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Endogenous beta-galactosidase activity in amphioxus: a useful histochemical marker for the digestive system.
Cañestro, C; Albalat, R; Escrivà, H; Gonzàlez-Duarte, R.
Affiliation
  • Cañestro C; Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 645 Av. Diagonal, 08028 Barcelona, Spain. roser@bio.ub.es
Dev Genes Evol ; 211(3): 154-6, 2001 Mar.
Article in En | MEDLINE | ID: mdl-11455428
ABSTRACT
Endogenous beta-galactosidase activity has been shown in the digestive tract of amphioxus from the larval to the adult stage and it can be easily followed as a histochemical marker. Enzymatic activity first appeared in 30-h larvae, became evident in 36-h larvae and remained in adults. In situ detection of beta-galactosidase activity was used to monitor morphological and functional differentiation of the digestive system and the posteriorization of the endodermal structures in retinoic-acid treated embryos. The endogenous beta-galactosidase activity was distinguished from the bacterial lacZ reporter by incubation at low pH.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Chordata, Nonvertebrate / Beta-Galactosidase / Digestive System Limits: Animals Language: En Journal: Dev Genes Evol Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2001 Document type: Article Affiliation country:
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Chordata, Nonvertebrate / Beta-Galactosidase / Digestive System Limits: Animals Language: En Journal: Dev Genes Evol Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2001 Document type: Article Affiliation country: