar.
Article
in En
| MEDLINE
| ID: mdl-11455428
ABSTRACT
Endogenous beta-galactosidase activity has been shown in the digestive tract of amphioxus from the larval to the adult stage and it can be easily followed as a histochemical marker. Enzymatic activity first appeared in 30-h larvae, became evident in 36-h larvae and remained in adults. In situ detection of beta-galactosidase activity was used to monitor morphological and functional differentiation of the digestive system and the posteriorization of the endodermal structures in retinoic-acid treated embryos. The endogenous beta-galactosidase activity was distinguished from the bacterial lacZ reporter by incubation at low pH.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chordata, Nonvertebrate
/
Beta-Galactosidase
/
Digestive System
Limits:
Animals
Language:
En
Journal:
Dev Genes Evol
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2001
Document type:
Article
Affiliation country: