7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine (NECA) as new fluorescent probes for human A(3) adenosine receptors.
Bioorg Med Chem Lett
; 11(23): 3023-6, 2001 Dec 03.
Article
in En
| MEDLINE
| ID: mdl-11714602
ABSTRACT
New fluorescent ligands for adenosine receptors (ARs), obtained by the insertion, in the N(6) position of NECA, of NBD-moieties with linear alkyl spacers of increasing length, proved to possess a high affinity and selectivity for the A(3) subtype expressed in CHO cells. In fluorescence microscopy assays, compound 2d, the most active and selective for human A(3)-AR, permitted visualization and localization of this human receptor subtype, showing its potential suitability for internalization and trafficking studies in living cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Purinergic P1
/
Adenosine-5'-(N-ethylcarboxamide)
/
Fluorescent Dyes
Limits:
Animals
/
Humans
Language:
En
Journal:
Bioorg Med Chem Lett
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2001
Document type:
Article
Affiliation country: