Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study.
Biomol Eng
; 19(2-6): 119-24, 2002 Aug.
Article
in En
| MEDLINE
| ID: mdl-12202171
ABSTRACT
Blocks of two porous synthetic hydroxyapatites (HA) with porosity fraction of 30-40 and 50-60 vol%, respectively and a coralline derived porous HA were evaluated in vitro in presence of the osteogenic line MC3T3-E1 and of L929 fibroblasts. The two tested biomaterials did not affect cellular proliferation (MTT test), but the contact inhibited alkaline phosphatase activity. Porous aggregates resulted perfectly biocompatible in the tests performed, since observations performed by light microscopy did not show any cell morphological change, osteoblast presented a stellar shape and typical pseudopodes. SEM observations showed intercellular matrix containing fibers on HA-based porous aggregates.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Materials Testing
/
Ceramics
/
Durapatite
/
Fibroblasts
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Biomol Eng
Journal subject:
BIOLOGIA MOLECULAR
/
ENGENHARIA BIOMEDICA
Year:
2002
Document type:
Article
Affiliation country: