Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: fluorimetric, immunohistochemical, and Western blot studies.
Epilepsia
; 43 Suppl 5: 227-9, 2002.
Article
in En
| MEDLINE
| ID: mdl-12121326
ABSTRACT
PURPOSE:
This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.METHODS:
ATP-mediated changes in intracellular calcium were studied by the fura-2 method. Immunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.RESULTS:
The fluorimetric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. The Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.CONCLUSIONS:
These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pilocarpine
/
Adenosine Triphosphate
/
Convulsants
/
Epilepsy, Temporal Lobe
Limits:
Animals
Language:
En
Journal:
Epilepsia
Year:
2002
Document type:
Article
Affiliation country: