Genetic selection of regulatory mutants of mammalian adenylyl cyclases.
Methods Enzymol
; 345: 241-51, 2002.
Article
in En
| MEDLINE
| ID: mdl-11665608
ABSTRACT
Initial steps in the identification of the Gs alpha-binding site present in mammalian adenylyl cyclases can be achieved with the use of the yeast genetic system described. It must be stressed that this system serves as a means to identify mutants that are candidates; biochemical analysis of these mutants is a next and necessary step in the confirmation of these phenotypes. The system described can be readily adapted for the isolation of additional classes of mammalian adenylyl cyclase mutants including mutants with altered regulation toward forskolin, catalytic abnormalities, or enhanced sensitivities toward activators. In addition, this system can be employed for the isolation of constitutively active adenylyl cyclase mutants, or by coexpressing other adenylyl cyclase isoforms and their known regulators, mutations in the binding sites for these molecules can be elucidated.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenylyl Cyclases
/
Mutation
Limits:
Animals
Language:
En
Journal:
Methods Enzymol
Year:
2002
Document type:
Article
Affiliation country: